By specifically analysing for the various active principles of plasma, platelets, tissues and their fractions, much new information has been obtained concerning the role of lipoids and platelets in blood coagulation and in the hemostatic mechanisms in health and disease. Analysed components are studied in artificial clotting systems, especially a two-stage thrombin- forming system. Some 86 cases of bleeding disorders, 32 new born normal infants and their mothers, and many normal adult bloods have been analysed with respect to components of the clotting and hemostatic functions. The detailed considerations embodied in the thesis are encompassed under the following heads: 1) the importance of certain lipoids, especially cephalin 2) the normal need, in plasma clotting, for platelets, 3) the particular significance of a platelet component, which has many analogies to cephalin, in the thromboplastic system, 4) potentiation of the thromboplastic actions of cephalin, of platelets, and of tissue thromboplastin (to some extent) by a variety of experimental additives. Part of this may be explained as a 'thromboplastin generation' through co-participation of certain plasmatic components (antihemophilic globulin, PTC" etc. ) . Part, however, may be the result of certain proteolytic enzymes, particularly trypsin, 'disaggregating' lipoproteins and thus rendering their phospholipid (and sometimes calcium) available for participation in the clotting reactions, 5) possible Ca-containing and lipid-containing 'intermediates’ in the thrombin-forming reactions, 6) myelin figure formation as an explanation of ‘alterations' of platelets and certain other formed elements such as thrombocytes, megakaryocytes, and stromatolytic erythrocytes 7) the multiplicity of factors which platelets may contribute to the blood clotting and hemostatic mechanisms 8) the occurrence of many clinical disorders due to deficiency of platelet functions. Thrombocytopenias denote deficient numbers ('counts' and total bulk in body). Thrombocytopathies are deficiencies of specific platelet components, e . g. thromboplastic factor, accelerator, vasoconstrictor (5-hydroxy tryptamine), or retractor factor. Such deficiencies can be clinically significant even when the platelet count is normal. Bleeding in leukaemias, uremias, etc. may often be accounted for in these terms, 9) the nature and modes of action of heparin and other 'antithromboplastic’ inhibitors, and of some antiproteases, in relation to the mechanisms discussed 10) the ‘cephalin availability theory' of the author, as a useful working hypothesis to explain the importance of the natural thromboplastic phospholipid. Lipid release from platelet, tissue, or possibly plasma sources may very well be the long-obscure 'trigger mechanism' which initiates blood coagulation.

No description available.

A research report submitted to the Faculty of Health Sciences, University of the Witwatersrand, in partial fulfilment of the requirements for the degree of Master of Medicine in the branch of Anaesthesiology Johannesburg, 2019 / Background Anaesthesia frequently involves administration of refrigerated intravenous drugs to patients. Often overlooked, maintenance of the cold chain forms a key component of pharmacovigilance for anaesthetists. However the South African national Department of Health guidelines on: “Cold Chain and Immunisation Operations Manual”, does not detail specific requirements for medically validated cold boxes. Consequently the risk of iatrogenic harm to patients from heat-sensitive pharmaceuticals in inappropriately temperature regulated cold boxes exists. Methods The research design was that of a descriptive, prospective and contextual study. Part I study population comprised the ambient air temperatures of the refrigerator and cold boxes used for storage of heat-sensitive pharmaceuticals in theatre at CHBAH taken at one minute intervals over eight hours. Part II study population was newly purchased cold boxes and cold packs for the purpose of assessing individual cold box thermal performance over eight hours. Results In Part I, only a single cold box (polystyrene box number 19) was able to maintain the recommended temperature range of 2 − 8°C for the eight hour period (4.35%). The refrigerator temperature time plot showed a significant deviation of temperature at approximately 30 minutes. In Part II, only fabric and polystyrene cold boxes with three cold packs in situ were able to maintain the recommended temperature of 2 − 8°C. Conclusion This study highlighted the failure of non-medically validated cold boxes to reliably maintain the temperature of heat-sensitive pharmaceuticals. / E.K. 2019

The investigation was concerned with characterizing effects of this tumor line on lipid metabolism in DBA/1J mice and serum protein levels and cellular changes in DBA/1J and Swiss white mice. Total lipids, lipid phosphorus, neutral lipids, and changes in fatty acids were determined in liver, spleen, skin and tumor of DBA/1J mice bearing the lymphosarcoma at various days after injection of tumor cells.

This work represents the next step in the ongoing development of a system to noninvasively estimate blood perfusion using thermal methods. A combination thermocouple/thermopile sensor records heat flux and temperature measurements on the tissue of interest (in this case skin) for a given period of time. These data, in combination with other experimental parameters, are read into a computer program that compares them to a biothermal finite difference model of the system. The program uses an iterative process incorporating Gauss Minimization to adjust parameters in the biothermal model until the predicted system behavior satisfactorily approximates the real world data. The result is an estimation of blood perfusion in the tissue being measured, as well as an estimate of the thermal contact resistance between the probe and tissue. The system is tested on human forearms, canine legs during laparoscopic spay surgery, and on a canine medial saphenous fasciocutaneous free tissue flap model. Experimental measurements, especially those performed on the tissue flap model, show distinct correlation between blood perfusion and bioprobe output. This research demonstrates the accuracy of the biothermal model and the parameter estimation technique, as well as the usability of the system in a clinical setting. / Master of Science

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No description available.

Potassium, sodium, and calcium are crucial electrolytes of human blood. Among the functions of the electrolytes are maintenance of osmotic pressure and water distribution in the various body fluid compartments, maintenance of the proper pH, regulation of the proper function of the heart and other muscles, involvement in oxidation-reduction or electron transfer reactions, and participation in catalysis as cofactors for enzymes. Therefore, abnormal levels of electrolytes may be either the cause or the consequence of a variety of disorders, and thus the determination of electrolytes in different body fluids in general and in whole human blood particularly is one of the most important functions of the clinical laboratory . Serum or plasma is the usual specimen employed for assay of potassium, sodium, and calcium but not whole human blood. This research intended to create a sensor-array capable of analysing potassium, sodium, and calcium ion-activity in whole human blood based on miniaturised ion-sensors and zero-current direct potentiometry. This research dealt with sensing-side of the sensor-array hoping for integration of these sensors with microelectronics or discrete parts based electronics in due time. Clinics use single electrodes for K+, Na+, and Ca2+ sensing in body fluids. However, single sensing is costly and in emergency cases time consuming as well. Therefore a portable, battery-operated cost- and time-effective sensor array is needed for multi-sensing of r, Na +, and Ca2 + either in emergency cases in field or for patient near bed-side measurement/monitoring. The approach of this thesis to the problem is as follows: This thesis has five phases, all equally important. The first phase, a theoretical phase, mainly deals with the determination of the technique which fits best with chemical sensors and integration with microelectronics, and the decision among many alternative chemicals, whether natural or man-made, available for multi-sensing realisation, by taking into account all the best efforts devoted to development of chemical-sensors. The second phase is the miniaturisation process of the electrochemical sensor-array, either ion-sensors or reference electrode, as much as possible. The third phase is multi-sensing application of the sensorarray for sensing K+, Na+, and ci+ with binary and mixed electrolytes and with artificial and whole human blood respectively, the fourth phase is the sensor-array response with only millilitre or microlitre volumes of whole human blood and the fifth phase is the integration of a sensor array with discrete, parts based, electronic circuitry. So, as a new application a disposable reference electrode has been realised which reasonably fits with the sensor array for the analysis of potassium, sodium, and calcium in whole human blood. Since all the single sensing of these cations have been realised by using a conventional reference electrode, either silver/silver chloride or calomel electrode, which is a bulky and expensive alternative, this research prepared, tested, and implemented a new disposable reference electrode for the sensor-array based on a poly (vinyl alcohol) matrix incorporating a proper amount of Kel. Having a successfully working miniaturised reference electrode, valinomycin, ETH 157, and ETH 1001 ionophores have been used as the selective materials for potassium, sodium, and calcium in whole human blood, respectively. Multisensing applications have only used solutions as testing medium whereas the work described in this thesis goes one step further and uses artificial and whole human blood with fairly reasonable responses.