Master of Science / Department of Agronomy / Michel D. Ransom / Many soils in southeastern Kansas are characterized by high clay contents and high shrink-swell potentials. Their vertic properties and claypan characteristics cause soil management to be difficult and pose problems for agricultural, environmental, and engineering uses. Thus, collecting more information and improving our understanding of these soils is an important step towards bettering our soil management techniques. The objectives of this study were to examine the morphology, processes of soil genesis, clay mineralogy, micromorphology, and potassium fixation potential of the soils of interest and how these characteristics varied between and within individual pedons. Ten pedons expected to represent varying degrees of vertic expression were selected. Methods included the use of field descriptions, routine soil laboratory characterization, micromorphological investigations, the determination of clay mineralogy by X-ray diffraction, and the measurement of potassium fixation potential. Field morphology reflected the geologic parent materials available in the region. The fine sediments that compose these clayey soils are primarily provided by the Pennsylvanian and Permian shales and limestones underlying this region and the Flint Hills to the west. Dominant pedogenic processes currently at work are clay illuviation and shrink-swell processes. Silty, non-expansive surface soils at all but sites 6 and 7 are thought to buffer the rapid wetting and drying cycles needed for maximum vertic expression. Four of the soils were dominated by smectitic minerals in the clay fraction while the rest exhibited a more mixed mineralogy. Disruption of illuvial clay features by shrink-swell movement was evident in thin section. Striated b-fabrics dominated the micromorphology except in non-expansive surface soils. K fixation of the soil surface was found to be negative in all soils, thus K fixation potential is considered very low. In subsurface horizons, K fixation generally increased with increasing vermiculite content. In addition to limited quantities of K-fixing clay minerals, naturally high K levels limited the amount of K fixation in this study. The information presented can be used to improve our understanding and management of high clay, vertic and claypan soils in southeastern Kansas.

Doctor of Philosophy / Department of Biology / Michael A. Herman / Determining the genetic mechanisms involved in organismal response to environmental change is essential for understanding the effects of anthropogenic disturbance. The composition of the bacterial-feeding nematode community is an excellent biological indicator of disturbance, particularly in grassland ecosystems. We have previously shown that grassland soil nematodes are responsive to perturbations in the field including the addition of nitrogen fertilizer. We are interested in how this perturbation affects the microbial community and downstream effects on the next trophic level, the bacterial-feeding nematodes. To determine the effects of disturbance on soil bacterial communities we used massively parallel sequencing and found that chronic nitrogen addition on tallgrass prairie significantly impacts overall bacterial community diversity and the abundance of specific bacterial taxa. Because native soil nematodes lack well developed genomic tools, we employed Caenorhabditis elegans as a model for native soil nematode taxa and used transcriptional profiling to identify 204 candidate genes regulated in response to altered bacterial diets isolated from grassland soils. To biologically validate our results we used mutations that inactivate 21 of the identified genes and showed that most contribute to fitness or lifespan in a given bacterial environment. Although these bacteria may not be natural C. elegans food sources, this study aimed to show how changes in food source, as can occur in environmental disturbance, has large effects on gene expression and those genes whose expression are affected, contribute to fitness. Furthermore, we identified new functions for genes of unknown function as well as previously well-characterized genes, demonstrating the utility of this approach to further describe C. elegans genome. We also investigated the function of previously well-characterized C. elegans defense pathways in our grassland soil bacterial environments and found that some are environment specific. Additionally, we found that cuticular collagen genes are important for lifespan, and appear to function downstream of known defense pathways. Overall, our results suggest that anthropogenic disturbance in grasslands alters the most basal components of the soil food web, bacteria and bacterial-feeding nematodes through the genes they possess and how they are expressed, and resultant bottom-up effects could have profound consequences on ecosystem health and function.

Master of Science / Department of Agronomy / Gary M. Pierzynski / Soil chemical and physical properties beneath cattle feedlot pens are largely unstudied. This project was conducted to survey select soil chemical and physical properties of soil beneath active open air cattle feedlots. At four cattle feedlots in Kansas, the concentrations of NH4-N, organic-N, organic-C, Cl-, and P were high at the surface and rapidly decreased within 1.00 m. At three of the four feedlots, NO3-N was generally below background concentration (4.1 mg kg-1) while one feedlot had a >75 mg kg-1 increase in the top 1.00 m. Based on feeding data, only a small percent (7.9 to 1.2) of the total N deposited on the surface was found in the top 1.00 m below the pen surface for a range of 25 to 60 years of operation. While in use, these feedlots do not appear to have a high potential for groundwater pollution from NO3-N leaching. However, if they were to become inactive they may pose a severe threat to groundwater quality from organic-N mineralization and NH4-N nitrification. If feedlots were closed and the land could be largely remediated by removing a layer of soil, these feedlots would have an average 48% profile N removed in a 0.25 m thick layer. A chamber, a modified vacuum desiccator, was tested for the investigation of NH3 volatilization from soil in the laboratory. Ammonia volatilization at the soil surface is dependent on air flow, soil and air temperatures, soil water content, pH, the concentrations of NH3 and NH4+ in the air and soil solution, and factors affecting soil temperature including humidity. This chamber was built to control and/or quantify as many of these variables as possible. A technique for quantifying and predicting NH3 volatilization is important because AFOs are one of the largest contributors of NH3 to the atmosphere, which can cause acid precipitation and particulate matter deposition downwind from the operation. The chambers created allowed for repeated measurements with little error and appear to be a feasible, inexpensive apparatus to investigate NH3 volatilization mechanisms. Using synthetic urine as an N source, NH3 volatilization was affected by initial soil moisture content and soil texture and may be affected by initial soil pH. This chamber has promise to provide excellent data to assist the efforts being made to understand and model NH3 volatilization from feedlot pens.

Doctor of Philosophy / Department of Horticulture, Forestry, and Recreation Resources / Kimberly A. Williams / The use of calcined clays contributes properties of nutrient and water retention to soilless root media, which varies greatly depending on the parent clay and calcining treatment. This research characterized phosphate (PO[subscript]4) sorption of various calcined clay products, including low volatile and regular volatile material (LVM and RVM) 2:1 Attasorb clays (Engelhard Corp.), 2:1 Terra Green LVM clays (Oil-Dri Co.), and Turface (Profile Products LLC) at various particle sizes; 1:1 kaolin clays (Thiele Kaolin Co.) in powder form, and diatomaceous earth (Diatomite, Eagle Picher Minerals, Inc.). Three of the calcined materials, Terra Green montmorillonite and Attasorb attapulgite (which had high PO[subscript]4-sorption based on isotherms), and diatomaceous earth (which had negligible PO[subscript]4-sorption) were evaluated as components of soilless root media in two separate greenhouse experiments. The effect of the calcined materials, rate of incorporation (0%, 5%, 10% and 20% by volume in a mix with peat and perlite), and PO[subscript]4-P application rate (0, 5, 15, 45 mg.L[superscript]-1 PO[subscript]4-P) on plant growth, effluent P content and water use were determined during production and post-production of Impatiens wallerana Hook f. 'Tempo Rose'. The calcined materials varied in their ability to adsorb PO[subscript]4-P and generally yielded L-type isotherms. Laboratory results indicated potential for substantive P retention by several of the calcined materials when used in container production. For most materials, PO[subscript]4-P sorption did not show pronounced pH dependence. During production and post-production, the test materials not only improved PO[subscript]4-P retention but also water retention and water use efficiency while still maintaining optimal physical properties at incorporation rates of 5 to 10%. Diatomaceous earth resulted in PO[subscript]4-P retention not significantly different from the calcined clays.

Master of Science / Department of Horticulture, Forestry, and Recreation Resources / Rhonda R. Janke / Petiole sap nitrate-nitrogen (NO3-N) analysis with handheld meters is a valuable tool in applying in-season nitrogen (N) for many crops. Sufficiency levels have been determined for several leafy green crops, including lettuce (Lactuca sativa L.) and cabbage (Brassica oleracea L.), but not for pac choi (Brassica rapa L.). The response of pac choi to different fertilizer rates and sources [conventional and organic] has established optimal soluble N application rates and Cardy meter sufficiency ranges. Greenhouse experiments were conducted during summer and fall of 2008 in Manhattan, KS. Conventional soluble fertilizer was formulated from inorganic salts with a 4 NO3-N: 1 ammonium ratio. Phosphorus was held at 1.72mM and K at 0.83mM for all treatment levels. The organic soluble fertilizer, fish hydrolyzate (2N:1.72P:0.83K), was diluted to provide the same N levels as with conventional treatments. Both fertilizers were applied at rates of 0, 32, 75, 150, 225, 300, and 450 mg. L-1. Seedlings were transplanted and fertilizer application began at 18 days. Plants were harvested at seven weeks (five weeks post transplanting) after receiving 15 fertilizer applications during production. Samples of the most recently matured leaves were harvested weekly and analyzed for petiole sap NO3-N and leaf blade total N concentration. Leaf count, leaf length, and chlorophyll content were also measured weekly. Fresh and dry weights were determined on whole shoots and roots. Optimum yield was achieved at the 150 mg. L-1 fertility rate with both conventional and organic fertilizers. Field and high tunnel experiments were conducted during fall 2008 to validate the sufficiency ranges obtained from the greenhouse studies. Based on field and high tunnel results, sufficiency levels of NO3-N for pac choi petiole sap during weeks 2 to 3 of production were 800-1500 mg. L-1, and then dropped to 600-1000 mg. L-1 during weeks 4 through harvest for both conventional and organic fertilizers sources. These ranges could vary based on the variety of the crop, the fertility of soil, and certain environmental factors such as photoperiod, light intensity. However, we found that petiole sap nitrate always increased to the point associated with the maximum biomass, followed by a plateau where sap nitrate remained constant. This characteristic of the Cardy meter can provide the growers with a practical methodology to generate their standard curves under specific conditions to guide in-season N applications. Total N in leaf tissue showed fewer fertilizer rate effects than petiole sap NO3-N. Chlorophyll content was not useful in evaluating pac choi N status.