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Characterising microsatellite loci in the blue crane (Grus paradisea)Meares, Kathleen Frances. January 2007 (has links)
The blue crane (Grus paradisea) is endemic to southern Africa and has the smallest
geographical range of the 15 crane species occurring world-wide. Although this species is still
found throughout most of its historic range, it has experienced a significant and rapid decline in
numbers over the last 20 years. One factor causing this decline is the illegal removal of chicks
from the wild. Permits are required to keep, trade in and breed cranes in captivity. However,
birds must be captive bred in order to obtain a permit. Therefore, chicks taken illegally from the
wild are fraudulently incorporated into an existing captive population under the pretence that
they offspring of a legal captive pair. This study describes the development of a set of
microsatellite markers to assist the identification of illegal trade in the blue crane. These
markers can ultimately be used to verify the relationship between the offspring and its claimed
parents by performing parentage analyses. Forty microsatellite loci were obtained from
genomic libraries previously developed in two other crane species and tested for cross-species
utility in the blue crane. In addition, 42 loci were developed for this study from a blue crane
species-specific genomic microsatellite library, of which 19 were tested for polymorphism in
this species. The microsatellite markers characterised here were also tested for their utility in
two other crane species: wattled crane (G. carunculatus) and grey-crowned crane (Balearica
regulorum). One locus, Gamu007, was found to be sex-linked and therefore excluded from the
set of markers. A total of 28 polymorphic loci were tested for the suitability in parentage
analysis in the blue crane. Of these, a set of 16 loci were determined to be as suitable for this
purpose. These loci were shown to be inherited in a Mendelian fashion in a single blue crane
family. In addition, statistical analysis of the loci were identified as exhibiting linkage
equilibrium, this was supported by their distant association on a predicted Grus microsatellite
map based on the chicken genome. The selected loci were also identified as having a low
frequency of null alleles as well as a total first and second parent exclusion power of 0.9999
and 1.0000, respectively. These loci provide a valuable tool for parentage testing in blue crane,
and may also be valuable in population genetic studies to assist conservation strategies. In
addition, this set may be used to assist legal cases involving the illegal trade in blue cranes
upon completion of additional microsatellite marker validation procedures. Twenty-seven loci
were polymorphic in the wattled and grey-crowned crane. These could provide a valuable
source of micro satellite loci in these species, and could potentially eliminate the need for the
development of a species-specific microsatellite library. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2007.
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Wind Energy-related Wildlife Impacts: Analysis and Potential Implications for Rare, Threatened and Endangered Species of Birds and Bats in TexasGraham, Tara L. 08 1900 (has links)
Texas currently maintains the highest installed nameplate capacity and does not require publicly available post-construction monitoring studies that examine the impacts of wind energy production on surrounding fauna. This thesis examines potential wind energy impacts on avian and bat species in Texas through a three-part objective. The first two objectives synthesize literature on variables attractive to species within wind development areas and estimate impacted ranges outside of Texas, based on studies examining wind energy's environmental impacts. The third objective focuses on Texas wind development potential for interaction with rare, threatened and endangered species of birds and bats using GIS analysis with a potential hazard index (PHI) model, which addresses broad-spectrum, high risk variables examined within the first two objectives. Assuming areas with higher wind speeds have potential for wind development, PHI values were calculated for 31 avian and ten bat species, based on an analysis of species range data obtained from the Texas Parks and Wildlife Department and wind data obtained from the National Renewable Energy Laboratory. Results indicate one avian species, Tympanuchus pallidicinctus, is at high risk for wind development interaction on an annual basis, with 20 species of birds and nine species of bats at higher risk during the spring season. This macro-scale approach for identifying high risk species in Texas could be used as a model to apply to other conterminous states' preliminary evaluation of wind energy impacts.
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Ray P. Holland, Editor and ConservationistHolland, Linda R. 05 1900 (has links)
The problem involved in this study was to determine the success or failure of the use of the editorial in achieving a goal, specifically, editorials by Ray P. Holland in favor of the Migratory Bird Conservation Act of 1929 and the Migratory Bird Hunting Stamp Act of 1934. Editorials from the Bulletin of the American Game Protective Association and Field and Stream were classified into categories to show an editorial position over a selected time period. Material from the permanent collection of the Holland library at Wesleyan University and family information was used to record a biography of Holland's achievements. The study shows that Holland's contributions in journalism will stand as a benchmark to ensuing generations of journalists who seek to realize their goals through the proper use of the editorial.
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Caractérisation de l’hétérogénéité spatiale de milieux naturels à partir d’imagerie optique très haute résolution spatiale : cas d’application aux milieux méditerranéens de garrigue / Characterization of the spatial heterogeneity of natural environments from very high spatial resolution optical imagery : an application case to garrigue Mediterranean habitatsLang, Marc 29 May 2019 (has links)
La préservation de la biodiversité est un enjeu prioritaire, identifié aussi bien au niveau national qu’au niveau européen et international. L’hétérogénéité spatiale des milieux naturels est l’une des composantes clefs pour l’étude de la biodiversité et permet de comprendre le fonctionnement des écosystèmes. Le bassin Méditerranéen est un hotspot de biodiversité pour lequel le lien entre biodiversité et hétérogénéité spatiale des paysages s’illustre particulièrement bien. Les milieux méditerranéens s'organisent en mosaïques hétérogènes de quatre strates verticales: le sol nu, l’herbe, les ligneux bas et les ligneux hauts. La biodiversité de ces milieux est aujourd'hui menacée par une fermeture de milieux naturels qui entraîne la disparition de certains habitats et l’homogénéisation des paysages, homogénéisation qui entraîne elle-même une augmentation des risques d’incendies.Cette thèse se propose de développer des indices caractérisant l’hétérogénéité spatiale des milieux naturels dans un contexte méditerranéen à partir d’images de télédétection à très haute résolution. Parmi les différentes méthodes permettant de caractériser l'hétérogénéité, la méthode FOTO (FOurier Based Textural Ordination) est particulièrement intéressante car elle produit de façon non supervisée un nombre limité de gradients de texture non corrélés, à partir desquels il est possible de décrire les variations continues de l’hétérogénéité spatiale, et ce, à plusieurs échelles spatiales. Ainsi le premier objectif de cette thèse est de tester le potentiel des gradients de texture issus de la méthode FOTO pour la caractérisation de l’hétérogénéité spatiale relative aux quatre strates verticales caractéristiques des milieux méditerranéens. Le deuxième objectif est de tester la sensibilité de l’approche développée à des facteurs techniques et environnementaux, afin de s’assurer de sa réplicabilité pour favoriser son utilisation dans un contexte opérationnel de suivi des milieux méditerranéens. Enfin, le dernier objectif est de valider la pertinence écologique des indices d’hétérogénéité développés à travers un cas d’application~: la caractérisation de la répartition spatiale d’espèces d’oiseaux sensibles à l’hétérogénéité de la végétation.Combinés avec un indice de végétation, le NDVI, les indices de texture issus de la méthode FOTO ont pu être interprétés en termes d'hétérogénéité spatiale et ont permis de caractériser la composition et l'organisation des quatre strates verticales étudiées. Ces indices sont influencés par la présence de surfaces anthropisées comme les cultures ainsi que par la nature de l'information radiométrique des images de télédétection utilisées, qui impacte le contraste apparent des strates de végétation. Ainsi, l'application de la méthode sur une bande panchromatique est plus sensible aux motifs liés à l'alternance de sol nu et d'herbe tandis que l'application de la méthode sur le NDVI est plus sensible aux motifs lié à l'alternance des ligneux avec la strate herbacée. Enfin, nous avons montré l’intérêt de l’approche développée pour la prédiction de plusieurs espèces d’oiseaux à fort enjeux de conservation. Les indices d’hétérogénéité ont permis de mettre en évidence des structures de la végétation particulièrement favorables à certaines espèces d’oiseaux.L'approche développée dans cette thèse est particulièrement intéressante car elle permet la production non supervisée de trois indices complémentaires caractérisant plusieurs composantes de l'hétérogénéité spatiale relatives à quatre strates. Des efforts sont encore nécessaires pour améliorer i) notre compréhension de la contribution de facteurs environnementaux et instrumentaux sur la stabilité de l'approche et ii) son automatisation en vue d'une application dans un contexte opérationnel pour la cartographie et le suivi de l'état de conservation des habitats naturels et de l'avifaune. / The preservation of biodiversity is a priority issue, both at national, European and international levels. In order to provide a better understanding of ecosystem functioning, spatial heterogeneity of natural environments is becoming one of the key components for the study of biodiversity. The Mediterranean basin is a hotspot of biodiversity for which the synergies between biodiversity and spatial heterogeneity of landscapes are particularly important. Mediterranean environments are organized into heterogeneous mosaics of four vertical strata~: bare soil, herbs, low ligneous and high ligneous. The biodiversity of these unique hotspots is now threatened by a closure of the landscape that leads to the habitat loss and landscape homogenization. The loss of heterogeneity is also leading to an increase in fire risks.This thesis aims to develop indices characterizing the spatial heterogeneity of natural landscapes in a Mediterranean context using very high spatial resolution remote sensing images. Among the various methods dedicated to the characterization of heterogeneity, the FOTO (FOurier Based Textural Ordination) method is particularly relevant because it produces uncorrelated texture gradients in an unsupervised manner, allowing continuous variations in spatial heterogeneity to be characterized at different spatial scales. Thus, the first objective of this thesis is to test the potential of texture indices derived from the FOTO method for the characterization of spatial heterogeneity relative to four vertical strata. The second objective is to test the sensitivity of our approach to technical and environmental factors, in order to ensure its replicability, and promote its use in an operational context of monitoring Mediterranean environments. Finally, based on a case study centered on the spatial distribution of bird species sensitive to vegetation heterogeneity, the last objective is oriented towards the validation of the ecological relevance of the heterogeneity indices.Combined with a vegetation index, NDVI, the texture indices derived from the FOTO method could be interpreted in terms of spatial heterogeneity and make it possible to characterize the composition and organization of the four vertical strata studied. These indices are influenced bymultiple factors, including the anthropization of landscapes showing patterns translated into surfaces such as crops, and the nature of the radiometric information of the remote sensing images processed. This pattern information impacts the apparent contrast of vegetation strata. Thus, the application of the method on a panchromatic band is more sensitive to patterns related to the alternation of bare soil and herbs while the application of the method on NDVI is more sensitive to patterns related to the alternation of ligneous strata with the herbaceous stratum. Finally, we have shown the interest of the approach developed for the prediction of several bird species with high conservation stakes. Heterogeneity indices have made it possible to highlight vegetation structures that are particularly favourable to certain bird species.The approach developed in this thesis is particularly stimulating because it allows the unsupervised production of three complementary indices characterizing several components of spatial heterogeneity related to four strata. Further efforts are needed to improve i) our understanding of the contribution of environmental and instrumental factors to the stability of the approach and ii) its automation for application in an operational context for mapping and monitoring the conservation status of natural habitats and birdlife.
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The use of a geographic information system to investigate the effect of land-use change on wattled crane Bugeranus carunculatus breeding productivity in KwaZulu-Natal, South Africa.Coverdale, Brent Miles. January 2006 (has links)
The Wattled Crane, Bugeranus carunculatus Gmelin, is presently classified as being 'Critically Endangered' within South Africa according to the Eskom Red Data book of Birds of South Africa, Lesotho and Swaziland, with a population of a meagre 235 individuals. Of this, 85% occur within KwaZulu-Natal and live predominantly on privately owned agricultural land. As a result thereof, Wattled Cranes and agriculture compete for the same resources. Up until now, the loss of viable habitat, as a result of agricultural development and afforestation, has been mooted as being the primary reason for the decline in numbers of the species. The advancements in the Geographical Information Systems field have enabled conservationists to acquire data, especially pertaining to habitat requirements, which were previously unattainable. This improved data acquisition is enabling for more informed decision making and better allocation of resources. The study therefore attempts to make use of a Geographical Information System to determine whether or not differences exist within the home ranges of active and historical Wattled Crane nesting sites, utilising the National Land cover database. The updated Land cover data for South Africa, although not completed at the time the present study took place, allowed for the interrogation of the various Land cover classes within an estimated home range. Natural Grassland was the predominant Land cover type within both active and historical home ranges, whilst both active and historical home ranges were subject to some degree of transformation. The potential impact of management practices in and around nesting sites warrants further investigation because this could not be determined through the analysis of land cover. / Thesis (M.Env.Dev.)-University of KwaZulu-Natal, Pietermaritzburg, 2006.
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