This study examines spatially continuous sampling data in order to investigate patterns of abundance and distribution of three recreationally important sunfish species (bluegill, redear, and spotted sunfish) along the main-stem of the Peace River, a large softwater river located in southwest Florida. A total of 467 electrofishing transects were sampled biannually from spring 2008 to spring 2010. Sampling sites ranged from the headwaters of the Peace River in Polk County, FL to the oligohaline waters located in Charlotte County, FL. All fish were collected with boat mounted electrofishing gear, and aquatic habitat and physiochemical water quality measurements were recorded at each transect. Pearson's correlation coefficient and conical correspondence analysis were used to interpret relationships between sunfish abundance and associated environmental variables. Results showed that relative abundance of sunfish varied significantly between the upper, middle, and lower basins of the river. Distance from headwaters, conductivity and macrophyte coverage were all significant predictors of relative species abundance. Spotted sunfish were found closer to the headwaters and were positively associated with higher amounts of aquatic vegetation. Redear were most common in the middle basin and were most associated with changes in conductivity. Bluegill were relatively more abundant further downriver where conductivities were higher. Woody debris was strongly associated with fish abundance for all species but did not significantly explain the variance in species composition between locations in the river. The result of this study can be used by fisheries professionals to better manage sunfish populations in the Peace River and other lotic systems.

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The responses at three trophic levels (phytoplankton, zooplankton, young-of-the-year bluegill) were compared between a limed and unlimed arm in a moderately fertile, circumneutral reservoir that was sensitive to acidification, but had not yet shown signs of damage. The east arm (25.5 ha) of Flat Top Lake was treated with 28.8 dry metric tonnes of calcite using slurry box technology between July 13-20, 1987. The design was confounded because calcite dissolution products diffused into the unlimed (west) arm after the treatment which made total alkalinity similar between the arms within 1 month. Phytoplankton gross productivity and chlorophyll a was similar between the arms for the pre- and post-treatment samples as well as for the period after the treatment when total alkalinity was relatively higher in the limed arm. Zooplankton biomass was higher in the unlimed than limed arm in the pre-treatment samples but was similar between the arms in the post-treatments samples; however, the changes in zooplankton biomass after treatment in the limed arm could not be attributed to the treatment. Phytoplankton community composition for the common netplankton and nannoplankton was similar between the limed and unlimed arms for the combined pre- and post-treatment samples: however, Gemellicystis spp., Gloeocystis spp., and Dinobryon spp. showed an order of magnitude higher density in the limed relative to the unlimed arm on the initial post-treatment sample. This difference, as well as the observed post-treatment differences in zooplankton community composition between the two arms could not be attributed to the treatment because there were minimal impacts on nutrient levels (e.g., CO2, total phosphorus) and zooplankton-phytoplankton interactions. Lake transparency was significantly deeper in the limed arm relative to the unlimed arm for the post-treatment samples; however, a corresponding lower dissolved organic carbon concentration was not measured in the limed arm. Young-of-the-year bluegill (e.g., primarily those 15-20 d old) showed significantly higher growth rates in the unlimed arm during the period when total alkalinity was higher in the limed arm, apparently due to a higher density of suitable pelagic zooplankton in the unlimed arm during this period. In conclusion, no significant positive or negative responses to the treatment were detected because of trophic level interactions. The high pre-treatment pH (7.07), small change in post-treatment pH, low dissolution percentage (8-10%), the inability of the sediment dose to neutralize the acidic sediments and induce phosphorus release, and phosphorus rather than carbon dioxide limited primary productivity were the main factors why no significant post-treatment biological responses were detected. / Master of Science

Data were collected from natural and introduced fishes present in Ferndale Lake, a small (120 ha) sport fishing reservoir in Camp County, east Texas. Levels of pH measured in the lake during the study period ranged from 3.5 to 5.3. Monthly field surveys and experimental manipulations were designed to evaluate quantitatively the signs of stress at various biological levels. Lethal limits to low pH were quantified for largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) to be pH 3.8 and 4.0,respectively. Mean blood pH (+ 1 SD) of 59 bluegill was 7.41 (j 0.16), with no significant difference (P-0.05) among groups from Ferndale Lake and Moss Lake (Cooke Co., Texas) under experimental conditions, even when severe stress was externally apparent. In a dual-trough horizontal pH gradient, bluegill behavioral avoidance was observed at pH levels below 7.0. Individual testing of 40 bluegill in pH gradient of 5.2 to 7.6 resulted in median occupation of pH 7.1,with an interquartile range of pH 6.9 to 7.3. Decreased community structure and population "well being" compared to early studies cannot be attributed entirely to recent acidic condition. Separating potential stress due to lake conditions from that due to heavy biotic predation by sport fishing in a small reservoir is difficult.