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  • About
  • The Global ETD Search service is a free service for researchers to find electronic theses and dissertations. This service is provided by the Networked Digital Library of Theses and Dissertations.
    Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
1

Bovine leukosis virus and cell kinetics

Takashima, Ikuo. January 1900 (has links)
Thesis--University of Wisconsin--Madison. / Typescript. Vita. Description based on print version record. Includes bibliographical references.
2

Bovine leukemia virus transmission studies

Baumgartener, Lois E. January 1981 (has links)
Thesis (Ph. D.)--University of Wisconsin--Madison, 1981. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references.
3

Bovine leukemia : etiologic, pathogenetic and diagnostic studies

Muhammed, G. Sani A January 2010 (has links)
Typescript, etc. / Digitized by Kansas Correctional Industries
4

Attempted transmission of bovine lympho-sarcoma to ex-axenic mice

Grover, Wayne Merle. January 1966 (has links)
Call number: LD2668 .T4 1966 G883 / Master of Science
5

Decreasing perinatal bovine leukosis virus infection in calves

Nagy, Dusty W., January 2006 (has links)
Thesis (Ph. D.)--University of Missouri-Columbia, 2006. / Title from title screen of research.pdf file (viewed on December 22, 2006) The entire dissertation/thesis text is included in the research.pdf file; the official abstract appears in the short.pdf file (which also appears in the research.pdf); a non-technical general description, or public abstract, appears in the public.pdf file. Vita. "May 2006" Includes bibliographical references.
6

Etude de la régulation transcriptionnelle du virus de la leucémie bovine: rôle de la chromatine et des facteurs de transcription PU.1 et Sp1/Sp3

Dekoninck, Ann January 2005 (has links)
Doctorat en Sciences / info:eu-repo/semantics/nonPublished
7

Avaliação do teste de imunoperoxidase para detecção de anticorpos contra o vírus da leucose bovina (BLV) / Evaluation of immunoperoxidase test for the detection of antibodies against the bovine leukosis virus (BLV)

Castro, Clarissa Caetano de 31 March 2011 (has links)
Made available in DSpace on 2014-08-20T14:38:03Z (GMT). No. of bitstreams: 1 dissertacao_clarissa_castro.pdf: 569054 bytes, checksum: b9876922c1664dec56ef9c9d4ff5cb3a (MD5) Previous issue date: 2011-03-31 / The enzootic bovine leukosis is an illness caused by the bovine leukosis virus (BLV) which provokes a persistent infection in cattle and is responsible for significant economical losses to the bovines, mainly dairy cattle. It is widely spread in Brazilian cattle and it can come up in three forms: asymptomatic infection, persistent lymphocytosis and lymphosarcoma. The animals that have antibodies against the BLV must be eliminated or separated from the rest of the cattle, because they are carriers and disseminators of the virus during all their lifetimes. The diagnosis of this disease is essential for control and eradication strategies based on the segregation of infected animals in order to avoid or attenuate the transmission of the virus, and consequently, minimize the economical losses caused by the disease. During the BLV infection antibodies are produced against the main viral proteins, gp 51, gp 30 (envelope glicoproteins) and p24 (capsid protein). The agar gel immunodiffusion (AGID) and the enzyme-linked immunosorbent assay (ELISA) tests are the most used for diagnosis. In this report the immunoperoxidase technique (peroxidase linked assay - PLA) was evaluated in order to detect antibodies against the BLV. The results obtained in the PLA were compared with the AGID test and the specificity of the positive ones was confirmed by the Western blotting (WB) technique. Two hundred and one bovine serum samples of cattle coming from the city of Pelotas were tested: 59% (119) were positive in PLA and 26% (53) were positive in AGID. All the AGID positive samples were also PLA positive. From the 32,8% (66) of the conflicting serum results just eight were confirmed as positive in WB, indicating that the rest of the results were false-positive and showing that AGID failed in identifying 4% of the BLV positive animals. The PLA technique for the diagnosis of the BLV infection demonstrated to be very useful to use in control programs because it was more sensitive when compared to the AGID technique. However, the occurrence of false-positive results by the PLA makes its use unviable in eradication programs that involve the sacrifice of seropositive animals. / A leucose enzoótica dos bovinos (EBL) é uma enfermidade causada pelo vírus da leucose bovina (BLV), que ocasiona uma infecção persistente em bovinos, e é responsável por perdas econômicas significativas à pecuária, principalmente a leiteira. Amplamente disseminada no rebanho brasileiro, pode ser manifestada de três formas: aleucêmica, linfocitose persistente e linfossarcoma. Os animais com anticorpos contra o BLV deverão ser eliminados ou separados do restante do rebanho, pois significa que são portadores e disseminadores do vírus por toda a vida. O diagnóstico desta doença é essencial para estratégias de controle e erradicação baseadas na segregação de animais infectados no intuito de evitar ou amenizar a transmissão do vírus e, consequentemente, minimizar as perdas econômicas causadas pela doença. Durante a infecção pelo BLV são produzidos anticorpos contra as principais proteínas virais, gp51, gp30 (glicoproteínas do envelope) e p24 (proteína do capsídeo). O teste de imunodifusão em gel de ágar (agar gel immunodiffusion AGID) e o ensaio imunoenzimático (enzyme-linked immunosorbent assay ELISA) são os mais utilizados para diagnóstico. No presente trabalho foi avaliada a técnica de imunoperoxidase (peroxidase linked assay PLA) na detecção de anticorpos contra o BLV. Os resultados obtidos na PLA foram comparados com o teste de AGID e a especificidade dos positivos confirmada pela técnica de Western blotting (WB). Foram testadas 201 amostras de soro de bovinos provenientes de propriedades localizadas no município de Pelotas: 59% (119) foram positivos por PLA e 26% (53) positivos por AGID. Todas as amostras positivas na AGID foram também positivas na PLA. Dos 32,8% (66) dos soros com resultados conflitantes apenas oito foram confirmados como positivos no WB, indicando serem falso-positivos os demais resultados e constatando que a AGID falhou em identificar 4% de animais positivos para EBL. A técnica de PLA para diagnóstico de infecção pelo BLV se mostrou muito útil para uso em programas de controle devido à maior sensibilidade da técnica quando comparada com o teste de AGID. Contudo, a ocorrência de resultados falso-positivos pela PLA inviabiliza o seu uso em programas de erradicação que envolva o sacrifício do animal soropositivo.
8

Correlação entre a atipia linfocitária e o perfil imunológico de animais infectados pelo vírus da leucose enzoótica bovina / Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows

Spinola, Tatiana de Rezende 09 September 2010 (has links)
Dentre as enfermidades que causam alterações hematológicas em bovinos podemos citar a leucose enzoótica bovina (LEB). Podendo em alguns animais, determinar formações tumorais por infiltração de células mononucleares (linfócitos, pró-linfócitos e linfócitos atípicos), em diferentes tecidos. A doença possui quadro sintomático pleomórfico e alterações hematológicas, evidenciadas por leucocitose e linfocitose persistente, com aumento de formas linfocitárias atípicas. Desta forma o presente estudo tem como objetivo avaliar a atipia linfocitária em bovinos da raça Holandesa Preto e Branco em animais soropositivos com ou sem a presença de linfocitose persistente e animais soronegativos para LEB e correlacioná-la com a apoptose de células CD5+ e a proliferação linfocitária. Assim, foram selecionados 56 animais, divididos de acordo com o leucograma e o sorodiagnóstico da LEB pela imunodifusão em ágar gel e pelo ensaio imunoenzimático em: negativos (n = 25), alinfocitóticos (AL, n= 12) e com linfocitose persistente (LP, n=19). Destes, 15 animais tiveram suas amostras sanguíneas avaliadas quanto a proliferação de linfócitos e apoptose. Os resultados deste estudo mostraram que, a contagem de leucócitos totais e os valores absolutos e relativos de linfócitos atípicos foram maiores no grupo LP. Em relação aos linfócitos atípicos, os linfócitos sombra de Gümprecht, linfócito monocitóide e linfócito com núcleo duplo, foram encontrados nos três grupos de estudo, porém mais comumente em animais infectados pelo VLEB, predominantemente no grupo manifestando linfocitose persistente. Este trabalho demonstrou uma menor proliferação de linfócitos nos animais infectados pelo VLEB manifestando LP, associado a uma menor apoptose de células CD5+. Na análise da correlação entre a apoptose de células CD5+ e a porcentagem das populações linfocitárias entre os 15 animais, observou-se que a apoptose tem uma correlação positiva com os linfócitos com núcleo duplo, o que não ocorreu nas demais populações celulares. Na correlação entre a proliferação, foi observado nos 15 animais, que quanto maior a porcentagem de linfócitos menor é a proliferação e quanto maior a porcentagem de linfócitos monocitóides menor a proliferação, o que não ocorreu nas demais populações celulares. Portanto, a manifestação da LP em animais naturalmente infectados pelo VLEB, pode ser associada ao aumento de linfócitos atípicos do tipo sombra de Gümprecht, linfócito monocitóide e linfócito núcleo duplo e da menor apoptose das células CD5 + e da menor proliferação de linfócitos / Among the diseases that cause hematological changes in cattle can cite the enzootic bovine leukemia (EBL). And in some animals, to determine tumor formations by infiltration of mononuclear cells (lymphocytes, pro-lymphocytes and atypical lymphocytes) in different tissues. The disease has symptoms very pleomorphic and hematological changes, evidenced by leukocytosis, and persistent lymphocytosis, an increase of atypical lymphocyte forms. Thus this study aims to evaluate the atypical lymphocyte in Holstein Black and White in seropositive animals with or without the presence of persistent lymphocytosis and animals seronegative LEB and correlate them with apoptosis of cells CD5 + and lymphocyte proliferation. Thus, we selected 56 animals, divided according to the WBC and serodiagnosis of LEB by agar gel immunodiffusion and by enzyme immunoassay in: negative (n = 25), non- lymphocytic (AL, n = 12) and with persistent lymphocytosis (PL, n = 19). Of these, 15 animals had their blood samples evaluated for lymphocyte proliferation and apoptosis. The results of this study showed that in total leukocytes and absolute and relative values of atypical lymphocytes were higher in group LP. Regarding atypical lymphocytes, lymphocytes Gumprecht shadow, lymphocyte and monocytoid lymphocytes with dual core, were found in the three study groups, but more commonly in animals infected VLEB, predominantly in the group with persistent lymphocytosis. This study demonstrated a reduced proliferation of lymphocytes in animals infected VLEB expressing LP, associated with lower apoptosis of CD5 + cells. In analyzing the correlation between apoptosis of cells and the percentage of CD5 + lymphocyte populations among the 15 animals, we observed that apoptosis has a positive correlation with lymphocytes with dual core, which did not occur in other cell populations. Correlation between proliferation, was observed in 15 animals, the higher the percentage of lymphocyte proliferation and lower the higher the percentage of monocytoid lymphocytes less proliferation, which did not occur in other cell populations. Therefore, the expression of LP in animals naturally infected VLEB may be associated with increased atypical lymphocytes of the type of shadow Gumprecht, lymphocyte and lymphocyte monocytoid dual-core and reduced apoptosis of CD5 + cells and reduced lymphocyte proliferation
9

Correlação entre a atipia linfocitária e o perfil imunológico de animais infectados pelo vírus da leucose enzoótica bovina / Correlation between immunological profile and atypical lymphocytes in bovine leukemia virus infected dairy cows

Tatiana de Rezende Spinola 09 September 2010 (has links)
Dentre as enfermidades que causam alterações hematológicas em bovinos podemos citar a leucose enzoótica bovina (LEB). Podendo em alguns animais, determinar formações tumorais por infiltração de células mononucleares (linfócitos, pró-linfócitos e linfócitos atípicos), em diferentes tecidos. A doença possui quadro sintomático pleomórfico e alterações hematológicas, evidenciadas por leucocitose e linfocitose persistente, com aumento de formas linfocitárias atípicas. Desta forma o presente estudo tem como objetivo avaliar a atipia linfocitária em bovinos da raça Holandesa Preto e Branco em animais soropositivos com ou sem a presença de linfocitose persistente e animais soronegativos para LEB e correlacioná-la com a apoptose de células CD5+ e a proliferação linfocitária. Assim, foram selecionados 56 animais, divididos de acordo com o leucograma e o sorodiagnóstico da LEB pela imunodifusão em ágar gel e pelo ensaio imunoenzimático em: negativos (n = 25), alinfocitóticos (AL, n= 12) e com linfocitose persistente (LP, n=19). Destes, 15 animais tiveram suas amostras sanguíneas avaliadas quanto a proliferação de linfócitos e apoptose. Os resultados deste estudo mostraram que, a contagem de leucócitos totais e os valores absolutos e relativos de linfócitos atípicos foram maiores no grupo LP. Em relação aos linfócitos atípicos, os linfócitos sombra de Gümprecht, linfócito monocitóide e linfócito com núcleo duplo, foram encontrados nos três grupos de estudo, porém mais comumente em animais infectados pelo VLEB, predominantemente no grupo manifestando linfocitose persistente. Este trabalho demonstrou uma menor proliferação de linfócitos nos animais infectados pelo VLEB manifestando LP, associado a uma menor apoptose de células CD5+. Na análise da correlação entre a apoptose de células CD5+ e a porcentagem das populações linfocitárias entre os 15 animais, observou-se que a apoptose tem uma correlação positiva com os linfócitos com núcleo duplo, o que não ocorreu nas demais populações celulares. Na correlação entre a proliferação, foi observado nos 15 animais, que quanto maior a porcentagem de linfócitos menor é a proliferação e quanto maior a porcentagem de linfócitos monocitóides menor a proliferação, o que não ocorreu nas demais populações celulares. Portanto, a manifestação da LP em animais naturalmente infectados pelo VLEB, pode ser associada ao aumento de linfócitos atípicos do tipo sombra de Gümprecht, linfócito monocitóide e linfócito núcleo duplo e da menor apoptose das células CD5 + e da menor proliferação de linfócitos / Among the diseases that cause hematological changes in cattle can cite the enzootic bovine leukemia (EBL). And in some animals, to determine tumor formations by infiltration of mononuclear cells (lymphocytes, pro-lymphocytes and atypical lymphocytes) in different tissues. The disease has symptoms very pleomorphic and hematological changes, evidenced by leukocytosis, and persistent lymphocytosis, an increase of atypical lymphocyte forms. Thus this study aims to evaluate the atypical lymphocyte in Holstein Black and White in seropositive animals with or without the presence of persistent lymphocytosis and animals seronegative LEB and correlate them with apoptosis of cells CD5 + and lymphocyte proliferation. Thus, we selected 56 animals, divided according to the WBC and serodiagnosis of LEB by agar gel immunodiffusion and by enzyme immunoassay in: negative (n = 25), non- lymphocytic (AL, n = 12) and with persistent lymphocytosis (PL, n = 19). Of these, 15 animals had their blood samples evaluated for lymphocyte proliferation and apoptosis. The results of this study showed that in total leukocytes and absolute and relative values of atypical lymphocytes were higher in group LP. Regarding atypical lymphocytes, lymphocytes Gumprecht shadow, lymphocyte and monocytoid lymphocytes with dual core, were found in the three study groups, but more commonly in animals infected VLEB, predominantly in the group with persistent lymphocytosis. This study demonstrated a reduced proliferation of lymphocytes in animals infected VLEB expressing LP, associated with lower apoptosis of CD5 + cells. In analyzing the correlation between apoptosis of cells and the percentage of CD5 + lymphocyte populations among the 15 animals, we observed that apoptosis has a positive correlation with lymphocytes with dual core, which did not occur in other cell populations. Correlation between proliferation, was observed in 15 animals, the higher the percentage of lymphocyte proliferation and lower the higher the percentage of monocytoid lymphocytes less proliferation, which did not occur in other cell populations. Therefore, the expression of LP in animals naturally infected VLEB may be associated with increased atypical lymphocytes of the type of shadow Gumprecht, lymphocyte and lymphocyte monocytoid dual-core and reduced apoptosis of CD5 + cells and reduced lymphocyte proliferation
10

Epizootiološki modeli kontrole i mogućnosti primene molekularne dijagnostike u cilju unapređenja aktivnog nadzora enzootske leukoze goveda / Epizootic control models and possibilities ofapplication of molecular diagnostics to improvethe active surveillance of enzootic bovineleukosis

Stanojević Slobodan 22 September 2016 (has links)
<p>Enzootska leukoza goveda je maligna virusna neoplazma retikulohistiocitarnog sistema&nbsp;progresivnog karaktera, koja se karakteri&scaron;e intenzivnim razmnožavanjem limfocita. Ova&nbsp;bolest govedarstvu nanosi velike materijalne &scaron;tete dovodeći do visokog nivoa mortaliteta,&nbsp;pojave učestalijih oportunističkih infekcija, smanjenja produktivnosti i reprodukcije na&nbsp;leukoznim farmama. Imajući u vidu, da govedarstvo predstavlja strate&scaron;ku granu u stočarstvu&nbsp;Srbije i učestvuje u stvaranju znatnog dela nacionalnog dohotka od 1999. godine,&nbsp;preduzimana su sistematska dijagnostička ispitivanja ra&scaron;irenosti ELG na farmama goveda&nbsp;kako u intenzivnoj tako i od 2000. godine i u ekstenzivnoj proizvodnji goveda. Kori&scaron;ćene su&nbsp;savremene metode dijagnostike ELG najpre agargel-imunodifuzioni test (AGID), a potom i&nbsp;indirektna imunoenzimska metoda (ELISA) kao dijagnostički test izbora. Na osnovu&nbsp;dobijenih epizootiolo&scaron;kih podataka sprovo&ntilde;ene su mere za suzbijanje i iskorenjivanje EGL na<br />farmama goveda. U zavisnosti od tehnolo&scaron;kog procesa proizvodnje, kao i od ekonomskih&nbsp;mogućnosti farmi sprovo&ntilde;eni su različiti modeli kontrole i eradikacije.<br />ELG predstavlja veoma ozbiljan zdravstveni i ekonomski problem za govedarsku<br />proizvodnju posebno za zapate mlečnih goveda, a po&scaron;to se i pored sprovo&ntilde;enja mera za&nbsp;eradikaciju, leukoza jo&scaron; uvek zadržala u nekim na&scaron;im stadima i zapatima goveda smatrali smo&nbsp;da bi trebalo preispitati dosada&scaron;nju strategiju kontrole ELG.<br />Razmatrane su pote&scaron;koće u sprovo&ntilde;enju mera za suzbijanje i iskorenjivanje leukoze goveda&nbsp;kao i potreba za izučavanjem epizootiologije, odnosno kontrole kretanja leukoze u&nbsp;trakozvanim leukoznim zapatima koji predstavljaju potencijalne izvore &scaron;irenja leukoze.&nbsp;Imajući u vidu navedeno, cilj ovog ispitivanja je bio ustanoviti stepen ra&scaron;irenosti ELG i&nbsp;ispitaju do sada kori&scaron;ćeni programi kontrole i eradikacije oboljenja. Zadaci ovog istraživanja&nbsp;su bili da se ispitaju pogodnosti pojedinih dijagnostičkih metoda u cilju postavljanja rane&nbsp;dijagnoze oboljenja, istražiti incidenciju i prevalenciju bolesti, proceniti značaj pojedinih&nbsp;puteva preno&scaron;enja u okviru farmi i u regionu, izvr&scaron;iti analizu dosada&scaron;njih rezultata borbe i&nbsp;evaluaciju pojedinih modela, predložiti najpogodnije modele kontrole bolesti u cilju potpune&nbsp;eradikacije bolesti, a tako&ntilde;e razviti i adekvatan model nadzora bolesti kako bi se sprečila&nbsp;njena ponovna pojava i &scaron;irenje. Rezultati serolo&scaron;kih ispitivanja pokazuju da je otkriveno 10.181 pozitivnih životinja, odnosno 8,1% posto životinja u zapatima goveda na velikim farmama. Epizootiolo&scaron;ka situacija na farmama goveda individualnog sektora zahtevala je posebnu pažnju i tu je ispitano 281.369 uzoraka krvi goveda i otkriveno je 567 pozitivnih grla ili 0,4% posto na području 12 op&scaron;tina. Rezultati seroprevalencije predstavljali su ključnu informaciju od značaja za izbor modela kotrole ELG. Odnosno istraživanje efikasnosti pojedinih modela u postizanju rezultata i&nbsp;zadatih ciljeva. Značajnih za izradu komparativne analize kori&scaron;ćenih modela, njihove primene i evaluacije. Epizotiolo&scaron;ki modeli su analizirani, izvr&scaron;ena je njihova evaluacija i preporuka za primenu u epizootiolo&scaron;koj praksi.&nbsp;</p> / <p>Enzootic bovine leucosis is viral malignant neoplasm reticulohistiocytic system and has&nbsp;progressive character, which is characterized by intensive multiplication of lymphocytes.&nbsp;This disease is causing a great material damage to cattle farming, leading to high level of&nbsp;mortality, with the emergence of more frequent infections and a reduction in productivity&nbsp;and reproduction in farms in which the leukosis appeared. Having in mind that the cattle&nbsp;farming is a strategic branch of the Serbian livestock and participates in the creation of a&nbsp;significant part of the national income since 1999, systematic diagnostic tests of the&nbsp;prevalence of EBL were undertaken on cattle farms, both in the intensive cattle production&nbsp;and from 2000 extensive production. Modern diagnostics methods of EBL were used,&nbsp;firstly the agargel-immunodiffusion test (AGID), followed by an indirect enzyme&nbsp;immunoassay method (ELISA) as well as a diagnostic test of choice. Based on the&nbsp;obtained epizootic data, the measures for the control and eradication of EBL were carried&nbsp;out on cattle farms. Depending on the technological process of production, as well as the&nbsp;economic farm opportunities, different models of control and eradication were&nbsp;implemented.<br />EBL is a very serious health and economic problem for cattle farming, especially for<br />dairy cattle herds, and despite the implementation of the measures for the eradication of&nbsp;the disease, leucosis is still held in some of our flocks and herds of cattle. Because of that&nbsp;we thought that the current control strategy EBL should be examined.<br />The difficulties in implementing measures to suppress and eradicate bovine leukosis were&nbsp;discuses and the requirement to study the epizootiology , as well as to control the&nbsp;movement of leukosis in so-called leukostic herds, that represent potential source of&nbsp;spreading the leukosis.<br />Taking into account, the purpose of this study was to establish the degree of<br />prevalence of EBL to examine the programs that were used so far to control and eradicate&nbsp;the diseases. The task of this study was to examine the benefits of specific diagnostic methods in order to establish early diagnosis of the diseases, to see into the incidence and prevalence of the leukosis, to estimate the significance of some routes of transmission within the farm and in the region, and to carry out an analysis of recent results in fighting and evaluation of individual models, to suggest the most appropriate models of disease control in order to complete the eradication of leukosis, but also to develop an adequate model of disease control to prevent its re-emergence and spread. The results of serological tests indicate that 10.181 the positive animals were detected, or 8.1 % percent of the animals in cattle herds in large farms. Epizootiological situation in the cattle farms of individual sectors required special attention and 281.369 blood samples of cattle were examined and the 567 positive cases were discovered, or 0.4 % percent in&nbsp;12 communities. Results of seroprevalence were a key information for the choice of models to control ELG, or to investigate the efficiency of some models to achieve results and goals, and important for the production of comparative analysis of models that were used, their implementation and evaluation. Epizootic models were analyzed, their evaluation was made and recommendations for their implementation in the epizootic practice.&nbsp;</p>

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