Awareness systems are being designed and implemented to improve employee connections. This study examines the variables that affect the acceptance of an awareness system. The awareness system that was used for this research was a remote desktop access system. The independent variables investigated were the degree of detail that can be viewed on a desktop, whether the users can control who can access their desktops, whether the users can control when others have access to their desktops, the equality of access to others' desktops, and task-technology fit. In determining the effect of the independent variables on acceptance, the dependent variable, the mediating variables of privacy and fairness were taken into account. There was a preliminary survey conducted to determine appropriate situations to be used in the scenario descriptions for the survey for the main study. The methodology of policy-capturing surveys was utilized to conduct the survey for the main study in order to investigate the model developed in this study. The policy-capturing survey was pre-tested on University of Waterloo students. The main study was conducted in two different organizations, the subjects for the first study were employees from the Information Systems and Technology Department at the University of Waterloo and the subjects for the second study were employees from Ciber Incorporated. Results indicate that perceptions of privacy and perceptions of fairness have significant effects on acceptance. Also, perceptions of privacy and fairness are related to details in the design of the remote desktop access system. This research may be a contribution to this field since little research has been conducted in this area and implications can be drawn for future research on acceptance of awareness systems.

The current environment for drug discovery and disease treatment relies heavily on genomic analysis, structural biology and chemical biology techniques. With the enormous advances in genomic analysis and structural biology, the use of and desire for targeted therapies has increased. However, as more genomic data for cancer disease state pathology becomes available we must ask increasingly difficult questions and even produce new technologies, such as activity-based probes, to answer these questions. In particular, targeted kinase inhibitors for the treatment of cancer has become a mainstay for drug development for both industry and academia, but it is evident that the genomic data is not always indicative of protein expression. Additionally, protein expression alone does not completely characterize functional activity. Therefore, in order to more accurately validate drug targets and predict drug efficacy, we must not only identify possible targets but also determine their activity in vivo. The goal of this work was to develop a probe for Protein Kinase A that would act by alkylating a conserved cysteine in the substrate-binding pocket of the enzyme. We hypothesized that by targeting the substrate-binding pocket we could effectively utilize the natural substrate selectivity filters as well as take into account multiple endogenous regulatory mechanisms. We produced probes utilizing portions of the pseudosubstrate inhibitor PKI that demonstrate the ability to label the catalytic subunit of Protein Kinase A in an activity-dependent manner, thus making it an important first step in a new class of activity-based probes for the kinome.

Neighbourhood design is a part of sustainable development, and attempts to create better places for people by reflecting residents' values, as well as coordinating powers, resources, and actions between stakeholders. This research aims to investigate neighbourhood regeneration plans using an activity-based approach. It explores how daily social activities can be modelled using the case study of Brunswick, to develop the analytical and decision-support roles of modelling in the urban design process. The literature about the role of design in urban development argues that the designers' attempts to predict how new environments will actually be used have not often been successful. It is assumed that if the design process incorporates human-centred activities in creating and maintaining neighbourhood places, the certainty and reliability of any future vision will be enhanced. Following a critical review of the concept of activity in urban design and urban modelling, the daily activities of Brunswick residents are investigated through the residents' completion of daily diaries. This data contributes to an analytical activity-based approach. The outcomes provide the analyses of the following areas: the activity choices of individuals and how these choices are linked with the socio-demographics of residents, how current local agencies and the existing physical environment provide opportunities and restrict residents' participation in local activities, and the future activity-based scenarios of change as a result of the neighbourhood regeneration proposal submitted to the Manchester City Council. Ultimately, an activity-based framework is proposed which enables the qualitative analysis of regeneration planning statements, community consultation reports, institutional programmes, and policy documents. The framework then outlines the method of integrating the three above mentioned quantitative results with the qualitative data, and the formation of activity scenarios. This thesis demonstrates how this framework assists local agencies, authorities, service providers, and regeneration teams with regard to improving their services, promoting social integration of residents in neighbourhoods, and linking neighbourhoods to their wider contexts. The modelling results reveal that the elderly residents of Brunswick are isolated, the Brunswick spaces are not frequently used in the evenings, and the existing activities are not popular for male, employed and highly qualified residents. Although Brunswick Church attempts to be the community centre of the Brunswick neighbourhood, the provision of activities in this place is via other organisations. Medlock School and the Sure Start Children's Centre are the community hubs for parents and children to socially interact with other members. In addition, the new layout of the Brunswick neighbourhood after the regeneration scheme will improve the safety level of the Brunswick spaces, but will partially link Brunswick to Manchester. There is the lack of exchanging data as well as the absence of the indication of the reasons and processes that have contributed to the current challenges in Brunswick. Hence, there is inconsistency between the stakeholders' views towards the neighbourhood issues, and limited evidence is available for stakeholders to have a robust future outlook to the regeneration plan.

The ubiquitylation cascade regulates multiple cellular functions and is involved in numerous diseases. The distinct transfer cascade, involving E1-E2-E3 enzymes, serves as a promising target for drug development. However, E3 ligases (E3s) represent an important class of enzymes yet there are currently no effective tools for profiling their activity. Herein, a new class of E3 activity-based probe (ABP) is presented which is built by re-engineering ubiquitin (Ub)-charged E2 conjugating enzymes. The utility of these probes has been demonstrated by the rapid dissection of the activation determinants of the RING-Between-RING E3 (RBR) E3, Parkin. Furthermore, biotin-E3 ABPs allow us to systematically discover and dissect the E3 activities of a broad spectrum of E3s that are associated with different diseases. By interfacing the ABPs with mass spectrometry, we establish an activity based protein profiling (ABPP) system and apply it to uncover a new class of E3. We show that MYCBP2 is an E3 ligase with a novel mechanism of action that ubiquitylates threonine residues. MYCBP2 contains a RING domain, that recruits the ubiquitin-loaded E2, and a novel Zn-binding fold that contains two catalytic cysteine residues which relay the Ub to substrate via two thioester intermediates (RING-Cys-Relay, RCR). This discovery demonstrates the power and potential of our E3 activity based protein profiling (ABPP) system.

半導體產業在近二十年來一直在我國經濟發展舞台上扮演積極重要的角色，其績效自然是研究者欲探索之重點。在管理會計頗有作用的作業制成本制度，其提供不同於以往傳統成本制度之資訊，在在替全球知名企業創造更具優勢的管理績效。本研究之重心即在：以深入訪談及田野實證研究方式探討半導體公司導入作業制成本制度後，所發生的績效變化、變化時間與相關資訊，用實證資料檢視作業制成本制度與公司實際經營績效之間的關係，為作業制成本制度在企業之成效作一較為完整且具實務運用的描述，進而提供後續推行作業制成本制度之研究價值，並給予實務界導入作業制成本制度之相關資訊，以作為我國企業推動作業制成本管理制度之參考。 　　本研究以田野實證之資料為主，並以迴歸方式驗證作業制成本資訊釋出前後對企業生產成本與品質之影響，實證結果顯示成本會隨資訊釋出而降低，品質則不會有所變化，表示作業制成本制度確實可以幫助管理者從事成本抑減之工作；也得到品質不會在短期內改善之結論，暗示作業制成本制度之財務績效先於品質績效，資訊使用者之熟練程度差異可能影響績效之出現與否。 　　基於研究所得結論，本研究建議個案公司可以將成本下降之經驗擴散至全廠區，藉由新制度教育員工成本與獲利觀念，並針對員工之使用感想修正個案公司之作業制成本制度。同時也建議未來的研究者，對品質與作業制成本制度甚至成本之關係，作更進一步之研究。 / Using field study method, this study focuses on the relationship between the manufacturing performance of the semi-conductor industry and activity-based costing system information. The analysis suggests that with better decision based on activity and cost driver information, the actvity-based costing information will reduce manufacturing costs while leave quality unchanged. 　　In conclusion, empirical results reveal that manufacturing costs actually decrease with the release of activity-based costing information, while manufacturing quality remains the same. The finding shows that the release of activity-based costing information can reduce the manufacturing costs, but there may not be an immediate effect of quality improvement, suggesting that cost can actually be improved by the activity-based costing system, and quality may need more time and skill toward activity-based costing to change.

El objetivo principal de esta tesis ha consistido en Desarrollar una propuesta de implantación del ABC al sector hotelero. También se ha perseguido revisar los sistemas de costes aplicados al sector hotelero para superar los inconvenientes que presentan, mejorar la comprensión del ABC orientado a las empresas de servicios y exponer una revisión de las últimas tendencias en la investigación sobre el ABC.La estructura de esta tesis es la siguiente. Tras una breve introducción, se estudian en el primer capítulo la evolución y las fuentes doctrinales del ABC. El segundo capítulo se dedica al análisis de la viabilidad del sistema ABC en empresas de servicios. Se han revisado distintas publicaciones al respecto sobre casos tan diversos como los referidos a ferrocarriles, hospitales, bancos, empresas financieras, comunicaciones, compañías de seguros, etc.En el tercer capítulo se estudia el sector turístico y las particularidades de la empresa hotelera, revisando las publicaciones de estudios de costes más destacados relacionados con la misma y de estudios de ABC en hoteles. En los capítulos cuarto y quinto se han desarrollado la aplicación práctica del sistema ABC en una empresa hotelera real mediante el método del caso. Primero se exponen las fases de la implantación llevadas a cabo en relación con la identificación, codificación y análisis de las actividades realizadas en los distintos procesos de la empresa. Se han elaborado los mapas y el diccionario de las actividades. Se ha realizado la fase de racionalización de las mismas con el fin de agregarlas o desglosarlas según convenga. Después se han analizado todos los elementos de coste consumidos en la empresa, determinando después el coste de las actividades y de los servicios y productos. También se calculan los resultados obtenidos en la organización. Las conclusiones alcanzadas están relacionadas con el sistema ABC en general y con la aplicación del mismo al sector hotelero. Entre ellas remarcamos el hecho de haber comprobado que el ABC es aplicable a empresas de servicios y, en especial a las hoteleras, al menos en el caso estudiado y de haber constatado que es crucial tener en cuenta el factor humano (trabajadores, dirección, equipo del proyecto) en el proceso de aplicación del ABC. / The primary target of this thesis has consisted of Developing a proposal of implantation of the ABC to the hotel sector. Also it has been persecuted to review the systems of costs applied to the hotel sector to surpass the disadvantages that present/display, of improving the understanding of the ABC oriented to the companies of services and to expose a revision of the last tendencies in the investigation on the ABC. The structure of this thesis is the following one. After a brief introduction, the evolution and the doctrinales sources of the ABC have been studied in the first chapter. The second chapter is dedicated to the analysis of the viability of the ABC system in companies of services. Different publications have been reviewed on the matter on as diverse cases as the railroad referred ones, hospitals, banks, financial companies, communications, insurance agencies, etc. In the third chapter, the tourist sector and the particularitities of the hotel companies have been studied, reviewing publications of studies of more outstanding costs related to the same one and of ABC studies in hotels.In the chapters fourth and fifth the practical application of the ABC system in real a hotel company has been developed by means of the case method. First the carried out phases of the implantation in relation to the identification, codification and analysis of the made activities in the different processes from the company are exposed. The maps and the dictionary of the activities have been elaborated. The phase of their rationalization has been made. Later all the elements of cost consumed in the company have been analyzed, determining after this the cost of the activities and the services and products. Also the results obtained in the organization have been calculated. The reached conclusions are related to the ABC system in general and its application to the hotel sector. Among them we emphasizes the fact of to have verified that the ABC is applicable to companies of services and, in special to the hotel case studied and to have stated at least that is crucial to consider the human factor (workers, direction, equipment of the project) in the process of application of the ABC.

Awareness systems are being designed and implemented to improve employee connections. This study examines the variables that affect the acceptance of an awareness system. The awareness system that was used for this research was a remote desktop access system. The independent variables investigated were the degree of detail that can be viewed on a desktop, whether the users can control who can access their desktops, whether the users can control when others have access to their desktops, the equality of access to others' desktops, and task-technology fit. In determining the effect of the independent variables on acceptance, the dependent variable, the mediating variables of privacy and fairness were taken into account. There was a preliminary survey conducted to determine appropriate situations to be used in the scenario descriptions for the survey for the main study. The methodology of policy-capturing surveys was utilized to conduct the survey for the main study in order to investigate the model developed in this study. The policy-capturing survey was pre-tested on University of Waterloo students. The main study was conducted in two different organizations, the subjects for the first study were employees from the Information Systems and Technology Department at the University of Waterloo and the subjects for the second study were employees from Ciber Incorporated. Results indicate that perceptions of privacy and perceptions of fairness have significant effects on acceptance. Also, perceptions of privacy and fairness are related to details in the design of the remote desktop access system. This research may be a contribution to this field since little research has been conducted in this area and implications can be drawn for future research on acceptance of awareness systems.

No description available.

To face hyper-competitive environment, effective control on cost as a niche is the most important issue to business. However, properly allocation and compute accuracy on cost in the process of valid control is a major prerequisite. Therefore, how to decline variation on cost allocation through straight apportion which plays a key role on fully adequately cost. The Activity-Based Costing system¡]ABC system¡^ provides the major function properly for cost principle. The ABC system is not only making cost information efficiently but also assist Chief Executive Officer¡]CEO¡^ to make decision adequately under vary from minute to minute environment. This essay is focus on heavy machinery company as a subject through operation process analysis and responsibility center as a performance evaluation basis try to solve the currently problem that existed at responsibility center. Moreover, to seek out a proper costing amendments and allocation method for the case company after study that can enhance information quality for management on decision-making. Non-direct cost allocation to the case company declines during Activity-Based Costing System as a main shaft to adjust cost collective method. In the meanwhile, advance the costing accuracy makes management to get legitimate information is the most significant contribution to subject company.

In 2002 the Legislative Tuan promulgated the ¡§Charges Law.¡¨ Undoubtedly this has empowered county and city governments to levy charges/fees. Kaohsiung City Government has thus commissioned us to reexamine the reasonableness of the charges system of Kaohsiung city and study how to enhance the levy of charges, through investigating user charges/user fees, Activity Based Costing, and the charges system of counties and cities. This would increase the revenues of Kaohsiung city and comply with the Charges Law requiring local governments to periodically examine and adjust the regulations on charges. The study has found: (1) The fees charged by Kaohsiung City government agencies for city service can be increased. (2) Fewer than 30% of agencies of Kaohsiung City government as well as other county and city governments charge fees that are permitted by local ordinances and regulations. (3) Kaohsiung City has less revenues derived from levying charges than does Taipei City, primarily because the latter has more high rate, more people using public services and facilities and charges new fees. (4) As indicated by questionnaire survey, government agencies for citizen service of Kaohsiung City are not eager to adjust charges or levy new fees in the future. This study suggests Kaohsiung City to adjust charges and levy new fees in consideration of the distinctive conditions of city, by abiding by the ¡§equal, fair¡¨ principle, and by referring to other county and city governments in this regard.