The role of vitamin K in the blood clotting cascade has been well documented. Vitamin K has recently been implicated in improving bone health. The current studies were conducted to determine the effects of vitamin K in diets with and without mycotoxin contaminated corn on growth performance, bone characteristics, and related blood metabolites in pigs from weaning to market. Menadione sodium bisulfite complex (MSBC, 33% vitamin K) was chosen as the source of supplemental vitamin K because it is the most common form fed to swine. Vitamin K was tested at 0, 0.5, and 2.0 ppm in a corn-soybean meal based diets on two generations of pigs to evaluate any time and dose responses. The first generation of pigs was subjected to mycotoxin contaminated corn in the nursery phase to test for any interactions between the toxins and vitamin K. The addition of 0.5 ppm vitamin K reduced (P < 0.0001) prothrombin time. No additional decrease in prothrombin time was detected when increasing vitamin K inclusion from 0.5 to 2.0 ppm. With regard to growth performance, daily gain, feed intake, and feed efficiency were unaffected (P > 0.10) by supplemental vitamin K. However, pigs fed mycotoxin contaminated corn ate less (P = 0.005) and grew slower (P = 0.015) compared to those receiving good corn. The addition of vitamin K did not alleviate the negative growth effects in response to corn type. Vitamin K did not affect bone characteristics (P > 0.10), blood Ca (P > 0.05) or OC (P > 0.10). Other than blood clotting it does not appear that dietary vitamin K provides any additional benefits at these levels of inclusion and stages of swine production.

Ten wether lambs were used to determine the effects of ergovaline consumption from endophyte infected tall fescue, on nutrient utilization and metabolism. Lambs were fed a diet of 23% endophyte free tall fescue seed (E-) and 77% concentrate from d -14 to -1 (adaptation phase). On d 0, five lambs were switched to an endophyte infected seed diet (E+) where they remained through d 14. Nutrient digestibilities tended to increase from adaptation through the acute (d 1 to 4) to subacute (d 10 to 14) phases when E- was fed. E+ digestibilities were highest (P < 0.05) in the acute phase. Lambs fed E+ had higher rectal temperatures in the acute (P < 0.01) and subacute phases (P < 0.05). Fecal recovery of ergovaline increased as day of collection increased in the acute and subacute phases. Lysergic acid fecal recovery increased with day of collection in the acute phase, but no effect was found in the subacute phase. Serum enzyme analyses did not indicate tissue damage from alkaloid consumption. These results demonstrate lambs try to adapt to endophyte infected fescue seed consumption through increased nutrient digestibilities and increased ergovaline and lysergic acid excretion.

Our objective was to know how the hepatic transcriptome expression of growing beef (Angus-cross) heifers (0.5 kg gain/day) was affected by the feeding of different sources of dietary (3 mg/day) Se supplements: inorganic Se (ISe, sodium selenite), organic (OSe, Sel-Plex®), or a blend (1.5 mg:1.5 mg) of ISe:OSe (Mix), compared to the adequate but non-Se supplemented “Control”. The biopsied hepatic tissues of these four groups heifers collected at day 168 (when liver Se assimilation had stabilized) after supplements of Se, was subjected to the microarray analysis to assess Se treatment effects. The results suggest that there were clear differences in the hepatic gene expression profile of the four Se treatment groups. 139 significantly treatment-induced differentially expressed transcripts were selected. Among them: 1) the gene expression profiles of Control and OSe appeared to be more similar than Control and ISe, 2) eight distinct gene expression patterns among treatments were identified and each of them indicates affected biofunctions and networks, 3) they were grouped as the expression profile relative to Control, there were solely and commonly affected transcripts for four Se treatments and they indicated different biofunctions, 4) of them, three microRNAs were identified and their predicated mRNA targets showed different biofunctions.

Two experiments were conducted to determine the effect of feeding diets with a 2009 and 2010 naturally-contaminated corn to weaning pigs. For both experiments three diets were blended to contain 100% 2010 naturally-contaminated corn (control), 50-50% blend of the 2009 naturally-contaminated corn and 2010 corn (Diet 2), and 100% 2009 naturally-contaminated corn (Diet 3). In Exp. 1, 24 crossbred pigs with an average body weight of 7.64 ± 0.70 kg were allotted to 4 replicates of 3 treatments with 2 pigs per pen, on the basis of gender, litter mate, and BW in a randomized complete block design. Fecal and urine samples were collected and dry matter, energy, and nitrogen apparent digestibility were determined. Dry matter, energy, and nitrogen digestibility were not affected by either Diet 3 or Diet 2 compared to the control diet. In Exp. 2, 30 crossbred pigs with an average body weight of 7.98 ± 1.15 kg were allotted to 3 replicates of 2 comparisons with 5 pigs per pen. Comparisons consisted of: 1) Control vs Diet 3, and 2) Control vs Diet 2. Two feeders were located in each pen containing one of the two diets. Feed preference and growth performance were determined. A preference for the feed containing 2010 corn feed was observed; pigs showed the ability to discriminate mycotoxin-contaminated feed (95.34 vs. 4.66%; P< 0.01). Nutrient digestibility was not affected by these diets, but a clear decrease in feed intake was observed in the pigs.

Feed intake is a key factor affecting pig performance; thus, the objective of these studies was to assess a variety of factors that could potentially affect intake in pigs in different production stages. Studies were conducted to determine the effects of flavor and diet complexity, Appetein™ (an alternative protein source), and graded levels of salt on swine feed intake and feed preference. Two newly developed flavors were used in nursery pig diets. The use of the two flavors did not increase feed intake (P > 0.05). Nursery pigs actually showed a preference for the control diet. Complex diet formulation does increase feed intake (P < 0.03) in nursery pigs when diets are not over-formulated. When flavor was added to lactation diets sow feed intake did not change compared to the control. The flavor did not affect litter performance (P > 0.05). When Appetein™ was added to lactation diets at 0.5%, pig weight and litter weight were numerically greater for the sows fed Appetein™ but not significantly so. Appetein™ did not affect feed intake. When nursery pigs were fed graded levels of salt (0.1, 0.5, and 0.8%) feed intake increased (P < 0.01) as salt level increased. Nursery pigs also preferred (P < 0.05) 0.8% salt over other levels the first two weeks after weaning when given a choice among diets.

The objective of these studies was to evaluate the effect of supplying mannan oligosaccharides (MOS) to pig diets on both weanling pig and sow performances. Nursery pigs challenged with LPS to stimulate an immune challenge had increased body temperature and respiratory rate as well as elevated serum cortisol and TNF-α concentration, MOS-supplemented pigs had a lower rectal temperature and respiratory rate which implied that MOS improves some aspect of the immune function of piglets. MOS supplementation in sow diets during late gestation and lactation had no effect on litter size, but the piglets from MOS-fed sows were heavier at birth (P = 0.04), at weaning (P = 0.03), and during the entire nursery period (P < 0.01). Moreover, milk fat and protein levels as well as the Ig concentrations in milk from MOS-fed sows were numerically higher (2 – 12%; P > 0.10) than control sows. Overall, MOS supplementation in the nursery diet may have limited effects on the growth performance, but may have some beneficial influence on pigs under immune challenge. Furthermore, including MOS to the sow diet during late gestation and lactation can potentially improve piglet body weight as well as growth during the suckling and nursery periods.

Equine pituitary pars intermedia dysfunction (PPID) typically occurs in horses older than 15 years of age and is characterized by hair coat abnormalities, muscle atrophy and decreased insulin sensitivity. The first objective of this research was to compare the rate of whole body protein metabolism and relative abundance of key factors in the signaling pathways associated with muscle protein synthesis and protein breakdown in response to feeding in Control and PPID horses. No differences (P > 0.05) were seen between the PPID and Control groups in whole-body protein metabolism or post-prandial activation of the muscle signaling pathways regulating skeletal muscle protein synthesis and breakdown. The second objective of this research was to determine if aged horses with PPID had reduced insulin sensitivity and alterations in the insulin-mediated signaling pathways in the skeletal muscle when compared to non-PPID, aged Control horses. Measures of insulin sensitivity and the activation of factors associated with protein synthesis and breakdown were similar between the PPID and Control groups (P > 0.05). Overall, insulin sensitivity and protein metabolism are similar between the PPID and Control groups. The studies suggest that abnormalities may exist as a function of advanced age rather than PPID status directly.

Determining digestibility of feed ingredients is a challenge. While forage type feeds may be fed as the sole dietary component, concentrates cannot. To determine the apparent digestibility of these ingredients a by difference method can be utilized. The study was conducted to investigate the nutritional value of corn germ dehydrated (AAFCO 48.32) for horses in comparison to two processed corns commonly fed to horses. The second objective was to compare total tract digestibility of the treatments to determine differences in digestibility of the various fiber fractions due to added concentrate and to use the difference method to determine nutrient digestibility of the three corn treatments. In addition, glycemic responses and fecal pH changes were used to assess and quantify the presence of associative effects in fiber digestibility and differences in site of digestion. A 4x4 Latin square digestibility trial was conducted using a starch intake level of 6 g/kg BW/day, offered in three equal meals. The control diet consisted of hay cubes, alfalfa pellets, corn bran and corn oil. The treatment diets contained the control diet plus one of the three processed corn treatments, cracked, steam flaked or corn germ dehydrated. Cracked corn was selected as a negative control and steam flaked corn as a positive control based on previous research showing differences in pre-cecal starch digestibility coefficients. The four diets were formulated to have equal starch, NDF and ADF components. Each experimental period was 21 days made up of 5 days adaptation, 11 days on feed and a 5 day total fecal collection. Indirect methods for estimating small intestinal starch digestion (glycemic response) and changes in the hindgut environment (fecal pH and acid concentrations) were used due to the use of non-surgically modified experimental animals. Mean total tract starch digestibility for all diets was high, control 92.2±4.9, cracked corn 96.6±1.0, steam flaked corn 99.2±0.4 and corn germ dehydrated 98.8±0.4 % (P>0.05). The process of steam flaking compared to cracking or dry corn milling resulted in a greater area under the blood glucose time curve in response to 1kg of corn treatment meal, suggesting increased pre-cecal starch availability. No statistical differences were observed on an equal starch basis between the three corn products (P>0.05). Cracked corn significantly lowered fecal pH compared to the control and corn germ dehydrated diets (P0.05) when comparing the control diet and the combined diets (control diet plus processed corn) and the three individual corn ingredients by the difference method. Even though mean digestibility of fiber fractions were not affected by diet, individual horse data suggests that negative impacts on fiber fraction digestion occurred. Surprisingly, the steam flaked diet did not appear to act as a positive control in limiting changes in fermentation kinetics when fed at this intake level. The process of steam flaking improves small intestinal starch availability but the reduction in particle size may increase the rate of microbial fermentation prompting the development of acidosis. The study findings suggest the corn germ dehydrated product to be a good feedstuff for horses requiring additional calories and high feed intakes. The DE value is comparable to steam flaked corn, without the negative impacts observed on fecal fermentation end products.

Three experiments were conducted to assess the efficacy of including selected feed additives in the diet of weaning and grow-finish pigs. Experiment 1 utilized 24 crossbred grow-finish pigs and measured the effect of added EHY on DM, N, and energy digestibility. There were no differences in DM, Energy, and N digestibility between diets 1 through 4. Experiment 2 utilized a total of 36 crossbred pigs [18 barrows, 18 gilts] in order to determine if preference would be shown when presented with naturally-contaminated corn. There were three dietary comparisons, Control vs Diet 2 (Comparison 1), Control vs Diet 4 (Comparison 2), and Diet 2 vs Diet 4 (Comparison 3). A preference was shown for the control diet over Diet 2, as well as for the control diet over Diet 4. Experiment 3 utilized a total of 24 crossbred pigs [12 barrows, 12 gilts] in order to measure the effect of contaminated corn on performance and DM, energy, and N digestibility. DM, energy, and N digestibility were affected by corn quality.

Endophyte-infected tall fescue produces many ergot alkaloids, which have been shown to be vasoconstrictive in various vessel types of bovine. On the other hand, substantial evidence has been reported on the vasodilative effects of formononetin and biochanin A in different vessel types in humans and rats. So, a study was conducted using mesenteric vasculature collected from heifers shortly after slaughter. After 2-h incubation with formononetin (F), biochanin A (B), or an ergovaline-containing tall fescue seed extract (EXT) and their combinations, vessels were mounted in a multi-myograph to determine their ergotamine-induced contractility. Results indicated that F and B at 1 × 10-6 M and their combination did not impact the contractile response to ergotamine in mesenteric vasculature. The pre-myograph incubation of mesenteric vasculature with EXT altered the contractile response manner to ergotamine. However, at higher concentration, F and B may alleviate the reduction of vasoconstriction caused by prior exposure to EXT. To our knowledge, this study was the first to investigate the interaction of ergot alkaloids and isoflavones on in vitro bovine mesenteric vasculature. However, further investigations are necessary to understand the mechanism behind the interaction of ergot alkaloids and isoflavones on vasoactivity.