The Tampa River Walk project is one of great importance for revitalization of the waterfront of downtown Tampa. This Riverfront development will be even more important when it becomes a vital example of how a riverfront can stretch and pull together downtown Tampa and its surrounding areas: Hyde Park, Harbour Island, and Historic Ybor. The purpose of this master's project is to explore an ecological ex pansion design approach for the Tampa River Walk as a master plan and then zooming into to an area to design in detail of what the riverfront can be. It will start by concentrating on the areas that were not dealt with in the proposal by EDAW: the west side of Hillsborough River, and areas that are immediately adjacent. Furthermore, it will investigate future expansion of the River Walk as downtown Tampa potentially expands and connects out to the suburbs in thirty to forty years. The main focus of this project will be to create an iconic design that gives the Tampa's riverfront a character and identity. This unique characteristic will be created by knitting nature into the urban fabric and using the River Walk as a natural seam. The connection throughout the riverfront will be made by a natural green strip. It will explore the idea of stretching and pulling of public zones vertically and horizontally to create connectivity and identity with the River Walk. Two resarch methods that will play important roles in this investigation are Design and History Research, and Case Study and Multi-method Approaches to Research. The research timeline will concentrate on mainly the history of development and use of Tampa's riverfront starting from 1600's when Tampa Bay was discovered by Spanish explorers, through the River Walk development proposed today. Also, case studies of Chicago Water Front, San Antonio River Walk, and others will inform various strategies taken in different geographical locations and impacts they've had on the growth of the cities. This project will provide an expansion design approach that is not only a concern for the present time, but also looking ahead at what it might provide for the future. The result of this project will hopefully serve as a stepping stone for a new way of revitalizing Tampa's downtown and surrounding areas through knitting of nature into an urban fabric.

I intend to showcase Suzan-Lori Parks's repetitious, supplemental virtuosity, which is a testament to the fluid, indeterminate condition of her concepts. I seek to demonstrate that in textualizing the quality of absence in the written dialogue, Parks's The America Play becomes uniquely deconstructive. For indeed, through the absent "presence" of an historically mythified president and a gravedigger's skewed identity, the play becomes a stage for splinters of historicized, differentiating, repeating signifiers that supplement, even as they redefine, their referential signified. Performing deconstructive thinking, Parks's textuality is accommodated by a content through which she calls attention to the structures of metaphysics in our discourse, stressing our inability to erase them, and our need to question them through continual self-reflexive thinking. I hope to show that Parks's genius is apparent in her unique mastery of language. This kind of mastery is revealed through her drama's connection to conceptual possibilities, which are hypostatize, staged through the materiality of her drama's textual configuration. In consciously imbuing her pages with representational temporal spaces akin to Derrida's différance, Parks shows how the supplemental perpetuity of metaphysical signification phenomenally attests to the conceptual, idealized absence it supplements. I speak here of idealization that underlies the structural signified, always already a supplemented absent, which carries metaphoric trappings of phenomenological substance in a form of the signifier, returning through a morphemic ideality in the assuredness of its infinite return. I read Park's intent in The America Play as inherently deconstructive because Parks dramatizes the enigma of the trace; that is, the enigma of its impossible, yet, relentless repetition. In examining the trace and its historical "genesis" through the Idea in the Kantian sense, I will show how Parks's Rep & Rev, repetitions and revision, textually performs its impossible repetition, which is always metaphysical. In self-reflexively showing the impossibility of metaphysical presences, Parks establishes a need for a persistent practice of deconstructive interrogation, questioning self-assured, metaphysical, dogmatic thinking.

The objective of this study was to address the relationship between benthic microalgal communities and the phosphate nutrient dynamics of Florida Bay sediments and how they relate to benthic and water column primary production. In situ phosphate (P) flux between the sediment and the water column was measured in three regions of Florida Bay. Differences in the ratio of inorganic to organic phosphate flux were found between the three regions in relation to the amount of phosphate measured in the water column. Based upon the average sediment flux in my study, more than 1600 metric tons of P would be supplied by the sediment per year in Florida Bay. Based upon my measurements, dissolved nutrient flux from the sediment can be an important contribution to pelagic phytoplankton blooms in Florida Bay, accounting for 6.5 - 41% of demand and TDN accounts for 100% of the N demand. My findings were similar to others for both benthic nutrient flux and benthic microalgal chlorophyll a concentration. Benthic microalgae in Florida Bay contribute 700 kg Chl a per day to the system. Mesocosm experiments demonstrated that benthic microalgae and water column phytoplankton can respond differently to changes in nutrient availability. The dissolved nutrient in least supply in the water column does not necessarily correspond to the limiting nutrient for benthic microalgae. ³³P acted as a tracer between sediment and water column dissolved P pools. The presence of benthic microalgae enhanced the transport of ³³P to the water column as compared to simple Fickian diffusion. This was supported by the positive flux of dissolved P from the sediment to the water column pools in control treatments with a living benthic microalgal layer. Primary production by benthic microalgae were measured using dissolved O2 evolution and PAM fluorometry. Primary production for BMA habitat in Florida Bay was between 400 and 800 tons of C per day, based upon O2 production and PAM fluorometry, respectively.

Prior research has attempted to develop a model of organizational citizenship behaviors (OCB) and counterproductive work behaviors (CWB), but limited testing remains a problem. The purpose of the current study is to examine OCB and CWB from a job stressor-strain approach. The sample consisted of 235 employees throughout the United States and their supervisors. Results of the study suggested OCB and CWB are affected by stressors (including interpersonal conflict, low interactional justice, job demands, and organizational constraints). Additionally, trait emotion and attributional styles affect the amount of stressors perceived. The implications as well as limitations of the study are discussed.

The goal of this dissertation was to further examine the visual system and its importance to the bonnethead shark (Sphyrna tiburo). This species of hammerhead shark possesses the least amount of lateral cephalofoil expansion. Better understanding of their visual system and potential visually-mediated behaviors may increase understanding regarding adaptive benefits of their unique head shape. The dissertation revealed four factors regarding this species' visual system: 1) the extent of their optical visual fields span between 68-72 degrees laterally and cover their visual horizon, 2) they possess a fairly large (approximately 112 degree) blind spot directly in front of their cephalofoil, 3) they possess an average of 35 degrees of lateral head movement during sinusoidal swimming which likely increase the lateral extents of their optical visual fields, and 4) they can detect and show interest in small visual stimuli resembling their preferred prey species, the blue crab.

The emergence of West Nile virus (WNV) has raised important questions about the capacity of the public health infrastructure to implement surveillance and control programs for WNV and other emerging or re-emerging arboviruses in the United States. Florida's mild climate supports year round enzootic transmission of WNV, St. Louis encephalitis virus (SLEV), and Eastern Equine Encephalitis virus (EEEV). It is unknown what effect the establishment of WNV (in 2001) will have on SLEV transmission in Florida, where these closely related flaviviruses share amplifying hosts, habitats, and vectors. An Arbovirus Isolation Network was formed to obtain and characterize arbovirus strains collected from a large population of naturally exposed birds, including sentinel chickens and wild birds admitted to rehabilitation centers in Florida. Weekly sentinel seroconversion data was used to target sampling of chicken flocks at 37 active sites (17 WNV, 7 EEEV, and 13 SLEV) in eight counties from 224 birds during 2005-2006. Sampling of wild birds occurred following admittance at rehabilitation centers in 2006, based on symptoms and known amplifying host species (n=64), but virus was not detected. We report the isolation of St. Louis encephalitis virus, West Nile virus and detection of Eastern Equine Encephalitis viral RNA from cloacal swabs of naturally exposed adult sentinel chickens. We also report the first known dual infection and isolation of St. Louis encephalitis and West Nile viruses from one chicken. In addition, a novel flavivirus strain was detected in two chickens. Early season transmission of WNV appears to limit subsequent infection and amplification of SLEV late in the year. Phylogenetic analysis revealed that the introduction (and re-introduction) of South American (Brazil) SLEV occurred in 1972 and 2006 in Florida. These strains represent the first reported isolation of South American strains of SLEV in the United States, with placement in Lineage VA and VB, as proposed by Kramer and Chandler (2001). Arbovirus isolation remains an effective tool for surveillance programs and a targeted strategy is most cost-effective to capture arboviruses in their natural settings for molecular epidemiology analysis that can elucidate genetic variations impacting virulence, mosquito infectivity, and disease potential of these pathogens.

Previous study of drilling predation on the bivalve Chione during the late Neogene of Florida suggested that prey-size selectivity of predators was disrupted by species turnover and morphological change within the prey genus. More recent experimental work, however, showed that at least some of these changes can be attributed to the confounding effects of facies shifts between naticid-dominated, muricid-dominated, and mixed predator assemblages. As muricids have the most abundant and continuous fossil record and are most responsible for predation on the Chione bivalve in modern benthic ecosystems of Florida, we use new criteria to isolate the muricid component of the Chione drillhole record and analyze the history of this type of predator independently. Our analysis, based on drilled Chione from four Plio-Pleistocene formations in Florida, does not support the previous scenario of disruption at the end of the Pliocene followed by predator recovery. Rather, selected prey size has steadily increased since the middle Pliocene, although the stereotypy of prey-size selection behaviors has decreased. In order to explain this trend, I performed a series of statistical analyses to explore factors most likely to have influenced muricid prey-size stereotypy. The timing of Species turnover within the prey lineage or change in prey phenotype does not correlate with the timing of changes in prey-size stereotypy and, therefore, cannot explain the observed changes in muricid behavior. Presence of secondary predators may also influence predator-prey interactions, because predators forage sub-optimally to ensure greater safety in the presence of enemies. Results indicate that secondary predation pressure decreased at the Caloosahatchee-Bermont boundary without any evident change in muricid prey-size stereotypy and hence refute the hypothesis that secondary predation induced sub-optimal foraging. A third factor tested is prey density, which plays a major role in predator-prey interactions in other systems by thwarting a predator's ability to single out the preferred individual prey. Increased Chione prey density correlates with and provides support for increased confusion among the muricid predators and hence driving the increased sub-optimal behavior reflected by the increased variability in prey-size selection. This is the first time prey density effect has been considered and its importance here over all other factors suggests that it may be a critical factor in short- and long-term predator behavior trends in fossil record.

The role of the Strength & Conditioning coach (SCC) has increased dramatically in collegiate athletics over the past 30 years. The SCC now spends more time with the athletes than even the individual sport coaches do because of NCAA rules. Despite the importance of the SCC, little is known as to what makes a good SCC and what a typical SCC is like currently. Limited amounts of research have been conducted to determine the characteristics and opinions of this specific population. The main role of a SCC is to enhance athletic performance of the athletes at a university. They achieve this goal by enhancing strength, power, speed, agility, conditioning, flexibility, among other things. In addition, a good SCC will also help "toughen" up a team mentally, consult athletes on nutrition facts, and serve a variety of roles during team practices. The purpose of this study was to survey NCAA Division I (bowl subdivision) SCCs to assess what characteristics they possess as well as what characteristics they deem to be important for other SCCs to possess. The questions asked ranged from education level to current activity level. The results of the current study supported the hypotheses. SCCs come from a variety of backgrounds in regards to their education, certifications, past experiences, physical activity level, and physical size. The coaches also tended to favor other coaches similar to themselves. With the findings from this study, prospective SCCs will have a better understanding of the hiring practices of prospective employers. Current SCCs will gain a better knowledge of their peers and the field in general. Future research is needed in the field regarding race and gender, two topics only briefly discussed in the current investigation.

Coral cover remains highest on patch reefs at the northern end of the Florida reef tract. The reasons for this trend are not well understood, but may be related to the protection from extreme variations in water quality parameters provided by the near constant presence of islands at the north extent of the Florida Keys. Three indices have been developed based on Foraminifera and sediment constituents. Two of the indices, the FORAM Index and the SEDCON Index, were developed to indicate the suitability of a reef environment for continued reef accretion. The third index, the Photic Index, is an assessment of photic stress on reefs based on incidence of bleaching in a species of Foraminifera, Amphistegina gibbosa, which is known to experience loss of algal endosymbionts similar to bleaching in corals. Patch reefs were sampled in Biscayne National Park, FL to assess sediment characteristics and foraminiferal assemblages, as well as to examine trends in the three indices. Sediments associated with a majority (59%) of reefs were coarse sands; muddy sediments were restricted to a few inner patch reefs that were isolated from the influence of Caesar's Creek, which flushes water from inside Biscayne Bay onto the open shelf. Unidentifiable grains predominated in the sediment constituents, along with calcareous algae and molluskan debris. Shells from 82 genera of Foraminifera were identified in the sediments. Quinqueloculina was the most consistently common genus. Percent mud was the single most influential measured variable on the distribution of both sediment constituents and foraminiferal assemblages. Analysis of bleaching in the foraminifer Amphistegina gibbosa revealed that photo-oxidative stress was chronic at 94% of the sites. Patterns of FORAM and SEDCON Index values and their similarity to temperature, salinity, and percent mud distributions show that Caesar's Creek is affecting the benthic community in its immediate vicinity by providing flow that limits the accumulation of mud and potentially other anthropogenic stressors. Overall this study suggests that the reefs in this area are marginal for continued reef growth. A more detailed study of water quality through Caesar's Creek should be conducted to determine exactly how it is affecting the reefs in Biscayne National Park.

In this paper, a parametric study of the airbrush deposition technique was investigated for the deposition biomolecular thin films. The airbrush parameters under investigation were intake valve opening, carrier gas pressure, distance between the airbrush and substrate, concentration of solution, vapor pressure of solvent, and hydrophobic/hydrophilic substrate surface. This study was assessed through the characterization of dried droplet residues of Bovine Serum Albumin (BSA) and of complete films of BSA by means of scanning electron microscopy (SEM) and atomic force microscopy (AFM). It was determined that droplet size was mainly affected by carrier gas pressure and vapor pressure. The parameters intake valve opening, distance between the airbrush and substrate, and concentration of solution control the rate of spray, or solution flux, onto the substrate. Solution flux was determined to have the greatest impact on film roughness. This allowed for flexibility in the airbrush deposition technique to produce films with various substrate wetting rates. Low flux films were produced when the droplets dried on the substrate surface before the next droplet arrived. High flux films were generated when droplets on the surface arrive before subsequent droplets are given time to dry. Finally, as an extension of the results of these experiments, a practical application of the airbrush deposition technique was conducted using appropriate deposition parameters. An E. coli wave guide biosensor was produced on a glass substrate. A sandwich immunoassay was used to confirm E. coli capture.