This thesis focuses on the design of new high pressure injection system application on diesel engine. The design came from company Zetor’s demands. For this application was chosen system Common Rail supplied by company Bosch. Assignment was separated into several tasks. Research of suitable components for low pressure section, design of waste system and high pressure injection tubes design including injector clamping. Due to injectors used in this system was necessary to redesign cover for valves and even new system to lead wires from outside of the engine to injectors. Injector is attached by screw connection, which is composed from screw and clamping jaw. These parts are subjected to an analytic calculations. Clamping jaw of injector is even subjected to a FEM analysis, which focuses on fatigue life. Finally, the drawings were documented.

This work deals with frequency generators based on the direct digital synthesis method DDS. Basic principles and attributes of the frequency generator DDS are explained. The text describes parameters influencing and defining a quality of the generated signal. The list of available integrated circuits realizing the direct digital synthesis is mentioned. A construction of the DDS generator with a device AD9954 and the generator control are described. At the end of the work, the function and parameters of the designed generator are verified.

This thesis is an engineering design of an intelligent fast-charger for lead-acid accumulators. It contains calculations of each component. The switching power supply is the mainstay of the charger that makes the charger portable, lightweight and small. The Intelligent fast-charger for lead-acid accumulators charges the accumulator by method of constant voltage with current limitation of 0,5A, 5A, 10A, 50A. 50A current is used in winter during starting of a car, when the battery can´t provide sufficient current. There are three LEDs placed on the front side of the charger reflecting status of the device. The device comprises safety protection for the charger, supply network and battery.

This thesis deals with energy self-sufficiency focusing on the independence of the electric grid. Basic line emanating project form a concrete proposal for autonomous energy supply system. Emphasis is placed primarily on photovoltaic systems, electric energy accumulation and selection of individual components of such a system. Another important part of the thesis is also an economic evaluation of design variations. The thesis also deals with a market research, and thereof derived benefit assessment of each technology. The accompanying chapters are then devoted to the history of autonomous life style or classification of buildings according to their energy performance.

The work deals with relatively new components allowing electric energy accumulation – ultracapacitors. It focuses on their application in traffic systems – in independent electric vehicles. Design and verification of a system with ultracapacitor and DC/DC adaptive converter was done. Control of the adaptive converter modifies very positively the time wafeform of the traction accumulator current during the drive cycle. The designed connection of ultracapacitor and DC/DC converter implemented in the drive structure of experimental electric vehicle with induction machine contributes to increment the action radius of the vehicle by 16% (determined by experimental verification). This result was achived particularly by limitation of traction accumulator current peaks, And by more effective storage of energy gained by recuperative braking of the vehicle as well. The core of the system is a control of the adaptive converter in order to provide an active filtration of the accumulator’s current to its long-period mean value, i.e. elimination of current (power) peaks. These are caused by acceleration from non-zero initial vehicle speed or by recuperative braking. This is done by a subsidiary current loop. The converter has a superior voltage regulation loop, which sets in long-time period the voltage of ultracapacitors to the proper value – indirectly dependent on the speed of the vehicle. This ensures the appropriate energy management of the ultracapacitor. In the following, properties of test set of ultracapacitors were verified. Finally, methods of suppression of capacity variability influence in series connection of these components were compiled and critically reviewed.

This diploma thesis deals with photovoltaic rechargable regulators used in isolated, or off-grid photovoltaic systems. First, basic types of off-grid installations, including their functions and applications in practice are described. Then, possibilities of electric energy accumulation in photovoltaic systems are mentioned, considering actual, accesible technologies. Matters of electric energy accumulation in leaden accumulators are examined in detail. Main part of the diploma thesis is about electronic designs of photovoltaic, also called solar, rechargable regulators. These are the main connecting part between photovoltaic panels, accumulator, but also often connected charge, which is appliance. Individual electronic regulators concepts are described narrowly, then compared and evaluated in thesis´ conclusion.

Le cancer de la vessie est un cancer fréquent et extrêmement onéreux par patient puisque plusieurs patients subissent des récidives de cancer et ont recours parfois à des chirurgies complexes. Il est donc important de diagnostiquer efficacement ces cancers lors de la prise en charge initiale du patient. En effet, la procédure standard d’imagerie pour la détection du cancer est la cystoscopie de la vessie guidée par lumière blanche, toutefois cette méthode ne permet pas de bien distinguer les cellules qui sont propices à l’invasion musculaire des cellules de cancer de la vessie non infiltrant. Ce mémoire propose d’utiliser un nouvel immunoconjugué fluorescent ciblant la sous-unité alpha du récepteur de l’interleukine 5, un nouveau biomarqueur spécifique aux cellules du cancer de la vessie infiltrant, afin d’effectuer la cystoscopie de la vessie guidée par fluorescence. Pour ce faire, un protocole de conjugaison du fluorochrome cyanine-5 (Cy5) à un anticorps monoclonal a été développé. De plus, un protocole de conjugaison d’un peptide Cell Accumulator (ACCUM) sur cet anticorps fluorescent (A14-Cy5-ACCUM) a été optimisé. Ensuite, la capacité de cet immunoconjugué à marquer les cellules humaines de cancer de la vessie infiltrantes du muscle (MIBC), HT1376, a été testée. Par la suite, un nouveau modèle orthotpique murin de MIBC humain permettant la validation préclinique prochaine de l’A14-Cy5-ACCUM a été développé. Une banque de plasma et sérum sanguin, et d’urine de patients sains et atteints de cancer de la vessie a été compilé. Cette biobanque contient 111 échantillons de plasma sanguin et d’urine qui pourront être utilisé afin de tester l’hypothèse selon laquelle le niveau d’interleukine-5 sanguin pourrait être un facteur pronostique pour la progression du cancer de la vessie. Ce projet jette les bases pour l’évaluation potentielle de la cystoscopie guidée par fluorescence lors de la prise en charge initiale des patients atteints de cancer de la vessie afin d’améliorer la survie sans progression et la survie à long terme des patients atteints de MIBC. / Abstract: Bladder cancer is a frequent and extremely costly cancer when evaluated on a per-patient basis because of its high recurrence rate and patients undergoing complex medical procedures. It is of utmost importance to better identify the aggressiveness of this cancer at initial diagnosis. The standard procedure for bladder cancer detection is still white-light guided cystoscopy, which relies mostly on physicians experience in regard to identifying invasive malignancies. This memoir proposes the use of a new fluorescent immunoconjugate, targeting the alpha subunit of interleukin-5 receptor (IL-5R[apha]), a new biomarker specific to muscle-invasive bladder cancer (MIBC) cells for fluorescence-guided cystoscopy. To do so, a conjugation protocol to fluorescently label a monoclonal antibody with cyanine-5 fluorophores has been developped. Then, a conjugation protocol to attach Cell Accumulator (ACCUM) peptides to this fluorescent immunoconjugate (A14-Cy5-ACCUM) has been optimized. Moreover, the ability of A14-Cy5-ACCUM to stain MIBC cell line HT1376 has been tested. Most importantly, a novel orthotpic rat model of human MIBC for the future preclinical validation of fluorescence-guided cystoscopy in rat bladder has been developped. Finally, a new bladder cancer tissue repository at the CHUS has been established. This repository contains a total of 111 plasma and urine patient samples that will be helpful to evaluate if interleukin-5 blood levels could be used as a prognosis marker for bladder cancer progression. This project laid the basis for the potential evaluation of fluorescence-guided cystoscopy during initial diagnosis of bladder cancer patients to improve their disease-free and long-term survival.

The purpose of this diploma thesis is to describe the impact of compaction pressure on the electrochemical parameters of lithium-sulfur batteries. Theoretical part of this thesis contains briefly described terminology and general issues of batteries and their division. Every kind of battery is provided with a closer description of a specific battery type. A separate chapter is dedicated to lithium cells, mainly lithium-ion batteries. Considering various composition of lithium-ion batteries, this chapter deeply analyzes mostly used active materials of electrodes, used electrolytes and separators. Considering that the electrochemical principle of Li-S and Li-O batteries is different to Li-ion batteries, these accumulators of new generation are included in individual subhead. In the experimental part of this thesis are described methods used to measure electrochemical parameters of Li-S batteries. Next chapter contains description of preparing individual electrodes and their composition. Rest of the experimental part of my thesis is dedicated to the description of individual experiments and achieved results.