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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Aspectos epidemiol?gicos da Babesia canis vogeli em c?es da Baixada Fluminense, RJ / Aspects of Babesia canis vogeli in dogs from Baixada Fluminense, RJ

VILELA, Joice Aparecida Rezende 30 March 2012 (has links)
Submitted by Jorge Silva (jorgelmsilva@ufrrj.br) on 2017-05-05T17:41:38Z No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) / Made available in DSpace on 2017-05-05T17:41:38Z (GMT). No. of bitstreams: 1 2012 - Joice Aparecida Rezende Vilela.pdf: 2330778 bytes, checksum: 444dfb2be034c128fc34d8e5ad64d910 (MD5) Previous issue date: 2012-03-30 / CAPES / The canine babesiosis, is a disease widely distributed in Brazil and worldwide, caused by? protozoa of the genus Babesia?spp. that parasitize erythrocytes and it?s transmitted by ticks? Ixodidae. This study aimed to characterize the epidemiology of canine babesiosis through? questionnaires and serological diagnosis by ELISA, diagnosis of Babesia? spp. infection in? dogs by real?time PCR technique, identify the subspecies of B. canis?by RFLP, characterize? the hematological aspects, diagnosis by direct microscopy, identification of ectoparasites and? diagnosis by real?time PCR, the agent of canine babesiosis in ticks from dogs from rural and? urban areas from Serop?dica city, RJ State. The present study evaluated 311 dogs, 167? (n=53,7%) of rural and 144 (n=46,3%) in urban areas. By ELISA technique, was found 39? dogs (12,54%) serologically positive for Babesia canis, with frequency of seropositivity of? 21,56% ???(n=36/167) in dogs of rural area and 2,08% (n=3/144) in dogs from urban area, and? therefore the rural area, the highest seropositivity (p<0,0001). The variables associated with? seropositivity were: area of residence of the dog, breed, age, presence of ticks, environment of? dog access, contact with other animals, the animal's habits, condition of cleanliness of the? environment, absence of veterinary care and animal wandering. The profile of the owner and? its management were striking features in parasitism by ticks and consequently the frequency? of seropositivity to the agent. The diagnostic by PCR real??time of blood samples from dogs? showed a frequency of 11,90% (n=37/311). The subspecies identified affecting dogs of this? region was B. canis vogeli. Of the total of 311 dogs evaluated, infestations by ectoparasites? were found in 252 (81,03%) dogs at the time of collection. Of these, 70,64% (n=178/252)? were infested with ticks. In relation to the infestation, Rhipicephalus sanguineus?was found in? 68,54% (n=122/178) of the dogs, Amblyomma cajennense?in 11,80% (n=21/178), A. ovale?in? 3,37% (n=6/178) and A. dubitatum? in 0,56% (n=1/178). Nymphs of R. sanguineus? and? Amblyomma sp. were observed in 13,48% (n=24/178) and 5,62% (n=10/178) of dogs infected? with ticks, respectively. The tick species most common in urban and rural areas was R. sanguineus, which was associated with positivity of B. canis?in dogs by molecular method.? The frequency of ticks positive for B. canis?was 5% (n=12/240). The DNA from B. canis?was? detected in nine R. sanguineus?ticks, an A. cajennense?adult and two nymphs of Amblyomma? sp. The main hematological changes in infected dogs were: anemia, thrombocytopenia and? hemoglobinemia. The study concluded that canine babesiosis caused by B. canis vogeli?occurs? in rural and urban areas from the Serop?dica being of great importance its occurrence in the? cities of the Rio de Janeiro State, which have characteristics similar expansion of urbanization? to rural areas with little distinction between the two areas and points to the importance of? diagnosis and identification of the pathogen and vectors in dogs, in order to know the? epidemiological conditions for the adoption of appropriate preventive measures, because in? addition to affect the animal health, many of these vectors are capable to transmit pathogens? to humans.? / A babesiose canina, patologia de ampla distribui??o no Brasil e no mundo, ? causada por? protozo?rios do g?nero Babesia? spp., que parasitam hem?cias e s?o transmitidos por? carrapatos Ixodidae. O presente trabalho objetivou determinar a epidemiologia da babesiose? canina atrav?s de aplica??o de question?rios e diagn?stico por ELISA, diagn?stico por? microscopia direta, diagn?stico de Babesia canis? por meio da PCR em tempo real,? identifica??o das subesp?cies de B. canis? por meio da RFLP, caracteriza??o das principais? altera??es hematol?gicas, identifica??o dos ectoparasitos e diagn?stico atrav?s da PCR em? tempo real do agente da babesiose canina em carrapatos de c?es do munic?pio de Serop?dica,? Baixada Fluminense ??RJ. No presente estudo foram avaliados 311 c?es, sendo 167 (n=53,7%)? de ?rea rural e 144 (n=46,3%) de ?rea urbana. Atrav?s do teste de ELISA, encontrou?se um? total de 39 c?es (12,54%) sorologicamente positivos para Babesia canis, com frequ?ncia de? 21,56% (n=36/167) nos c?es de ?rea rural e 2,08% (n=3/144) nos c?es de ?rea urbana, sendo,? portanto a ?rea rural, a de maior soropositividade (p<0,0001). As vari?veis que apresentaram? associa??o com a soropositividade foram: ?rea de domic?lio do c?o, defini??o racial, idade,? infesta??o por carrapatos, locais de acesso do c?o, contato com outros animais, h?bito do? animal, ambiente do c?o, aus?ncia de limpeza do ambiente, aus?ncia de assist?ncia? veterin?ria, aus?ncia de abrigo e origem errante. O perfil do propriet?rio e o manejo do c?o? apresentaram caracter?sticas marcantes no parasitismo por carrapatos e consequentemente na? frequ?ncia de soropositividade para o agente. O diagn?stico pela PCR em tempo real de? amostras sangu?neas de c?es revelou uma frequ?ncia de 11,90% (n=37/311). A subesp?cie? identificada acometendo c?es desta regi?o foi B. canis vogeli. Do total de 311 c?es avaliados,? foram observadas infesta??es por ectoparasitos em 252 (81,03%) c?es. Destes, 70,64%? (n=178/252) apresentaram?se infestados por carrapatos. Em rela??o ? infesta??o por? carrapatos, Rhipicephalus sanguineus foi encontrado em 68,54% (n=122/178) dos c?es,? Amblyomma cajennense em 11,80% (n=21/178), A. ovale em 3,37% (n=6/178) e A. dubitatum em 0,56% (n=1/178) dos c?es infestados por carrapatos. Ninfas de R. sanguineus e? Amblyomma sp. foram observadas em 13,48% (n=24/178) e 5,62% (n=10/178) dos c?es? infestados por carrapatos, respectivamente. A esp?cie de carrapato mais frequente nos meios? urbano e rural foi R. sanguineus, que apresentou associa??o com a positividade de B. canis? vogeli?nos c?es pelo m?todo molecular. A frequ?ncia de carrapatos positivos para B. canis foi? de 5% (n=12/240). O DNA de B. canis foi detectado em nove carrapatos adultos de R. sanguineus, em um adulto de A. cajennense? e em duas ninfas de Amblyomma? sp.. As? principais altera??es hematol?gicas nos c?es infectados foram: anemia, hemoglobinemia e? trombocitopenia. O estudo permitiu concluir que a babesiose canina causada por B. canis vogeli?ocorre nas ?reas rurais e urbanas do munic?pio estudado sendo de grande import?ncia a? sua ocorr?ncia nos munic?pios da Baixada Fluminense do Estado do Rio de Janeiro, os quais? apresentam caracter?sticas semelhantes de expans?o da urbaniza??o para o meio rural com? pequena distin??o entre as duas ?reas e aponta para a import?ncia do diagn?stico do pat?geno? e identifica??o de vetores nos c?es, visando o conhecimento das condi??es epidemiol?gicas? para a ado??o de medidas profil?ticas adequadas, pois al?m de afetarem a sanidade animal? muitos destes vetores s?o capazes de veicularem pat?genos transmiss?veis ao homem.??

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