The nutritional value of molassed sugar beet feed as a dietary component was assessed in a series of three experiments. In experiment 1, the effect of molassed sugar beet feed pellets (32 or 64 g/kg fresh weight silage) either ensiled or fed separately with big-bale silage on silage quality and performance of male castrate sheep (initial liveweight 29 kg) was studied. On average, the inclusion of molassed sugar beet feed increased silage dry matter, water soluble carbohydrate and lactic acid concentrations and decreased silage ammonia concentrations. Molassed sugar beet feed either ensiled or given as a supplement, significantly improved total dry matter intakes and liveweight gain (P< 0.05) and metabolisable energy intakes (P< 0.01) in lambs. Experiment 2 was carried out to investigate the effects of feeding two levels of fishmeal in addition to molassed sugar beet feed as supplements to big-bale grass silage fed ad libitum to beef steers of 290 kg initial liveweight. Bale silage was given alone or supplemented with 800 g molassed sugar beet feed/day, molassed sugar beet feed + 125 g fish meal/day or molassed sugar beet feed + 250 g fish meal/day. Supplementation of big-bale silage with molassed sugar beet feed did not increase total dry matter intake or improve liveweight gain. Adding FM to a diet composed of big-bale silage and molassed sugar beet feed significantly increased liveweight gain, total ruminal volatile fatty acid and ammonia concentrations. The effect of diets differing in the ratio of molassed sugar beet feed to barley and the inclusion of yeast culture on growth and rumen metabolism of sheep (initial liveweight 36 kg) was examined in experiment 3. Four diets, which were offered ad libitum , consisted of pelleted or molassed sugar beet feed, barley and soya-bean meal in the following proportions (DM basis) either A, 770:170:60 or B, 470:470:60. Diets A and B were fed with and without 2 g Yea-Sacc/head/day.

Thesis (doctoral)--Friedrichs-Universität Halle-Wittenberg, 1903. / Vita. Includes bibliographical references (p. 1-8).

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In Venezuela, four sheep fitted with rumen cannulas were fed the toxin-containing legumes <i>Leucaena leucocephala</i> (sun dried leaves and stems) and <i>Canavalia ensiformis</i> (grains). <i>L. leucocephala</i> did not have any apparent toxic effects on the animals, in spite of the presence of the toxic compounds 3,4 DHP and 2,3 DHP in the rumen. There were no significant effects on the rumen pH, concentrations of VFAs, microbial counts, rate of rice straw degradation or in the rumen outflow of liquids and solids. However, there was an increase in the concentration of ammonia in the rumen, and in the number of Gram negative rods isolated from the rumen of sheep when <i>L. leucocephala</i> was added to the diet. When <i>C. ensiformis</i> was supplemented the presence of canavanine in the rumen was confirmed. Sheep did not show signs of toxicity. There was no effect on rumen pH, microbial counts, degradation of rice straw or rates of outflow of liquids and solids from the rumen. There was a decrease in the rumen concentrations of valerate and ammonia and an increase in the numbers of Gram negative bacteria isolated from the rumen of sheep as the dietary inclusion of <i>Canavalia</i> increased. Several pure cultures capable of mimosine, 2,4 DHP and (in fewer cases) 3,4 DHP wre isolated. One of them, a Gram variable spore-forming rod, presumably a new species of <i>Clostridium</i>, was characterised. The presence of degraders of mimosine and DHP in the rumen presumably accounted for the lack of toxicity of <i>L. leucocephala</i> in Venezuela. The effect of canavanine on the growth and products of fermentation of pure cultures was independent of the Gram staining reaction and the results of experiments using media containing either peptides and amino acids or free amino acids alone suggest that canavanine may have an effect on peptide hydrolysis.

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The roam objectives of this PhD project were to relate anal~ te solubility in supercritical carbon dioxide via molecular structure and also to investigate the factors that influence the solubility and extraction of analytes in a supercritical fluid extraction (SFE) when using carbon dioxide as the solvent. The polarity of an analyte was selected as the key parameter to developing a means of estimating steroid solubility in supercritical carbon dioxide. Polarity can be estimated by the hydrophobicity term, log P (based on partition coefficients), and also of the solubility parameter, 0,. The use of partition coefficient in conjunction with a calculated solubility parameter was demonstrated as a reasonable means of estimating steroid solubility in supercritical carbon dioxide. Experimental determination of the solubility of several steroid compounds with a range in polarities in supercritical carbon dioxide was carried out in order to correlate solute polarity to the solute solubility. A chromatographic method was also investigated based on capacity factor measurements for the prediction of steroid solubility in supercritical carbon dioxide. The application of supercritical fluid extraction (SFE) \\<ith carbon dioxide and modified carbon dioxide for the extraction of four antifungals from an animal feed matrix has been investigated. The SFE experiments were designed to optimize e:\.1:raction conditions for the extraction of the antifungals from the animal feed to allow for the evaluation of the most significant variables influencing extraction. A method was also developed for the analysis of the SFE animal feed extracts by packed-column supercritical fluid chromatography. The modification of the mobile phase with polar modifier was necessary to elute the antifungals. The procedure provided an alternative separation selectivity to the existing reversed phase high performance liquid chromatography techniques with much shorter analysis time.

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