Thesis (Ph. D.)--Lehigh University, 1997. / Includes vita. Bibliography: leaves 203-219.

M. Tech. Biotechnology. / Enterobacter sakazakii is an emerging pathogen implicated in serious illness and death in infants associated with consumption of contaminated powdered infant formula. Due to the HIV/AIDS pandemic and an increase in working mothers, the consumption of powdered infant formula is increasing. Unfortunately, isolation and identification methods for E. sakazakii have not yet been validated and standardized. Therefore, five isolation and identification methods for E. sakazakii from literature were evaluated. Subsequently the superior method was used for screening powdered infant formula.and other dairy products from the South African market for the prevalence of E. sakazakii and other Enterobacteriaceae bacteria.

Thesis (doctoral)--Lund University, 1997.

Thesis (doctoral)--Lund University, 1997.

In this dissertation a computer-aided automation design methodology for biotechnology applications is proposed that leads to several design guidelines. Because of the biological nature of the samples that propagate in the automation line, a very specific set of environmental and maximum allowed shelf time conditions have to be followed to obtain good yield. In addition all biotechnology protocols require precise sequence of steps, the samples are scarce and the reagents are costly, so no waste can be afforded. / Includes bibliography. / Dissertation (Ph.D.)--Florida Atlantic University, 2014. / FAU Electronic Theses and Dissertations Collection

Thesis (M.Tech.:Biotechnology)-Dept. of Biotechnology, Durban University of Technology, 2007 xvi, 185 leaves / Spirulina is a blue-green, multicellular, filamentous cyanobacterium that can grow to sizes of 0.5 millimetres in length. It is an obligate photoautotroph and has a pH growth range from 8.3 to 11.0.The large-scale production of Spirulina biomass depends on many factors, the most important of which are nutrient availability, temperature and light. These factors can influence the growth of Spirulina and the composition of the biomass produced by changes in metabolism. Brine effluent from cooling towers of electricity generating plants may provide an ideal growth medium for Spirulina based on its growth requirements, i.e. high alkalinity and salinity. The aim of this research was to optimise brine effluent from cooling towers by supplementing it with salts, in order to use this optimised effluent in a small open laboratory raceway pond in an attempt to increase the biomass production of Spirulina.

Thesis (M.Tech.:Biotechnology)-Dept. of Biotechnology, Durban University of Technology, 2007 xvi, 185 leaves / Spirulina is a blue-green, multicellular, filamentous cyanobacterium that can grow to sizes of 0.5 millimetres in length. It is an obligate photoautotroph and has a pH growth range from 8.3 to 11.0.The large-scale production of Spirulina biomass depends on many factors, the most important of which are nutrient availability, temperature and light. These factors can influence the growth of Spirulina and the composition of the biomass produced by changes in metabolism. Brine effluent from cooling towers of electricity generating plants may provide an ideal growth medium for Spirulina based on its growth requirements, i.e. high alkalinity and salinity. The aim of this research was to optimise brine effluent from cooling towers by supplementing it with salts, in order to use this optimised effluent in a small open laboratory raceway pond in an attempt to increase the biomass production of Spirulina.

Thesis (PhD)--University of Stellenbosch, 2004. / 271 Leaves printed single pages, preliminary pages i-xviii and 253 numberd pages. Includes bibliography. List of figures, List of tables. / ENGLISH ABSTRACT: A Neurocontrol Paradigm for Intelligent Process Control using Evolutionary Reinforcement Learning Balancing multiple business and operational objectives within a comprehensive control strategy is a complex configuration task. Non-linearities and complex multiple process interactions combine as formidable cause-effect interrelationships. A clear understanding of these relationships is often instrumental to meeting the process control objectives. However, such control system configurations are generally conceived in a qualitative manner and with pronounced reliance on past effective configurations (Foss, 1973). Thirty years after Foss' critique, control system configuration remains a largely heuristic affair. Biological methods of processing information are fundamentally different from the methods used in conventional control techniques. Biological neural mechanisms (i.e., intelligent systems) are based on partial models, largely devoid of the system's underlying natural laws. Neural control strategies are carried out without a pure mathematical formulation of the task or the environment. Rather, biological systems rely on knowledge of cause-effect interactions, creating robust control strategies from ill-defined dynamic systems. Dynamic modelling may be either phenomenological or empirical. Phenomenological models are derived from first principles and typically consist of algebraic and differential equations. First principles modelling is both time consuming and expensive. Vast data warehouses of historical plant data make empirical modelling attractive. Singular spectrum analysis (SSA) is a rapid model development technique for identifying dominant state variables from historical plant time series data. Since time series data invariably covers a limited region of the state space, SSA models are almost necessarily partial models. Interpreting and learning causal relationships from dynamic models requires sufficient feedback of the environment's state. Systemisation of the learning task is imperative. Reinforcement learning is a computational approach to understanding and automating goal-directed learning. This thesis aimed to establish a neurocontrol paradigm for non-linear, high dimensional processes within an evolutionary reinforcement learning (ERL) framework. Symbiotic memetic neuro-evolution (SMNE) is an ERL algorithm developed for global tuning of neurocontroller weights. SMNE is comprised of a symbiotic evolutionary algorithm and local particle swarm optimisation. Implicit fitness sharing ensures a global search and the synergy between global and local search speeds convergence.Several simulation studies have been undertaken, viz. a highly non-linear bioreactor, a rigorous ball mill grinding circuit and the Tennessee Eastman control challenge. Pseudo-empirical modelling of an industrial fed-batch fermentation shows the application of SSA for developing partial models. Using SSA, state estimation is forthcoming without resorting to fundamental models. A dynamic model of a multieffect batch distillation (MEBAD) pilot plant was fashioned using SSA. Thereafter, SMNE developed a neurocontroller for on-line implementation using the SSA model of the MEBAD pilot plant. Both simulated and experimental studies confirmed the robust performance of ERL neurocontrollers. Coordinated flow sheet design, steady state optimisation and nonlinear controller development encompass a comprehensive methodology. Effective selection of controlled variables and pairing of process and manipulated variables were implicit to the SMNE methodology. High economic performance was attained in highly non-linear regions of the state space. SMNE imparted significant generalisation in the face of process uncertainty. Nevertheless, changing process conditions may necessitate neurocontroller adaptation. Adaptive neural swarming (ANS) allows for adaptation to drifting process conditions and tracking of the economic optimum online. Additionally, SMNE allows for control strategy design beyond single unit operations. SMNE is equally applicable to processes with high dimensionality, developing plant-wide control strategies. Many of the difficulties in conventional plant-wide control may be circumvented in the biologically motivated approach of the SMNE algorithm. Future work will focus on refinements to both SMNE and SSA. SMNE and SSA thus offer a non-heuristic, quantitative approach that requires minimal engineering judgement or knowledge, making the methodology free of subjective design input. Evolutionary reinforcement learning offers significant advantages for developing high performance control strategies for the chemical, mineral and metallurgical industries. Symbiotic memetic neuro-evolution (SMNE), adaptive neural swarming (ANS) and singular spectrum analysis (SSA) present a response to Foss' critique. / AFRIKAANSE OPSOMMING: 'n Neurobeheer paradigma vir intelligente prosesbeheer deur die gebruik van evolusionêre versterkingsleer Dit is 'n komplekse ontwikkelingstaak om menigte besigheids- en operasionele doelwitte in 'n omvattende beheerstrategie te vereenselwig. Nie-lineêriteite en vele komplekse prosesinteraksies kombineer om ingewikkelde aksie-reaksie verwantskappe te vorm. Dit is dikwels noodsaaklik om hierdie interaksies omvattend te verstaan, voordat prosesbeheer doelwitte doeltreffend gedoen kan word. Tog word sulke beheerstelsels dikwels saamgestel op grond van kwalitatiewe kriteria en word ook dikwels staatgemaak op historiese benaderings wat voorheen effektief was (Foss, 1973). Dertig jaar na Foss se kritiek, bly prosesbeheerstelsel ontwerp 'n heuristiese saak. Die biologiese prosessering van informasie is fundamenteel verskillend van metodes wat gebruik word in konvensionele beheertegnieke. Biologiese neurale meganismes (d.w.s., intelligente stelsels) word gebaseer op gedeeltelike modelle, wat grotendeels verwyderd is van die onderskrywende natuurwette. Neurobeheerstrategieë word toegepas sonder suiwer wiskundige formulering van die taak of die omgewing. Biologiese stelsels maak eerder staat op kennis van aksie-reaksie verhoudings en skep robuuste beheerstrategieë van swak gedefineerde dinamiese stelsels. Dinamiese modelle is of fundamenteel of empiries. Fundamentele modelle word ontwikkel vanaf eerste beginsels en word tipies uit algebraïese en differensiële vergelykings saamgestel. Modellering vanaf eerste beginsels is beide tydrowend en duur. Groot databasisse van historiese aanlegdata maak empiriese modellering aantreklik. Singuliere spektrumanalise (SSA) maak die vinnige ontwerp van empiriese modelle moontlik, waardeur dominante veranderlikes vanaf historiese tydreekse onttrek kan word. Aangesien tydreeksdata slegs 'n gedeelte van die prosesomgewing verteenwoordig, is SSA modelle noodwendig gedeeltelike modelle. Die interpretasie en aanleer van kousale verhoudings vanaf dinamiese modelle vereis voldoende terugvoer van omgewingstoestande. Die leertaak moet sistematies uitgevoer word. Versterkingsleer is 'n ramingsbenadering tot 'n doelwit-gedrewe leerproses. Hierdie tesis bewerkstellig 'n neurobeheerparadigme vir nie-lineêre prosesse met hoë dimensies binne 'n evolusionêre versterkingsleer (EVL) raamwerk. Simbiotiese, memetiese neuro-evolusie (SMNE) is 'n EVL algoritme wat ontwikkel is vir globale verstelling van die gewigte van ‘n neurobeheerder. SMNE is saamgestel uit 'n simbiotiese evolusionêre algoritme en 'n lokale partikelswerm-algoritme. Implisiete fiksheidsdeling verseker 'n globale soektog en die sinergie tussen globale en lokale soektogte bespoedig konvergensie.Verskeie simulasie studies is onderneem, o.a. die van 'n hoogs nie-lineêre bioreaktor, 'n balmeulaanleg en die Tennessee Eastman beheer probleem. Empiriese modellering van 'n industriële enkelladingsfermentasie demonstreer die aanwending van SSA vir die ontwikkeling van gedeeltelike modelle. SSA benader die toestand van 'n dinamiese stelsel sonder die aanwending van fundamentele modellering. 'n Dinamiese model van 'n multi-effek-enkelladingsdistillasie (MEBAD) proefaanleg is bewerkstellig deur die gebruik van SSA. Daarna is SMNE gebruik om 'n neurobeheerder te skep vanaf die SSA model vir die beheer van die MEBAD proefaanleg. Beide simulasie en eksperimentele studies het die robuuste aanwending van EVL neurobeheerders bevestig. Die gekoördineerde ontwerp van vloeidiagramme, gestadigde toestand-optimering en nie-lineêre beheerderontwikkeling vereis 'n omvattende metodologie. Beheerveranderlikes en die koppeling van proses- en uitvoerveranderlikes is implisiet en effektief. Maksimale ekonomiese aanwins was moontlik in hoogs nie-lineêre dele van die toestandsruimte. SMNE het besondere veralgemening toegevoeg tot neurobeheerderstrategieë ten spyte van prosesonsekerhede. Nietemin, veranderende prosestoestande mag neurobeheerderaanpassing genoodsaak. Aanpasbare neurale swerm (ANS) algoritmes pas neurobeheerders aan tydens veranderende proseskondisies en volg die ekonomiese optimum, terwyl die beheerder die proses beheer. SMNE bewerkstellig ook die ontwikkeling van beheerstrategieë vir prosesse met meer as een eenheidsoperasie. SMNE skaal na prosesse met hoë dimensionaliteit vir die ontwikkeling van aanlegwye beheerstrategieë. Talle kwelvrae in konvensionele aanleg-wye prosesbeheer word deur die biologies gemotiveerde benadering van die SMNE algoritme uit die weg geruim. Toekomstige werk sal fokus op die verfyning van beide SMNE en SSA. SMNE en SSA bied 'n nie-heuristiese, kwantitatiewe benadering wat minimale ingenieurskennis of oordeel vereis. Die metodologie is dus vry van subjektiewe ontwerpsoordeel. Evolusionêre versterkingsleer bied talle voordele vir 'n ontwikkeling van effektiewe beheerstrategieë vir die chemiese, mineraal en metallurgiese industrieë. Simbiotiese memetiese neuro-evolusie (SMNE), aanpasbare neurale swerm metodes (ANS) en singulêre spektrum analise (SSA) gee antwoord op Foss se kritiek.

The exponential development of biotechnology has lead to a quasi unlimited number of potential products going from biopolymers to vaccines. Cell culture has therefore evolved from the simple cell growth outside its natural environment to its use to produce molecules that they do not naturally produce. This rapid development could not be continued without new control and supervising tools as well as a good process understanding. This requirement involves however a large diversity and a better accessibility of process measurements. In this framework, software sensors show numerous potentialities. The objective of a software sensor is indeed to provide an estimation of the system state variables and particularly those which are not obtained through in situ hardware sensors or laborious and expensive analysis. In this context, This work attempts to join the knowledge of increasing bioprocess complexity and diversity and the time scale of process developments and favours systematic modelling methodology, its flexibility and the speed of development. In the field of state observation, an important modelling constraint is the one induced by the selection of the state to estimate and the available measurements. Another important constraint is the model quality. The central axe of this work is to provide solutions in order to reduce the weight of these constraints to software sensors development. On this purpose, we propose four solutions to four main questions that may arise. The first two ones concern modelling uncertainties.<p><p>1."How to develop a software sensor using measurements easily available on pilot scale bioreactor?" The proposed solution is a static software sensor using an artificial neural network. Following this modelling methodology we developed static software sensors for the biomass and ethanol concentrations in a pilot scale S. cerevisae cell culture using the measurement of titrating base quantity, agitation rate and CO& / Doctorat en sciences agronomiques et ingénierie biologique / info:eu-repo/semantics/nonPublished