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Investigating the Process of Cement Line Maturation on Substrate Surfaces with Submicron UndercutsKo, James Chih-Hsien Jr. 06 January 2011 (has links)
The cement line is the first mineralized matrix deposited on an implant surface during contact osteogenesis forming the bone/implant interface. The hypothesis underlying the present project was that non-collagenous cement line proteins must be deposited into the submicron undercuts on substrate surfaces prior mineralization. In vitro osteogenic cultures were used to grow bone nodules on Thermanox® coverslips modified with calcium phosphate nanocrystals, creating an undercutted surface. Electron microscopy was used to observe cement line formation. BSP immunogold labelling was used to determine if the cement line organic matrix is deposited within undercuts prior mineralization. The results showed the deposited bone nodules, and on test coverslips the deposited cement line was thicker and evenly distributed than control. Furthermore, positive BSP labelling was found within the undercuts prior to cement line mineralization. Thus, it can be concluded that cement line proteins are deposited into submicron undercuts on substrate surfaces prior to mineralization.
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Investigating the Process of Cement Line Maturation on Substrate Surfaces with Submicron UndercutsKo, James Chih-Hsien Jr. 06 January 2011 (has links)
The cement line is the first mineralized matrix deposited on an implant surface during contact osteogenesis forming the bone/implant interface. The hypothesis underlying the present project was that non-collagenous cement line proteins must be deposited into the submicron undercuts on substrate surfaces prior mineralization. In vitro osteogenic cultures were used to grow bone nodules on Thermanox® coverslips modified with calcium phosphate nanocrystals, creating an undercutted surface. Electron microscopy was used to observe cement line formation. BSP immunogold labelling was used to determine if the cement line organic matrix is deposited within undercuts prior mineralization. The results showed the deposited bone nodules, and on test coverslips the deposited cement line was thicker and evenly distributed than control. Furthermore, positive BSP labelling was found within the undercuts prior to cement line mineralization. Thus, it can be concluded that cement line proteins are deposited into submicron undercuts on substrate surfaces prior to mineralization.
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Synergistic effect of sulfonation followed by precipitation of amorphous calcium phosphate on the bone-bonding strength of carbon fiber reinforced polyetheretherketone / アパタイト核処理による炭素繊維強化PEEKへの骨結合力の強化についてTakaoka, Yusuke 24 July 2023 (has links)
京都大学 / 新制・課程博士 / 博士(医学) / 甲第24836号 / 医博第5004号 / 新制||医||1068(附属図書館) / 京都大学大学院医学研究科医学専攻 / (主査)教授 安達, 泰治, 教授 森本, 尚樹, 教授 上杉, 志成 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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Evaluation of bioactivity of alkali- and heat-treated titanium using fluorescent mouse osteoblasts / 蛍光タンパク導入マウス由来骨芽細胞を用いたアルカリ加熱処理チタンの生体活性能の評価Tsukanaka, Masako 24 March 2014 (has links)
京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第18146号 / 医博第3866号 / 新制||医||1002(附属図書館) / 31004 / 京都大学大学院医学研究科医学専攻 / (主査)教授 鈴木 茂彦, 教授 妻木 範行, 教授 戸口田 淳也 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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In vivo study of the early bone-bonding ability of Ti meshes formed with calcium titanate via chemical treatments / 化学処理によりチタン酸カルシウム層を形成したチタンメッシュの早期骨結合能の生体内評価Yi, Tian 23 March 2016 (has links)
Final publication is available at http://link.springer.com/article/10.1007%2Fs10856-015-5612-2 / 京都大学 / 0048 / 新制・課程博士 / 博士(医学) / 甲第19566号 / 医博第4073号 / 新制||医||1013(附属図書館) / 32602 / 京都大学大学院医学研究科医学専攻 / (主査)教授 安達 泰治, 教授 戸口田 淳也, 教授 鈴木 茂彦 / 学位規則第4条第1項該当 / Doctor of Medical Science / Kyoto University / DFAM
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The Comparative Performance of Micro- and Nano-topographically Complex Endosseous Implant Surfaces in Normoglycemic and Hyperglycemic SubjectsBell, Spencer 11 July 2013 (has links)
Endosseous implants have notably high success rates, yet a small percentage of implants still fail for unidentified reasons. Recent literature points to hyperglycemia, resulting from untreated or undiagnosed diabetes, as a possible contraindication in an otherwise apparently healthy population. To investigate the effect of surface design on peri-implant healing in the presence of hyperglycemia, STZ-treated rats were implanted with custom rectangular implants of two surface topographies: grit blasted (GB) and grit-blast with a calcium phosphate nanotopography (GB-DCD). Tensile testing was conducted at 5, 7, and 9 days post-operative. Results demonstrated hyperglycemia to delay early stages of the peri-implant healing. Contact osteogenesis was increased along the GB-DCD surface, even in an environment of uncontrolled hyperglycemia, and the GB-DCD surface outperformed the GB surface in both healthy and hyperglycemic animals, showing peri-implant bone matured more rapidly on nanotopographically complex surfaces, even in the presence of uncontrolled hyperglycemia.
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