English-style crumpets (pH 6--8, aw 0.97--0.99) are a popular baked product enjoyed by consumers worldwide. However, over the past few years, outbreaks of food poisoning have been caused by the growth of Bacillus cereus in crumpets. This spore forming microorganism, which originates in flour, can easily survive the baking process and grow to >106 CFU/g within 3--5 days at ambient storage temperature. Therefore, control of this pathogen is essential to ensure the safety and marketability of English-style crumpets. / Initial studies were done to determine the effect of water activity ( aw), pH, modified atmosphere packaging (MAP), UV-light, bacteriocins, organic acids and esters, alone and in conjunction with each other, on the growth of B. cereus in model broth/agar systems. / B. cereus is a difficult microorganism to control in food using conventional preservation methods. Further studies are now under way to investigate novel methods to control the growth of this pathogen, particularly in high pH crumpets. (Abstract shortened by UMI.)

Bacillus cereus has been responsible for several food poisoning outbreaks involving high moisture-high pH (aw ~ 0.98, pH ~ 8.8) English-style crumpets. Two novel methods, involving sorbohydroxamic acid (SHA) and mastic essential oil (MO), were evaluated for their potential to inhibit the growth of this pathogen and other selected spoilage and foodborne pathogens in high moisture, high pH English-style crumpets. / While sorbic acid only controlled the growth of B. cereus at pH 5 and 5.5, SHA proved effective at all pHs and concentrations under investigation. MO also failed to inhibit the growth of B. cereus when added directly to agar plates. / Products were unacceptable when counts increased from 103 CFUIg to 106 CFU/g or sensory scores reached <3 on a scale of 5. Only SHA (0.3% w/w) proved effective in high pH crumpets. (Abstract shortened by UMI.)

Thesis (Ph. D.)--Rutgers University, 2010. / "Graduate Program in Food Science." Includes bibliographical references (p. 122-127).

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The purpose was to find the adjustments needed to make acceptable pies, cookies and muffins baked in the electronic oven. The products were evaluated by a taste panel and by objective tests. The results of both tests supported each other. The best bottom pie crust had an extra tablespoon of liquid added to the pastry and was precooked before the filling was added. It was both flaky and tender. No leavening and one-fourth more liquid produced the most acceptable cookies. They were more like standard cookie in height, texture, moisture and tenderness. It was found that one-half the amount of egg and two tablespoons more liquid made the best muffins. No treatment of the muffins as used in this study resembled the standard, but were half way between a standard muffin and cake in their texture, flavor and appearance.

Freshness is a holistic attribute of a food product, with a meaning that most often includes how recently produced or harvested a food currently is and to what extent it has been preserved. Without knowledge of production date, consumers will judge perceived freshness by the sensory properties of foods. However, these properties are not easily identified and described, as they vary considerably between different product types. The objective of this thesis, was to determine the sensory properties that consumers most associate with baked product freshness, and to determine the volatile composition responsible for "fresh-baked" flavour. In addition, relationships were determined and modelled between volatile composition, sensory properties and perceived freshness, in order to predict perceived freshness without the need for further consumer testing. Two consumer studies were carried, where perceived freshness of 10 breads, 5 cakes and 5 biscuits was measured based on appearance, flavour and texture, and by product odour only. Individual differences in perceived freshness were represented on two-dimensional Freshness Maps generated using Principal Component Analysis (PCA). Consumers used similar terms to describe product freshness, but different terms were associated with specific product types. An understanding of the sensory characteristics associated with perceived freshness was determined by examining relationships between perceived freshness and descriptive analysis (DA). Sensory characteristics perceived to convey freshness in one product type did not necessarily convey freshness in another. Further relationships between perceived freshness and sensory characteristics were determined for 20 bread types. Descriptive analysis was carried out with all breads, whereas perceived freshness was measured for 10 of these. Three consumer clusters were identified that were homogenous in their freshness perceptions, indicating that perceived bread freshness varied among consumers. Perceived freshness of breads not evaluated by consumers, but assessed by descriptive sensory analysis, were predicted for each consumer cluster using Partial Least Squares Regression (PLSR). Most fresh breads were described as having "porous" appearance, "floury", "toasted" and "malty" odour, "sweet", "buttery", and "oily" flavour, and "sweet" aftertaste. Least fresh breads were described as "musty" odour, "sour" flavour and "sour" aftertaste. In addition, Proton Transfer Reaction Mass Spectrometry (PTR-MS) measured the volatile composition of all 20 bread types. Thirty-three mass ions significantly discriminated between bread types. Relationships were determined between odour and flavour sensory attributes, and mass spectral data using 15 of the 20 breads. PLSR models predicted the sensory properties and perceived freshness of all breads including five types not included in the construction of models. Bread perceived to be most fresh shared a similar combination of positively correlated mass ions, m/z 87, 97 and 117, also represented by "dairy" odour and "buttery" flavour, whilst the masses m/z 63, 69 and 91 were negatively associated with bread freshness, represented by the sensory attributes "grain", and "musty" odour, and "sour" flavour. The influence of sweetener, fat type and time from baking on perceived freshness was determined for cakes. Four sweeteners (sucrose, glucose, xylitol, isomaltose), three fat types (butter, margarine, shortening) and two times from baking (days zero and 15) were investigated. Descriptive analysis was carried out using a trained panel, and volatile composition of measured using PTR-MS. Twelve cake samples with the largest sensory variation were further selected for consumer testing for perceived freshness. Results demonstrated that sweetener type, fat type, time from baking and their interaction significantly influenced the sensory properties, volatile composition, and perceived freshness of cakes. There was no evidence of perceived freshness segmentation among consumers. Perceived freshness of the twelve cakes not evaluated by consumers was predicted using sensory properties, volatile composition, and their combination. The freshest cakes were evaluated on the day of baking, and contained a combination of margarine and sucrose, and butter and sucrose. The least fresh cakes were evaluated after 15 days of storage and contained a combination of shortening and isomaltose, and butter and isomaltose. The most fresh samples were positively correlated with mass ions m/z 124, 74, 97 and 93, and sensory characteristics of "buttery" odour, and "buttery", "eggy" flavour. The least fresh samples were negatively correlated with mass ions m/z 110 and 95, and sensory characteristics of "rancid", "dusty" and "fatty" odour. Best predictions were obtained for cakes evaluated on day zero that contained a combination of shortening and sucrose. This research established an objective knowledge of consumers' perceived freshness of baked product types in terms of sensory properties and volatile composition. This approach enabled the understanding of ingredients and time from baking influences, as well their interaction on baked product freshness. The predictive models developed that examined relationships between PTR-MS spectra, sensory characteristics and consumer perceived freshness can be applied to predict freshness of baked products not assessed by consumers.

In this research, initial studies were done to determine the effect of various methods of presentation involving chemical preservatives, water activity ($ rm a sb{w}$), and modified atmosphere packaging (MAP) on mold growth in an agar model system. Results showed that preservatives could completely inhibit mold growth for 2-40d depending on concentration and pH used. Gas packaging (60% or 80% CO$ sb2$), oxygen absorbents, alone or in combination with potassium sorbate, could also inhibit mold growth for $>$40d at ambient storage temperature using a Response Surface Methodology (RSM) approach. / The effects of various methods of applying potassium sorbate into pizza crusts via direct incorporation into the batter, surface spraying, and impregnation of packaging material with potassium sorbate to control mold spoilage of pizza crusts were also investigated. Results showed that the antimicrobial effect of potassium sorbate was negligible when the packaging material was impregnated with the inhibitor but more pronounced when it was incorporated directly into the dough or sprayed onto the product's surface. The inhibitory effect of potassium sorbate increased as both the pH and the inoculum level decreased. / Shelf life studies using low concentrations of potassium sorbate (1000 and 2000 p.p.m.) and MAP, alone and in combination with each other, showed that potassium sorbate, gas packaging or oxygen absorbents (Ageless FX) could extend the shelf life of pizza crusts and decrease the growth rate of molds, bacteria and yeast. Furthermore, when pizza crusts were packaged in 60% CO$ sb2$ or with an oxygen absorbent, in combination with potassium sorbate (1000-2000 p.p.m.), a shelf life of 42d was possible without compromising the sensory shelf life of the product. (Abstract shortened by UMI.)

Thesis (Ph. D.)--Electrical and Computer Engineering, Georgia Institute of Technology, 2005. / Dr. Jennifer E. Michaels, Committee Chair ; Dr. Bonnie Heck Ferri, Committee Member ; Dr. George J. Vachtsevanos, Committee Member ; Dr. Magnus Egerstedt, Committee Member ; Dr. Farrokh, Ayazi, Committee Member ; Dr. Sheldon M. Jeter, Committee Member. Vita. Includes bibliographical references.