Phosphorus is the main limiting nutrient for plant growth in large areas of the world and the availability of phosphorus to plants and microbes can be strongly affected by soil properties. Even though the phosphorus cycle has been studied extensively, much remains unknown about the key processes governing phosphorus availability in different environments. In this thesis the complex dynamics of soil phosphorus and its availability were studied by relating various phosphorus fractions and soil characteristics to microbial respiration kinetics. The soils used represent a range of aluminium, iron, carbon and total phosphorus content, and were located in four different biomes: subtropical forest, warm temperate forest, boreal forest and tundra. The results showed that NaOH extractable phosphorus, a fraction previously considered to be available to plants only over long time scales, can be accessed by microbes in days or weeks. Microbial phosphorus availability was not related to aluminium or iron content in any of the studied systems, not even in highly weathered soils with high aluminium and iron content. This is in contrast with other studies of soils with high sorption capacity and shows the variability of factors that govern phosphorus availability in different environments. In the boreal forest chronosequence, no difference could be seen with age in total phosphorus content or concentrations of occluded phosphorus forms. However, there were lower concentrations of labile phosphorus forms in older systems, which were correlated with a decrease in microbial respiration. This was most likely related to organic matter quality in the system, and not to geochemical factors. Phosphorus availability was linked to differences in topography (water regime) and vegetation in the tundra ecosystems. The results suggest that the availability of phosphorus, both for microbes and plants, was lower on the meadow vegetation sites compared to the two types of heath vegetation. Many factors are important for phosphorus availability in soils, but these results suggest that microbes can access less available phosphorus if not restricted by carbon, and this may be important in regard to forest management practices as well as effects of environmental change.

This research assesses whether a refinement of the temporal resolution of tree-ring data can improve our understanding of the radial growth-climate relationship. Two study sites in Northern Labrador were chosen, one coastal (Nain), and the other inland (Kamestastin). In Nain, microcore samples were taken weekly from the same five white spruce (Picea glauca) trees over the 2014 growing season. After cross sections were made and stained, the resulting 10µm thin radii provided a direct view of active ring development. In coastal Labrador, radial growth was initiated during the last week of June 2014, and ceased by August 25th. Circumference band dendrometers were installed on white spruce trees at both the Nain and Kamestastin sites. The dendrometers were used to measure micrometre-scale changes over the 2014 growing season. Analogous records of temperature were collected with equal temporal resolution, from an Environment Canada climate station (#8502800), and via a programmable data logger (UX120-006M, Onset HOBO). Correlation function analysis determined the relationship between daily temperature variables and daily variations in stem size. A strong relationship was found between minimum daily temperature and daily stem size at both sites over the eight week long growing season. Traditional dendrochronological sampling methods were utilized to retrieve tree cores from white spruce and eastern larch (Larix laricina) in Nain and Kamestastin. Site-specific master growth chronologies were created using crossdating and standardization techniques. After establishing long term records of monthly temperature and accumulated growing degree-days (GDD) at both study sites, a linear regression analysis was undertaken to determine the suitability of these two variables as predictors of annual-radial growth. An accumulated June/July GDD index was identified as an overall better predictor of annual ring-width than mean monthly temperature variables in northern Labrador. Exploring radial growth on an intra-annual scale helped to improve our understanding of the complex radial growth-climate relationship in Labrador. This allows for a strengthening of tree rings as a proxy climate indicator in remote regions of the northern boreal forest. The findings from this thesis provide the tools necessary to improve upon long-term climate reconstruction and forecasts of boreal forest structure in the face of climate change.

Climate change will pose increasing challenges to forest managers working to achieve sustainable forest management in the boreal forest. A logical starting point for climate change adaptation is to proactively identify management practices and policies that have a higher likelihood of achieving management objectives across a wide range of potential climate futures. This research implemented an approach to identifying such measures by tapping into the experiential knowledge base of local forest practitioners. The assessment was organized according to a structured decision-making (SDM) approach. Northern forest practitioners consider the goals of climate change adaptation to be synonymous with those of sustainable forest management indicating that the criteria for the conservation and sustainable management of boreal forests as defined by the Montréal Process are suitable objectives against which the performance of alternative adaptation options can be assessed. The case study area for this research was the Champagne and Aishihik Traditional Territory of southwest Yukon where a climatically-driven, large-scale spruce bark beetle disturbance has been driving forest management planning yet climate change considerations have not been directly addressed in the planning process. Twenty-four adaptation options were identified as being important to implement in forest development areas to achieve regional goals and objectives of forest management across three scenarios of climate change. In addition, the performance of alternative strategies to re-establish forests was assessed. Results indicate that the applicability of alternative forest renewal adaptation strategies is strongly related to the objectives of forest management which differed across the forest management planning area. However, since none of the strategies were judged to perform highly across any of the scenarios of climate change, additional work is needed to explore whether a threshold of acceptability can be met even with the adoption of adjustments to forest management policies and practices. If not, management objectives themselves may need to be revised. An extensive list of research and monitoring needs were also identified, an indication that climate change is providing the imperative for a more comprehensive research and monitoring program to support the sustainable management of forest resources in this region. The next steps in a SDM approach are to implement adaptation options and strategies deemed appropriate and to monitor their performance in achieving management objectives within an adaptive management context.

No description available.

Archaeological sites in the boreal forest are facing threats due to urban development, resource exploitation, vandalism, and infrastructure development, among others. In the context of archaeological site preservation as a public issue, I examine the perspectives of various publics towards the preservation and stewardship of archaeological sites in the boreal forest. Through a series of interviews, I examine the opinions of three publics involved in the archaeological process in Ontario: developers, First Nations, and archaeologists. I outline the participants??? opinions on the meaning and goals of preservation, the preservation of non-physical aspects of sites, such as oral history and site spirituality, preservation methods, site ownership and access, land use and development, involvement in the archaeological process, and funding. I also identify common themes which presented themselves throughout the interview process, such as the importance of education; the necessity for communication, collaboration, and cooperation; the problem of artifact curation; the perceived lack of genuine government involvement; and the publication of cultural resource management (CRM) archaeology???s ???grey literature???. Finally, I present suggestions on the preservation of archaeological sites which take into account the participants??? perspectives uncovered during the interview process. I conclude that preserving archaeological sites can be done using three techniques: education; communication, collaboration, and compromise; and using one of three general methods to preserve sites and artifacts. Education can be used to create public issues, teach people about the importance of archaeology and archaeological sites, and teach the involved publics about the goals and methods of CRM archaeology in Ontario. Encouraging communication, collaboration, and compromise between the interested publics includes the perspectives of formerly neglected parties, builds relationships between publics, and creates newly vested interests in site preservation. Three methods to preserve archaeological sites include site stabilization and monitoring, allowing sites to decay naturally, and excavating sites and curating the artifacts and oral histories for the long-term.

Soils of Northern Hemisphere forests contain a large part of the global terrestrial carbon (C) pool. Even small changes in this pool can have large impact on atmospheric [CO2] and the global climate. Soil respiration is the largest terrestrial C flux to the atmosphere and can be divided into autotrophic (from roots, mycorrhizal hyphae and associated microbes) and heterotrophic (from decomposers of organic material) respiration. It is therefore crucial to establish how the two components will respond to changing environmental factors. In this thesis I studied the effect of elevated atmospheric [CO2] (+340 ppm, 13C-depleted) and elevated air temperature (2.8-3.5 oC) on soil respiration in a whole-tree chamber (WTC) experiment conducted in a boreal Norway spruce forest. In another spruce forest I used multivariate modelling to establish the link between day-to-day variations in soil respiration rates and its δ13C, and above and below ground abiotic conditions. In both forests, variation in δ13C was used as a marker for autotrophic respiration. A trenching experiment was conducted in the latter forest in order to separate the two components of soil respiration. The potential problems associated with the trenching, increased root decomposition and changed soil moisture conditions were handled by empirical modelling. The WTC experiment showed that elevated [CO2] but not temperature resulted in 48 to 62% increased soil respiration rates. The CO2-induced increase was in absolute numbers relatively insensitive to seasonal changes in soil temperature and data on δ13C suggest it mostly resulted from increased autotrophic respiration. From the multivariate modelling we observed a strong link between weather (air temperature and vapour pressure deficit) and the day-to-day variation of soil respiration rate and its δ13C. However, the tightness of the link was dependent on good weather for up to a week before the respiration sampling. Changes in soil respiration rates showed a lag to weather conditions of 2-4 days, which was 1-3 days shorter than for the δ13C signal. We hypothesised to be due to pressure concentration waves moving in the phloem at higher rates than the solute itself (i.e., the δ13C–label). Results from the empirical modelling in the trenching experiment show that autotrophic respiration contributed to about 50% of total soil respiration, had a great day-to-day variation and was correlated to total soil respiration while not to soil temperature or soil moisture. Over the first five months after the trenching, an estimated 45% of respiration from the trenched plots was an artefact of the treatment. Of this, 29% was a water difference effect and 16% resulted from root decomposition. In conclusion, elevated [CO2] caused an increased C flux to the roots but this C was rapidly respired and has probably not caused changes in the C stored in root biomass or in soil organic matter in this N-limited forest. Autotrophic respiration seems to be strongly influenced by the availability of newly produced substrates and rather insensitive to changes in soil temperature. Root trenching artefacts can be compensated for by empirical modelling, an alternative to the sequential root harvesting technique.

Climate change will pose increasing challenges to forest managers working to achieve sustainable forest management in the boreal forest. A logical starting point for climate change adaptation is to proactively identify management practices and policies that have a higher likelihood of achieving management objectives across a wide range of potential climate futures. This research implemented an approach to identifying such measures by tapping into the experiential knowledge base of local forest practitioners. The assessment was organized according to a structured decision-making (SDM) approach. Northern forest practitioners consider the goals of climate change adaptation to be synonymous with those of sustainable forest management indicating that the criteria for the conservation and sustainable management of boreal forests as defined by the Montréal Process are suitable objectives against which the performance of alternative adaptation options can be assessed. The case study area for this research was the Champagne and Aishihik Traditional Territory of southwest Yukon where a climatically-driven, large-scale spruce bark beetle disturbance has been driving forest management planning yet climate change considerations have not been directly addressed in the planning process. Twenty-four adaptation options were identified as being important to implement in forest development areas to achieve regional goals and objectives of forest management across three scenarios of climate change. In addition, the performance of alternative strategies to re-establish forests was assessed. Results indicate that the applicability of alternative forest renewal adaptation strategies is strongly related to the objectives of forest management which differed across the forest management planning area. However, since none of the strategies were judged to perform highly across any of the scenarios of climate change, additional work is needed to explore whether a threshold of acceptability can be met even with the adoption of adjustments to forest management policies and practices. If not, management objectives themselves may need to be revised. An extensive list of research and monitoring needs were also identified, an indication that climate change is providing the imperative for a more comprehensive research and monitoring program to support the sustainable management of forest resources in this region. The next steps in a SDM approach are to implement adaptation options and strategies deemed appropriate and to monitor their performance in achieving management objectives within an adaptive management context.

Abstract Maintaining biodiversity in boreal forest landscapes in conjunction with forestry is a challenging task. This requires ecological understanding that is based on empirical research. In this thesis, I examined spatial and temporal occupancy patterns as well as predictability of the occurrence of the Siberian flying squirrel (Pteromys volans L.) in Finland. I used thematic maps which matched habitat requirements of the flying squirrel in forested landscapes and data on species presence and absence, which were gathered in suitable forest habitats. The results of this thesis provide applications for landscape management. First, the preferred habitat characteristics of the flying squirrel were linked to available forest data. In addition, some predictive habitat models could be used to estimate the distribution of the flying squirrel within a region. Second, based on a five year study the forests were classified as continuously occupied, continuously unoccupied and variable-occupancy patches. The dynamic occupancy pattern emphasizes the need for repeated surveys to also locate the seldom-used suitable habitats in a landscape. Third, a comparison of simulated future scenarios in long-term forest planning suggested that flying squirrel habitat might be maintained without considerable loss of timber in a landscape. Thus, a combination of ecological and economic goals in forestry planning is an encouraging alternative. Fourth, there were more polypore species in forests occupied by the flying squirrel. This suggests that conservation of the flying squirrel habitats would protect other naturally co-occurring species, and thus the flying squirrel could be assigned as an umbrella species in mature spruce-dominated forests. Based on these findings, I suggest that the flying squirrel could be used as one of the target species for forest management in boreal forest landscapes. Further research challenges are related to the examination of habitat thresholds and to the projection of future scenarios where ecological, economic and social aspects are combined to assist in complex decision making processes.

Among dinitrogen (N2)-fixing some cyanobacteria can establish symbiosis with a broad range of host plants from all plant lineages including bryophytes, ferns, gymnosperms, and angiosperms. In the boreal forests, the symbiosis between epiphytic cyanobacteria and feathermosses Hylocomium splendens and Pleurozium schreberi is ecologically important. The main input of biological N to the boreal forests is through these cyanobacteria, and thus, they greatly contribute to the productivity of this ecosystem. Despite the ecological relevance of the feathermoss symbiosis, our knowledge about the establishment and maintenance of cyanobacterial-plant partnerships in general is limited, and particularly our understanding of the feathermoss symbiosis is rudimentary. The first aim of this thesis was to gain insight on the genomic rearrangements that enabled cyanobacteria to form a symbiosis with feathermosses, and their genomic diversity and similarities with other plant-symbiotic cyanobacteria partnerships. Genomic comparison of the feathermoss isolates with the genomes of free-living cyanobacteria highlighted that functions such as chemotaxis and motility, the transport and metabolism of organic sulfur, and the uptake of phosphate and amino acids were enriched in the genome of plant-symbiotic cyanobacteria. The second aim of this PhD study was to identify cyanobacterial molecular pathways involved in forming the feathermoss symbiosis and the regulatory rewiring needed to maintain it. Global transcriptional and post-transcriptional regulation in cyanobacteria during the early phase of establishment of the feathermoss symbiosis, and after colonization of the moss were investigated. The results revealed that the putative symbiotic gene repertoire includes pathways never before associated with cyanobacteria-plant symbioses, such as nitric-oxide sensing and regulation, and the transport and metabolism of aliphatic sulfonate. The third aim was to explore the role of the cyanobacterial community in contributing to the temporal variability of N2-fixation activity. Results from a field-study showed that temporal variation in N2-fixation rates could be explained to a high degree by changes in cyanobacterial community composition and activity. In particular, the cyanobacteria belonging to the genus Stigonema - although not dominating the community- appeared to be the main contributors to the N2-fixation activities. Based on this result, it is suggested that this genus is responsible for the main input of N in the boreal forest ecosystems. The last aim was to understand how the relationship between cyanobacterial community composition and N2-fixation activity will be affected by climatic changes such as, increased temperature (11oC compared to 19oC) and CO2 level (500 ppm compared to 1000 ppm). Laboratory experiments highlighted that 30 weeks of combined elevation of temperature and CO2 resulted in increased N2-fixation activity and moss growth rates. The observed increases were suggested to be allocated to reduced cyanobacterial diversity and changes in community composition, resulting in the dominance of cyanobacteria adapted to the future abiotic condition. / <p>At the time of the doctoral defense, the following papers were unpublished and had a status as follows: Paper 1: Manuscript. Paper 4: Manuscript.</p>

Climate change will pose increasing challenges to forest managers working to achieve sustainable forest management in the boreal forest. A logical starting point for climate change adaptation is to proactively identify management practices and policies that have a higher likelihood of achieving management objectives across a wide range of potential climate futures. This research implemented an approach to identifying such measures by tapping into the experiential knowledge base of local forest practitioners. The assessment was organized according to a structured decision-making (SDM) approach. Northern forest practitioners consider the goals of climate change adaptation to be synonymous with those of sustainable forest management indicating that the criteria for the conservation and sustainable management of boreal forests as defined by the Montréal Process are suitable objectives against which the performance of alternative adaptation options can be assessed. The case study area for this research was the Champagne and Aishihik Traditional Territory of southwest Yukon where a climatically-driven, large-scale spruce bark beetle disturbance has been driving forest management planning yet climate change considerations have not been directly addressed in the planning process. Twenty-four adaptation options were identified as being important to implement in forest development areas to achieve regional goals and objectives of forest management across three scenarios of climate change. In addition, the performance of alternative strategies to re-establish forests was assessed. Results indicate that the applicability of alternative forest renewal adaptation strategies is strongly related to the objectives of forest management which differed across the forest management planning area. However, since none of the strategies were judged to perform highly across any of the scenarios of climate change, additional work is needed to explore whether a threshold of acceptability can be met even with the adoption of adjustments to forest management policies and practices. If not, management objectives themselves may need to be revised. An extensive list of research and monitoring needs were also identified, an indication that climate change is providing the imperative for a more comprehensive research and monitoring program to support the sustainable management of forest resources in this region. The next steps in a SDM approach are to implement adaptation options and strategies deemed appropriate and to monitor their performance in achieving management objectives within an adaptive management context. / Forestry, Faculty of / Graduate