This paper explores the possibility that Hemingway scholars are overlooking the development of Hemingway as a writer by concentrating too much on the autobiographical elements of his writing. I am not suggesting that scholars ignore the autobiographical aspects of the writing, but rather propose that scholars acknowledge and look for the development of Hemingway's craft of writing in his novels by comparing the early texts with his posthumous works. I have chosen to show this development by comparing A Farewell to Arms and The Garden of Eden, especially through his use of the females characters Catherine in both novels. I assert that whether consciously or unconsciously, Hemingway's reuse of the name Catherine in The Garden of Eden was his attempt to address criticism of his writing by invoking a comparison of the two characters he created in an effort to show how he was able to portray a more sophisticated female character when he was an older, more mature writer. The specific writing tools Hemingway employed to accomplish this task include the use of names, details about the physical appearances of his characters, the vocation of his characters, and the dialogue of his characters.

A district-wide school garden project has been initiated in the Toledo District of Southern Belize in response to reported high rates of poverty and undernutrition. This paper will discuss research conducted in the summer of 2007 with Q'eqchi' Maya in the Toledo District to determine the effect of school gardens on household diet and gardening, the composition of the household diet, and the makeup of homegardens. Food frequency questionnaires and semi-structured interviews were conducted in two rural villages, one with a school garden program and one without. Various members of NGOs working on school garden projects in the district were also interviewed about the functionality and purpose of the projects. Results discussed herein include the limited effects of the school garden program, the role that both homegardens and school gardens play in household diet and nutrition, the diverse array of fruit trees utilized by the Q'eqchi, and a description and inventory of a typical Q'eqchi' homegarden in Belize.

Alzheimer disease (AD) is a progressive neurodegenerative disorder pathologically characterized by deposition of ß-amyloid (Aß) peptides as plaques in the brain. Central to this AD pathology is mismetabolism of the amyloid precursor protein (APP). Recent studies suggest that flavonoids, a class of secondary plant metabolites, may be useful for the prevention and treatment of a variety of neurodegenerative diseases. The studies detailed herein, investigate the ability of two such classes of flavonoids, green tea derived catechins and 5,7-dihydroxyflavones, to modulate APP metabolism in "Swedish" mutant APP (APPsw) models of AD. Studies showed that green tea derived (-)-epigallocatechin-3-gallate (EGCG) effectively reduced Aß generation and resultant amyloidosis both in vitro and in vivo. In concert with these findings, EGCG markedly promoted non-amyloidogenic APP proteolysis via activation of the putative a-secretase, a-disintegrin-and-metalloprotease-10 (ADAM10). Furthermore, luteolin and various related 5,7-dihydroxyflavones, effectively reduced Aß generation and resultant amyloidosis both in vitro and in vivo, as well. Data revealed that luteolin decreased amyloidogenic γ-secretase APP proteolysis via presenilin-1 (PS1) carboxyl-terminal fragment (CTF) phosphorylation. Elucidation of these flavonoids' cellular/molecular mechanisms also revealed their potential for opposing neurofibrillary tangle (NFT) pathology, another hallmark of AD. These data raise the possibility that flavonoid administration to AD patients may prove to be viable and effective prophylactic strategy.

America's embrace of film noir came swift and furiously, the popularity of noir exists even in contemporary cinema. I would like to explore the implications as to why film noir has become one of the truest forms of American Cinema, perhaps even exceeding the western, as well as the reasoning as to why the American people have exalted a type of genre which is known primarily for its ties with human vice and depravity. In this investigation of the populations intrigue with noir I will address instances in select noir films that illustrate specific moments of the philosophical frame works of Michel Foucault. Through the application of these frameworks of thought I believe evidence can be found linking Film noir to primal human urges and desires that were initially discussed within the writings of these two philosophers. Throughout the evolution of cinema over the last 70 years, America has seen an abundance of reconditioned plots and outlines of classically structured stories. Film noir does not escape this refurbishment. With the collapse of the original Hollywood studio system as well as the infamous black list era, the ideology of Film making in America shifted enormously. This shift allowed cinema to reach into the postmodernist conditioning that had already been applied to literature and stage craft. The shift into postmodernism allowed for extraordinarily interesting developments in the genre of Film noir. Perhaps the most noted of these developments was that noir was no longer just a genre; it had become an actual ideology for telling a cinematic story. This is exemplified with the emergence of noir sensibilities throughout multiple contrasting film genres. This is illustrated throughout the arrival of such categories as the Science Fiction Noir, and most recently the genre of Neo-Noir. Neo-Noir is also home to the films that have attempted to satirize or parody the initial sensibilities of the original classic noir genre. The exploration of these new evolutions of noir constructed genres is of vast importance of understanding America's embrace of Film noir as a whole.

NADH:Cytochrome b5 Reductase (cb5r) catalyzes the reduction of the ferric iron (Fe3+) atom of the heme cofactor found within cytochrome b5 (cb5) by the reduction of the FAD cofactor of cb5r from reducing equivalents of the physiological electron donor, reduced nicotinamide adenine dinucleotide (NADH). Cb5r is characterized by the presence of two domains necessary for proper enzyme function: a flavin-binding domain and a pyridine nucleotide-binding domain. Within these domains are highly conserved "motifs" necessary for the correct binding and orientation of both the NADH coenzyme and the FAD cofactor. To address the importance of these conserved motifs, site-directed mutagenesis was utilized to generate a series of variants of residues located within the motifs to allow for the full characterizations. Second, naturally occurring recessive congenital methemoglobinemia (RCM) mutants found in proximity to these highly conserved motifs were analyzed utilizing site-directed mutagenesis. In addition, a canine variant of the cb5r soluble domain was cloned, generated and characterized and compared with the WT rat domain. The canine construct showed a high degree of sequence homology to that of the corresponding human and rat sequences. Characterization of the canine variant indicated that it possessed comparable functional characteristics to the rat variant. Investigation of the pyrophosphate-associating residues, Y112 and Q210, indicated that each played a role in the proper association and anchoring of NADH to the enzyme. The RCM type I mutants, T116S and E212K, caused a moderate decrease in efficiency of the enzyme. The presence of both mutations interact synergistically to generate a more substantially decreased function Analysis of the "180GtGitP185" NADH-binding motif and the preceding residue G179 revealed that these residues are vital in enabling proper NADH association. The residues of this motif were shown to be important in determining nucleotide specificity and properly positioning the NADH and flavin cofactor for efficient electron transfer. RCM variants A178T and A178V were shown to decrease catalytic efficiency or protein stability respectively, leading to disease phenotype. Analysis of the NADH-binding motif "273CGxxxM278" indicated that this motif facilitates electron transfer from substrate to cofactor and is important in release of NAD+ from the enzyme after electron transfer.

Metalloporphyrins have been shown to catalyze many fundamental and practically important chemical transformations, some of which represent the first demonstrations of these catalytic processes. The most notable examples include an assortment of atom/group transfer reactions, such as oxene, nitrene, and carbene transfers. Atom/group transfer reactions allow for the direct conversion of abundant and inexpensive alkenes and alkanes into value-added functional molecules. Previous reports from our group have shown that cobalt-porphyrin based carbene and nitrene transfer reactions are some of the most selective and practical catalytic systems developed for cyclopropanation and aziridination. Backed by a family of D2-symmetric chiral cobalt porphyrins our group continues the development of stereoselective carbene and nitrene transfer reactions. Metal-catalyzed cyclopropanation of olefins with diazo reagents has attracted great research interest because of its fundamental and practical importance. The resulting cyclopropyl units are recurrent motifs in biologically important molecules and can serve as versatile precursors in organic synthesis. Supported by a family of D2-symmetric chiral cobalt porphyrins, we have demonstrated the use of succimidyl diazoacetate as carbene source for a highly diastereo- and enantioselective cyclopropanation process. The resulting cyclopropyl succinimdyl esters are highly reactive and serve as valuable synthons for generating cyclopropylcarboxamides. We have also developed the first cobalt-porphyrin based intramolecular cyclopropanation, which is able to produce the resulting bicyclic lactones in high yields and enantioselectivity. Nitrene transfer reactions are also an attractive route to produce biologically and synthetically important molecules such as amines and aziridines. Although much progress has been made in nitrene transfer reactions utilizing [N-(p-toluenesulfonyl) imino]phenyliodinane (PhI=NTs) the nitrene source suffers from several drawbacks. Consequently, there has been growing interest in developing catalytic nitrene transfer reactions using alternate nitrene sources. To this end, we have utilized arylsulfonyl azides as nitrene source to explore their use in the development of a cobalt-porphyrin catalyzed enantioselective aziridination system. The cobalt catalyzed process can proceed under mild and neutral conditions in low catalyst loading without the need of other reagents, while generating nitrogen gas as the only byproduct. We have also explored the use of arylsulfonyl azides as nitrene source in a cobalt-catalyzed intramolecular C-H amination process.

As a comparison to the suction feeding mechanics in aquatic environments, I investigated buccal pumping in an ontogenetic series of suspension feeding Xenopus laevis tadpoles (4-18 mm snout-vent length) by examining the morphology, kinematics, fluid flow, pressure generated in the buccal cavity, and effects of viscosity manipulation. Investigation of the dimensions of the feeding apparatus of Xenopus revealed that the feeding muscles exhibited strong negative allometry, indicating that larger tadpoles had relatively smaller muscles, while the mechanical advantage of those muscles did not change across the size range examined. Buccal volume and head width also exhibited negative allometry: smaller tadpoles had relatively wider heads and larger volumes. Tadpoles were imaged during buccal pumping to obtain kinematics of jaw and hyoid movements as well as fluid velocity. Scaling patterns were inconsistent with models of geometric growth, which predict that durations of movements are proportional to body length. Only scaling of maximum hyoid distance, duration of mouth closing, and duration of hyoid elevation could not be distinguished from isometry. The only negatively allometric variable was maximum gape distance. No effect of size was found for duration of mouth opening, duration of hyoid depression, and velocity of hyoid elevation. Velocity of mouth opening, velocity of mouth closing, and velocity of hyoid depression decreased with increasing size. Fluid velocity increased with size, and is best predicted by a piston model that includes head width and hyoid depression velocity. Reynolds number increased with size and spanned two flow regimes (laminar and intermediate) ranging from 2 to over 100. Pressure was found to be greatest in the smallest tadpoles and decreased as size increased, ranging from 2 kPa to 80 kPa. The viscosity of the water was altered to explore changes in body size, independent of development (higher viscosity mimicked smaller tadpole size). Viscosity manipulations had a significant effect on the kinematics. Xenopus initially increased velocity and distance of movements as viscosity increased, but these values declined as viscosity increased further. These results suggest that abiotic factors such as fluid viscosity may set a lower size limit on suspension feeding.

There is an underlying hope that those whom are elderly can exist in a place of happiness, comfort, security and familiarity; and can do so by aging in a place which complies with an understanding of their needs as older adults. However, along with aging comes a negative association of dependence and deterioration. Therefore, it is intuitive to have a connection between both the aging process and the design process. Creating a product which brings a positive association with senior housing and aging in place is the underlying goal within the project and overall design. With the increasing population of those 65 and older, there is a higher need for the placement of individuals who can no longer live independently or those who do not have family available to care for them. More specifically, the views of caring for elderly within Latin American cultures have a highly negative connotation towards the placement of older relatives outside of their proper family home. So how does the problem of increasing need for senior housing become solved with those who reject the idea of senior housing facilities and nursing homes? In order to change the perception of senior housing within Latin America, there needs to be an element of identity and familiarity to the home. It is that sense of identity and familiarity which will produce a product of belonging and comfort that promotes a positive lifestyle within senior housing. In order to directly address the Latin American culture, the proposed site will be located in an island which shares both United States values and views along with their own rich culture and history. Designing within the tropical conditions and cultural traditions of Puerto Rico will give its elderly a new and essential residential typology. It is extremely important to choose an environment which is beginning to acknowledge the change in demographic and economy that requires a more positive form of assisted living facilities and senior housing. Through a methodology which involves analyzing Puerto Rican cultural elements and conditions, the thesis will result into an understanding of cultural spatial traditions and customs. Accordingly, by taking these cultural conditions and implementing them into an senior housing program, it will bring a sense of home and place which is not found in the majority of elderly housing facilities within Puerto Rico.

Curcumin is the yellow pigment found in the Indian spice curry. It has anti-inflammatory, ant-oxidant, anti-cancer, anti-viral, anti-bacterial and wound healing properties. It is widely used in industry for its flavor as a spice and as a coloring agent because of its brilliant yellow color. It is also used as a dye for textiles and as an additive to cosmetics. Dendritic cells (DCs) are the sentinels of the immune system and functions as the bridge between the innate and adaptive immune response. The effect of curcumin on DCs is poorly understood. A study shows curcumin prevents the immuno-stimulatory function of bone marrow-derived murine DCs, but no study examines the effects on human DCs. This study investigates the effects of curcumin on immature human DC maturation and function in response to immune stimulants lipopolysaccharide (LPS) and polyinosinic-polycytidylic acid (poly I:C). Human CD14+ monocytes isolated from the peripheral blood of donors are cultured with GM-CSF and IL-4 supplemented media to generate immature DCs. The cultures are treated with curcumin, stimulated with the above mentioned stimulants then functional assays performed. These assays include homotypic cluster formation, surface marker expression, cytokine production, chemotaxis, endocytosis, DC-induced allogeneic CD4+ T cell proliferation after mixed lymphocyte reaction, gene expression analysis and immuno-fluorescence labeling and imaging. Curcumin-induced changes in gene expression indicate the actin cytoskeleton signaling pathway is a target. Immuno-fluorescence labeling and imaging of f-actin was carried out. Curcumin reduces DC maturation in response to the stimulants used in the study. Expression of surface markers, cytokines and chemokines is reduced as well as DC-induced stimulation of allogeneic CD4+ cells after MLR. Curcumin prevents chemotaxis without affecting chemokine receptor expression and significantly reduces endocytosis in non-stimulated cells. Curcumin-treated DCs do not induce a Th1 or Th2 population in allogeneic MLR but induces a CD25+Foxp3+ regulatory cell population. Immuno-fluorescence imaging shows curcumin causes the cell to become more rounded. These data imply that curcumin inhibits f-actin polymerization and thereby prevents DC maturation and function in response to stimulation. This outlines a novel role for curcumin as an immune suppressant and shows its therapeutic potential as an anti-inflammatory agent.

Background: The traditional model of infection control has failed to stop the spread of emerging infectious diseases such as Clostridium difficile-associated diarrhea (CDAD) in the acute care environment. Ecological models, which rely upon identification of susceptible hosts, offer an alternative to the prevention of deadly outbreaks. Previous epidemiological research has identified a number of risk factors associated with CDAD. Utilization of this body of research by nurses is limited due to methodological issues that introduce bias and confounding, and use of variables that have limited meaning to the practicing clinical nurse. Aim: The aim of this study was to develop an ecological model useful for nurses in predicting the susceptibility of individuals to CDAD during an acute care hospital stay. Method: A case-control study compared 66 cases with CDAD to 66 controls matched for the temporal and spatial risk factors of hospital admission date and geographic nursing care unit within the institution. The two subject groups were compared on variables of age, antibiotic burden, laxative or bowel preparation exposure, nutritional status, gastric acid suppression therapy, enteral feeding exposure, and severity of illness as measured on the Horn Severity of Illness index. All subjects were hospitalized between January 1, 2000 and December 31, 2006. Results: On univariate analysis, age, severity of illness, serum albumin levels, length of exposure, and proton pump inhibitor drug burden were significantly associated with CDAD status. Following multivariate analysis, only severity of illness, length of exposure, and decreased antibiotic drug burden were significantly associated with the development of hospital-acquired CDAD. Conclusions: This study supports the use of an ecological perspective in identifying risk factors and interventions to prevent the future spread of this infectious disease.