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Investigation of some virulence properties of enteropathogenic Escherichia coliLaw, D. January 1989 (has links)
No description available.
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Epidemiology and evolution of pneumococcal neuraminidasesKing, Samantha Jane January 1999 (has links)
No description available.
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Expression of Shiga toxin genes in Escherichia coliKimmitt, Patrick Thomas January 1999 (has links)
No description available.
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Regulation of virulence and antimicrobial peptide resistance in Pseudomonas aeruginosaGooderham, William James 11 1900 (has links)
Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium that is also a major opportunistic human pathogen in nosocomial infections and cystic fibrosis chronic lung infections. These P. aeruginosa infections can be extremely difficult to treat due to the high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated. It was demonstrated here that the psrA gene, encoding a transcriptional regulator, was up-regulated in response to sub-inhibitory concentrations of antimicrobial peptides. Compared to wild-type and the complemented mutant, a P. aeruginosa PAO1 psrA::Tn5 mutant displayed intrinsic super-susceptibility to polymyxin B, a last resort antimicrobial used against multi-drug resistant infections, and indolicidin, a bovine neutrophil antimicrobial peptide; this super-susceptibility phenotype correlated with increased outer membrane permeability. The psrA mutant was also defective in simple biofilm formation, rapid attachment, and normal swarming motility, phenotypes that could be complemented by the cloned psrA gene. The role of PsrA in global gene regulation was studied by comparing the psrA mutant to wild-type by microarray analysis, demonstrating that 178 genes were up or down-regulated by greater than 2-fold (P ≤0.05). Dysregulated genes included those encoding known PsrA targets, the type III secretion apparatus and effectors, adhesion and motility genes and a variety of metabolic, energy metabolism and outer membrane permeability genes. This indicates that PsrA is a central regulator of antimicrobial peptide resistance and virulence. P. aeruginosa containing a mutation in the PhoQ sensor kinase-encoding gene was highly attenuated for persistence in a rat chronic lung infection model. In addition, the polymyxin B hyper-resistant phoQ mutant displayed reduced type IV pili-dependent twitching motility and was less cytotoxic towards human bronchial epithelial cells, indicating that the virulence defect observed could be due at least in part to these phenotypes. Using microarrays it was further demonstrated that PhoQ regulates a large number of genes that are PhoP-independent and that the phoQ mutation leads to up-regulation of PhoP- and PmrA regulated genes as well as other genes consistent with its virulence phenotypes.
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Regulation of virulence and antimicrobial peptide resistance in Pseudomonas aeruginosaGooderham, William James 11 1900 (has links)
Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium that is also a major opportunistic human pathogen in nosocomial infections and cystic fibrosis chronic lung infections. These P. aeruginosa infections can be extremely difficult to treat due to the high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated. It was demonstrated here that the psrA gene, encoding a transcriptional regulator, was up-regulated in response to sub-inhibitory concentrations of antimicrobial peptides. Compared to wild-type and the complemented mutant, a P. aeruginosa PAO1 psrA::Tn5 mutant displayed intrinsic super-susceptibility to polymyxin B, a last resort antimicrobial used against multi-drug resistant infections, and indolicidin, a bovine neutrophil antimicrobial peptide; this super-susceptibility phenotype correlated with increased outer membrane permeability. The psrA mutant was also defective in simple biofilm formation, rapid attachment, and normal swarming motility, phenotypes that could be complemented by the cloned psrA gene. The role of PsrA in global gene regulation was studied by comparing the psrA mutant to wild-type by microarray analysis, demonstrating that 178 genes were up or down-regulated by greater than 2-fold (P ≤0.05). Dysregulated genes included those encoding known PsrA targets, the type III secretion apparatus and effectors, adhesion and motility genes and a variety of metabolic, energy metabolism and outer membrane permeability genes. This indicates that PsrA is a central regulator of antimicrobial peptide resistance and virulence. P. aeruginosa containing a mutation in the PhoQ sensor kinase-encoding gene was highly attenuated for persistence in a rat chronic lung infection model. In addition, the polymyxin B hyper-resistant phoQ mutant displayed reduced type IV pili-dependent twitching motility and was less cytotoxic towards human bronchial epithelial cells, indicating that the virulence defect observed could be due at least in part to these phenotypes. Using microarrays it was further demonstrated that PhoQ regulates a large number of genes that are PhoP-independent and that the phoQ mutation leads to up-regulation of PhoP- and PmrA regulated genes as well as other genes consistent with its virulence phenotypes.
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Regulation of virulence and antimicrobial peptide resistance in Pseudomonas aeruginosaGooderham, William James 11 1900 (has links)
Pseudomonas aeruginosa is a ubiquitous environmental Gram-negative bacterium that is also a major opportunistic human pathogen in nosocomial infections and cystic fibrosis chronic lung infections. These P. aeruginosa infections can be extremely difficult to treat due to the high intrinsic antibiotic resistance and broad repertoire of virulence factors, both of which are highly regulated. It was demonstrated here that the psrA gene, encoding a transcriptional regulator, was up-regulated in response to sub-inhibitory concentrations of antimicrobial peptides. Compared to wild-type and the complemented mutant, a P. aeruginosa PAO1 psrA / Science, Faculty of / Microbiology and Immunology, Department of / Graduate
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Interplay between bacterial virulence and plant innate immunity in Ppseudomonas-arabidopsis interactionsLi, Xinyan January 1900 (has links)
Doctor of Philosophy / Department of Plant Pathology / Jianmin Zhou / Plants activate innate immune responses or innate immunity upon pathogen infection. There are two types of plant innate immunity: PAMP-triggered innate immunity (PTI) and effector-triggered innate immunity (ETI). The molecular basis for ETI has been well documented. However, the study on PTI and its interplay with pathogen virulence is in its infancy. My research focuses on the interplay between PTI and bacterial virulence in Pseudomonas-Arabidopsis interactions.
NHO1, a gene required for nonhost resistance to Pseudomonas syringae, encodes for the 3-glycerol kinase in Arabidopsis genome. NHO1 functions, at least in part, by depriving glycerol from nonhost bacteria cells. NHO1 is induced by a well-known bacteria PAMP flg22. The induction of NHO1 correlates well with the resistance against Pseudomonas syringae pv. tabaci because a mutant strain of P. s. pv. tabaci deficient in NHO1 induction gains partial virulence on Arabidopsis plants. P. s. pv. tomato strain DC3000 induces transient NHO1 expression that is suppressed in a type III secrection system-dependent manner. Using protoplast assay, nine DC3000 effectors that are able to suppress NHO1 were identified. One of them, HopAI1, induces leaf chlorosis and helps nonpathogenic bacterial growth when expressed in Arabidopsis plants, suggesting that HopAI1 has virulence activity in planta.
To study AvrB virulence activity in Arabidopsis plants, one mutant compromised in AvrB-specific RAR2.6 induction has been characterized in detail. rrb3 is more susceptible to a nonhost bacteria P. s. pv. tabaci strain 6505, a virulent bacteria P. s. pv. tomato strain DC3000 and an avirulent bacteria strain DC3000 (avrB). The mutant allele rrb3 carries a point mutation at the end of RAR1 CHORD II domain. RRB3 (RAR1), together with NDR1, is involved in the type II nonhost resistance to P. s. pv. tabaci but not in the type I nonhost resistance to P. s. pv. phaseolicola. RAR1 participates in basal resistance against DC3000 by antagonizing COI1 activity. AvrB targets RAR1 to trigger AvrB-dependent leaf chlorosis and enhanced bacterial growth. The AvrB-dependent enhanced bacterial growth but not leaf chlorosis requires COI1, suggesting that AvrB targets JA signaling pathway to promote parasitism.
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Mecanismos de captação de ferro por sideróforos em Chromobacterium violaceum / Mechanisms of iron uptake by siderophores in Chromobacterium violaceumBatista, Bianca Bontempi 03 September 2018 (has links)
A pouca solubilidade do ferro impõe desafios para sua captação por bactérias e outros organismos. Uma solução eficaz para este problema é a utilização de sideróforos próprios ou exógenos para solubilizar o ferro do ambiente ou de proteínas do hospedeiro e transportá-lo para o interior da célula bacteriana. Neste trabalho, identificamos vias de produção e captação de ferro por sideróforos e definimos o papel destas moléculas na virulência da bactéria Chromobacterium violaceum, um abundante componente da microbiota de solo e água que ocasionalmente causa graves infecções em humanos. Por meio de análises in silico, vários genes relacionados com a síntese e captação de sideróforos foram encontrados no genoma de C. violaceum ATCC 12472 em dois clusters de síntese de metabólitos secundários. Obtenção de linhagens mutantes de vários destes genes e caracterização destas linhagens por ensaios de CAS, curvas de crescimento em carência de ferro e ensaios de estimulação de crescimento revelaram que C. violaceum produz sideróforos endógenos. Essa produção mostrou-se dependente do percursor comum 2,3-DHBA produzido pelas enzimas codificadas pelos genes entCEBA (CV_1485-84-83-82) e de duas enzimas sintetases de peptídeo não ribossomais (NRPSs CV_1486 e CV_2233), as quais provavelmente montam dois sideróforos distintos do tipo catecolato. Cada sideróforo foi captado por um receptor dependente de TonB (RDTB) específico, com o sideróforo produzido via NRPS CV_1486 sendo captado pelo RDTB CV_1491, e o sideróforo produzido via NRPS CV_2233 sendo captado pelo RDTB CV_2230, uma vez que mutantes sem esses RDTBs acumularam sideróforos no meio externo no ensaio de CAS. Além de seus sideróforos endógenos, C. violaceum foi capaz de utilizar xenosideróforos do tipo catecolato de outras bactérias via o RDTB CV_1491. Ensaios de infecção em camundongos revelaram que tanto a síntese quanto a captação de seus sideróforos endógenos são importantes para a virulência de C. violaceum, pois as linhagens mutantes que não produzem sideróforos (?CV_1485-84- 83-82, ?CV_1485-84-83-82/1486::pNPT e ?CV_1486/2233::pNPT) ou são incapazes8 de captá-los (?CV_2230/1491) tiveram sua virulência diminuída em relação a linhagem selvagem. Os dados mostrando que o mutante que não capta ambos sideróforos de C. violaceum teve atenuação mais acentuada da virulência e induziu menor produção de NET em ensaios com neutrófilos in vitro sugerem que o acúmulo de sideróforos na infecção pode ser benéfico para o hospedeiro. Por fim, demonstramos a possibilidade de gerar mutantes de transposon em C. violaceum e ao realizarmos varredura de uma coleção destes mutantes identificamos ao menos um potencial novo fator de transcrição envolvido na regulação da síntese de sideróforo nesta bactéria. Portanto, os dados obtidos neste trabalho revelaram que C. violaceum utiliza-se de diferentes sideróforos endógenos para captação de ferro e que estas moléculas são importantes para seu estabelecimento no hospedeiro. / The low solubility of iron imposes challenges for its uptake by bacteria and other organisms. An effective solution to this problem is the use of own or exogenous siderophores to solubilize the iron from environmental or host sources and transport it into the bacterial cell. In this work, we identified pathways for production and uptake of siderophores, and we defined the role of these molecules in virulence of the bacterium Chromobacterium violaceum, an abundant component of the microbiota of soil and water, which occasionally causes serious infections in humans. By performing an in silico analysis, we found several genes related with synthesis and uptake of siderophores in the genome of C. violaceum ATCC 12472 within two secondary metabolite biosynthesis gene clusters. Obtaining mutant strains from several of these genes and characterizing these strains by CAS assays, growth curves under iron deficiency and growth stimulation assays revealed that C. violaceum produces endogenous siderophores. This production was shown to be dependent on the common precursor 2,3-DHBA produced by the enzymes encoded by the genes entCEBA (CV_1485-84-83-82) and on two non-ribosomal peptide synthetase enzymes (NRPSs CV_1486 and CV_2233), which probably build two distinct catecholate siderophores. Each siderophore was picked up by a specific TonB-dependent receptor (RDTB), with the siderophore produced via NRPS CV_1486 being picked up by RDTB CV_1491, and the siderophore produced via NRPS CV_2233 being picked up by RDTB CV_2230, since mutants without those RDTBs accumulated siderophores in the external environment in the CAS assays. In addition to its endogenous siderophores, C. violaceum was able to use catecholate-type xenosiderophores from other bacteria via the RDTB CV_1491. Infection assays in mice revealed that both the synthesis and the uptake of its endogenous siderophores are important for the virulence of C. violaceum, since mutant strains that do not produce siderophores (?CV_1485-84-83- 82, ?CV_1485-84-83- 82/1486 :: pNPT and ?CV_1486/2233 :: pNPT) or are unable to uptake them (?CV_2230/1491) had their virulence decreased relative to the wild type strain. The data showing that the mutant strain unable to uptake both siderophores of10 C. violaceum had more pronounced attenuation of virulence and induced lower NET production in in vitro neutrophil assays suggest that the accumulation of siderophores in the infection may be beneficial to the host. Finally, we demonstrated the possibility of generating transposon mutants in C. violaceum, and by screening a collection of these mutants we identified at least one potential novel transcription factor involved in the regulation of siderophore synthesis in this bacterium. Therefore, the data obtained in this work revealed that C. violaceum uses different endogenous siderophores for iron uptake and that these molecules are important for its establishment in the host.
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Caracterização funcional de fatores de transcrição da família MarR de Chromobacterium violaceum / Functional characterization of MarR family transcription factors in Chromobacterium violaceumMello, Maristela Previato 13 June 2018 (has links)
Os fatores de transcrição da família MarR atuam como sensores diretos de sinais intracelulares e regulam vários processos em bactérias, incluindo virulência e degradação de compostos aromáticos. Neste trabalho, identificamos de modo global os fatores de transcrição da família MarR envolvidos na virulência do patógeno oportunista de humanos Chromobacterium violaceum. Usando mutagênese por troca alélica, geramos mutantes nulos não polares para doze dos quinze reguladores da família MarR encontrados no genoma de C. violaceum. Em ensaios de virulência, quando injetados por via intraperitoneal em camundongos BALB/c, os mutantes ?CV_0210 (?ohrR), ?CV_0577 e ?CV_2726 foram menos virulentos, enquanto o mutante ?CV_1776 foi mais virulento, quando comparados à linhagem selvagem. Para os demais nove mutantes MarR não houve diferença na virulência. Para definir o regulon de alguns destes reguladores da família MarR, os perfis de expressão gênica foram determinados por ensaios de microarranjo de DNA e Northern blot para as linhagens mutantes ?CV_0210 (?ohrR), ?CV_1776, ?CV_1810 e ?CV_2726, para a linhagem selvagem superexpressando CV_2726 e para a linhagem selvagem em estresse oxidativo com hidroperóxido de cumeno (CHP). O regulon do repressor CV_1810 compreendeu dois operons divergentes, que codificam enzimas que possivelmente metabolizam compostos aromáticos, mas produtos do catabolismo destes compostos não funcionaram como ligantes capazes de antagonizar a repressão de CV_1810 no gene CV_1801. O regulon do ativador CV_2726, definido como quatorze genes comuns diferencialmente expressos em ensaios na ausência e na condição de superexpressão do gene CV_2726, revelou poucos genes (cstA) com potencial de estar envolvidos no fenótipo de menor virulência do mutante ?CV_2726. Os reguladores CV_0577 e CV_1776 foram alocados na subfamília UrtR de resposta a urato e provavelmente influenciam a virulência de C. violaceum com regulons sobrepostos. O regulon de CV_1776 abrangeu dezenas de genes, muitos deles relacionados ao catabolismo de aminoácidos, mas há poucos candidatos a fatores de 10 virulência clássicos (pecM, escU). Alguns genes do catabolismo/utilização de purinas (CV_0578 e CV_3771) foram regulados tanto por CV_1776 quanto por CV_0577 e responderam a presença de urato. O perfil transcricional da resposta adaptativa de C. violaceum a CHP, um ligante que oxida o regulador OhrR, revelou aumento na expressão de genes relacionados à detoxificação de peróxidos (enzimas antioxidantes e sistemas redutores de tiol), degradação da porção aromática do CHP (oxigenases) e proteção contra estresses secundários (reparo de DNA, choque térmico, limitação de ferro e nitrogênio). O regulon de OhrR revelou-se pequeno, incluindo dois genes com expressão aumentada, CV_0209 (ohrA) e CV_0208 (possível diguanilato ciclase), e três genes com expressão diminuída (hemolisina, quitinase e colagenase) no mutante ?ohrR. Assim, a virulência atenuada do mutante ?ohrR deve estar relacionada ao aumento da produção do segundo mensageiro cíclico di-GMP (c-diGMP) e à diminuição da expressão de enzimas degradativas extracelulares. Em conclusão, definimos a resposta transcricional à CHP, identificamos potenciais fatores de virulência, como a diguanilato ciclase, no regulon OhrR, e mostramos que C. violaceum utiliza os fatores de transcrição da família MarR CV_0577, CV_1776, CV_2726 e OhrR para modular sua virulência. / Transcription factors belonging to the MarR family act as direct intracellular sensors of signals and control many processes in bacteria, including virulence and degradation of aromatic compounds. In this work, we identify and characterize MarR family transcription factors controlling virulence in Chromobacterium violaceum, an opportunistic pathogen of humans. Using allelic exchange mutagenesis, we generate non-polar null mutants for twelve of the fifteen MarR family regulators found in the C. violaceum genome. In virulence tests, when introduced by intraperitoneal injection in BALB/c mice, the ?CV_0210 (?ohrR), ?CV_0577 and ?CV_2726 mutant strains were less virulent, while the ?CV_1776 was more virulent, when compared to the wild-type strain. The other nine MarR mutants showed no difference in virulence tests. To define the regulon of some MarR family transcription factors, the gene expression profiles were determined by DNA microarray analysis and Northern blot assays for the ?CV_0210 (?ohrR), ?CV_1776, ?CV_1810 and ?CV_2726 mutant strains, for the wild-type strain overexpressing CV_2726 and for the wild-type strain exposed to oxidative stress generated by cumene hydroperoxide (CHP). The CV_1810 is a repressor of a regulon that comprised two divergent operons encoding enzymes that possibly metabolize aromatic compounds, but catabolic products of these compounds did not function as ligands capable of antagonizing the repression of CV_1810 on the CV_1801 gene. The regulon of the activator CV_2726, defined as fourteen differentially expressed genes commonly found in assays in the absence and overexpression of the CV_2726 gene, revealed few genes (cstA) with potential to be involved in the phenotype of lower virulence of the ?CV_2726 mutant strain. Regulators CV_0577 and CV_1776 were allocated in the urate-responsive UrtR subfamily and probably afect the virulence of C. violaceum with overlapping regulons. The CV_1776 regulon contains dozens of genes, many of them related to amino acid catabolism, but there are few candidates for classical virulence factors (pecM, escU). Some genes related to catabolism/utilization of purine (CV_0578 and CV_3771) were 12 regulated by both CV_1776 and CV_0577 and responded to the presence of urate. The transcriptional profile of the adaptive response of C. violaceum to CHP, a ligand that oxidizes the OhrR regulator, revealed the upregulation of genes related to the detoxification of peroxides (antioxidant enzymes and thiol-reducing systems), degradation of the aromatic moiety of CHP (oxygenases), and protection against other secondary stresses (DNA repair, heat shock, iron limitation, and nitrogen starvation responses). The OhrR regulon was shown to be small, including two upregulated genes, CV_0209 (ohrA) and CV_0208 (putative diguanylate cyclase), and three downregulated genes (hemolysin, chitinase, and collagenase) in the ?ohrR mutant. Thus, the attenuated virulence of the ?ohrR mutant might be related to the increased production of the second messenger cyclic di-GMP (c-di-GMP) and the decreased expression of extracellular enzymes required for tissue dissemination, in this mutant strain. In conclusion, we have defined the transcriptional response to CHP, identified potential virulence factors such as diguanylate cyclase as members of the OhrR regulon, and shown that C. violaceum uses the transcription factors of the MarR family CV_0577, CV_1776, CV_2726 and OhrR to modulate its virulence.
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Capacidade de resistência à fagocitose e atividade bactericida de neutrófilos por distintas cepas de estafilococos associadas à mastite em vacas primíparas e multíparas / Ability to resist to phagocytosis and bacterial activity of neutrophils by distinct strains of staphylococci associated with mastitis in primiparous cowsSouza, Rodrigo Malzoni de 24 November 2017 (has links)
O grupo de estafilococos não-aureus (SNA), frequentemente isolados de quartos mamários com mastite subclínica, ápice do teto e ambiente, possue variabilidade ecológica que desafia a compreensão da patogenia a estes atribuída. Os fatores espécie-específicos associados à essa infecção ainda não foram identificados e a susceptibilidade difere entre vacas e quartos e promove diferentes perfis de infecção. Com o objetivo de avaliar a resistência à fagocitose e atividade microbicida, comparou-se a viabilidade, a produção intracelular de espécies reativas de oxigênio (ERO) e a fagocitose de neutrófilos sanguíneos de vacas primíparas e multíparas frente a distintos isolados viáveis de estafilococos. Utilizou-se doze vacas sadias (seis primíparas e seis multíparas) em terço médio de lactação e SO isolados viáveis de estafilococos (38 SNA e 12 Staphylococcus aureus) de diferentes nichos ecológicos. A viabilidade de neutrófilos (P = 0,55), produção de ERO (P = 0,12) e atividade funcional dos fagócitos (P = 0,33) foram semelhantes entre as primíparas e multíparas testadas . Contudo, foram observadas diferenças (P ≤0,05) entre os distintos grupos de espécies e estirpes de estafilococos quanto ao estímulo da produção intracelular de ERO pelos neutrófilos e à fagocitose. S. chromogenes de origens distintas, ápice do teto (P = 0,01), infecção intramamária transiente (P < 0,01) e infecção intramamárias persistente (P < 0,01) estimularam mais a produção de ERO pelos neutrófilos do que as outras espécies. Todos isolados foram fagocitados pelos neutrófilos, mas S. chromogenes resistiram mais eficientemente que as outras espécies de SNA, principalmente, S. chromogenes isolados do ápice do teto (P < 0,01). S. haemolyticus isolados do ápice do teta (P = 0,02) e infecção intramamária transiente (P < 0,01), assim como, S. fleurettii (P < 0,01), foram substancialmente fagocitados do mesmo modo que S. aureus isolado de suabe nasa\\ (P = 0,03). Mais evidente do que possíveis variações entre as respostas mamárias de primíparas e multíparas é a variação entre os SNA. Quanto mais adaptado à mama, maior resistência à fagocitose. / The group of non-aureus staphylococci (NAS), often isolated from mammary quarters with subclinical mastitis, teat apex and environment, has ecological variability that challenges the understanding of the pathogenesis attributed to them. The species-specific factors associated with this infection have not yet been identified and the susceptibility differs between cows and quarters and promotes different infection profiles. In order to evaluate the resistance to phagocytosis and I or microbicidal activity of these pathogens, the viability , intracellular production of reactive oxygen species (ROS) and blood neutrophil phagocytosis of primiparous and multiparous cows were compared to different viable isolates of staphylococci. Twelve healthy cows (six primiparous and six multiparous) were used in the middle third of lactation and 50 viable isolates of staphylococci (38 SNA and 12 Staphylococcus aureus) from different ecological niches. Neutrophil viability (P = 0.55), ROS production (P = 0.12) and phagocyte functional activity (P = 0.33) were similar among the primiparous and multiparous groups tested. However, differences (P <0.05) between the different groups of species and strains of staphylococci were observed for the stimulation of intracellular ROS production by neutrophils and phagocytosis. S. chromogenes of different origins, ceiling apex (P =0.01), transient intramammary infection (P <0.01) and persistent intramammary infection (P <0 .01) further stimulated the production of ROS by neutrophils than species. All isolates were phagocytosed by neutrophils, but S. chromogenes resisted more efficiently than the other SNA species, especially S. chromogenes isolated from the apex of the ceiling (P <0.01). S. haemolyticus isolated from apex to ceiling (P =0.02) and transient (P <0.01) intramammary infection, as well as S. fleurettii (P <0.01), were substantially phagocytosed in the same manner as S. aureus isolated from nasal swab (P = 0.03). More evident than possible variations between mammary responses of primiparous and multiparous is the variation between ANS. The more adapted to the breast, the greater resistance to phagocytosis.
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