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DNA SELF-ASSEMBLY DRIVEN BY BASE STACKINGLongfei Liu (6581096) 10 June 2019 (has links)
<p>DNA nanotechnology has provided programming construction of
various nanostructures at nanometer-level precision over the last three
decades. DNA self-assembly is usually implemented by annealing process in bulk
solution. In recent several years, a new method thrives by fabricating
two-dimensional (2D) nanostructures on solid surfaces. My researches mainly
focus on this field, surface-assisted DNA assembly driven by base stacking. I
have developed methods to fabricate DNA 2D networks via isothermal assembly on
mica surfaces. I have further explored the applications to realize quasicrystal
fabrication and nanoparticles (NPs) patterning.</p><p><br></p>
<p>In this dissertation, I have developed a strategy to assemble DNA
structures with 1 or 2 pair(s) of blunt ends. Such weak interactions cannot
hold DNA motifs together in solution. However, with DNA-surface attractions,
DNA motifs can assemble into large nanostructures on solid surface. Further
studies reveal that the DNA-surface attractions can be controlled by the
variety and concentration of cation in the bulk solution. Moreover, DNA
nanostructures can be fabricated at very low motif concentrations, at which
traditional solution assembly cannot render large nanostructures. Finally,
assembly time course is also studied to reveal a superfast process for
surface-assisted method compared with solution assembly.</p><p><br></p>
<p>Based on this approach, I have extended my research scope from 1D to 2D
structures assembled from various DNA motifs. In my studies, I have
successfully realized conformational change regulated by DNA-surface
interaction and steric effect. By introduction of DNA duplex “bridges” and
unpaired nucleotide (nt) spacers, we can control the flexibility/rigidity of
DNA nanomotifs, which helps to fabricate more delicate dodecagonal quasicrystals.
The key point is to design the length of spacers. For 6-point-star motif, a
rigid structure is required so that only 1-nt spacers are added. On the other
hand, 3-nt spacers are incorporated to enable an inter-branch angle change from
60° to 90° for a more flexible 5-point-star motif. By tuning the ratio of 5 and
6 -point-star motifs in solution, we can obtain 2D networks from snub square
tiling, dodecagonal tiling, a
mixture of dodecagonal tiling and triangular tiling, and triangular tiling.</p><p><br></p>
Finally,
I have explored the
applications of my assembly method for patterning NPs. Tetragonal and hexagonal
DNA 2D networks have been fabricated on mica surfaces and served as templates.
Then modify the surfaces with positively-charged “glues”, <i>e.g.</i> poly-L-lysine (PLL) or Ni<sup>2+</sup>. After that, various
NPs have been patterned into designated lattices, including individual DNA
nanomotifs, gold NPs (AuNPs), proteins, and silica complexes. Observed NP
lattices and fast Fourier Transform (FFT) patterns have demonstrated the DNA
networks’ patterning effect on NPs.
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Reaction coordinates for RNA conformational changesMohan, Srividya 06 April 2009 (has links)
This work investigates pathways of conformational transitions in ubiquitous RNA structural motifs. In our lab, we have developed multi-scale structural datamining techniques for identification of three-dimensional structural patterns in high-resolution crystal structures of globular RNA. I have applied these techniques to identify variations in the conformations of RNA double-helices and tetraloops. The datamined structural information is used to propose reaction coordinates for conformational transitions involved in double-strand helix propagation and tetraloop folding in RNA. I have also presented an algorithm to identify stacked RNA bases. In this work, experimentally derived thermodynamic evaluation of the conformations has been used to as an additional parameter to add detail to RNA structural transitions.
RNA conformational transitions help control processes in small systems such as riboswitches and in large systems such as ribosomes. Adopting functional conformations by globular RNA during a folding process also involves structural transitions. RNA double-helices and tetraloops are common, ubiquitous structural motifs in globular RNA that independently fold in to a thermodynamically stable conformation. Folding models for these motifs are proposed in this work with probable intermediates ordered along the reaction coordinates.
We hypothesize that frequently observed structural states in crystals structures are analogous in conformation to stable thermodynamic â on-pathwayâ folded states. Conversely, we hypothesize that conformations that are rarely observed are improbable folding intermediates, i.e., these conformational states are â off-pathwayâ states. In general on-pathway states are assumed to be thermodynamically more stable than off-pathway states, with the exception of kinetic traps.
Structural datamining shows that double helices in RNA may propagate by the â stack-ratchetâ mechanism proposed here instead of the commonly accepted zipper mechanism. Mechanistic models for RNA tetraloop folding have been proposed and validated with experimentally derived thermodynamic data. The extent of stacking between bases in RNA is variable, indicating that stacking may not be a two-state phenomenon. A novel algorithm to define and identify stacked bases at atomic resolution has also been presented in this work.
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Femtosecond Transient Absorption Study of the Excited-State Dynamics of Single-Stranded Adenine-Containing Multinucleotides and Steady-State Absorption Spectroscopy of Mononucleotides in Cryogenic Water/Ethylene Glycol MatricesSu, Charlene 02 November 2010 (has links)
No description available.
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The use of UV resonance Raman spectroscopy in the analysis of ionizing radiation-induced damage in DNAShaw, Conor Patrick 14 December 2007 (has links)
Raman spectroscopy is a form of vibrational spectroscopy that is capable of probing biological samples at a molecular level. In this work it was used in the analysis of ionizing radiation-induced damage in DNA. Spectra of both simple, short-stranded DNA oligomers (SS-DNA) and the more complicated calf-thymus DNA (CT-DNA) were acquired before and after irradiation to a variety of doses from 0 to ~2000 Gy. In a technique known as ultraviolet resonance Raman spectroscopy (UVRRS), three UV wavelengths of 248, 257 and 264 nm were utilized in order to selectively enhance contributions from different molecular groups within the samples. Assignment of the spectral peaks was aided by the literature, as well as through analysis of UVRR spectra of short strands of the individual DNA bases obtained at each of the three incident UV wavelengths. Difference spectra between the irradiated and unirradiated samples were calculated and the samples exposed to ~2000 Gy showed significant radiation-induced features. Intensity increases of spectral peaks, observed primarily in the CT-DNA, indicated unstacking of the DNA bases and disruption of Watson-Crick hydrogen bonds, while intensity decreases of spectral peaks, observed only in the SS-DNA, indicated both base damage and the loss of structural integrity of the DNA molecule. The high molecular specificity of UVRRS allowed for precise identification of the specific bonds affected by the radiation, and the use of the varying incident wavelengths allowed for the observation of damage to moieties that would otherwise have been excluded. The use of UVRRS shows promise in the study of radiation-induced damage to DNA and would be well suited for extension to the study of more complicated biological systems.
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The use of UV resonance Raman spectroscopy in the analysis of ionizing radiation-induced damage in DNAShaw, Conor Patrick 14 December 2007 (has links)
Raman spectroscopy is a form of vibrational spectroscopy that is capable of probing biological samples at a molecular level. In this work it was used in the analysis of ionizing radiation-induced damage in DNA. Spectra of both simple, short-stranded DNA oligomers (SS-DNA) and the more complicated calf-thymus DNA (CT-DNA) were acquired before and after irradiation to a variety of doses from 0 to ~2000 Gy. In a technique known as ultraviolet resonance Raman spectroscopy (UVRRS), three UV wavelengths of 248, 257 and 264 nm were utilized in order to selectively enhance contributions from different molecular groups within the samples. Assignment of the spectral peaks was aided by the literature, as well as through analysis of UVRR spectra of short strands of the individual DNA bases obtained at each of the three incident UV wavelengths. Difference spectra between the irradiated and unirradiated samples were calculated and the samples exposed to ~2000 Gy showed significant radiation-induced features. Intensity increases of spectral peaks, observed primarily in the CT-DNA, indicated unstacking of the DNA bases and disruption of Watson-Crick hydrogen bonds, while intensity decreases of spectral peaks, observed only in the SS-DNA, indicated both base damage and the loss of structural integrity of the DNA molecule. The high molecular specificity of UVRRS allowed for precise identification of the specific bonds affected by the radiation, and the use of the varying incident wavelengths allowed for the observation of damage to moieties that would otherwise have been excluded. The use of UVRRS shows promise in the study of radiation-induced damage to DNA and would be well suited for extension to the study of more complicated biological systems.
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Mutagenicity of 5-bromouracil : quantum chemical studyHolroyd, Leo January 2015 (has links)
This thesis describes a computational investigation of the mutagenicity of 5-bromouracil (BrU). In Chapter 1, three models of spontaneous and BrU-induced base mispairing (rare tautomer, wobble pair, and ion) are reviewed. Chapter 2 presents the computational techniques used: electronic structure methods (Hartree–Fock-based and density functional theory) and molecular dynamics. Chapter 3 presents optimisations of the keto and enol tautomers of BrU and uracil (U) in water clusters. The enol tautomer of BrU is found to be more stable than that of U. Chapter 4 is a molecular dynamics study of the keto-enol tautomerism of BrU and U in a periodic water box. The pKₐ of BrU at N3 is found to be lower than that of U. Chapter 5 is a study of stacked base dimers containing BrU, U, or thymine (T) stacking with natural bases. Some structures were taken from the Protein Data Bank, while others were generated using an in-house methodology. BrU is found to stack more strongly than T in vacuo, but solvation and thermal effects nullify this difference. Chapter 6 discusses the significance of the results in Chapters 3–5 in terms of BrU-induced mutagenesis. Appendices A and B–D provide supplementary material to Chapters 2 and 5, respectively. Appendix E is an investigation of the “base flipping” pathway of 2-aminopurine (2AP). Both 2AP/N and A/N dinucleosides (N = thymine or guanine) are found to adopt a wide range of energy-minimum conformations – not only stacked and “flipped”, but also intermediate – and the stacked are not the most favourable by free energy. Appendix F is a list of publications and papers in preparation. One publication concerns BrU stacking. The other is a conformational study of the dipeptide tyrosine-glycine: the theoretical results are shown to be consistent with experiment (R2PI spectra) if thermal effects are taken into account.
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Promotion and Inhibition of Molecular Recognition at Interfaces in Aqueous SolutionMa, Mingming 17 December 2010 (has links)
No description available.
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