One of the main causes of premature mortality is decreased physical activity. The purpose of the study is to investigate, based on self-determination theory, the motives for choosing active transportation in the form of an electric bike instead of passive transportation such as bus or car among participants in the Elcyklist project. A qualitative research method was used, and 17 semi-structured interviews were conducted based on a motivational perspective. The results showed that the motive for electric biking was the experience of independence, challenge, community, and inspiration, which represented a sense of well-being. Additionally, enjoyment, environmentally, economically, and health were motives for electric biking that represented benefits in everyday life. Participants' choice to use electric bikes satisfied their basic needs for autonomy, competence, and relatedness. Furthermore, the study showed that the participants were motivated to use the electric bike mainly by self-determined motivation. The main conclusion is the importance to address and promote self-determined motivation to support and maintain motivation healthy behaviors and lifestyles. The study showed that the opportunity to borrow an e-bike as offered in the Electric Biking project can be a good way to promote self-determined motivation for active transportation as well as health-promoting behaviors.

Grande parte da comunidade internacional defende que as cidades devem ser sustentáveis, inclusivas, seguras e resilientes. Neste sentido, tem sido desenvolvidas diversas iniciativas que, normalmente, envolvem revitalização de áreas urbanas, implementação de infraestrutura e criação de políticas de incentivo ao transporte não motorizado, como o deslocamento a pé. Apesar das evidentes vantagens deste tipo de transporte, como o baixo impacto ambiental e a prática de exercícios físicos, a vulnerabilidade dos pedestres é maior devido a sua exposição direta à fatores externos. Essa realidade se agrava quando são consideradas regiões de trânsito no entorno de escolas, colégios ou outras instituições educacionais, àreas estratégicas por atrairem novos usuários para a rede local de tráfego, incluindo as crianças. Esta dissertação trata sobre projeto de um aplicativo para promover o deslocamento de pedestres em áreas escolares, estimulando o transporte ativo entre crianças e adolescentes. A metodologia de projeto utilizada para a criação do sistema teve como base teórica e prática o design de interação, e considerou paralelamente as pesquisas específicas sobre o deslocamento de pedestres em áreas escolares obtidas na revisão teórica. A usabilidade e o potencial do sistema para o incentivo ao transporte ativo foram validados através de testes com um protótipo semi-funcional e de discussões orientadas em grupo focado. Os resultados obtidos nos testes foram majoritariamente favoráveis à adesão ao uso do aplicativo e ao seu potencial para incentivo à realização de deslocamentos a pé no trajeto casa/escola, de acordo com diversos critérios de avaliação fundamentados na revisão teórica. / A large part of the international community argues that cities must be sustainable, inclusive, secure and resilient. In this sense, several initiatives have been developed that involve revitalization of urban areas, implementation of infrastructure and creation of policies to encourage non-motorized transportation, such as walking. Despite the obvious advantages of this type of transport, such as low environmental impact and physical exercise, the vulnerability of pedestrians is greater due to their direct exposure to external factors. This reality worsens when they are considered transit regions around schools, colleges or other educational institutions, strategic areas for attracting new users to the local traffic network, including children. This thesis deals with the design of a mobile application to promote the movement of pedestrians in school areas to stimulating active transportation among children and adolescents. The project methodology used for the creation of the system was based on interaction design, and considered the specific research on the pedestrian movement in school areas carried out in the theoretical revision. The results obtained in the tests were mostly positive according to several evaluation criteria based on the theoretical review, indicating the adherence to the use of the proposed mobile app and showing its potential as an incentive children and teenagers walking.

Chan, Wai Hon. / Thesis (M.Phil.)--Chinese University of Hong Kong, 2010. / Includes bibliographical references (leaves 134-141). / Abstracts in English and Chinese. / Acknowledgements --- p.2 / Abstract --- p.3 / 摘要 --- p.5 / Content --- p.6 / List of Abbreviations and symbols --- p.10 / Chapter Chapter 1 --- Introduction --- p.13 / Chapter 1.1 --- The Severity of Influenza A Virus --- p.14 / Chapter 1.2 --- Introduction to Influenza A Virus --- p.15 / Chapter 1.3 --- What is Nucleoprotein? --- p.17 / Chapter 1.4 --- Multifunctional role of Nucleoprotein --- p.19 / Chapter 1.4.1 --- Interaction of Nucleoprotein with Other Viral Components --- p.19 / Chapter 1.4.1 --- Interaction of Nucleoprotein with Cellular Components --- p.22 / Chapter 1.5 --- Aims of study --- p.23 / Chapter Chapter 2 --- Materials and Methods / Chapter 2.1 --- Materials --- p.25 / Chapter 2.1.1 --- Chemical reagents --- p.25 / Chapter 2.1.2 --- Buffers --- p.28 / Chapter 2.1.2.1 --- Preparation of Buffers --- p.28 / Chapter 2.1.2.2 --- Buffer for Common Use --- p.28 / Chapter 2.1.3 --- Plasmids and Strains --- p.31 / Chapter 2.2 --- Methods --- p.32 / Chapter 2.2.1 --- Molecular Cloning --- p.32 / Chapter 2.2.2 --- "Expression of the Recombinant NP-WT/ mutants and importin α1, 3and 5 in E.coli" --- p.46 / Chapter 2.2.3 --- Purification of the NP WT/ variants --- p.50 / Chapter 2.2.4 --- "Purification of importin αl, 3 and 5" --- p.53 / Chapter 2.2.5 --- In vitro interaction study between NP and importin α --- p.56 / Chapter 2.2.6 --- In vivo interaction study between NP and importin α --- p.59 / Chapter 2.2.7 --- In vivo analysis to study NP-NP homo-oligomerization --- p.64 / Chapter 2.2.8 --- In vitro static light scattering analysis to determine NP oligomeric state --- p.68 / Chapter 2.2.9 --- Control experiments to verify NP-polymerases and NP-RNA interaction --- p.69 / Chapter Chapter 3 --- Functional analysis of influenza H5N1 nucleoprotein tail loop for oligomerization and ribonucleoprotein activities / Chapter 3.1 --- Introduction --- p.72 / Chapter 3.2 --- Results --- p.75 / Chapter 3.2.1 --- Tail loop insertion is maintained by intra- and inter-molecular interactions --- p.75 / Chapter 3.2.2 --- NP mutants display defective transcription-replication activity --- p.77 / Chapter 3.2.3 --- Expression and purification of defective NP variants --- p.80 / Chapter 3.2.4 --- Defective NP variants interact with RNA and the polymerase complex --- p.85 / Chapter 3.2.5 --- Defective NP variants possess abnormal oligomeric states in vitro --- p.91 / Chapter 3.2.6 --- NP variants with impaired RNP activity cannot form homo-oligomers in vivo --- p.95 / Chapter 3.3 --- Discussion --- p.98 / Chapter Chapter 4 --- Biophysical characterization of the interaction between influenza nucleoprotein and importin α / Chapter 4.1 --- Introduction --- p.105 / Chapter 4.2 --- Results --- p.108 / Chapter 4.2.1 --- Expression and Purification of NP-WT/ NLS variants and Importin a --- p.108 / Chapter 4.2.2 --- Pull down Assay --- p.113 / Chapter 4.2.3 --- Light scattering analysis (NP-lmportin α5) --- p.114 / Chapter 4.2.4 --- Binding assay of NP NLS variants with importin α5 --- p.115 / Chapter 4.2.5 --- BIAcore 3000 Surface Plasmon Resonance (NP-lmportin α5) --- p.118 / Chapter 4.2.6 --- QRT-PCR (NP-lmportin a5) --- p.123 / Chapter 4.3 --- Discussion --- p.125 / References --- p.134

Thesis (Ph. D.)--University of Virginia, 2008. / Title from title page. Includes bibliographical references. Also available online through Digital Dissertations.

Grande parte da comunidade internacional defende que as cidades devem ser sustentáveis, inclusivas, seguras e resilientes. Neste sentido, tem sido desenvolvidas diversas iniciativas que, normalmente, envolvem revitalização de áreas urbanas, implementação de infraestrutura e criação de políticas de incentivo ao transporte não motorizado, como o deslocamento a pé. Apesar das evidentes vantagens deste tipo de transporte, como o baixo impacto ambiental e a prática de exercícios físicos, a vulnerabilidade dos pedestres é maior devido a sua exposição direta à fatores externos. Essa realidade se agrava quando são consideradas regiões de trânsito no entorno de escolas, colégios ou outras instituições educacionais, àreas estratégicas por atrairem novos usuários para a rede local de tráfego, incluindo as crianças. Esta dissertação trata sobre projeto de um aplicativo para promover o deslocamento de pedestres em áreas escolares, estimulando o transporte ativo entre crianças e adolescentes. A metodologia de projeto utilizada para a criação do sistema teve como base teórica e prática o design de interação, e considerou paralelamente as pesquisas específicas sobre o deslocamento de pedestres em áreas escolares obtidas na revisão teórica. A usabilidade e o potencial do sistema para o incentivo ao transporte ativo foram validados através de testes com um protótipo semi-funcional e de discussões orientadas em grupo focado. Os resultados obtidos nos testes foram majoritariamente favoráveis à adesão ao uso do aplicativo e ao seu potencial para incentivo à realização de deslocamentos a pé no trajeto casa/escola, de acordo com diversos critérios de avaliação fundamentados na revisão teórica. / A large part of the international community argues that cities must be sustainable, inclusive, secure and resilient. In this sense, several initiatives have been developed that involve revitalization of urban areas, implementation of infrastructure and creation of policies to encourage non-motorized transportation, such as walking. Despite the obvious advantages of this type of transport, such as low environmental impact and physical exercise, the vulnerability of pedestrians is greater due to their direct exposure to external factors. This reality worsens when they are considered transit regions around schools, colleges or other educational institutions, strategic areas for attracting new users to the local traffic network, including children. This thesis deals with the design of a mobile application to promote the movement of pedestrians in school areas to stimulating active transportation among children and adolescents. The project methodology used for the creation of the system was based on interaction design, and considered the specific research on the pedestrian movement in school areas carried out in the theoretical revision. The results obtained in the tests were mostly positive according to several evaluation criteria based on the theoretical review, indicating the adherence to the use of the proposed mobile app and showing its potential as an incentive children and teenagers walking.

Submitted by Bruna Rodrigues (bruna92rodrigues@yahoo.com.br) on 2016-09-28T14:32:58Z No. of bitstreams: 1 DissVLSO.pdf: 839524 bytes, checksum: 71c434ddb5669697de4e49af9bd3b7ba (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-10T19:05:51Z (GMT) No. of bitstreams: 1 DissVLSO.pdf: 839524 bytes, checksum: 71c434ddb5669697de4e49af9bd3b7ba (MD5) / Approved for entry into archive by Marina Freitas (marinapf@ufscar.br) on 2016-10-10T19:06:05Z (GMT) No. of bitstreams: 1 DissVLSO.pdf: 839524 bytes, checksum: 71c434ddb5669697de4e49af9bd3b7ba (MD5) / Made available in DSpace on 2016-10-10T19:06:18Z (GMT). No. of bitstreams: 1 DissVLSO.pdf: 839524 bytes, checksum: 71c434ddb5669697de4e49af9bd3b7ba (MD5) Previous issue date: 2016-04-13 / Não recebi financiamento / Brazil's cities in recent decades have a predominance of car use as children's mode of transport to school. In carrying out the route home-school by motorized modes children practice less physical activity to the detriment of health. Policies developed in schools can play an important role in determining how students travel to school. In this context, this research aims to identify the factors that influence the perception of managers about using the walk to access the school. The focuses of the research are the municipal and private elementary schools 1. The case study was conducted in the city of São José do Rio Preto. Data collection was carried out with the school managers (directors, coordinators and teachers) through questionnaires on travel behavior of the students, information on any activities at school to teach students to behave in traffic, opinions of management on the relationship between physical activity and health of children, children's ability to to walk to school, the barriers surrounding the school and attitude of managers regarding the use of different modes of transport and the impact of vehicle traffic in urban . The results showed that the estimation of directors and coordinators on the modes of transport used by students does not differ from a survey conducted by teachers significantly, and in private schools motorized individual transport is the most used by students and public schools over half of students walk or use public transportation to school. It was found that the traffic behavior on teaching is applied in all schools, although the directors and coordinators are more likely to say that the activities are carried out in your school. The school managers have a positive perception of the importance of physical activity, with acceptance rates above 90%. There is no consensus about the best age and abilities of children to make safe decisions alone and analysis on the perception of managers of barriers around the schools that may hinder access to foot it was found that about half of the managers have positive about the quality of the environment around the schools, but other managers have indifferent opinion about the quality of the environment. Regarding the use of different modes of transport it was observed that attitudes regarding the use of buses are in general negative, more than half of managers are indifferent to automobile use and favorable to the use of the walk. In the manager's attitude regarding the impact of vehicle traffic in urban areas the vast majority of managers are concerned about the impact of traffic on the environment. Perceptions regarding the active mobility should be worked through school policies to improve students' skills as pedestrians and through public policies to improve the environment in the school environment and encourage the use of active modes of transportation. / As cidades brasileiras nas ultimas décadas vem apresentando um predomínio do uso do automóvel como modo de transporte das crianças para a escola. Ao realizarem o percurso casa-escola por modos motorizados as crianças praticam menos atividades físicas com prejuízo para a saúde. Políticas desenvolvidas nas escolas podem desempenhar um papel importante na determinação do modo como os estudantes viajam para a escola. Neste contexto, esta pesquisa visa identificar quais os fatores que influenciam na percepção dos gestores sobre a utilização da caminhada para acesso a escola. O foco da pesquisa são as escolas municipais e particulares de ensino fundamental 1. O estudo de caso foi realizado na cidade de São José do Rio Preto. A coleta de dados foi realizada junto aos gestores escolares (diretores, coordenadores e professores) através de questionários sobre comportamento de viagem dos alunos da escola, informações sobre eventuais atividades desenvolvidas na escola para ensinar aos alunos com se comportar no trânsito, opiniões dos gestores sobre o relacionamento entre atividades físicas e saúde das crianças, a habilidade das crianças para caminharem até a escola, as barreiras existentes no entorno da escola e atitude dos gestores com relação à utilização dos diferentes modos de transporte e ao impacto do tráfego de veículos no meio urbano. Os resultados mostraram que a estimativa dos diretores e coordenadores sobre os modos de transporte utilizados pelos alunos não difere significativamente do levantamento realizado pelos professores, sendo que nas escolas privadas o transporte individual motorizado é o mais utilizado pelos alunos e nas escolas públicas mais da metade dos alunos caminham ou utilizam o transporte coletivo para a escola. Verificou-se que o ensino sobre comportamento no trânsito é aplicado em todas as escolas, embora os diretores e coordenadores sejam mais propensos a afirmar que as atividades são realizadas em sua escola. Os gestores escolares têm percepção positiva sobre a importância da atividade física, com taxas de aceitação acima de 90%. Não há um consenso sobre a melhor idade e as habilidades das crianças para tomar decisões seguras sozinhas e nas análises sobre a percepção dos gestores das barreiras existentes no entorno das escolas que podem dificultar o acesso a pé constatou-se que cerca da metade dos gestores tem opinião positiva sobre a qualidade do ambiente no entorno das escolas, porém os demais gestores possuem opinião indiferente quanto a qualidade do ambiente. Com relação à utilização dos diferentes modos de transporte observou-se que as atitudes com relação ao uso do ônibus são em geral negativas, mais da metade dos gestores são indiferentes ao uso do automóvel e são favoráveis às caminhada. Na atitude do gestor com relação ao impacto do tráfego de veículos no meio urbano a grande maioria dos gestores é preocupada com o impacto do tráfego no meio ambiente. As percepções com relação à mobilidade ativa devem ser trabalhadas através de políticas escolares para melhorar as habilidades dos alunos quanto pedestres e através de políticas públicas para melhorar o ambiente no entorno escolar e incentivar o uso dos modos ativos de transporte.

Grande parte da comunidade internacional defende que as cidades devem ser sustentáveis, inclusivas, seguras e resilientes. Neste sentido, tem sido desenvolvidas diversas iniciativas que, normalmente, envolvem revitalização de áreas urbanas, implementação de infraestrutura e criação de políticas de incentivo ao transporte não motorizado, como o deslocamento a pé. Apesar das evidentes vantagens deste tipo de transporte, como o baixo impacto ambiental e a prática de exercícios físicos, a vulnerabilidade dos pedestres é maior devido a sua exposição direta à fatores externos. Essa realidade se agrava quando são consideradas regiões de trânsito no entorno de escolas, colégios ou outras instituições educacionais, àreas estratégicas por atrairem novos usuários para a rede local de tráfego, incluindo as crianças. Esta dissertação trata sobre projeto de um aplicativo para promover o deslocamento de pedestres em áreas escolares, estimulando o transporte ativo entre crianças e adolescentes. A metodologia de projeto utilizada para a criação do sistema teve como base teórica e prática o design de interação, e considerou paralelamente as pesquisas específicas sobre o deslocamento de pedestres em áreas escolares obtidas na revisão teórica. A usabilidade e o potencial do sistema para o incentivo ao transporte ativo foram validados através de testes com um protótipo semi-funcional e de discussões orientadas em grupo focado. Os resultados obtidos nos testes foram majoritariamente favoráveis à adesão ao uso do aplicativo e ao seu potencial para incentivo à realização de deslocamentos a pé no trajeto casa/escola, de acordo com diversos critérios de avaliação fundamentados na revisão teórica. / A large part of the international community argues that cities must be sustainable, inclusive, secure and resilient. In this sense, several initiatives have been developed that involve revitalization of urban areas, implementation of infrastructure and creation of policies to encourage non-motorized transportation, such as walking. Despite the obvious advantages of this type of transport, such as low environmental impact and physical exercise, the vulnerability of pedestrians is greater due to their direct exposure to external factors. This reality worsens when they are considered transit regions around schools, colleges or other educational institutions, strategic areas for attracting new users to the local traffic network, including children. This thesis deals with the design of a mobile application to promote the movement of pedestrians in school areas to stimulating active transportation among children and adolescents. The project methodology used for the creation of the system was based on interaction design, and considered the specific research on the pedestrian movement in school areas carried out in the theoretical revision. The results obtained in the tests were mostly positive according to several evaluation criteria based on the theoretical review, indicating the adherence to the use of the proposed mobile app and showing its potential as an incentive children and teenagers walking.

Aim: The aim of this degree project is to investigate the current condition of sustainable infrastructure in Sundbyberg City and how the city can encourage and increase active transport. Method: The methods used are interviews with politicians and a transport expert within the city of Sundbyberg as primary data collection. The other methods are secondary survey data provided by Sundbyberg City that provides the transport modes of the participants of the city in late 2022 and the third method is a site visit to the city center where many different modes of transport interact daily. Results: The findings indicate that the city is in need of improving the infrastructure for active transport. The usage of cars increased in the city since 2019 and this could possibly be due to the coronavirus pandemic that forced people to isolate and take their cars. Gender plays also a role in the choice of transport as more women tend to drive in Sundbyberg and walk less. Studies indicate a safer and better active infrastructure could encourage women to turn to active transport. The positive findings are that the interviews have shed light on new setts of policies that will help reduce the usage of cars and increase active transport. In particular, during the summer of 2023, two famous streets will be closed to cars and only walking and cycling will be allowed. Furthermore, many parking spaces will be turned into parks to reduce the usage of cars and encourage people to turn to more sustainable modes of transport. Conclusion: Sundbyberg city needs to improve the infrastructure for active transport by building wider lanes, separated from cars and if possible colored to highlight it for the users because that could have a positive impact on the residents and most likely encourage many to start walking and cycling. The importance of increase in active transport is improvement in the health of the public while also reduce the usage of cars. The politician and the transport experts in the city seem to be aware of the importance of better infrastructure for active transport and the importance of reducing the usage of cars as that will reduce emissions and air pollution. However, the cost seems to be a crucial factor that stands in the way of many projects. On the other hand, new policies such as closing streets from car traffic, removing parking spaces with green areas, and encouraging people to cycle even during the winter by providing salted and maintained lanes might prove to be equally important to better infrastructure and increase the city’s goal of becoming a more sustainable transport city.

Participation rates in Australia remain low compared to other international settings, and gross inequities exist in participation, including for women and people living in low socioeconomic areas [1]. In recognition of the health and environmental benefits of increasing cycling participation, governments in Australia are increasing investment in initiatives to increase bicycling [2]. Recent research found that 78% of people in Greater Melbourne (a major Australian metropolitan region) are interested in riding a bike [3]. This demonstrates an opportunity for considerable modal shift to bike riding. There is, however, insufficient knowledge of the barriers to, and enablers of cycling for transport in this context. Research of barriers and enablers in Australia is very limited, however the majority of what has been conducted bas been quantitative research in groups whom are already cyclists. Existing research explores factors affecting cycling for all purposes, potentially overlooking differences in barriers and enablers reported for either riding a bike for transport, or recreational purposes. Further, quantitative studies often present the prevalence of a particular barrier or enabler, without consideration of the strength of how preventative, or encouraging the factor may be. This has resulted in cycling strategies being largely uninformed by the needs of people who are not current cyclists, and without consideration of the needs of people who ride a bike for transport, compared to recreational ri.ders. To increase cycling participation, it is essential to understand the barriers and enablers of cycling for all people of all ages and abilities, and to understand the nuances of their perception of safety. This requires a mixed-methods approach, with a robust sampling approach, to consider the prevalence and strength of the varying factors that influence people's decision to ride a bike or not. We conducted an online survey and semi-structured interviews with people living in nine selected local government areas across Greater Melbourne. [From: Introduction]

O transporte de α-metil glicosídio ( α-MG) em Saccharomyces cerevisiae foi recentemente reportado como transporte ativo, do tipo simporte de &$945;-MG com H+ mediado pela permease Agt1p. A cepa AP77-11B (cepa selecionada em nosso laboratório) 14C-α-MG pelo mecanismo descrito como difusão facilitada porque não existe co-transporte de H+ durante o transporte de α-MG. Os genes HXT1-HXT17 pertencem à família dos transportadores de hexoses em Saccharomyces cerevisiae. Então, nós decidimos investigar a possibilidade que o transporte de α-MG poderia ser mediado pelos transportadores de hexoses. Nós demonstramos que cepa MC966A (tipo selvagem), KY73 (isogênica com MC966A mas deletada nos HXT1-7), BSY08 (isogênica com KY73 com o AGT1 deletado), BSY09 (isogênica com MC966A com o AGT1 deletado) e a EBY.VW4000 (hxt1-17 agt1 gal2-null), não cresceram em α-MG como fonte de carbono. Além disso, estas cepas não transportaram α-MG por difusão facilitada quando as células foram cultivadas em meio com maltose, levando-nos a concluir que os transportadores de hexoses não estavam envolvidos no transporte de α-MG. Nós observamos que a cepa AP77-11B apresentou alta atividade de α-metilglicosidase periplásmica quando as células foram cultivadas em α-MG. Esta atividade enzimática foi ensaiada usando um método descrito primeiramente para invertase periplásmica, no qual as células eram incubadas com fluoreto de sódio, um inibidor da enolase, antes da incubação com α-MG. Então, a glicose produzida durante a hidrólise do -MG poderia ser determinada. A atividade extracelular só está presente em células cultivadas em -αMG. Células de-reprimidas não mostraram atividade de alpha-metilglicosidase. Os parâmetros cinéticos determinados para α-metilglicosidase, indicaram que esta enzima tem baixa afinidade para o alpha-MG. Além do mais, a atividade específica da alpha-metilglicosidase periplásmica aumentou ao longo da curva de crescimento em α-MG. Os resultados reportados mostraram que existem duas vias de utilização de α-MG em Saccharomyces cerevisiae. Uma via é mediada pela Agt1p, responsável pelo transporte ativo de α-MG. Na outra via, a α -metilglicosidase é secretada para o espaço periplásmico das células. Então, a glicose produzida pela hidrólise do α-MG é transportada pelos transportadores de hexoses por difusão facilitada. / Alpha-Methyl glucoside ( alpha-MG) transport in Saccharomyces cerevisiae was previously reported to be an active transport, a H+ -symport mediated by the Agt1p permease. Strain AP77-11B (a strain obtained in our laboratory) takes up 14C- alpha-MG by a mechanism which was ascribed to be facilitated diffusion since there is no H+-cotransport during the alpha-MG uptake. The HXT1-HXT17 there is no H genes belong to a family of hexose transporters in Saccharomyces cerevisiae. Therefore, we decided to investigate the possibility that -MG transport could be mediated by hexose transporters. We demonstrated that strains MC966A (w.t.), KY73 (isogenic to MC966A but hxt1-hxt7-null), BSY08 (isogenic to KY73 with AGT1 deleted), BSY09 (isogenic to MC966A with AGT1 deleted) and even strain EBY.VW4000 (hxt1-hxt17 agt1 gal2-null), were not able to grow on alpha-MG as the sole carbon source. Moreover, none of them presented alpha-MG transport by facilitated diffusion when the strains were grown on maltose leading us to conclude that the HXT glucose transporters were not involved in alpha-MG transport. We found that strain AP77-11B displayed a high periplasmic alpha-methylglucosidase activity when cells were grown on alpha-MG. This enzymatic activity was assayed using a method first described for periplasmic invertase in which cells were incubated with sodium fluoride, an inhibitor of enolase, prior to the incubation with alpha-MG. Then the glucose produced during alpha-MG hydrolysis could be accurately measured. The extracellular activity was present only in cells grown on alpha-MG. Glucose derepressed cells did not show periplasmic alpha-methylglucosidase activity.