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Laser de diodo (810nm) na reducao bacteriana periodontal em pacientes cardiovasculares (coronariopatas)JACOB, TANIA D.T.L. 09 October 2014 (has links)
Made available in DSpace on 2014-10-09T12:52:55Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:02:01Z (GMT). No. of bitstreams: 0 / Dissertacao (Mestrado Profissionalizante em Lasers em Odontologia) / IPEN/D-MPLO / Intituto de Pesquisas Energeticas e Nucleares, IPEN/CNEN-SP; Faculdade de Odontologia, Universidade de Sao Paulo
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An investigation of certain haematological parameters of the rainbow trout, Salmo gairdneri Richardson with reference to the possible effects of bacterial infectionBarham, William Theodore 10 February 2014 (has links)
M.Sc. / Please refer to full text to view abstract
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Comparison of Xanthomonas campestris pv. mangiferaeindicae from mango production areas in South AfricaGantsho, Nomphelo 20 February 2006 (has links)
Please read the abstract in the dissertation. Copyright / Dissertation (MSc (Plant Pathology))--University of Pretoria, 2007. / Microbiology and Plant Pathology / unrestricted
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Prevalence of Escherichia coli O157:H7 in water and meat and meat products and vegetables sold in the Eastern Cape Province of South Africa and its impact on the diarrhoeic conditions of HIV/AIDS patientsAbong'o, Benard Omondi January 2008 (has links)
Water and food borne Escherichia coli O157:H7 could be one of the pathogens posing high health risk to patients suffering from Acquired Immunodeficiency Syndrome (AIDS) because of its incrimination in diarrhoea cases in AIDS patients. The present study, which was conducted between March 2005 and August 2006, investigated the prevalence of E. coli O157:H7 in water, meat and meat products and vegetables and its impact on diarrhoeic conditions of confirmed and non-confirmed HIV/AIDS patients in the Amathole District in the Eastern Cape Province of South Africa. The water samples used in the study were obtained from stand pipes supplying treated drinking water to communities residing in Fort Beaufort, Alice, Dimbaza and Mdantsane whereas borehole waters were sampled from Ngwenya and Kwasaki. The meat and meat products and vegetable samples were purchased from shops, butcheries, supermarkets and open air markets in Fort Beaufort, Alice and Mdantsane. The stool swabs used in the study were obtained from HIV/AIDS and outpatient clinics at Frere Hospital in East London. A total of 180 each of water, meat and meat products and vegetable samples and another 360 stool samples were analyzed for E. coli O157:H7. Presumptive E. coli O157 was isolated from the samples by culture-based methods and confirmed using Polymerase Chain Reaction techniques. Anti-biogram as well as risk assessment were also carried out using standard methods. The viable counts of presumptive E. coli O157 for water samples ranged between 3.3 × 104 and 1.71 × 105 CFU/ml, and between 1.8 × 104 and 5.04 × 106 CFU/g for meat and meat products, whereas those for vegetables ranged between 1.3 × 103 and 1.6 × 106 CFU/g. The counts of presumptive E. coli O157 for the water and vegetable samples were not significantly different whereas those for meat and meat products were found to be significantly different (P ≤ 0.05). The prevalence rates of presumptive E coli O157 in meat and meat products was 35.55 percent (64/180), and 25.55 percent (46/180) and 21.66 percent (39/180) for water and vegetables respectively. Prevalence of presumptive E. coli O157 in the stool samples of HIV/AIDS patients was 36.39 percent (131/360), of which 56.5 percent (74/131) and 43.5 percent (57/131) were from stools of confirmed and non-confirmed HIV/AIDS patients, respectively. Molecular analysis of representative presumptive E. coli O157 indicated that 10.29 percent (4/39) of vegetables; 14.81 percent (4/27) of water and 38.46 percent (5/13) of meat and meat products carried E. coli O157:H7. Also 36 percent (9/25) and 17.24 percent (5/29) of the stool samples were positive for E. coli O157:H7. Antimicrobial susceptibility profile revealed that all of the E. coli O157:H7 isolated from water, meat and meat products and vegetables as well as those isolated from stools of confirmed and non-confirmed HIV/AIDS patients were resistant (R) to gentamycin and erythromycin. However, 75 percent (20/27) of these isolates were resistant (R) to ampicillin and tetracycline whereas approximately 25 percent (6/27) were resistant (R) to nalidixic acid, ceftriaxone, and chloramphenicol. All the isolates (27/27) were susceptible (S) to amikacin. Probability of risk of E. coli O157:H7 infection was high for confirmed HIV/AIDS patients than for the non-confirmed HIV/AIDS patients. Estimated probability of risk of E. coli O157:H7 due to ingestion of water was 1.00 for 100 confirmed and non-confirmed HIV/AIDS patients. Risk due to meat and meat products was estimated at 0.27 and 0.20 and for vegetables at 0.21 and 0.15 per 100 confirmed and non-confirmed HIV/AIDS patients. The findings of this study predicted a possible link between E. coli O157:H7 isolated from drinking water, meat and meat products and vegetables and diarrhoeic conditions in both confirmed and non-confirmed HIV/AIDS patients, and concludes that confirmed HIV/AIDS patients can be at higher risk of contracting water and food borne E. coli O157:H7 than nonconfirmed HIV/AIDS patients. It is thus recommended that proper water treatment and food handling, maximum food and water safety and sanitation as well as personal body hygiene should be maintained, in order to prevent E. coli O157:H7 infections. Education initiatives and active surveillance of E. coli O157:H7 should be taken by all the stake-holders working directly or indirectly towards ensuring enduring sound public health.
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Investigation of antibacterial compounds present in Combretum woodii duemmerFamakin, James Olusanya 28 June 2007 (has links)
Please read the abstract in the section 00front of this document / Dissertation (MSc (Pharmacology))--University of Pretoria, 2007. / Pharmacology / unrestricted
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Bacterial diseases of dry beans in South Africa with special reference to common bacterial blight and its controlFourie, Deidre 30 June 2005 (has links)
Please read the abstract in the section 00front of this document / Thesis (PhD (Microbiology))--University of Pretoria, 2005. / Microbiology and Plant Pathology / unrestricted
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Synthesis of 1, 3, 5 - Triazine Based Antimalarial DrugsMugwena, Dakalo Sandra 21 September 2018 (has links)
MSc (Chemistry) / Department of Chemistry / This dissertation focuses on the application of 1, 3, 5-triazine in a pharmaceutical point of view since it has wide range of uses as described in chapter 1. Malaria is one of the most prevalent and deadly infectious diseases worldwide though there are already many synthesized anti-malarial drugs which are in use presently, drug resistance seems to be one of the major problem and combination therapy seems to be the only solution for now.
Hence in this study we used hybridization as a tool (Figure 9) to synthesize new antimalarial drugs using 1, 3, 5-triazine as an intermediate linker, linking known anti-malarial drug, different amine and chloroquine-like amines together using nucleophilic substitution reaction. As explained in chapter four of this dissertation, triazine is used to synthesize mono-, di- and tri-amino-1, 3, 5-triazine substituted products. Using THF as a solvent and K2CO3 as a base changing in temperatures, from 0 oC 25 oC or reflux. Some products were synthesized using microwave irradiation.
The application of triazine as an intermediate linker in the above mentioned condition yielded five mono-amino substituted dichloro-1, 3, 5-triazine (21-25) in an average yield of 82%, three amino substitution chloro-1, 3, 5-triazine (26-28) in an average yield of 87%, two amino substituted-1, 3, 5-triazine (29, 30) in an average of 90%, nine chloroquine-like synthesized compounds (33-41) in 84 % average yields respectively. / NRF
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A study of biochemical and morphological aspects of macrophage function in experimental murine Nocardia asteroides and Nocardia brasiliensis infectionsStephens, Janet January 1987 (has links)
It is submitted in this thesis that the degree of activation or inhibition of macrophage function may differ in N. asteroides and N. brasiliensis infections with respect to release of plasminogen activator and of lysozyme The pattern of secretion of plasminogen activator and lysozyme in N. asteroides infections appears to differ in N. brasiliensis infection; and there is possibly a difference in the amount of lysozyme released by 2 day N. asteroides-activated macrophages and 2 day N. brasiliensis -activated macrophages. Strains of Nocardia organism did not influence macrophage morphology or ultrastructure. The study also shows the biochemical characteristics of plasminogen activator and lysozyme release, but not macrophage morphology and ultrastructure, are modified in the first 21 days of experimental Nocardia infections. There are three apparent mechanisms by which virulent strains of N. asteroides manage to survive within macrophages: (i) an ability to inhibit phagosome-lysozome fusion: (ii) alteration in the intraphagosomal pH: and (iii) alteration in the activity of the lysozomal enzyme acid-phosphatase. This study attempted to elucidate further the mechanisms enabling Nocardia organisms to persist and grow within macrophages. Reduced lysozyme release reflects diminished functional status of the macrophages of mice inoculated with N. asteroides or N. brasiliensis at certain times during infection. Reduced intracellular lysozyme levels have been linked with defects in bactericidal function. Such a reduction in intracellular and consequently extracellular levels of lysozyme might explain the capacity of Nocardia to survive intracellularly and to proliferate in the macrophage host.
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Molecular mechanisms of pathogenesis incited by Erwinia carotovora subsp. carotovoraRoberts, Daniel Paul January 1985 (has links)
Erwinia carotovora subsp. Carotovora (Ecc) incites soft-rot on many plants. It is believed that soft-rot is due to the concerted activity of extracellular enzymes. Recombinant DNA techniques were used to study the molecular basis of pathogenesis incited by Ecc. Specifically, a clone library of Ecc strain EC14 DNA in plasmid pBR322 was constructed and transformed into Escherichia coli strain HB101. Some of the E. coli strains that contain these hybrid plasmids produce pectinases or cellulase(s). Plasmid pDR1 contains a 3.4 kilobase (kb) EC14 DNA fragment and mediates the production of endo-pectate lyases with isoelectric points (pI) of 9.5 and 7.5 in strain HB101. The pI 9.5 enzyme is believed to be the major extracellular pectolytic enzyme in soft-rot while the pI 7.5 enzyme has no documented counterpart in EC14. Subclone and transposon tn5 analyses of pDR1 indicate that 1.5 kb is necessary for the production of the pI 9.5 and pI 7.5 enzymes and that these enzymes are produced independently of other EC14 pectate lyase enzymes. Plasmid pDR30 contains a 2.1 kb EC14 DNA insert that mediates the production of an endo-polygalacturonase and an exo-pectate lyase in HB101. The exo-pectate lyase encoded by pDR30 produces an inducer of endo-pectate lyase synthesis as a reaction product. The endo-polygalacturonase encoded by pDR30 is thought to play a role in plant cell wall pectic polymer degradation. Restriction endonuclease and Southern hybrididizatian analyses indicate that the EC14 genes on plasmids pDR1 and pDR30 are not part of the same operon. Escherichia coli strain HB101 containing plasmid pDR1 or plasmid pDR30 is unable to macerate potato tuber slices. However, HB101 containing plasmids pDR1 and pDR30 can cause limited maceration of potato tuber slices. There appears to be a genetic interaction between plasmids pDR1 and pDR30 in maceration of potato tuber tissue. However, the EC14 gene(s) contained on plasmid pDR1 are transcribed independently of the EC14 genes contained on plasmid pDR30. It is possible that transcription of certain pectolytic enzymes independent of other pectolytic enzymes provides a flexible system for plant cell wall pectic polymer degradation. / Ph. D.
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Control of bacterial pathogens associated with mastitis in dairy cows with natural antimicrobial peptides produced by lactic acid bacteriaPieterse, Renee 03 1900 (has links)
Thesis (MSc (Microbiology))--Stellenbosch University, 2008. / Mastitis is considered to be the most costly disease affecting the dairy industry.
Management strategies involve the extensive use of antibiotics to treat and prevent this
disease. Prophylactic dosages of antibiotics used in mastitis control programmes could select
for strains with resistance to antibiotics. In addition, a strong drive towards reducing
antibiotic residues in animal food products has lead to research in finding alternative
antimicrobial agents.
Streptococcus macedonicus ST91KM, isolated from bulgarian goat yoghurt, produces
the bacteriocin macedocin ST91KM with a narrow spectrum of activity against Grampositive
bacteria. These include mastitis pathogens Streptococcus agalactiae, Streptococcus
dysgalactiae, Streptococcus uberis, Staphylococcus aureus and Staphylococcus epidermidis
as well as Lactobacillus sakei and Micrococcus varians. Macedocin ST91KM is, according
to tricine-SDS PAGE, between 2.0 and 2.5 kDa in size. The activity of macedocin ST91KM
remained unchanged after 2 h of incubation at pH 2.0 to 10.0 and 100 min at 100 °C. The
peptide was inactivated after 20 min at 121 °C and when treated with pronase, pepsin and
trypsin. Treatment with α-amylase had no effect on activity, suggesting that the mode of
action does not depend on glycosylation. Precipitation with 60 % saturated ammonium
sulphate, followed by Sep-Pak C18 separation recovered 43 % of macedocin ST91KM.
Amplification of the genome of strain ST91KM with primers designed from the sequence of
the macedocin prescursor gene (mcdA) produced two fragments (approximately 375 and 220
bp) instead of one fragment of 150 bp recorded for macedocin produced by S. macedonicus
ACA-DC 198. Strain ACA-DC 198 was not available. However, the DNA fragment
amplified from strain LMG 18488 (ACA-DC 206), genetically closely related to strain ACADC
198, revealed 99 % homology to the mcdA of S. macedonicus ACA-DC 198 (accession number DQ835394). Macedocin ST91KM may thus be a related bacteriocin described for S.
macedonicus.
The peptide adsorbed equally well (66 %) to L. sakei LMG13558 and insensitive
cells, e.g. Enterococcus faecalis BFE 1071 and FAIR E92, and Streptococcus caprinus
ATCC 700066. Optimal adsorption of macedocin ST91KM was recorded at 37 °C and 45 °C
and at pH of 8 - 10. Addition of solvents decreased adsorption by 50%, suggesting that the
receptors to which the bacteriocin binds have lipid moieties. The addition of MgCl2, KI and
Na2CO3 completely prevented adsorption of macedocin ST91KM to the target cells, possibly
due to competitive ion adsorption on the bacterial cell surface. The peptide has a
bacteriocidal mode of action, resulting in lysis and the release of DNA and β-galactosidase.
Atomic force microscopy of sensitive cells treated with macedocin ST91KM have shown
deformation of the cell structure and developing of irregular surface areas.
Antimicrobial susceptibility patterns were evaluated against eighteen mastitis
pathogens. All isolates tested were resistant to methicillin and oxacillin, but had minimum
inhibitory concentrations (MICs) falling in the intermediate and susceptible range against
erythromycin. S. agalactiae and S. epidermidis had the highest sensitivity to macedocin
ST91KM. A teat seal preparation containing macedocin ST91KM effectively released
bacteriocin inhibiting the growth of the bacterial pathogen. Macedocin ST91KM could form
the basis for an alternative dry cow therapy to prevent mastitis infections in dairy cows, as it
is effective against pathogens that display resistance to conventional antibiotic therapy.
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