The purpose of this research is to determine if anaerobic adhesive can be used as a prevailing torque locking feature. Maintaining preload in critical joints is the usual standard that anaerobic adhesives are held to in aerospace and other industry. To test if anaerobic adhesive can be used as a prevailing torque locking feature a test procedure was developed and implemented to measure the removal torque of threaded fasteners after an allotted cure time. In total, 191 threaded fasteners of different material and coatings were tested in the unseated and seated states with various strengths and varieties of anaerobic adhesive. A series of three tests were conducted: initial use, reuse with no added anaerobic adhesive, and a third test with added product to the bolt and nut to see how removal torque would behave in these conditions. It was found that using anaerobic adhesive as a prevailing torque locking feature is viable in many cases. No published work to date analyzes anaerobic adhesive at the standard of a prevailing torque locking feature.