Submitted by Ronildo Prado (ronisp@ufscar.br) on 2017-08-10T14:10:10Z No. of bitstreams: 1 DissWIF.pdf: 2975197 bytes, checksum: 0039c1cb759b3d42c9bd5b659146af6a (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-08-10T14:10:20Z (GMT) No. of bitstreams: 1 DissWIF.pdf: 2975197 bytes, checksum: 0039c1cb759b3d42c9bd5b659146af6a (MD5) / Approved for entry into archive by Ronildo Prado (ronisp@ufscar.br) on 2017-08-10T14:10:28Z (GMT) No. of bitstreams: 1 DissWIF.pdf: 2975197 bytes, checksum: 0039c1cb759b3d42c9bd5b659146af6a (MD5) / Made available in DSpace on 2017-08-10T14:10:35Z (GMT). No. of bitstreams: 1 DissWIF.pdf: 2975197 bytes, checksum: 0039c1cb759b3d42c9bd5b659146af6a (MD5) Previous issue date: 2013-08-26 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / The nucleus of the solitary tract (NTS) is the primary synaptic site of the peripheral baroreceptors and chemoreceptors. It has been shown that acetylcholine (ACh) microinjected into the NTS of rats induces hypotension and bradycardia. However, the contribution of cholinergic mechanisms at different NTS subnuclei (intermediate and commissural) as well as the cholinergic receptors blockade on the control of sympathetic (SNA) and phrenic (PNA) nerve activities have not been studied yet. In this study we assessed the role of ACh and its cholinergic receptors at the intermediate NTS (iNTS) and commissural NTS (cNTS) on the control of SNA, PNA and electrophysiological properties of these subnuclei neurons, as well as on baro and chemoreflex responses. Decorticated arterially-perfused in situ preparations of male juvenile rats were used to record SNA and PNA. Microinjections of ACh and cholinergic antagonists were performed into the iNTS or cNTS. Coronal slices of the brainstem containing either cNTS or iNTS subnuclei were obtained from male juvenile rats and used in whole cell patch clamp – current clamp recordings. It was observed that ACh microinjected into the iNTS inhibited both SNA and PNA. These effects were reduced by the pre-treatment with atropine (muscarinic antagonist) or mecamylamine (nicotinic antagonist). The cholinergic antagonists into the iNTS did not change the effects on SNA and PNA induced by baro and chemoreflex activation. In contrast, microinjections of ACh into the cNTS did not induce changes in SNA, but increased PNA. Despite the absence of changes in SNA, ACh into the cNTS changed the pattern of respiratory-sympathetic coupling. Both atropine and mecamylamine into the cNTS inhibited the ACh-induced tachypnea, but only mecamylamine inhibited the chemoreflex-induced tachypnea and the ACh-induced change in respiratory-sympathetic coupling. In vitro studies demonstrated that ACh promotes depolarization in both iNTS and cNTS neurons. Both muscarinic and nicotinic antagonism in the iNTS inhibited the ACh-induced depolarization. However, only nicotinic antagonist was effective in diminishing this response in the cNTS. The results suggest that ACh plays an important role in the control of cardiovascular and respiratory activities, with distinct functions between iNTS and cNTS. This cholinergic control involves activation of both muscarinic and nicotinic receptors within NTS, but only nicotinic receptors are involved in the chemoreflex tachypneic response. / O núcleo do trato solitário (NTS) é o sítio primário de aferências dos barorreceptores arteriais e quimiorreceptores. Sabe-se que a acetilcolina (ACh) injetada no NTS de ratos provoca hipotensão e bradicardia. Entretanto, não se sabe até o momento qual o papel do sistema colinérgico nos diferentes subnúcleos do NTS (intermediário ou comissural) ou o bloqueio de seus receptores na atividade simpática (SNA) ou na atividade do frênico (PNA). No presente estudo avaliamos os efeitos da ACh e seus receptores no NTS intermediário (NTSi) e comissural (NTSc) sobre a SNA, PNA e sobre as propriedades eletrofisiológicas dos neurônios desses subnúcleos, bem como nas respostas do baro e quimiorreflexos. Preparações in situ decorticadas de ratos jovens foram utilizadas para registro da SNA e PNA, e ACh e antagonistas colinérgicos foram microinjetados no NTSi ou NTSc. Cortes coronais bulbares contendo o NTSi ou NTSc foram obtidos de ratos jovens e utilizados para registro de neurônios através da técnica whole cell patch clamp – current clamp. Foi observado que a microinjeção de ACh no NTSi inibe tanto a SNA quanto a PNA, sendo que tais efeitos são inibidos pelo tratamento com atropina (antagonista muscarínico) ou mecamilamina (antagonista nicotínico). Os antagonistas colinérgicos no NTSi não alteraram os efeitos na SNA e PNA induzidos pela ativação do baro e quimiorreflexos. Por outro lado, microinjeções de ACh no NTSc não altera a SNA mas promove aumento da PNA. Apesar de não alterar a SNA, a ACh no NTSc promove alterações no acoplamento simpato-respiratório. Tanto a atropina quanto a mecamilamina microinjetadas no NTSc inibiram a taquipnéia induzida pela ACh, mas apenas a mecamilamina inibiu a taquipnéia resultante do quimiorreflexo e a alteração no acoplamento simpato-respiratório induzida pela ACh no NTSc. Nos estudos in vitro, observamos que a ACh promove respostas de despolarização em neurônios tanto do NTSi quanto do NTSc. O bloqueio de receptores tanto nicotínicos quanto muscarínicos no NTSi diminui a despolarização evocada pela ACh. No entanto, apenas o antagonista nicotínico foi capaz de diminuir essa resposta no NTSc. Os resultados sugerem que a ACh desempenha um importante papel no controle cardiorrespiratório, com funções distintas entre NTSi e NTSc. Esse controle colinérgico envolve a ativação de receptores tanto nicotínicos quanto muscarínicos no NTS, mas apenas receptores nicotínicos estão envolvidos na resposta de taquipnéia do quimiorreflexo. / FAPESP: 2010/17218-0