Submitted by Celso Magalhaes (celsomagalhaes@ufrrj.br) on 2017-07-17T14:02:13Z No. of bitstreams: 1 2017 - Gabriela Nunes Mattos.pdf: 1178206 bytes, checksum: 6807a11fca850794a1c7f5b18bbed4c4 (MD5) / Made available in DSpace on 2017-07-17T14:02:13Z (GMT). No. of bitstreams: 1 2017 - Gabriela Nunes Mattos.pdf: 1178206 bytes, checksum: 6807a11fca850794a1c7f5b18bbed4c4 (MD5) Previous issue date: 2017-02-13 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / During vinification process a large volume of waste is generated, being the grape pomace, the main by-product. Due to its composition, rich in phenolic compounds and other nutrients, grape pomace can be considered as a low cost source of inputs, for example, in obtaining additives for the reduction of microbial contamination and lipid oxidation. The objective of this work was to evaluate the effect of using an extract of grape pomace from the vinification of Alicante bouschet grapes on the microbiological quality and the oxidative stability of tilapia sausages stored under refrigeration for 60 days. The powder of the extract of the grape pomace was obtained by hydroethanolic extraction and subsequent spray drying. The liquid and powder extracts were analyzed for the content of phenolic compounds, total anthocyanins and monomeric and antioxidant capacity by ABTS and ORAC methods. The sausage was produced using as raw material fillet and mechanically separated meat (CMS) of tilapia. Five sausage formulations were tested: Control (without antioxidant), Positive control (addition of synthetic antioxidant), T1% (addition of 1% of powdered extract), T0.5% (addition of 0.5% powdered extract) And T0.5% SC (addition of 0.5% powdered extract and curing salt). Tilapia sausages were analyzed for their centesimal composition, amino acid and fatty acids concentrations and lipid oxidation. The microbiological quality of the sausages was evaluated for 60 days. The bagasse extracts showed a high concentration of anthocyanins, phenolic compounds and antioxidant capacity, and after microencapsulation, the powders also showed adequate anthocyanin retention. Sausages formulated with powdered grape pomace extract presented the moisture content as recommended by Brazilian legislation, high ash and protein content, low lipid concentration, presence of essential amino acids, and it was rich in monounsaturated fatty acids. Sausages can be considered a nutritional food, as their ratio of saturated / polyunsaturated fatty acids was high. It was not verified the occurrence of lipid oxidation in all the samples along the storage, which was justified by the low concentration of the polyunsaturated fatty acids and lipid content. The grape pomace extract, the BHT and the curing salt promoted the maintenance of the microbiological quality of the sausages throughout the useful life. The acceptance test was carried out with 77 tasters and showed that the control, positive control, T1% and T0.5% SC sausages were accepted, unlike the T0.5% formulation. Considering the results obtained, the grape pomace extract showed to be a natural antioxidant potential to be used by the food industry as an additive. / No processo de vinifica??o ? gerado um grande volume de res?duo, sendo o baga?o de uva, o principal coproduto. Devido ? sua composi??o rica em compostos fen?licos e outros nutrientes, o baga?o de uva pode ser transformado em uma fonte de insumos de baixo custo, por exemplo, na obten??o de aditivos para a redu??o da contamina??o microbiana e oxida??o lip?dica. O objetivo do trabalho foi avaliar o efeito da aplica??o do extrato de baga?o de uva Alicante bouschet em p?, oriundo de vinifica??o de uvas tintas, na qualidade microbiol?gica e na estabilidade oxidativa de salsichas de til?pia armazenadas sob refrigera??o durante 60 dias. O extrato do baga?o de uva em p? foi obtido por extra??o hidroetan?lica e subsequente secagem por spray drying. Os extratos do baga?o de uva l?quido e em p? foram analisados quanto ao teor de compostos fen?licos, antocianinas totais e monom?ricas e capacidade antioxidante por ABTS e ORAC. A salsicha foi produzida usando como mat?ria-prima o fil? e a carne mecanicamente separada (CMS) de til?pia. Foram testadas cinco formula??es de salsicha: Controle (sem antioxidante), Controle positivo (adi??o de antioxidante sint?tico), T1% (adi??o de 1% de extrato em p?), T0,5% (adi??o de 0,5% de extrato em p?) e T0,5%SC (adi??o de 0,5% de extrato em p? e sal de cura). As salsichas de til?pia foram analisadas quanto ? sua composi??o centesimal, conte?do de amino?cidos, ?cidos graxos e oxida??o lip?dica. A qualidade microbiol?gica das salsichas foi avaliada por 60 dias. Os extratos do baga?o apresentaram elevada concentra??o de antocianinas, compostos fen?licos e capacidade antioxidante, e ap?s a microencapsula??o, os p?s tamb?m mostraram reten??o de antocianinas adequada. As salsichas formuladas com extrato de baga?o de uva em p? apresentaram teor de umidade conforme preconizado pela legisla??o brasileira, alta concentra??o de cinzas, baixo conte?do lip?dico e alto teor proteico, presen?a de amino?cidos essenciais, al?m de serem ricas em ?cidos graxos monoinsaturados. As salsichas podem ser consideradas de boa qualidade nutricional, uma vez que sua rela??o ?cidos graxos polinsaturados: saturados foi elevada, al?m do baixo teor de lip?dios encontrado. N?o foi verificada a ocorr?ncia de oxida??o lip?dica em todas as amostras ao longo do armazenamento, o que foi justificado pela baixa concentra??o dos ?cidos graxos polinsaturados e teor lip?dico. O extrato de baga?o de uva, o BHT e o sal de cura promoveram a manuten??o da qualidade microbiol?gica das salsichas ao longo da vida ?til. O teste de aceita??o, realizado com 77 provadores, mostrou que as salsichas controle, controle positivo, T1% e T0,5%SC foram aceitas, ao contr?rio da formula??o T0,5%. Considerando os resultados obtidos, o extrato de baga?o de uva mostrou-se um potencial antioxidante natural a ser utilizado pela ind?stria aliment?cia como aditivo.