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Mining a Chinese hyperthermophilic metagenome/Du Plessis, Morne Graham. Unknown Date (has links) (PDF)
Thesis (Ph. D) -- University of the Western Cape, 2007. / Includes bibliographic references , (186-222).
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Exploring the potential of algae-bacteria communities for biotechnologyRiseley, Anthony Shawn January 2018 (has links)
Microalgae are a large and diverse group of photosynthetic organisms ranging from prokaryotic cyanobacteria to eukaryotic algae spread across many phyla. Traditional algal biotechnology approaches have focused on growing algae in monoculture, in contrast to nature, where algae live in association with many other organisms. One association of interest is between the bacterium Mesorhizobium loti (Rhizobiales) and the green alga, Lobomonas rostrata deficient in the production of vitamin B12. The alga provides fixed carbon to the bacterium whereas the bacterium supplies vitamin B12 to the alga. In the course of a screen for bacterial mutants altered in the interaction, a novel symbiosis was serendipitously identified involving the non-Rhizobiales bacterium Rhodococcus erythropolis and L. rostrata. This novel interaction, together with interaction of the more industrially relevant Chlamydomonas reinhardtii strain was characterized. Nitrogen is a major limiting nutrient for industrial scale algal production. An alternative option to the Haber-Bosch process of synthesising and supplementing fixed nitrogen into media is to utilise nitrogen fixing bacteria capable of secreting fixed nitrogen into the media otherwise known as biofertilisation. Anabaena sp. PCC 7120 is a filamentous cyanobacteria that can fix its own nitrogen and engineered strains capable of releasing fixed nitrogen in the form of amino acids and ammonium were cultured with the industrially relevant Chlamydomonas reinhardtii metE—+ M. loti consortium and Chlorella vulgaris in nitrogen-free media. There are relatively few published studies investigating and outlining the challenges involved in scaling algae production from the laboratory through to pilot scale. Furthermore, these studies have typically focused on growing axenic cultures. The B12-dependent strain of C. reinhardtii metE— was grown in the presence of supplemented B12 and B12 producing M. loti at lab scale (50 mL), pre-pilot scale (10 L) and pilot scale (60 L). The growth efficiency as determined by growth rate, was measured and compared for both cultures at all scales.
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IMPLICAÇÕES JURÍDICAS NA UTILIZAÇÃO DE ORGANISMOS GENETICAMENTE MODIFICADOS: OS ALIMENTOS TRANSGÊNICOS.Mello, Cecy Pereira Figueira da Silva Neta 14 March 2016 (has links)
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Previous issue date: 2016-03-14 / With the biotechnology advancement and development, with special focus on genetic
engineering, also rises the requirement of ethical fundamentals in a scenario of
constant modifications. When it comes to transgenic food, there‟s controversy in the
philosophical, ethical, environmental, political, legal and economic field because it‟s a
subject which is inside of the daily reality, with very fast changes and in that context,
the Brazilian and international legislation seems to doesn‟t follow the technology
velocity and Science becomes a political and economical instrument. In this article,
international law and national law related to the using of genetically modified
organism are analyzed, pointing out the 11.105/05 law, considering the origin, shape
and evolution of genetically modified organism, the importance of obeying the
environmental law when handling and distributing products resulting of these genetic
modifications to the public consumption, this law analysis, going through the
environmental impacts, giving importance to the cost-benefit and the risk expressly
taken by the scientific community, besides the own concept of consumer, proving the
need of genetically modified food labelling and the reality of what really happens in
the process of patenting acquisition in the international law and in the Brazilian law
and jurisprudence decisions. / Com o avanço e o desenvolvimento da biotecnologia, com enfoque especial na
engenharia genética, surge também a necessidade da inserção da ética e de seus
princípios em um cenário de modificações constantes. No que tange aos alimentos
transgênicos, polêmicas são levantadas no campo filosófico, ético, ambiental,
político, social, jurídico e econômico, pois o tema está inserido em uma realidade do
nosso dia a dia, com mudanças muito rápidas e diante deste contexto, as
legislações brasileiras e internacionais parecem não acompanhar a velocidade da
tecnologia e a ciência passa a ser instrumento da política e da economia. No
presente trabalho são analisadas leis internacionais, legislações relativas à utilização
dos organismos geneticamente modificados, ressaltando-se a Lei 11.105/05,
considerando a origem, a forma e a evolução do organismo geneticamente
modificado, a importância da obediência às leis de Direito Ambiental no manuseio e
distribuição dos produtos oriundos destas modificações genéticas para o consumo
da população, a análise desta legislação, passando pelos impactos ambientais,
dando ênfase ao custo benefício e ao risco assumido expressamente pela
comunidade científica, discorrendo ainda sobre o direito do consumidor, além do
próprio conceito de consumidor, demonstrando também a necessidade da rotulagem
dos alimentos geneticamente modificados e a forma como acontece o processo para
obtenção de patentes tanto na legislação exterior quanto na legislação brasileira e
as decisões jurisprudenciais.
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