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Declining bat populations necessitates a need to understand how different land management techniques influence bat activity. This study assessed the influences of different coastal upland habitat management techniques, such as mulching, prescribed fire, and select cut, on forest bat activity within the Grand Bay National Wildlife Refuge and National Estuarine Research Reserve. Acoustic recorders were used to monitor bat activity and insect and vegetation surveys were used to assess influences on bat activity across different land management techniques. Results demonstrate that overall bat activity was similar across different land management techniques, however larger species adapted for open-space flying were shown to be less active within dense forest such as the select cut technique areas. Findings from this study suggest that various land management techniques can influence bat activity differently.

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Human activities are a major driver of biodiversity degradation and loss, especially in tropical forest areas, where forest-fringe towns and villages depend on the forests for their livelihoods. In order to reduce threats that human activities pose to biodiversity, livelihoods support programs are employed as economic incentives for biodiversity conservation. These programs support the livelihoods activities of local communities, with the aim of triggering favorable attitudes and behaviors towards conservation, and ultimately reduce biodiversity degradation. Their effectiveness as conservation tools has not been evaluated. I investigated the effects of livelihoods programs on conservation attitudes and the consequent effects on biodiversity in the Afadjato-Agumatsa and Atewa forest areas in southeastern Ghana. The study areas are coupled human and natural systems, which are excellent for research in the theoretical framework of biocomplexity in the environment. Using literature reviews and field visits, I documented the specific livelihoods support activities (LSAs) used for biodiversity conservation, their historical trend and geographical distribution in Ghana. I used ex-post costbenefit analysis to determine socio-economic estimates of the LSAs in the two forest areas. Since communities were not randomly assigned to the interventions, I employed quasi-experimental design to evaluate the effects of LSAs on environmental attitudes. I evaluated the effect of conservation attitudes on biodiversity at two levels. These levels included 1) functional biodiversity at the landscape level represented by mean Normalized Difference Vegetation Index (NDVI) of forest; and 2) compositional biodiversity at the species level represented by species diversity of fruit bats. iv The earliest record of LSAs used for biodiversity conservation in Ghana was in 1993. I identified 71 different activities belonging to eight categories. Some of these activities are beekeeping, animal husbandry, crop farming, and snail rearing. Most LSA programs have been in northern Ghana. There was an increasing tendency to make LSAs part of every conservation program in Ghana and this satisfies the current policy of collaborative conservation. The socio-economic estimates of LSAs included: 1) capital investment; 2) net socio-economic benefits; and 3) the benefit-cost ratio. The per-community values of the three estimates were not different between the two study areas. The per capita values of capital investment and net economic benefit were not significantly different between the two study areas. However, benefitcost ratio per capita was higher in Afadjato-Agumatsa than in Atewa. Estimates of economic returns from LSAs were marginal but the perceptions of success were relatively high. Environmental attitudes in LSA communities and non-LSA communities were not significantly different, and this was confirmed by an estimate of infinitesimal effects of LSAs on forest conservation attitudes. Among LSA communities, benefit-cost ratio of LSAs predicted favorable forest conservation attitudes; and change in pro-conservation attitudes were significantly higher in communities that had active LSAs than in communities which had no active LSA. Mean NDVI of the forests decreased from 1991 to 2000 and decreased further but at a slower rate to 2010. Higher forest conservation attitudes predicted higher mean NDVI in 2010. Higher change in mean NDVI from 1991 to 2000 predicted higher change in mean NDVI from 2000 to 2010. Eleven of the 13 fruit bat species in Ghana were recorded in the study areas. Longer v distances between a local community and its forest predicted higher species diversity of forestspecialist fruit bats. The results indicate that LSAs have become a major contribution to Ghana’s current collaborative forest policy. The fact that perceptions of LSA success were moderate even though the economic returns from them were marginal suggest that other factors such as provision of employment, training in new skills and community cohesion played a part in how communities viewed the success as LSAs. Evaluations of conservation attitudes suggest that just participating in LSAs did not improve attitudes; but higher benefit-cost ratio predicted favorable conservation attitudes, and conservation attitudes were higher in communities that sustained their LSAs. Therefore, it may serve biodiversity conservation to invest in LSAs that can be sustained and involve the least costs to local communities. Primary production of the forests, a proxy for a functional habitat, continued to decrease. Preventing communities from locating closer to forests could improve fruit bat diversity, which contributes to natural forest regeneration. Improving conservation attitudes should be an objective of conservation at the landscape scale. On the basis of the results, I developed a conceptual model for forest biodiversity conservation in a biocomplexity framework. This model could be useful for evaluating conservation in tropical forest areas. Lessons from this study can be applied in other incentive-based conservation programs such as payments for ecosystem services systems and carbon market schemes. I suggest that this study be repeated after a decade and that other socio-political and biogeochemical variables be integrated into future studies.

Critical to our understanding of wildlife diseases is the recovery phase, a period during which individuals clear infections and return to normal patterns of behavior and physiology. Most research on effects of white nose syndrome (WNS), an emerging fungal disease in bats, has focused on the pathophysiology of winter mortality and the effects of WNS on hibernating populations. The period immediately following emergence from hibernation has received little attention, but is a critically important time for survivors of the disease. During this time, survivors face significant physical and physiological challenges as they migrate to summer habitats, potentially begin gestation in the case of reproductive females, and begin to recover from wing damage caused by the fungus, which can be extensive and may greatly increase the energetic cost of flight. In this study, I (1) test the hypothesis that free-ranging bats heal from WNS-induced wing damage, (2) determine how WNS-induced wing damage changes skin surface lipid profiles on free-ranging bats, and (3) describe the temporal process of disease recovery in a colony of captive bats, including analyses of body mass, wing damage, pathogen load, skin surface lipid profiles, and histopathological metrics of WNS. I find that bats can quickly heal from wing damage in the wild and appear healthy as early as mid-July in New England. Analysis of skin surface lipids does not reveal any striking differences between bats with wing damage and those without, although there are trends towards lower total surface lipids and increased levels of cutaneous cholesterol in bats with severe wing damage. Finally, I show that within 40 days of emerging from hibernation, bats quickly clear the fungal infection and gain body mass, undergoing rapid healing of wing damage and changes in skin surface lipid composition. Bats depend on their wings for a variety of vital processes including physiological regulation, locomotion and feeding. To fully understand the consequences of WNS and develop actionable management strategies, it is important to consider the long-term effects of this disease. My study helps fill critical knowledge gaps and will aid in the future conservation and management of affected bat species.

The glenohumeral (GH) joint is the most mobile joint in the human body, but its mobility inherently increases the risk of instability. The humeral head sits in a shallow glenoid in the scapula like a golf ball sitting on a tee. The stability in this joint is provided by the rotator cuff muscles and tendons that actively pull the humerus back into the socket to prevent dislocation, especially during overhead motions. However, the rotator cuff is prone to tears, resulting in pain, loss of mobility, and recreational limitations. Surgical reattachment of the tendon to the bone is challenging due to the mechanical disparity between the two tissues, resulting in stress concentrations and a high risk of retear. Notably, the specialized tissue at the tendon-to-bone attachment, which facilitates stress transfer between tendon and bone in healthy joints, does not regenerate after surgical reattachment and healing, making tendon-to-bone repairs prone to re-tears. A comprehensive understanding of GH joint biomechanics is essential for developing early interventions to prevent rotator cuff injuries. Furthermore, improving tendon-to-bone fixation during rotator cuff repair is critical to improve post-surgery outcomes. In the last decade, bioinspired solutions have shown considerable promise for addressing several biomedical problems. This thesis draws bioinspiration from two animals that have evolved unique mechanical functions: (i) the bat shoulder joint, which facilitates repetitive overhead motions during flight and may offer insights into rotator cuff pathology and (ii) the curvature of python snake teeth, which enables secure grasping of prey without soft tissue tearing. In the first part of the thesis, the bat shoulder was studied for its unique characteristics relative to mice. Overhead motions in humans often lead to shoulder injuries, partly because the bony anatomy of the unstable GH joint places greater stress on the joint's surrounding soft tissues to stabilize these motions. Traditional animal models used to study shoulder pathology are quadrupeds, which lack the capacity for overhead motion. In contrast, bats consistently engage in overhead motion during flight, subjecting their shoulders to substantial loading throughout their relatively long lifespan. Remarkably, the biomechanical demands placed on a bat's shoulder are estimated to exceed those of a competitive swimmer’s by 45-fold, despite sharing similar coracoacromial arch anatomy with humans. We were inspired to study functional adaptations in the shoulders of bats that enable this overhead motion. We performed comparative anatomy studies of the shoulders of bats and mice, similarly-sized quadrupeds. By quantifying the constraints imposed by the bony anatomy, we identified adaptations of the shoulder, including the rotator cuff tendons, that allow bats to sustain overhead motion in a high stress, repeated loading environment, without injury. In the second part of the thesis, python teeth were used as inspiration to develop a repair device optimized to grasp the rotator cuff without tearing. Rotator cuff repair surgeries fail frequently, with 20-94% of the 600,000 repairs performed annually in the United States resulting in retearing of the rotator cuff. The most common cause of failure is sutures tearing through tendons at grasping points. To address this issue, we examined the specialized teeth of snakes of the Pythonoidea superfamily, which effectively grasp soft tissues without tearing. To apply this non-damaging and effective gripping approach to the surgical repair of tendons, we developed and optimized a python-tooth inspired array as an adjunct to current rotator cuff suture repair, and found that it nearly doubled repair strength. Integrated simulations, 3D printing, and ex vivo experiments revealed a relationship between tooth shape and grasping mechanics, and enabled optimization of a tooth array device to enhance rotator cuff repair to distribute stresses and increase tendon-bone contact. The efficacy of the approach was demonstrated via human cadaver tests, suggesting an alternative to traditional suturing paradigms that may reduce tendon re-tearing. Collectively, these studies contribute to a better understanding of the biomechanics of the GH joint and offer novel, bioinspired approaches for rotator cuff repair. The functional adaptations of bats provide insight into developing new approaches to treat GH joint instability, and a clinically relevant python-tooth inspired device can ultimately reduce the high rates of re-rupture currently observed in rotator cuff repair.

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