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Exploring the Free Energy Landscape of RNA Stem-loop FoldingJang, Sukjin Steve January 2023 (has links)
It has long been recognized that our understanding of how RNA adapts its complex three-dimensional structure and undergoes conformational fluctuations has played a central role in our understanding of the biological functions of RNA. Our current understanding of the vast and diverse set of RNA conformational dynamics is the culmination of several decades of biophysical research applying several ensemble and single-molecule techniques.
In this journey, each of the biophysical techniques have provided a unique perspective into the dynamic processes of RNA and revealed information about distinct RNA dynamics occurring over a broad range of timescales. In recent years, a new, promising single-molecule biophysical technique called single-molecule field effect transistors (smFETs) has been developed. Because smFETs do not rely on fluorophore reporters of conformation or mechanical (un)folding forces, they provide a unique approach that enables single-molecule studies of RNA conformational dynamics observed at microsecond temporal resolution for a long period of time. The broad range of timescales opens immediate prospects for smFETs to provide a unique perspective into understanding RNA conformational dynamics that are presently inaccessible in other single-molecule approaches.
The primary focus of this thesis is to understand how RNA stem-loops undergo folding and unfolding. Stem-loops are one of the most common secondary structural motifs in RNA and act as a fundamental building block for complex RNA structures. Despite their fundamental importance, a complete unifying picture of the folding mechanism of RNA stem-loops has been difficult to achieve, primarily due to the rugged nature of their folding energy landscapes. In Chapter 2, experimental methods that were developed to enable smFET studies of RNA conformational dynamics are described. This includes the development of a high-throughput fabrication process that generates high signal to noise ratio (SNR) smFET devices and the development and validation of nucleic acid tethering strategies that enables controlled tethering of biomolecules onto smFET devices.
Utilizing these methods, Chapter 3 establishes smFET as a general single-molecule approach to characterize the folding dynamics of RNA stem-loops. Finally, Chapter 4 explores the use of smFETs to investigate the molecular mechanism in which a model RNA stem-loop undergoes folding and unfolding. Collectively, this thesis demonstrates how smFETs can be applied to uniquely capture and describe the folding energy landscapes of RNA and reveal new insights to how RNAs fold and unfold.
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Electronic spectroscopy of biological relevant species and their complexes with solvent moleculesHe, Yonggang 27 January 2005 (has links)
In this dissertation, I present electronic spectroscopy of a few biologically
relevant species and their complexes with solvent molecules in the gas phase using
a variety of techniques, including resonantly enhanced multiphoton ionization
(REMPI), laser induced fluorescence (LIF), and zero kinetic energy (ZEKE)
photoelectron spectroscopy. My work on several methylated uracils and thymines
and thymine-water complexes alludes to a new interpretation with regard to the
origin of the photostability of our genetic code. I believe that it is the water solvent
that stabilizes the photophysical and photochemical behavior of these bases under
UV irradiation. For systems that demonstrate vibrational resolution in the first
electronically excited state (S₁) and the cationic state, I performed vibrational
analysis of both states with the aid of ab initio and density functional calculations.
These observations are explained in terms of the structural changes from the
ground state to S₁ and further to the cation. To bridge results from the gas phase to
the solution phase, I also report studies of supersonically cooled water complexes
of the three isomers of aminobenzoic acid. Density functional theory calculations are carried out to identify structural minima of water complexes in the ground state.
The solvation mechanism is investigated based on vibrational analysis of the S₁
state of the neutral complex and the shift of ionization thresholds with increasing
water content. / Graduation date: 2005
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Probing allosteric coupling and dynamics with solid-state NMRSun, Zhiyu January 2022 (has links)
Solid-state NMR (ssNMR) has matured into a versatile method to provide structural information, probe protein dynamics and detect small molecule binding and -protein interaction of a variety of biomolecular assemblies including amyloid fibrils, viral particles and membrane proteins. Membrane proteins embedded in liposomes are natural targets for ssNMR as their native states are solids. Magic angle spinning (MAS) ssNMR studies using moderate spinning frequencies provide detailed structural information and probe subtle conformational change. Development of fast magic angle spinning ssNMR enables proton-detection which increases sensitivity and facilitates protein dynamics measurements. In this dissertation, we applied moderate and fast MAS ssNMR to study potassium ion channel and protein dynamics Chapter 1 will introduce concepts and theory of solid-state NMR pulse sequences and experiments. Chapter 2 will discuss the application and perspectives of solid-state NMR to membrane protein systems.
In Chapter 3, we test an allostery mechanism for inactivation using a KcsA mutant (H25R/E118A) that exhibits an open pH gate across a broad range of pH values. We present solid-state NMR measurements of this open mutant at neutral pH to probe the affinity for potassium at the selectivity filter. This result strongly supports our assertion that the open pH gate allosterically affects the potassium binding affinity of the selectivity filter. In this mutant the protonation state of a glutamate residue (E120) in the pH sensor is sensitive to potassium binding, suggesting that this mutant also has flexibility in the activation gate and is subject to transmembrane allostery.
In Chapter 4, I optimize protein expression, purification and reconstitution into native environment protocols of a bacterial potassium transporter, KtrB. In chapter 5, methods and experimental details of setting up 60 and 40 kHz fast MAS ssNMR are discussed. With fast MAS ssNMR setup, multidimensional NMR experiments with higher sensitivity could be collected on a perdeuterated sample with less sample mass required. In Chapter 6, we employ fast MAS ssNMR to measure bulk and residue site-specific 15N and carbonyl 13C relaxation of microcrystalline ubiquitin. Carbonyl R1ρ relaxation profiles provide additional information on protein backbone dynamics.
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Analysis of macromolecular structure through experiment and computationGossett, John Jared 08 April 2013 (has links)
This thesis covers a wide variety of projects within the domain of computational structural biology. Structural biology is concerned with the molecular structure of proteins and nucleic acids, and the relationship between structure and biological function. We used molecular modeling and simulation, a purely computational approach, to study DNA-linked molecular nanowires. We developed a computational tool that allows potential designs to be screened for viability, and then we used molecular dynamics (MD) simulations to test their stability. As an example of using molecular modeling to create experimentally testable hypotheses, we were able to suggest a new design based on pyrrylene vinylene monomers. In another project, we combined experiments and molecular modeling to gain insight into factors that influence the kinetic binding dynamics of fibrin "knob" peptides and complementary "holes." Molecular dynamics simulations provided helpful information about potential peptide structural conformations and intrachain interactions that may influence binding properties. The remaining projects discussed in this thesis all deal with RNA structure. The underlying approach for these studies is a recently developed chemical probing technology called 2'-hydroxyl acylation analyzed by primer extension (SHAPE). One study focuses on ribosomal RNA, specifically the 23S rRNA from T. thermophilus. We used SHAPE experiments to show that Domain III of the T. thermophilus 23S rRNA is an independently folding domain. This first required the development of our own data processing program for generating quantitative and interpretable data from our SHAPE experiments, due to limitations of existing programs and modifications to the experimental protocol. In another study, we used SHAPE chemistry to study the in vitro transcript of the RNA genome of satellite tobacco mosaic virus (STMV). This involved incorporating the SHAPE data into a secondary structure prediction program. The SHAPE-directed secondary structure of the STMV RNA was highly extended and considerably different from that proposed for the RNA in the intact virion. Finally, analyzing SHAPE data requires navigating a complex data processing pipeline. We review some of the various ways of running a SHAPE experiment, and how this affects the approach to data analysis.
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Discovery and identification of bioactive components by molecular docking.January 2013 (has links)
隨著個人計算機運算能力的快速發展,虛擬藥物篩檢已被廣泛運用。目前運用於計算機輔助藥物虛擬篩選的化合物數據庫多為人工合成的數據庫,而用於天然產物藥物篩選的數據庫則較少報道。為了加速天然化合物的虛擬篩選,我們建立了包含約8000個天然產物的數據庫。他們中的大多數為傳統中藥。 / 為了驗證天然產物數據庫的可用性,其被用於篩選乙酰膽鹼酯酶抑製劑。該數據庫成功地確定了美國藥品監督管理局所批準的乙酰膽鹼酯酶抑製劑,如石杉鹼甲和他克林,表示該天然產物數據庫可以用於藥物虛擬對接篩選。 / 除了已知的乙酰膽鹼酯酶抑製劑,十二種植物化學物(大黃酸,大黃素,蘆薈大黃素,大黃酚,花椒毒素,珊瑚菜素,別異歐前胡素,歐前胡素,紫草素,乙酰紫草素,異戊紫草素和β,β-二甲基丙烯酰紫草素)被確定為新的乙酰膽鹼酯酶抑製劑。澱粉樣蛋白聚集可以導致神經細胞死亡;本研究中新發現的乙酰膽鹼酯酶抑製劑乙酰紫草素能夠阻止澱粉樣蛋白的聚集。除此之外,乙酰紫草素及其衍生物可以對抗過氧化氫誘導的神經細胞凋亡。其抗凋亡的活性作用是通過抑制活性氧的產生,以及保護線粒體膜電位的損失所實現的。亞鐵血紅素加氧酶在其神經細胞保護作用中起重要作用。 / 趨化因子受體4為跨膜G蛋白偶聯受體(GPCRs)。 CXCR4已被確定為一個新治療以及預防腫瘤轉移的新靶點。本研究利用分子對接篩選,從天然產物數據庫篩選選出CXCR4拮抗劑。通過分子對接和基於細胞的測定,黃芪甲苷,羥基紅花黃色素A和水飛薊賓已被確定為CXCR4拮抗劑。抗轉移的研究表明,黃芪甲苷和水飛薊賓抑制CXCL12誘導乳腺癌細胞的遷移和侵襲。此外,水飛薊賓也抑制CXCL12誘導的人臍靜脈內皮細胞管形成。另一方面,羥基紅花黃色素A對乳腺癌細胞的增殖表現出較強的抑製作用,因此很難進行抗轉移實驗。 / With the rapid advances in personal computing power, virtual drug screening has become increasingly popular. While there are numerous databases for synthetic compounds, there are few natural product databases that are specifically for in silico docking studies. To facilitate virtual docking on natural compounds, in-house Natural Products Database has been established, which contains approximately 8,000 naturally occurring chemicals so far. Most of them are documented Traditional Chinese Medicines. / In order to validate the usefulness of the database, in silico screening of acetylcholinesterase inhibitors (AChEIs) by virtual docking was performed. The database successfully identified the FDA-approved AChEIs such as huperzine and tacrine, indicating the in-house database is workable for natural products docking screening. / Apart from well-known AChE inhibitors, twelve phytochemicals (emodin, aloe-emodin, chrysophanol, rhein, xanthotoxin, phellopterin, alloisoimperatorin, imperatorin, shikonin, acetylshikonin, isovalerylshikonin and β, β-dimethylacrylshikonin) were identified as AChE inhibitors in this study that were not previously reported. Amyloid aggregation leads to toxic species that cause neuronal cell deaths, it was found that the newly identified AChEIs acetylshikonin and shikonin are able to prevent amyloid aggregation. A series of cell-based analysis were conducted for in vitro evaluation of the neuroprotective activities of the newly identified AChEIs. Acetylshikonin and its derivatives was found to prevent apoptotic cell death induced by hydrogen peroxide in human and rat neuronal SH-SY5Y and PC12 cells at 10 μM. Acetylshikonin exhibited the most potent anti-apoptotic activity through inhibition of reactive oxygen species (ROS) generation as well as protection of the loss of mitochondria membrane potential. Furthermore, acetylshikonin upregulates hemooxygenase 1(HO-1) which is a key step mediating its anti-apoptotic activity from oxidative stress in SH-SY5Y cells. / The C-X-C chemokine receptor type 4 (CXCR4) belongs to the class A family of seven transmembrane G protein-coupled receptors (GPCRs). CXCR4 has been identified as one of novel target against metastasis. A search for natural CXCR4 antagonists was conducted from natural product database by molecular docking for anti-metastasis study. Astragaloside IV, hydroxy safflower yellow A and silibinin have been identified as novel CXCR4 antagonists by both molecular docking and characterized by various cell-based assays. Anti-metastasis study showed that astragaloside IV and silibinin inhibited CXCL12-induced migration and invasion in breast cancer cells. In addition, silibinin also inhibited CXCL12-induced tube formation in human umbilical vein endothelial cells. On the other hand, hydroxy safflower yellow A exhibited a strong cytotoxicity on breast cancer cell proliferation, which is difficult to conduct anti-metastasis experiments. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Detailed summary in vernacular field only. / Wang, Yan. / Thesis (Ph.D.) Chinese University of Hong Kong, 2013. / Includes bibliographical references (leaves 230-250). / Abstracts also in Chinese.
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Rasterkraftmikroskopie an weichen MaterialienGörlich, Martin 15 January 2004 (has links)
In dieser Arbeit wurde an einer Auswahl von Biomolekülen gezeigt, dass die Rasterkraftmikroskopie, welche ursprünglich in physikalischen Labors entwickelt und zunächst auch nur dort eingesetzt wurde, ein äußerst wichtiges Instrument innerhalb der biologischen und medizinischen Forschung darstellt. Es wurde gezeigt, welche Ergebnisse bei der Visualisierung von Biomolekülen erwartet werden können. Die nach einer eigens entwickelten Methode präparierten Actin-Proben konnten hochaufgelöst abgebildet werden. Es zeigten sich neben freiliegenden Einzelfilamenten auch Bündel, welche sich aus Einzelfilamenten zusammensetzen. Es ist ein Modell erstellt worden, welches die unterschiedlichen Breiten der Bündel erklärt. Mit Hilfe der Mikrokontakt-Druck-Technik (microcontact printing, µCP) ist es gelungen, Proteinstrukturen in der Größe weniger Nanometer großflächig auf verschiedene Substrate zu übertragen. Ebenso wurde ein proteophober SAM (self assembling monolayer) gestempelt, mit dem Proteinadsorptionen gezielt unterbunden werden konnten. Das Adsorptionsverhalten des Proteins F1-ATPase auf Gold ist in situ untersucht worden. Parallel dazu wurde die Adsorption des Proteins in einer Mikroquarzwaage (quarz crystal microbalance, QCM) untersucht. Beide Techniken zeigen unterschiedliche Adsorptions-kinetiken, die Kombination beider Methoden lässt jedoch eine Abschätzung der wahren Adsorptionskinetik zu. Ein neuer Messmodus (constant damping mode) ist ob seiner Fähigkeit, biologische Objekte abbilden zu können, geprüft worden. Vergleichsmessungen im Kontakt-, Nichtkontakt- und Dämpfungs-Modus an einer Proteinmultischicht zeigten, dass im Nichtkontakt-Modus die höchste Auflösung erzielt werden kann. Ferner sind Magnetkraftmikroskop-Experimente an dem als Eisenspeicher bekannten Protein Ferritin durchgeführt worden. An einer Submonolage Ferritin auf Glimmer lässt sich jedoch mit dieser Technik kein magnetisches Signal messen.
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