Orientador: José Luiz Moraes Vasconcelos / Banca: Roberto Sartori Filho / Banca: Guilherme de Paula Nogueira / Resumo: O objetivo desse estudo foi avaliar o efeito das concentrações de progesterona no desenvolvimento folicular e a influência do diâmetro folicular e duração do proestro na concepção de novilhas púberes inseminadas após detecção de cio ou submetidas à IATF. No exp.1, 723 novilhas foram sincronizadas com o protocolo: D0: benzoato de estradiol (BE, 2,0 mg, Estrogin®) + CIDR®; D7: dinoprost trometamina (PGF2a, 12,5 mg, Lutalyse ). As novilhas foram distribuídas aleatoriamente no dia 0 para inserção de CIDR® sem utilização prévia (CIDR1) ou utilizado previamente por 18 dias (CIDR3) e retirada no dia 7 ou dia 9 seguido de detecção de cio e inseminação 12h após o cio. No exp.2, 1083 novilhas foram sincronizadas de acordo com o exp1, entretanto todos dispositivos foram removidos no dia 9 e os animais foram inseminados 48 h (0,5 mg, i.m., ECP® no dia 9), 54 ou 72 h (100 μg, im., Fertagyl® no dia da IATF). No exp.3 474 novilhas foram distribuídas aleatoriamente para receberem: D-1: 2mg de BE no grupo 3; D0: 1 e 2 mg de BE respectivamente nos grupos 1 e 2. Todas novilhas foram sincronizadas com CIDR1, receberam 12,5 mg de PGF2a no dia 7 e 0,5 mg de ECP no dia 9. No grupo 2 foi aplicado 200 UI de eCG no dia 9. As novilhas foram inseminadas 48 horas após a remoção do dispositivo, perfazendo assim 3 grupos experimentais: grupo 1 (1 mg de BE no D0), grupo 2 (200 UI de eCG no D9) e grupo 3 (CIDR por 10 dias). Nos exp.1 e 2 o diâmetro do maior folículo (ØFD) e amostras de sangue (P4) foram obtidas em um subgrupo de animais no dia 7 e dia 9. No dia da IA e IATF foi feita avaliação do ØFD em um subgrupo de animais no exp.3 e em todos animais no exp1 e 2. Amostras de sangue para dosagem de P4 foram colhidas 7 dias após a IA ou IATF em um subgrupo de animais do exp.1 e em todos animais nos... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The aim of this trial was to evaluate the effect of progesterone concentration in follicular development and influence of follicular diameter and length of proestrus in the conception rate of pos pubertal heifers inseminated after detection of estrus or submitted to TAI. In exp.1, 723 heifers were synchronized with the protocol: D0: estradiol benzoate (EB, 2.0 mg, Estrogin®) + CIDR®; D7: dinoprost trometamina (PGF2a, 12.5 mg, Lutalyse ). The heifers were randomly assigned on D0 for inserting CIDR® without prior use (CIDR1) or previously used for 18 days (CIDR3) and were withdraw on day 7 or day 9 followed by heat detection and insemination 12 hours after estrus. In exp.2, 1083 heifers were synchronized according to exp1, however all devices were removed in day 9 and the animals were inseminated 48h (0.5 mg, i.m., ECP® on day 9), 54 ou 72 h (100 μg, im., Fertagyl® on day TAI). In exp.3, 474 heifers were randomly assigned to receive: D-1: 2 mg of EB in group 3; D0: 1 and 2 mg of EB respectively in groups 1 and 2. All heifers were synchronized with CIDR1, received 12.5 mg of PGF2a on day 7 and 0.5 mg of ECP on day 9. In the group 2 was administered 200 UI of eCG on day 9. The heifers were inseminated 48 hours after device removal, so three experimental groups were formed: group 1 (1 mg of EB on D0), group 2 (200 UI of eCG on D9) and group 3 (CIDR for 10 days). In the exp.1 and 2 the diameter of the largest follicle (ØFD) and sample of blood (P4) were obtained of subset of animals on day 7 and day 9. On days of AI and TAI the diameter of the largest follicle (ØFD) was measured in the subset of animals in the exp.3 and all animals in the exp.1 and exp.2. Blood samples for P4 assays were harvested seven days after AI or TAI in subset of animals the exp.1 and all heifers the exp.2 and exp.3. Pregnancy diagnosis was... (Complete abstract click electronic access below) / Mestre

To identify factors important to the efficiency of artificial insemination in cattle, four studies were conducted. In the first study, the addition of cream to the inseminate was used in an attempt to increase accessory sperm number. On d 6 after insemination, 60 embryos were evaluated. The addition of cream to the inseminate had no effect on accessory sperm number. In the second study, cryopreserved semen of a marked bull (spermatozoa exhibiting a semi-flattened anterior head) was matched with semen from an unmarked bull (conventional sperm head shape) to determine competitively the effect of a deep uterine insemination on accessory sperm number. Forty embryos were recovered 6 d after insemination and the ratio of accessory sperm observed was different: 62:38 for unmarked semen in the uterine body and marked semen in the uterine horn, and 72:28 for unmarked semen in the uterine horn and marked semen in the uterine body (P < .05). In the third study, superovulated cows were utilized to determine the effect of artificial insemination time on fertilization status and accessory sperm number. Cows were inseminated once at 0 h (n=10), 12 h (n=10), or 24 h (n=10) after the first standing event. On d 6 after insemination, 529 embryos(ova) were recovered. Fertilization rates were 29% (0 h); 60% (12 h); and 81% (24 h)(P < .01). Percentages of embryos with accessory sperm were: 5 (0 h); 8 (12 h); and 41(24 h) (P < .01). In the fourth study, three experiments utilizing superovulated cows were conducted to provide a basis for distinguishing unfertilized ova from very early embryonic death. In Exp. 1, recovered d 6 unfertilized ova were classified morphologically as either: 1) typical, 2) satellite, or 3) fragmented. In Exp. 2, recovered d 6 unfertilized ova from the third study were classified morphologically, and typical ova were fixed. In Exp. 3, ultrastructural features of preovulatory, tubal-stage, and typical d 6 unfertilized ova were investigated. Preovulatory ova revealed normal ultrastructure; tubal-stage ova exhibited evidence of degeneration; typical d 6 ova were degenerated and contained no discernable organelles. The first three studies support the use of accessory sperm evaluation as an alternative measure of fertility. The final study provides a basis from which future embryologists may distinguish fertilization failure from very early embryonic death. / Ph. D.

No description available.

No description available.

Tesis por compendio / Los objetivos generales de esta tesis fueron desarrollar nuevos diluyentes de inseminación artificial (IA) suplementados con un análogo de GnRH y caracterizar el proteoma del semen de conejo. En el capítulo I, se evaluó la inclusión de un cóctel de inhibidores de proteasas en el diluyente de inseminación (DI) para evitar parte de la actividad proteasa del plasma seminal de conejo. La calidad seminal y la fertilidad no se vieron afectadas por el cóctel. Sin embargo, la prolificidad fue significativamente menor en el grupo experimental en comparación con los grupos de control positivo y negativo (8,2±0,22 vs. 9,3±0,23 y 9,2±0,26 gazapos por parto, respectivamente). De este capítulo, se puede concluir que la adición de una amplia variedad de inhibidores de proteasas en el diluyente de semen de conejo afecta negativamente la tasa de prolificidad y que en el futuro sería aconsejable probar inhibidores específicos de aminopeptidasas (AMIs). En el capítulo II, suplementamos el DI con AMIs (bestatina y EDTA), y estudiamos su efecto sobre la calidad seminal y el rendimiento reproductivo. La inclusión de AMIs no afectó la calidad seminal, ni la fertilidad (85,3 vs. 88,6%), ni la prolificidad (10,12 vs. 10,51 gazapos por parto) en comparación con el grupo control. Por lo tanto, concluimos que los AMIs se pueden utilizar en los DIs de conejo para inhibir parte de la actividad aminopeptidasa del plasma seminal (PS). En el capítulo III, probamos nuevos DIs de conejo que contenían AMIs con o sin nanopartículas de quitosano (CS)-sulfato de dextrano (DS) que atrapan el análogo de GnRH. Se estudiaron los siguientes diluyentes: C4 (4 µg de buserelina/coneja en medio control (MC): tris-ácido cítrico-glucosa suplementado con AMIs), C5 (5 µg de buserelina/coneja en MC), Q4 (4 µg de buserelina/coneja en nanopartículas CS-DS en MC) y grupo Q5 (5 µg de buserelina/coneja en nanopartículas CS-DS en MC). La fertilidad fue significativamente menor en el grupo C4 en comparación con los grupos C5, Q5 y Q4 (0,7 frente a 0,85, 0,85 y 0,82, respectivamente). Por el contrario, la prolificidad fue similar en los cuatro grupos experimentales. Por lo tanto, las nanopartículas de CS-DS como transportador de acetato de buserelina permiten reducir la concentración de la hormona en diluyentes con AMIs sin afectar la fertilidad ni la prolificidad. Por ello, la nanoencapsulación parece ser un sistema prometedor para proteger el análogo de GnRH en los diluyentes de IA de conejos. Por otro lado, el objetivo de los últimos tres capítulos fue caracterizar las proteínas del semen de conejo. En los capítulos IV y V, se estudiaron las proteínas del PS de conejo. Las muestras de semen se recuperaron utilizando 6 machos de cada línea genética (A y R) y seleccionando una muestra heteroespérmica del comienzo, del medio y del final de cada estación y de cada línea (24 muestras en total). En el capítulo IV, utilizamos la técnica de electroforesis en gel de poliacrilamida 1D. Siete bandas proteicas fueron significativamente diferentes entre las líneas genéticas y tres bandas entre las estaciones. En el capítulo V, se sometió el PS a nano LC-MS/MS y se identificaron y cuantificaron 402 proteínas. 23 proteínas se expresaron diferencialmente entre genotipos. Con respecto al efecto de la estación en el proteoma del PS de conejo, los resultados mostraron que no hubo un patrón claro de variación de proteína a lo largo del año. En el capítulo VI, se caracterizaron las proteínas del espermatozoide de conejo. Se recuperaron 6 muestras espermáticas utilizando 5 machos de cada genotipo y se sometieron a nano LC-MS/MS, identificándose y cuantificándose 487 proteínas. 40 proteínas se expresaron diferencialmente entre genotipos. En conclusión, los resultados de los tres últimos capítulos evidencian que el genotipo está relacionado con una abundancia específica de proteínas del PS y del espermatozoide. Finalmente, se creó la / The general objectives of this thesis were to develop new artificial insemination (AI) extenders supplemented with a GnRH analogue and to characterise the proteomic profile of rabbit semen. In chapter I, the inclusion of a protease inhibitors cocktail in the insemination extender (IE) to avoid part of the rabbit seminal plasma protease activity was evaluated. Seminal quality and fertility rate were not affected by the cocktail, having similar values between experimental and control groups. However, prolificacy rate was significantly lower in experimental group compared to positive and negative control groups (8.2 ±0.22 vs. 9.3 ±0.23 and 9.2 ±0.26 total born per litter, respectively). From this chapter, it may be concluded that the addition of a wide variety of protease inhibitors in the rabbit semen extender negatively affects prolificacy rate and it in the future it would be advisable to test specific aminopeptidase inhibitors (AMIs). Therefore, in chapter II, we supplemented the IE with AMIs (bestatin and EDTA), and we studied their effect on rabbit seminal quality and reproductive performance. Seminal quality was not affected by AMIs. Regarding reproductive performance, the inclusion of AMIs, did not affect fertility (85.3 vs. 88.6%), nor the prolificacy rate (10.12 vs. 10.51 kits per delivery) in comparison with control group. Thus, we concluded that AMIs can be used in rabbit IEs to inhibit part of the seminal plasma aminopeptidase activity. In chapter III, we test new rabbit IEs containing AMIs with or without chitosan (CS)-dextran sulfate (DS) nanoparticles entrapping the GnRH analogue. The following experimental extenders were studied: C4 (4 µg buserelin/doe in control medium (CM): Tris-citric acid-glucose supplemented with AMIs), C5 (5 µg of buserelin/doe in CM), Q4 (4 µg of buserelin/doe into CS-DS nanoparticles in CM) and Q5 group (5 µg of busereline/doe into CS-DS nanoparticles in CM). Results showed that fertility was significantly lower in C4 group compared to C5, Q5 and Q4 groups (0.7 vs. 0.85, 0.85 and 0.82, respectively). On the contrary, prolificacy was similar in the four experimental groups studied. Thus, CS-DS nanoparticles as carrier for buserelin acetate allow reducing the hormone's concentration in extenders supplemented with AMIs without affecting the fertility and prolificacy of rabbit females. Therefore, nanoencapsulation seems to be a promising system to protect the GnRH analogue in rabbit AI extenders. On the other hand, the aim of the last three chapters was to characterize rabbit semen proteins. In chapters IV and V, rabbit seminal plasma (SP) proteins were studied. Semen samples were recovered using 6 males from each genetic line (A and R). For each genotype, one pooled sample at the beginning, middle and end of each season was selected to develop the experiment (24 pools in total). In chapter IV, we used a 1D polyacrylamide gel electrophoresis approach. Seven protein bands were significantly different between genetic lines and three protein bands were significantly different between seasons. In chapter V, SP was subjected nano LC-MS/MS and 402 proteins were identified and quantified. Twenty-three proteins were differentially expressed between genotypes. Regarding the effect of season on rabbit SP proteome, results showed that there was no clear pattern of protein variation throughout the year. The results obtained in both chapters evidence that genotype is related to a specific abundance of SP proteins. In chapter VI, rabbit sperm proteins were characterised. Six samples were recovered during two months using 5 males from each genotype. Sperm proteins were subjected to nano LC-MS/MS and 487 proteins were identified and quantified. Forty proteins were differentially expressed between genotypes. In conclusion, rabbit sperm proteins showed that genotype has also a huge impact on their abundance. Finally, with these data, the first publicly accessible database of rabbit semen proteome was c / Els objectius generals d'aquesta tesi van ser desenvolupar nous diluents d'inseminació artificial (IA) suplementats amb un anàleg de GnRH i caracteritzar el proteoma del semen de conill. En el capítol I, es va avaluar la inclusió d'un còctel d'inhibidors de proteases en el diluent d'inseminació (DI) per evitar part de l'activitat proteasa del plasma seminal de conill. La qualitat seminal i la fertilitat no es van veure afectades pel còctel. No obstant això, la prolificitat va ser significativament menor en el grup experimental en comparació amb els grups de control positiu i negatiu (8,2 ± 0,22 vs. 9,3 ± 0,23 i 9,2 ± 0,26 catxaps per part, respectivament). D'aquest capítol, es pot concloure que l'addició d'una àmplia varietat d'inhibidors de proteases en el diluent de semen de conill afecta negativament la taxa de prolificitat i que en el futur seria aconsellable provar inhibidors específics de aminopeptidasas (AMIS). En el capítol II, suplementàrem el DI amb AMIs (bestatina i EDTA), i vam estudiar el seu efecte sobre la qualitat seminal i el rendiment reproductiu. La inclusió de AMIs no va afectar la qualitat seminal, ni la fertilitat (85,3 vs. 88,6%), ni la prolificitat (10,12 vs. 10,51 catxaps per part) en comparació amb el grup control. Per tant, concloem que els AMIs es poden utilitzar en els DIs de conill per inhibir part de l'activitat aminopeptidasa del plasma seminal (PS). En el capítol III, vam provar nous DIs de conill que contenien AMIs amb o sense nanopartícules de quitosà (CS)-sulfat de dextrà (DS) que atrapen l'anàleg de GnRH. Es van estudiar els següents diluents: C4 (4 µg de buserelina/conilla en medi control (MC): tris-àcid cítric-glucosa suplementat amb AMIs), C5 (5 µg de buserelina/conilla en MC), Q4 (4 µg de buserelina/conilla en nanopartícules CS-DS en MC) i grup Q5 (5 µg de buserelina/conilla en nanopartícules CS-DS en MC). La fertilitat va ser significativament menor en el grup C4 en comparació amb els grups C5, Q5 i Q4 (0,7 enfront de 0,85, 0,85 i 0,82, respectivament). Per contra, la prolificitat va ser similar en els quatre grups experimentals. Per tant, les nanopartícules de CS-DS com a transportador d'acetat de buserelina permeten reduir la concentració de l'hormona en diluents amb AMIs sense afectar la fertilitat ni la prolificitat. Per això, la nanoencapsulació sembla ser un sistema prometedor per protegir l'anàleg de GnRH en els diluents d'IA de conills. D'altra banda, l'objectiu dels últims tres capítols va ser caracteritzar les proteïnes del semen de conill. En els capítols IV i V, es van estudiar les proteïnes del PS de conill. Les mostres de semen es van recuperar utilitzant 6 mascles de cada línia genètica (A i R) i seleccionant una mostra heteroespérmica del començament, del mitjan i del final de cada estació i de cada línia (24 mostres en total). En el capítol IV, utilitzàrem la tècnica d'electroforesi en gel de poliacrilamida 1D. Set bandes proteiques van ser significativament diferents entre les línies genètiques i tres bandes entre les estacions. En el capítol V, es va sotmetre el PS a nano LC-MS / MS i es van identificar i quantificar 402 proteïnes. 23 proteïnes es van expressar diferencialment entre genotips. Pel que fa a l'efecte de l'estació en el proteoma del PS de conill, els resultats van mostrar que no hi havia un patró clar de variació proteica al llarg de l'any. En el capítol VI, es van caracteritzar les proteïnes de l'espermatozoide de conill. Es van recuperar 6 mostres espermàtiques utilitzant 5 mascles de cada genotip i es van sotmetre a nano LC-MS / MS, identificant i quantificant 487 proteïnes. 40 proteïnes es van expressar diferencialment entre genotips. En conclusió, els resultats dels tres últims capítols evidencien que el genotip està relacionat amb una abundància específica de proteïnes del PS i de l'espermatozoide. Finalment, es va crear la primera base de dades d'accés públic del proteoma / Casares Crespo, L. (2018). Development of new artificial insemination extenders supplemented with GnRH analogues to induce ovulation and proteomic characterization of rabbit semen [Tesis doctoral]. Universitat Politècnica de València. https://doi.org/10.4995/Thesis/10251/100854 / Compendio

This study was designed to : 1) Determine the effects of fresh vs frozen semen at a high inseminate dosage (lOOxl06sperm) contrasted to their effects at a conventional dosage (20xl06 sperm) on accessory sperm per ovum and 2) Evaluate the relationship between accessory sperm number per embry%vum and fertilization status/embryo quality if accessory sperm number were affected by treatment. In this study semen from four bulls routinely giving a minimum of 700/0 morphologically normal and 600/0 motile sperm cells were used. Ejaculates of these bulls were split and prepared for use as fresh and frozen semen at either 100xl06 or 20xI06 cells per dose in.5 mL French straws. Half of the total semen filled straws were frozen in liquid nitrogen at -196°C and half were stored at 5°C for 4 days after collection and used as unfrozen. Cows in standing heat were inseminated with fresh or frozen semen at either high (IOOxl06 sperm) or conventional dose (20xl06 sperm). Ova/embryos were recovered non surgically on day 6 after breeding. Accessory sperm were counted in the recovered embryos/ova after partial digestion with Pronase followed by compression of the embryo/ovum with a cover slip. From 129 inseminations to normally cycling cows, 98 embryos/ova were recovered. To reduce male effects, embryos/ova used were randomly balanced across treatments, by ejaculate within bull for evaluation of frozen vs fresh semen (n = SO) and by bull for evaluation of high vs low dosage treatments (n = 76). No difference (P > 0.05) in accessory sperm was observed for fresh vs frozen semen at either the high or low dosage. The mean accessory sperm values for fresh high dose (n=21), frozen high dose (n=21), fresh low dose (n= 19), and frozen low dose (n= 19) were 26.S1±30.23 (SD), 36.05±44.74 (SD), 29.37± 55.97 (SD) and 30.l6± 70.18 (SD) respectively. When data for embryos/ova resulting from fresh and frozen semen were pooled within dosage, a significant difference was observed between the median accessory sperm values for high and low doses of semen (P < .05). Mean ± SD and median values for accessory sperm were: 37.8± 38.3 and 27.5; 28.9± 62.8 and 3.0, for the high and low dose, respectively. Increasing accessory sperm number by the higher dosage improved the fertilization status/embryo quality (P < .05). Percentage unfertilized ova, degenerate embryos and embryos classified poor to fair and good to excellent were: 3, 5, 24, 68; and 21, 16, 18, 45, for the high and low dose, respectively. Overall, embryos/ova classified good to excellent, poor to fair, degenerate and unfertilized had median accessory sperm values of 18, 9.5, 5.5 and 0, respectively. However, the lack of accessory sperm in unfertilized ova was significantly different from excellent-good quality embryos (P < .05). / Master of Science

Call number: LD2668 .T4 1936 S21

Call number: LD2668 .T4 1985 B74 / Master of Science

M. Tech. Agriculture / South African unimproved indigenous goats are disease tolerant, able to survive on harsh conditions such as extreme temperatures and poor vegetation. It is therefore, important to include this breed in breeding objectives during this time of climate change and when animals are resistant to antibiotics. However, these goats are under threat of extinction and very little information on their reproductive status is recognized. In order to improve or maintain South African unimproved indigenous goats, basic science concerning male fertility, semen quality and production should be investigated to conserve genetic materials for future breeding. Conflicting results have been reported concerning the effect of semen collection techniques on buck sperm quality. Longevity of sperm following semen collection is a major limitation to use fresh semen for artificial insemination in rural communities where majority of South African unimproved indigenous bucks are kept. Extenders such as Tris, egg yolk and cow skimmed milk have been used to prolong the survivability of buck sperm in other countries, but contradictory results have been reported concerning which extender is more suitable for buck semen. Egg yolk based extenders have been used mostly in buck semen compared to Tris or cow skimmed milk based extenders. The purpose of the study was to compare semen collection techniques on South African unimproved indigenous goat semen, and the utilization of indigenous chicken egg yolk for preserving South African indigenous buck semen.

A inseminação artificial (IA) é uma técnica cada vez mais utilizada na suinocultura moderna, pois propicia ao suinocultor vantagens quanto ao desempenho dos reprodutores, ao controle dos cruzamentos e à facilidade na introdução de material genético. Entretanto, a produção de doses inseminantes com qualidade é um dos principais fatores envolvidos no sucesso da técnica. Nesse contexto, a adequada mensuração da concentração espermática do ejaculado suíno é parte fundamental da rotina de trabalho de Centrais de Inseminação Artificial em Suínos. Foram realizados dois experimentos onde se objetivou: Experimento 1 - avaliar a acurácia, a precisão e a robustez do volume do ejaculado do Espermiodensímetro de Karras (ESPM) e do Espectrofotômetro (ESPT), em comparação à Câmara de Neubauer (CN), técnica padrão e também produzir uma tabela de correção de escala do ESPM; Experimento 2 - avaliar o tempo gasto e a repetibilidade de cada técnica, além de comparar a tabela ajustada do ESPM produzida no Experimento 1 com a tabela padrão do ESPM. Utilizaram-se 141 ejaculados, que revelaram concentrações espermáticas médias (milhões de sptz/mL) e número médio de doses que poderiam ser produzidas, respectivamente de 229,1 e 22,6; 185,0 e 18,5; 283,6 e 28,0 para a CN, o ESPT e o ESPM. O viés médio (acurácia) obtido através da média da diferença do resultado da técnica alternativa e da técnica padrão, e o desvio-padrão do viés médio (precisão) do ESPT, em comparação à CN, foram, respectivamente de - 44,1 e 52,6, enquanto que para o ESPM foram de 54,5 e 44,8 (milhões de sptz/mL). Observou-se que o ESPT tende a subestimar e o ESPM a superestimar a concentração espermática, em comparação à CN. O ESPT foi igualmente preciso e mais acurado que o ESPM. Houve pouca influência do volume do ejaculado sobre os resultados do ESPM e do ESPT através dos resultados de robustez para o volume do ejaculado. O ESPM apresentou maior repetibilidade e menor tempo gasto para a realização dos exames, seguido do ESPT e, por último, da CN (P<0,05). A tabela produzida com os dados do Experimento 1 apresentou resultados mais aproximados aos da CN, em comparação com a tabela padrão do ESPM (2,96 bilhões de espermatozóides vivos/dose vs 2,36 bilhões de espermatozóides vivos/dose, respectivamente). Maiores informações de outras Centrais de Inseminação Artificial em Suínos (CIAS) sobre o uso a campo da tabela ajustada produzida, denominada \"Tabela Ajustada para o Espermiodensímetro LPS-FMVZ-USP\", seriam úteis para comprovar a sua eficiência a campo. / Artificial Insemination (AI) in swine is a rising technique that has been used during the last years at the swine production systems, because it furnish several advantages to the producers just like boar high efficiency, better breeding control and easiness in introducing foreign genetic materials. Nevertheless, high quality insemination doses output is one of the most important factors involving the technique success. In this manner, a correct measurement of the spermatic concentration of boars is a basic work routine of the Swine Artificial Insemination Centers (CIAS). Two trials had been performed, whose objectives were: Experiment 1 - evaluate the accuracy, precision and robustness for ejaculation volume of Spermdensimeter (ESPM) and Spectrofotometer (ESPT) in relation to the Neubauer Count chamber (CN), designed as the standard technique. Moreover, produce a scale correction of the ESPM table; Experiment 2 - evaluate the spent time and repetitiousness of each technique, and compare the ESPM adjusted table produced in Experiment 1 with the ESPM standard table. A total of 141 boar ejaculation was used for spermatic concentration measurement by the techniques described above. The average spermatic concentrations (106 sptz/mL) and average doses number were, respectively: 229.1 and 22.6, 185.0 and 18.5, 283.6 and 28.0 for CN, ESPT and ESPM. ESPT mean bias (accuracy) and standard deviation bias (precision), in relation to the CN were, respectively -44.1 and 52.6, while ESPM were 54.4 and 44.8 (106 sptz/mL). Through these results its possible to conclude that ESPT has a tendency to subestimate and ESPM to superestimate the spermatic concentration in relation to the CN. ESPT was more accurate and equally precise than ESPM. Little effect of ejaculation volume on accuracy and precision in both techniques (ESPM and ESPT) was confirmed by the robustness results. ESPM presented a minor coefficient of variation and timeless, followed by ESPT, and finally by CN (P<0,05). The adjusted table produced with Experiment 1 data presented similar results to CN, in relation to the ESPM standard table (2.96 billion of lives sptz/dose vs 2.36 billion of lives sptz/dose, respectively). Complementary data from others CIAS about field use of the ESPM adjusted table produced, named \"Adjusted Table for Karras Spermdensimeter LPS-FMVZ-USP\", would be useful to prove it efficiency.