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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Mikrobiologinių rodiklių kitimas smulkintoje mėsoje laikymo metu / Changes of microbiological parameters of minced meat during storage

Jagminaitė, Živilė 18 June 2014 (has links)
Darbo pavadinimas: Mikrobiologinių rodiklių kitimas smulkintoje mėsoje laikymo metu. Magistro baigiamojo darbo autorė Živilė Jagminaitė. Darbo vadovė dr. Aistė Kabašinskienė. Magistro darbas buvo atliktas Lietuvos sveikatos mokslų universitete, maisto saugos ir kokybės katedroje, per 2012-2014 laikotarpį. Darbas susideda iš: 51 puslapio, 2 lentelių, 29 paveikslų, 1 priedo. Atitinkamas mėsos laikymas bei apdorojimas, šviežumo užtikrinimas, higieninis – sanitarinis rėžimas yra svarbūs veiksniai užtikrinant mėsos mikrobinę kokybę. Žmonės dažnai nežino kaip laikyti pusgaminius, teisingai gaminti maistą, dėl ko atsiranda pavojus susirgti infekcinėmis ligomis. Darbo tikslas: įvertinti mikroorganizmų skaičiaus kitimą smulkintoje mėsoje, jos laikymo metu. Rezultatai: Aerobinių kolonijų skaičius smulkintos mėsos mėginiuose neviršijo Lietuvos Higienos normos HN 26:2006 nustatytų didžiausių leistinų ribų. Į smulkintą vištieną pridėjus prieskonių, aerobinių kolonijų sk. padidėjo 36,6 ksv/g, į smulkintą kiaulieną 1,82 ksv/g, o pridėjus kiaušinio ir džiūvėsėlių į vištieną padidėjo 8,5 ksv/g, į kiaulieną 19,09 ksv/g (p>0,05). Smulkintoje mėsoje su prieskoniais aerobinių kolonijų sk. po 3-6 val. padidėjo: vištienoje 7,4 ksv/g (p<0,05), kiaulienoje 205,32 ksv/g (p>0,05), o po 24 val.: vištienoje 169,1 ksv/g (p<0,05), kiaulienoje 466,22 ksv/g (p<0,05), o su kiaušiniu ir džiūvėsėliais aerobinių kolonijų sk. po 3-6 val. padidėjo: vištienoje 9,6 ksv/g (p>0,05), kiaulienoje 0,45 ksv/g (p<0,05)... [toliau žr. visą tekstą] / Title of the thesis: Changes of microbiological parameters of minced meat during storage. The author of this Master work is Živilė Jagminaitė. Work advisor: dr. Aistė Kabašinskienė. The Master work was completed in Lithuanian university of health sciences, Department of food safety and quality, during 2011-2012 period. Work consists of: 51 pages, 2 tables, 29 illustrations, 1 supplement. Appropriate storage and meat processing, freshness assurance and hygienic-sanitary regimes are important factors when ensuring meat microbial quality. In often cases people don‘t know how to hold semi-cooked food properly, how to correctly prepare food, resulting in the risk of developing infectious diseases. Thesis aim: to evaluate the change in population of microbes in minced meat during the period of its storage. Results: Aerobic colony count in samples of minced meat did not exceed those of Lithuanian Hygiene Standards HN 26:2006 maximum allowed limits. When added spices to minced chicken, aerobic colony count has increased by 36,6 CFU/g, to pork by 8,49 CFU/g, and once added egg and bread crumbs to minced chicken increased by 8,5 CFU/g, to pork by 12,1 CFU/g (p>0,05). In the holding period of 3 to 6 hours, the count of aerobic colony in minced chicken with spices has increased by 7,4 CFU/g, in pork by 205,32CFU/g (p>0,05) and after 24 hours in chicken reached by 169,1 CFU/g (p<0,05), in pork by 396,06 CFU/g (p>0,05). For egg and bread crumbs 3 to 6 hours has increased by 9,6 CFU/g... [to full text]
2

Practical use and development of biomérieux TEMPO® system in microbial food safety

Alsaadi, Yousef Saeed January 1900 (has links)
Doctor of Philosophy / Department of Food Science / Daniel Y.C. Fung / In the food industry, coliform testing is traditionally done by the time consuming and labor intensive plate count method or tube enumeration methods. The TEMPO® system (bioMérieux, Inc.) was developed to improve laboratory efficiency and to replace traditional methods. It uses a miniaturization of the Most Probable Number (MPN) method with 16 tubes with 3 dilutions in one single disposable card. It utilizes two stations: the TEMPO® Preparation station and the TEMPO® Reading station. In this study, the Oxyase® (Oxyase®, Inc.) enzyme was added to TEMPO® CC (Coliforms Count), TEMPO® AC (aerobic colony count) and TEMPO® EC (E. coli Count) methods. Water samples of 1 ml with 0.1 ml of Oxyase® enzyme were compared to samples without the Oxyase® enzyme using the TEMPO® system. Samples were spiked with different levels of coliforms (10, 102, 103 and 104 CFU/ml), stomached (20 sec), and pipetted into the three different TEMPO® media reagents (4 ml) in duplicate and then automatically transferred into the corresponding TEMPO® cards by the TEMPO® preparation station. Counts were obtained using the TEMPO® reading station after 8, 12, 16, 22 and 24 hours at an incubation temperature of 35°C. Results from 20 replicates were compared statistically. Using TEMPO® tests, high counts in food samples (>6 log 10 CFU/ml) can be read in 6±2 hours of incubation using the time-to-detection calibration curve. The TEMPO® system reduces reading time (reading protocol should be changed). There is no need to wait for 22 hours of incubation only 12 hours is required. Oxyrase® enzyme is not needed for the TEMPO® system.

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