Hollow fills are composed of excess spoil and debris produced from surface coal mining that is not returned to the original mined site. Hollow fills are often constructed in the head of hollows nearby or adjacent to the mined land area, which may be the origins of headwater streams or drain into low order systems. Eleven hollow fills were utilized in evaluating the influence fill drainages had on low order streams in Virginia and West Virginia. The study was conducted in six watersheds including; Five Mile Creek in Mingo County, West Virginia, Trace Fork in Mingo County, West Virginia, Lavender Fork in Boone County, West Virginia, Middle Creek in Tazewell County, Virginia, South Fork of the Pound River in Wise County, Virginia, and Powell River in Wise County, Virginia. Bioassessment procedures used in the evaluation of hollow fill drainages included water/sediment chemistry, acute water column toxicity testing using <i>Ceriodaphnia dubia</i>, chronic sediment toxicity testing using <i>Daphnia magna</i>, benthic macroinvertebrate surveys, and <i>in situ</i> Asian clam (<i>Corbicula fluminea</i>) toxicity testing. Common significant differences in water quality between reference and fill influenced sites, among all watersheds, were elevated conductivity and water column metal concentrations, particularly aluminum and copper. Water column and sediment toxicity testing reported limited significant mortality or reproductive impairment associated with hollow fill drainages. The West Virginia watersheds used in the study consisted of headwater streams originating directly from the settling ponds, placed at the base of the hollow fills, receiving drainages from the fills. Benthic macroinvertebrate analysis reported no significant alteration in total taxa or EPT richness downstream of the ponds. Yet, collector filterer populations, including benthic macroinvertebrates and <i>in situ</i> Asian clams, were enhanced directly downstream of the ponds due to organic enrichment originating from the ponds. A decrease in collector filterer populations and lowered clam growth suggested the organic enrichment dissipated downstream from the ponds. Chlorophyll <i>a</i> analysis of the phytoplankton community was not significantly related to the enhance collector filterer populations in the streams, however the high concentrations in the settling ponds suggest abundant algal communities. The hollow fills evaluated in Virginia drained into receiving systems, whose headwater origins were not directly related to hollow fill drainages. Low taxa richness was associated with the hollow fill and settling pond drainages, however receiving system sites were minimally influenced. Yet, as reported in the West Virginia watersheds, the settling ponds input organic enrichment that enhanced collector filterer populations, including benthic macroinvertebrates and <i>in situ</i> test clams. An analysis of the hollow fills' age, or maturity, reported no significant difference between young and old fills. In general, a common feature of among the various aged fill drainages was elevated conductivity, compared to reference sites of the watersheds. / Master of Science

The aim of this thesis has been to: 1) assess the state of pollution in the two study areas and relate them to recent changes found in the Kattegat - Belt Sea area; 2) examine the observed spatial and temporal variability in species composition, abundance and biomass in Arhus Bay and the Formes area and relate the variability to antropogenic and natural causes; 3) discuss and assess methods, particularly for the estimation of secondary production and the use of multivariate analyses as methods for examining changes in macrozoobenthic communities. Macrozoobenthos were sampled at 15 stations in Arhus Bay, Denmark from 1985 to 1991 while data from Formes (reference area) included 55 sampling stations from 1986 to 1990. Monthly sampling took place at one station in Arhus Bay in 1990 and 1991. The two study areas are both situated on the eastcoast of Jutland in the Kattegat - Belt Sea area at 13-17 m depth and both receive waste water from long sea outfalls. Although both areas are Subjected to salinity stratification for most of the year, the exposed position of the Formes area on the open Kattegat coast prevents it from suffering from severe oxygen deficiencies, unlike the Arhus Bay which is a sheltered, semi-enclosed sedimentation area where oxygen concentrations in the bottom water can be very low. At Formes the sediment is sandy while it is silty in Arhus Bay. The spatial and temporal variability in the benthos in Arhus Bay could to a great extent be explained by the variation in 7 important species: Abra alba, Corbula gibba, Mysella bidentata, Nepthys hombergii, N. ciliata, Ophiura albida and Echinocardium cordatum. The fluctuations in the number and biomass of A. alba had a pronounced effect on the total abundance and biomass in Arhus Bay. The severe winter of 1986/87 with low temperatures and oxygen depletion under the ice cover practically eliminated A. alba from the bay. A. alba quickly recolonized the area and was found in high numbers in 1988. Studies of growth of A. alba in 1990 and 1991 showed that by the end of 1990 the population had reached an average length of 10 mm while the average shell length was only 5 mm by the end of 1991. The difference between the two years could be attributed to the difference in sedimentation of phytoplankton from the water column. As in other parts of the Kattegat - Belt Sea area, Arhus Bay has experienced low oxygen concentrations in the bottom water in late summer early autumn throughout the 1980s. Only the oxygen depletion under the ice cover in early spring 1987 and the local oxygen deficiencies south of the outlet in 1989 and 1990 actually killed parts of the benthic fauna. Apart from 1981, the oxygen deficiencies have thus been less severe in Arhus Bay than in other parts of the southern Kattegat in the 1980s. The number of species, abundance and biomass decreased at Fornres from 1980 to 1985 while the discharge of BOD was fairly constant during the same period. From 1986 there was a slight decrease in the discharge of BOD but a considerable increase in the number of species, abundance and biomass. At least for the second half of the 1980s there was no straightforward relation between the organic enrichment from the outlet and species composition, abundance and biomass and suggests that other factors are also important influencing the fluctuations in the benthic fauna. Estimates of total secondary community production were found to be very dependent on the method used. The method described by Brey (1990) was found acceptable for estimating secondary production in Arhus Bay but care should be exercised when comparisons are made with other areas where different methods have been used to estimate production. Secondary production was estimated more accurately for some of the abundant species in Arhus Bay on the basis of monthly samplings by the method described by Crisp (1984). Among the multivariate analyses the Detrended Correspondence Analysis (DCA) and nonmetric Multidimensional Scaling (MDS) proved to be the most successful with the Arhus Bay and Fornes data sets. Two Way INdicator SPecies ANalysis (TWINSPAN) did not work well with the Fornes data because it imposed discontinuities on data sets with continous variation in distribution of species among samples. As community types existed to a certain degree in Arhus Bay TWINSPAN worked well with these data. DCA and MDS were found to be useful techniques for analysing large data sets because they can summarize the data matrices to a manageable form and find possible patterns in the data sets. The results of the analyses can then be used as starting point for more detailed investigations of single species/samples or groups of species/samples. By using different transformations of the raw data the role of dominant or rare species can be assessed. A major 'problem in the assessment of multivariate techniques is the lack of external standards to compare with. The results of multivariate analyses must therefore be assessed critically on the basis of a careful examination of the species list combined with the knowledge and experience of the investigator. The methods used all had their advantages and limitations but each of the different methods added some important information to the picture of the benthic community in Arhus Bay and Fornes. It was thus an considerable advantage to use several different methods to analyse the spatial and temporal variability in the benthic fauna in relation to antropogenic and natural causes.

The Arctic region has experienced the largest degree of anthropogenic warming, causing rapid, yet variable sea-ice loss. The effects of this warming on the Canadian Beaufort Shelf have led to a longer ice-free season which has assisted the expansion of northern development, mainly in the oil and gas sector. Both these direct and indirect effects of climate change will likely impact the marine ecosystem of this region, in which benthic fauna play a key ecological role. The aim of this thesis was to expand the current baseline knowledge of benthic fauna in the interest of developing the capacity to identify, predict and manage benthic change. The distribution of benthic macro- and megafauna was characterized utilizing community data from two recent benthic surveys on the Canadian Beaufort shelf and slope. Fauna were collected from 63 stations using box core and trawl sampling gear over the summers of 2009 through 2012 between depths of 30 and 1,000 m. Spatial patterns of abundance, biomass and α and β diversity metrics were examined. Megafaunal abundance and α diversity were elevated on the shelf compared to the slope while the macrofauna did not vary significantly with depth. Multivariate analyses illustrated that both macro- and megafaunal community composition varied more across the depth gradient than from east to west along the shelf. However the change across the depth gradient was greater for the megafauna than for the macrofauna. I proposed that megafaunal slope taxa were differentiated from shelf taxa, as faunal replacement not nestedness appeared to be the main driver of megafaunal β diversity across the depth gradient. The lack of correlation between macro- and megafauna in abundance, biomass and α and β diversity suggests that these faunal components vary at different spatial scales. These results demonstrate how separately sampling the different benthic components can yield different spatial patterns, with implications for future benthic monitoring in the region. This work contributes to the current regional baselines by providing the first comprehensive description of megafaunal distribution on the Canadian Beaufort shelf and by extending our knowledge of benthic distribution patterns deeper on the slope. / Graduate / 0329

Thesis (M. Phil.)--University of Hong Kong, 1999. / Includes bibliographical references (leaves 104-118).

Thesis (M.A.)--West Virginia University, 2000. / Title from document title page. Document formatted into pages; contains ix, 121 p. : ill. (some col.), maps (some col.). Includes abstract. Includes bibliographical references (p. 117-121).

Stream living salmonids are generally regarded as drift feeders that rely upon their vision when foraging. It has been shown that salmonids become nocturnal at low water temperatures, but have a low foraging efficiency as light intensity is low, due to their dependence upon vision. Shifting from drift feeding to benthic feeding, has been suggested, and analyses of gut contents during winter have shown that the diet of salmonids mainly consists of benthic invertebrates. Most experimental studies of salmonid foraging have only offered the fish drifting prey or only given the fish access to benthic prey in total darkness. Such conditions rarely occur in nature and the importance of benthic foraging to salmonids may therefore have been underestimated. In this study I conducted a stream laboratory experiment to test if low light intensity caused juvenile Atlantic salmon (Salmo salar) and brown trout (Salmo trutta) (age 0+) to forage more on benthic than drifting prey. The salmon foraged on both drifting and benthic prey during high light but consumed only benthic prey during low light (by one of six fish). Trout foraged on both drifting and benthic prey during both high and low light, but foraging efficiency was lower during low than high light and foraging efficiency was lower for benthic prey than for drifting prey. These results indicate that both species forage more opportunistically than previously thought. / Strömlevande laxfiskar anses generellt vara driftätare som förlitar sig på synen när de födosöker. Det har visats att laxfiskar blir nattaktiva vid låga vattentemperaturer, men har en låg födosökseffektivitet då ljusintensiteten är låg, på grund av sitt beroende av synen. Skifte från driftätande till att äta bentiska byten, har föreslagits, och analyser av maginnehåll under vintern har visat att laxfiskars diet huvudsakligen består av bentiska evertebrater. De flesta experimentella studier av laxfiskars födosök har endast erbjudit fisken driftande byten eller endast gett fisken tillgång till bentiska byten vid totalt mörker. Sådana förhållanden förekommer sällan i naturen och betydelsen av bentiskt födosök för laxfiskar kan därför ha underskattats. I den här studien utförde jag ett experiment i en laboratorieström för att testa om låg ljusintensitet fick juvenil Atlantlax (Salmo salar) och öring (Salmo trutta) (ålder 0+) att födosöka mer på bentiska än driftande byten. Laxen födosökte på både driftande och bentiska byten vid högt ljus men bara bentiska byten åts vid lågt ljus (av en av sex fiskar). Öringen födosökte på både driftande och bentiska byten vid både hög och låg ljusintensitet, men födosökseffektiviteten var lägre vid låg än hög ljusintensitet och födosökseffektiviteten var lägre för bentiska än förbiflytande byten. Dessa resultat indikerar att båda arterna födosöker mer opportunistiskt än vad man tidigare ansett.

Coastal and shallow lakes are often subjected to eutrophication due to nutrients from catchment farming activities. Lake Ellesmere (Te Waihora) is a hyper-eutrophic lake which has gained recent attention because of concerns over its ecological health and fishery status. This study investigated the benthic ecology of the lake by extensive spatial and temporal sampling. Eight littoral sites were sampled on a single occasion, and 20 benthic sites were sampled once per season for one year. Water chemistry conditions, substrate and invertebrate communities varied significantly around the lake. Salinity, pH, DO and seston were primarily affected by freshwater inputs from inflow streams and salt water intrusion due to the lake opening to the sea. On these occasions, salinity reached 32 ‰ at the lake outlet. The lake invertebrate community was depauperate, comprising of only two species of invertebrate predators restricted to the littoral zone and eight benthic invertebrate taxa, dominated by oligochaetes, amphipods and chironomids. Benthic invertebrate abundances also reflect the dominant local substrate, where oligochaetes and chironomids preferred areas of silt substrate, whereas Potamopyrgus preferred harder substrate. Stable isotope and gut analysis determined that the primary food sources within the lake were phytoplankton and algae. Macrophytes provided a minimal contribution to the food web, possibly relating to the change in status from a clear water, macrophyte dominated lake to a turbid, phytoplankton dominated condition since the Wahine Storm in 1968. Isotope analysis also showed that the lake food web was markedly different in its carbon values from food webs of its inflow streams and nearby marine source. However the lake food web did show a marine-derived carbon signature. A mesocosm experiment testing the effect of common lentic predators on the abundance of the lake chironomid Chironomus zealandicus, showed that if invertebrate predators were present in the lake they could markedly reduce the abundance of the pest prey species. This study highlights that the frequent re-suspension of bottom sediments, lake level fluctuation resulting in wetting and drying of littoral zones, and the management of the lake opening to the sea all have an effect on the benthic ecology of Te Waihora.

The term hydropeaking refers to anthropogenically induced, short-duration, high-magnitude discharge pulses that are generated in lotic systems for electricity production. The practice of hydropeaking produces the largest source of renewable energy worldwide, and its use is projected to increase through the year 2040. The primary objective of this work was to evaluate the effects of hydropeaking on benthic macroinvertebrate assemblages, which are important components of lotic ecosystems. Results of this work show that, across a wide range of impacted systems worldwide, the consistently observed patterns of elevated benthic macroinvertebrate drift in response to hydropeaking pulses are primarily related to the rate at which discharge is increased (i.e., ramping rate) and secondarily to the time between pulses. In addition, it was shown that taxa inhabiting depositional habitat patches (i.e. fine substrates and slow water velocities) were most susceptible to peaking-induced drift, and that these taxa were also those most prevalent in hydropeaking-impacted systems. Collectively, these results suggest that increased pulse ramping rate and the resulting elevated macroinvertebrate drift may be positive selective forces, which benefit populations adapted for life in hydropeaking-impacted lotic ecosystems. These results provide a greater understanding of the factors that are most important for governing the effects of hydropeaking on benthic assemblages.

As atividades agrícolas, principalmente as monoculturas, exigem grande utilização de insumos. A aplicação constante de defensivos agrícolas, como herbicidas, pesticidas e fungicidas, tem promovido crescentes impactos sobre os recursos hídricos e biota neles presentes, principalmente em organismos não alvo dessas substâncias. O presente estudo teve como principal objetivo avaliar os efeitos do fungicida Pyrimethanil na comunidade de macroinvertebrados bentônicos, por meio do monitoramento ambiental em unidades experimentais de campo (mesocosmos) e ensaios ecotoxicológicos laboratoriais com indivíduos da espécie Chironomus sancticaroli. Os mesocosmos foram construídos utilizando-se seis caixas de água com volume equivalente a 1500 litros. A contaminação dos mesocosmos com Pyrimethanil foi realizada por meio de pulverização direta na superfície da água aplicando-se uma concentração equivalente a 0,1 mg/L de Pyrimethanil nos mesocosmos contaminados. Dessa maneira, os mesocosmos foram divididos em três réplicas contaminadas e três réplicas controles. O monitoramento e coleta de amostras de água e macroinvertebrados bentônicos foram realizados com frequência mensal, durante o período de um ano. O fungicida Pyrimethanil não ocasionou alterações significativas nas variáveis monitoradas. Da mesma forma, a estrutura comunitária de macroinvertebrados bentônicos apresentou características semelhantes entre os mesocosmos contaminados e controles. Entretanto, durante o monitoramento, os mesocosmos controles apresentaram valores significativamente mais elevados na abundância de indivíduos e quantidade total de táxons quando comparados aos valores observados nos mesocosmos contaminados com Pyrimethanil. / Agricultural activities, mainly monocultures, require large use of inputs. The constant application of agrochemicals, as herbicides, pesticides and fungicides, has promoted impacts on water resources and biota at the center, especially on non-target organisms such substances. This study aimed to evaluate the effects of fungicide Pyrimethanil in benthic macroinvertebrate community through environmental monitoring experimental units of field (mesocosms) and laboratory ecotoxicological tests with individuals of Chironomus sancticaroli. The mesocosms were made using six tanks with maximum volume equivalent to 1500 liters. The contamination of the tanks with Pyrimethanil was performed by spraying of water on the surface by applying a fungicide concentration equivalent to 0,1 mg/L. Thus, the mesocosms were divided into three contaminated replicas and three controls replicas. The monitoring and sampling of water and benthic macroinvertebrates were performed on a monthly basis during the period of one year. The fungicide Pyrimethanil does not cause significant changes in monitored variables. Similarly, the community structure of benthic macroinvertebrates were similar characteristics between the contaminated and control mesocosms. However, during the monitoring, the mesocosms controls had significantly higher values in the abundance of individuals and total number of taxa compared to the values observed in the mesocosms contaminated with Pyrimethanil.

Com o intuito de conhecer o histórico da comunidade bentônica no reservatório do Guarapiranga para futuro estudo sobre a influência das diferentes formas de poluição na represa sobre o zoobentos, este trabalho foi desenvolvido a fim de coletar dados históricos da fauna de fundo e se houve variação na estrutura da comunidade ou não. Foram analisados quatro trabalhos realizados no período de inverno em quatro regiões médio-profundal do reservatório (Guarapiranga, Embu-Mirim, Embu-Guaçu e Parelheiros) ao longo dos anos, 1939, 1976, 1996 e 2010. A riqueza de dados presentada nos trabalhos refletiu a evolução do conhecimento em estudos limnológicos no Brasil e no mundo. A densidade de Oligochaeta apresentou aumento ao longo dos anos, assim como a de Chaoboridae. Por outro lado, Chironomidae que apresentava 30% em 1974 é pouco representativo em 2010. O ambiente mostrou forte e crescente enriquecimento orgânico em todos os pontos ao longo dos anos e isso colaborou para uma maior diversidade de grupos detritívoros (Tubificinae e Naidinae - Oligochaeta), por exemplo. Os níveis de oxigênio dissolvido no fundo diminuíram ao longo dos anos em todos as regiões. Chama-se a atenção para a necessidade de biomonitoramento nas 4 regiões da represa, utilizando inclusive os macroinvertebrados bentônicos e análises físicas e químicas do sedimento e a adoção de uma nova forma de gerenciamento deste manancial / With the purpose to know the history of the benthic community in Guarapiranga reservoir for future study about the influence of different sources of pollution in the reservoir over zoobenthos, this study was conducted to collect historical data of benthic fauna and variation in community structure. Four studies conducted during winter in four regions of the reservoir medium-profundal (Guarapiranga, Embu-Mirim, Embu-Guaçu and Parelheiros) over the years, 1939, 1976, 1996 and 2010 were analysed. The best richness of data presented in the works reflected the evolution of knowledge in limnological studies, in Brazil and worldwide. The density of Oligochaeta had an increase over the years as well as the Chaoboridae. On the other hand, Chironomidae presented 30% in the 70\' community and was not representative anymore in 2010. The environment showed strong organic enrichment for all regions along the years, and this has contributed to a greater diversity of detritivorous groups (Tubificinae and Naidinae - Oligochaeta), for example. The dissolved oxygen levels at the bottom declined over the years in all regions. Attention is called to the necessity of biomonitoring in 4 regions of the reservoir, using benthic macroinvertebrates and physical and chemical sediment analyses and the adoption of a new way of managing this system