The submitted work describes design of the electronic working condition monitoring system for laboratory Is analyzed in detail the issue of categorization of laboratory laminar flow hood, determine characteristic of operating variables and their monitoring. Next is described a functional specification of the whole device and it follows the design concept of the possible solutions along with a selection of components for implementation. At the last part is described proposal communication protocol for communication between a master unit and auxiliary slave unit.

This work deals with the design of the electronic driver system for working conditions monitoring in the laboratory box. The state of the art of the laboratory boxes with the laminar convection is presented. The functional specification is made based on final customer needs, then leads to design of the possible solution. The solution is divided into two independent blocks - Main driver unit and Add-on module. The Main driver unit solution is made upon commercially available solution. The Add-on module is then designed including selection of suitable components, PCB prototype and appropriate firmware. Finally the work presents specification of implemented communication protocol for joining both parts Main driver unit and Add-on module.

This diploma thesis deals with the model design for gearbox. The work is focused to its basic creation and optimization in programs of MBS ADAMS and ANSYS. It includes also short summary of contemporary solutions, which are used, and basic characterization of acoustic quantities.

The diploma thesis deals with the assessment design of two-girder crane bridge for the lifting capacity 50 tons. Span of the crane is 20 meters, wheelbase is 3.15 meters and lifting height is 7 meters. This diploma thesis deals with the numerical solution box beam and using the finite element method is solved buckling. The diploma thesis has been elaborated using the relevant technical literature and standards that contain procedures and recommendations for calculation and designing of bridge cranes.

The aim of this diploma thesis is unified design of box gripper telescopes for Navette and SMC storage machines. The work contains technical report and drawings documentation. The first part of technical report is aim to research of similar constructions used in the world. Followed by own construction solution, including needed calculation. At the final part of work was created a tolerance analysis of new telescope. The drawings documentation contains assembly and subassembly drawings.

Digitaliseringen är ett aktuellt ämne i dagens samhälle, och den snabba utvecklingen gör att redovisningsbranschen utmanas i allt större utsträckning än tidigare. Till följd av detta har det uppstått omfattande förändringar i redovisningskonsulternas dagliga arbete vilket påverkar redovisningsbranschen i stort. Syftet med studien var därmed att undersöka och analysera om en ökad digitalisering av redovisningsarbetet försämrar kvalitén av den produkt som redovisningskonsulter skapar. En empirisk undersökning har genomförts med hjälp av kvalitativ metod, där intervjuer med erfarna redovisningskonsulter skapat grunden för arbetet. För att ytterligare stärka det empiriska underlaget samlades sekundärdata in från de aktuella redovisningsbyråernas hemsidor. En jämförelse med den tidigare forskningen inom området gjordes sedan, där studiens resultat analyserades och tolkningar gavs. Resultaten ansågs inte ge tillräckligt starka indikationer för att digitaliseringen påverkar kvalitén av den produkt som redovisningskonsulter skapar. Därav är slutsatsen för denna studie att redovisningsproduktens kvalité inte tenderar att försämras av de digitaliserade processerna inom redovisningsyrket. / The digitization is a current topic in today's society, and the rapid progress creates greater challenges than before for the accounting industry. Due to this, significant changes have occurred in the accountants daily tasks which affects the industry in general. Thus, the purpose of the study was to investigate and analyze whether an increased digitization of the accounting profession deteriorates the quality of the product that accountants create. An empirical research has also been conducted using qualitative methods, where interviews with senior accountants create the basis for the study. To further strengthen the empirical data, secondary data was collected from the accounting bureaus websites. A comparison was then made with the previous research on the subject, whereupon the results of the study were analyzed and interpreted. The results of the study were not considered strong enough to provide indications that digitization affects the quality of the products that accountants create. Therefore, the conclusion of the study is that the product of the accounting does not seem to be impaired by the digitization within the accounting profession.

In eukaryotes, the spliceosome is a macromolecular ribonucleoprotein machine that excises introns from pre-mRNAs through two sequential transesterification reactions. The chemistry and fidelity of pre-mRNA splicing are dependent upon a series of spliceosomal rearrangements, which are mediated by trans-acting splicing factors. One key class of these factors is the DEAH-box ATPase subfamily of proteins, whose members couple ATP hydrolysis to promote RNP structural rearrangements within the spliceosome. This is typified by Prp22, which promotes release of the spliced mRNA from the spliceosome and ensures fidelity of the second step of splicing. This role is well documented through classical biochemical and yeast genetics methods. Yet very little is known regarding the comings and goings of Prp22 relative to the spliceosome. My thesis research investigated the dynamics of Prp22 during splicing by using single-molecule fluorescence methods that allowed direct observation of these events. To do this, I helped construct a toolkit that combined yeast genetics, chemical biology and Colocalization Single Molecule Spectroscopy (CoSMoS) with in vitro splicing assays. Specifically, my thesis research consisted of CoSMoS splicing experiments in which fluorescently labeled pre-mRNA, spliceosome components and Prp22 were directly visualized and analyzed. Using these methods, I found that Prp22’s interactions with the spliceosome are highly dynamic and reversible. By simultaneously monitoring Prp22 and individual spliceosome subcomplexes, I was able to frame these Prp22 binding events in context relative to specific steps in spliceosome assembly and splicing. These experiments provide insight into how Prp22 promotes mRNA release from the spliceosome and maintains splicing fidelity.

The thesis investigates how one should determine the appropriate transparency of an information processing system from a receiver perspective. Research in the past has suggested that the model should be maximally transparent for what is labeled as ”high stake decisions”. Instead of motivating the choice of a model’s transparency on the non-rigorous criterion that the model contributes to a high stake decision, this thesis explores an alternative method. The suggested method involves that one should let the transparency depend on how well an explanation of the model’s output satisfies the purpose of an explanation. As a result, we do not have to bother if it is a high stake decision, we should instead make sure the model is sufficiently transparent to provide an explanation that satisfies the expressed purpose of an explanation.

[pt] Observando a dificuldade de batimento (match) dos padrões de comportamento das funções de autocorrelação e de autocorrelação parcial teóricas com as respectivas funções e as autocorrelação e de autocorrelação parcial estimadas de uma séries temporal, aliada ao fato da dificuldade em definir um número em específico como delimitador inequívoco do que seja um lag significativo, tornam clara a dose de julgamento subjetivo a ser realizado por um especialista de análise de séries temporais na tomada de decisão sobre a estrutura de Box & Jenkins adequada a ser escolhida para modelar o processo estocástico sendo estudado. A matemática nebulosa permite a criação de sistemas de inferências nebulosas (inferência dedutiva) e representa o conhecimento de forma explícita, através de regras nebulosas, possibilitando, facilmente, o entendimento do sistema em estudo. Por outro lado, um modelo de redes neurais representa o conhecimento de forma implícita, adquirido através de exemplos (dados), possuindo excelente capacidade de generalização (inferência indutiva). Esta tese apresenta um sistema especialista composto de cinco redes neurais nebulosas do tipo retropropagação para o auxílio na análise de séries temporais não sazonais. O sistema indica ao usuário a estrutura mais adequada, dentre as estruturas AR(1), MA (1), AR(2), MA(2) e ARMA(1,1), tomando como base a menor distância Euclidiana entre os valores esperados e as saídas das redes neurais nebulosas. / [en] It is well known the difficulties associated with the tradicional procedure for model identification of the Box & Jenkins model through the pattern matching of the theoretical and estimated ACF and PACF. The decision on the acceptance of the null hypothesis of zero ACF (or PACF) for a given lag is based on a strong asymptotic result, particularly for the PACF, leading, sometimes, to wrong decisions on the identified order of the models. The fuzzy logic allows one to infer system governed by incomplete or fuzzy knowledge (deductive inference) using a staighforward formulation of the problem via fuzzy mathematics. On the other hand, the neural network represent the knowledge in a implicit manner and has a great generalization capacity (inductive inference). In this thesis we built a specialist system composed of 5 fuzzy neural networks to help on the automatic identificationof the following Box & Jenkins ARMA structure AR(1), MA(1), AR(2), MA(2) and ARMA (1,1), through the Euclidian distance between the estimated output of the net and the corresponding patterns of each one of the five structures.

<p>There are several techniques and methods on how to capture data during a Live Box response in computer forensics, but the key towards these acquisitions is to keep the collected data admissible in a judicial court process. Different approaches during a Live Box examination will lead to data changes in the computer, due to the volatile nature of data stored in memory. The inevitable changes of volatile data are what cause the controversy when admitting digital evidence to court room proceedings.</p> <p>The main goal of this dissertation was to create a process model, titled Live Box Computer Preservation Response(LBCPR), that would assist in ensuing validity, reliably and accuracy of evidence in a court of law. This approach maximizes the admissibly of digital data derived from a Live Box response. </p> <p>The LBCPR was created to meet legal and technical requirements in acquiring data from a live computer. With captured Live Box computer data, investigators can further add value to their investigation when processing and analyzing the captured data set, that would have otherwise been permanently unrecoverable upon powering down the machine. By collecting the volatile data prior to conducting Dead Box forensics, there is an increased amount of information that that can be a utilized to understand the state of the machine upon collection when combined with the stored data contents. </p> <p>This study created a comparative analysis on data collection with the LBCPR method versus traditional Dead Box forensics techniques, further proving the expected results of Live Box techniques capturing volatile data. However, due to the structure of the LBCPR, there were enhanced capabilities of obtaining value from the randomization of memory dumps, because of the assistance of the collected logs in the process model. In addition, with the legal admissibility focus, there was incorporation of techniques to keep data admissible in a court of law. </p>