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What kinds of engagement might be possible for young people through the creation and experience of a cyberdrama? How do you create a cyberdrama? These were the questions that underpinned the process for creating the cyberdrama www.cleo-missing.com – a drama that was created to be experienced through a fully mediated form on the Internet. The background for this project involved: exploring the context for creating a web-based cyberdrama with young people; defining cyberdrama, the nature of the work and possible processes and forms; and examining the notion of engagement, looking for possible links between aspects of the aesthetic and the immersive. The process of creating the drama utilised aspects of process drama, a form emerging from the field of drama education as one that offers up huge potential for the creation of on-line interactive drama. The project research suggests that the context for experiencing the work through the Internet means that the experience of “diversion” needs to be considered and is much more likely for many users than that of “immersion”. This is particularly so in view of the ways many young people use the Internet, with common interactions taking on aspects of Bakhtin’s “carnival” (a subversive or alternative order). The experience for participants in creating the drama was characterised by a number of features, but engagement seemed particularly strong when aspects of control were involved or possible. The framing of this experience through the use of various recording technologies was of key significance to this experience of engagement; the possibility of creating a presence that may affirm a participant’s sense of existence seems to engage participants solidly in the process. The research also suggested that for those creating drama on-line the use of a fairly linear narrative structure may still be desirable, and that the more significant experiences of engagement occur when a number of pleasures are experienced in combination. The findings of this research may be of relevance to those interesting in exploring the possibilities of creating drama with young people utilising mediated forms.

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Made available in DSpace on 2014-12-17T15:37:05Z (GMT). No. of bitstreams: 1 RayaneBSN_DISSERT.pdf: 693632 bytes, checksum: a22430e8639b4b9e576ca8573f8e7d9d (MD5) Previous issue date: 2011-08-03 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior / The suprachiasmatic nucleus (SCN) of the anterior hypothalamus, together with the intergeniculate leaflet (IGL) of the thalamus are considered the central components of the circadian timing system (CTS) of mammals. This system is responsible for the generation and regulation of circadian rhythms by establishing a temporal organization of physiological processes and behaviors. The neuronal specific nuclear protein (NeuN) has been widely used as a neuronal marker in several studies. Since glial fibrillary acidic protein (GFAP) is a component of intermediate filaments found in the cytoplasm of astrocytes and is commonly used as a specific marker for these cells. This study aims to identify, in the marmoset, the NeuN immunoreactive neurons and glial cells immunoreactive to GFAP, as well as map the major route of photic synchronization of the STC, retinohypothalamic tract (RHT), and identify the indirect pathway to the SCN and pregeniculate nucleus (PGN) - structure homologous to IGL rodents, using immunohistochemical and cytoarchitectonic techniques. Observed in SCN the presence of neurons immunoreactive to NeuN and terminals immunoreactive subunit b of cholera toxin (CTb), neuropeptide Y (NPY) and serotonin (5- HT). In the PGN noted the presence of the NeuN and NPY immunoreactive neurons and the immunoreactive terminals CTb and 5-HT. Astrocytes are present throughout the extent of the SCN and the PGN this New World primate / O n?cleo supraquiasm?tico (NSQ) do hipot?lamo anterior, juntamente com o folheto intergeniculado (FIG) do t?lamo s?o considerados os componentes centrais do sistema de temporiza??o circadiana (STC) de mam?feros. Tal sistema ? respons?vel pela gera??o e regula??o dos ritmos circadianos estabelecendo uma organiza??o temporal dos processos fisiol?gicos e comportamentos. A prote?na nuclear neuronal espec?fica (NeuN) tem sido amplamente utilizada como um marcador neuronal em diversos estudos. J? a prote?na ac?dica fibrilar glial (GFAP) ? um componente dos filamentos intermedi?rios encontrada no citoplasma dos astr?citos e ? comumente usada como um marcador espec?fico para essas c?lulas. Este trabalho tem como objetivo identificar, no sagui, neur?nios imunorreativos a NeuN e c?lulas gliais imunorreativas a GFAP, bem como mapear a principal via de sincroniza??o f?tica do STC, o trato retinohipotal?mico (TRH), e identificar as vias indiretas para o NSQ e n?cleo pr?-geniculado (NPG) estrutura hom?loga ao FIG dos roedores, utilizando t?cnicas citoarquitet?nica e imunoistoqu?mica. Observamos no NSQ a presen?a de neur?nios imunorreativos a NeuN, bem como terminais imunorreativos a subunidade b da toxina col?rica (CTb), a neuropept?do Y (NPY) e a serotonina (5-HT). J? no NPG notamos a presen?a de neur?nios imunorreativos a NeuN e a NPY e terminais imunorreativos a CTb e a 5-HT. Os astr?citos est?o presentes em toda a extens?o do NSQ e do NPG deste primata do Novo Mundo

Made available in DSpace on 2014-12-17T15:36:41Z (GMT). No. of bitstreams: 1 RuthnaldoRML_TESE.pdf: 6295823 bytes, checksum: 7a96da99950cb2a0427cd0d44be715d0 (MD5) Previous issue date: 2014-08-30 / Conselho Nacional de Desenvolvimento Cient?fico e Tecnol?gico / The pregeniculate nucleus (PGN) of the primate s thalamus is an agglomerate neuronal having a cap shaped located dorsomedially to the main relay visual information to the cerebral cortex, the dorsal lateral geniculate nucleus (GLD). Several cytoarchitectonic, neurochemical and retinal projections studies have pointed PGN as a structure homologous to intergeniculate leaflet (IGL) of rodents. The IGL receives retinal terminals and appears to be involved in the integration of photic and non-photic information relaying them, through geniculo-hypothalamic tract (TGH), to the main circadian oscillator in mammals, the suprachiasmatic nucleus (SCN) of the hypothalamus. Thus, the IGL participates in the control of the biological rhythm by modulating the activity of the SCN. Pharmacological and IGL injury studies conclude that it is critical in the processing of non-photic information which is transmitted to the SCN. Other studies have found that especially neurons immunoreactive to neuropeptide Y (NPY) respond to this type of stimulation, determined by its colocation with the FOS protein. Has not been determined if the PGN responds, expressing the FOS protein, to the non-photic stimulus nor the neurochemical nature of these cells. Thus, we apply a dark pulse in the specifics circadian phases and analyze the pattern of expression of FOS protein in PGN of the marmoset (Callithrix jacchus). We found that in all animals analyzed the FOS expression was higher in the experimental than in the control group. There was a higher expression of FOS when the dark pulse was applied during the subjective day between the groups. Still, a subregion of the PGN, known by immunoreactive to NPY, had a greater number of FOS-positive cells in relation to his other just close dorsal region. Our data corroborate the theory that the PGN and IGL are homologous structures that were anatomically modified during the evolutionary process, but kept its main neurochemical and functional characteristics. However, injury and hodological studies are still needed for a more accurate conclusion / O n?cleo pr?-geniculado (NPG) do t?lamo de primatas ? um aglomerado neuronal, em forma de capuz, localizado dorsomedialmente ao principal retransmissor de informa??es visuais para o c?rtex cerebral, o n?cleo geniculado lateral dorsal (GLD). Diversos estudos citoarquitet?nicos, neuroqu?micos e de proje??es retinianas t?m apontado o NPG como estrutura hom?loga ao folheto intergeniculado (FIG) de roedores. O FIG recebe terminais retinianos e parece estar envolvido na integra??o de informa??es f?ticas e n?o-f?ticas retransmitindo-as, atrav?s do trato geniculohipotal?mico (TGH), ao principal oscilador circadiano em mam?feros, o n?cleo supraquiasm?tico (NSQ) do hipot?lamo. Desse modo, o FIG participa no controle da ritmicidade biol?gica modulando a atividade do NSQ. Estudos farmacol?gicos e de les?o concluem que o FIG ? fundamental no processamento de informa??es n?of?ticas as quais s?o transmitidas ao NSQ. Outros trabalhos verificaram que, especialmente, neur?nios imunorreativos ao neuropept?deo Y (NPY) respondem a esse tipo de est?mulo, determinados por sua co-localiza??o com a prote?na FOS. Ainda n?o foi determinado se o NPG responde, expressando a prote?na FOS, a est?mulos n?o-f?ticos nem tampouco a natureza neuroqu?mica dessas c?lulas. Assim, aplicamos um pulso de escuro em fases circadianas espec?ficas e analisamos o padr?o de express?o da prote?na FOS no NPG do sagui (Callithrix jacchus). Verificamos que em todos os animais analisados a express?o de FOS foi maior em rela??o ao grupo controle. Houve uma maior express?o de FOS quando o pulso de escuro foi aplicado durante o dia subjetivo entre os grupos estudados. Ainda, uma sub-regi?o do NPG, sabidamente imunorreativa a NPY, apresentou um maior n?mero de c?lulas FOSpositivas em rela??o ? sua outra regi?o imediatamente mais dorsal. Os nossos dados corroboram com a teoria de que o NPG e o FIG s?o estruturas hom?logas que se modificaram anatomicamente durante o processo evolutivo, mas mantiveram suas principais caracter?sticas neuroqu?micas e funcionais. No entanto, estudos de les?o e hodol?gicos ainda s?o necess?rios para uma conclus?o mais precisa

A central goal of flavour physics is a precise determination of the elements of the CKM matrix, which quantifies the strength of charged-current weak interactions between quarks. Of particular interest is the angle γ in the 'b-d' unitarity triangle parameterisation of the CKM matrix. One of the most promising methods to determine γ directly is to measure CP violation in interfering B±->DK± decays, where D indicates a coherent superposition of D0 and D0bar, both of which decay to the same final state. When using this method it is essential to determine the hadronic decay parameters of the D precisely in order to reduce the systematic uncertainties on the measurement of γ. One such parameter is the strong-phase difference between D0 and D0bar decays, which must be accurately known across the entire kinematic phase space. In this thesis we present measurements related to the determination of γ at both the CLEO-c experiment at Cornell University and the LHCb experiment at CERN. Firstly, we describe a model-independent determination of the D->KsKK strong-phase difference using 818pb-1 of quantum-correlated D0-D0bar data collected by CLEO-c at the ψ(3770) resonance. We reconstruct D->KsKK decays tagged with a variety of final states. By studying these decays we determine the weighted cosine and sine of the strong-phase difference in bins across the Dalitz plane. We run simulations to estimate the impact of these measurements on a determination of γ using B±->D(KsKK)K± decays. The resulting uncertainty on γ due to the CLEO-c inputs is between 3.2° and 3.9°, depending on how the Dalitz plane is binned. Furthermore, we present a model-independent measurement of the CP content of the decay D0->KsKK in the kinematic region of the φ->KK resonance. The fraction of CP-odd events in this region is 0.76 or higher at the 90% C.L. We also present an analysis of data recorded by LHCb in 2010, corresponding to an integrated luminosity of 36.5pb-1. We reconstruct the decays B±->D(Kπ)h± and B±->D(KK)h±, where h± indicates either K± or π±. Although there are not enough events in this dataset to measure γ, we are able to measure other observables related to CP violation in the B±->Dh± system. We measure B(DK,Fav)/B(Dπ,Fav), the ratio of the branching fraction of B±->D(Kπ)K± to that of B±->D(Kπ)π±, to be 0.066 ± 0.005 ± 0.004, and B(DK,CP)/B(Dπ,CP), the ratio of the branching fraction of B±->D(KK)K± to that of B±->D(KK)π±, to be 0.093 ± 0.019 ± 0.005. We determine several CP asymmetries: A(CP+,DK), the CP asymmetry in B±->D(KK)K± decays, is measured as 0.06 ± 0.17 ± 0.07; A(CP+,Dπ), the CP asymmetry in B±->D(KK)π± decays, is found to be 0.009 ± 0.042 ± 0.011; and A(Fav,DK), the CP asymmetry in B±->D(Kπ)K± decays, is measured as -0.109 ± 0.085 ± 0.019. Finally we calculate R(CP+), the ratio of the branching fraction of B±->D(KK)K± to that of B±->D(Kπ)K±, to be 1.41 ± 0.31 ± 0.11. These results indicate that LHCb is in a strong position to make a world-leading measurement of γ with a larger data sample.

Submitted by PPG Odontologia (odontologia-pg@pucrs.br) on 2018-03-09T17:42:57Z No. of bitstreams: 1 RICARDO_LOSEKANN_PAIVA_TES.pdf: 1108231 bytes, checksum: a32ed38f5354b3e7c925afdd81c861e4 (MD5) / Approved for entry into archive by Tatiana Lopes (tatiana.lopes@pucrs.br) on 2018-03-15T13:00:03Z (GMT) No. of bitstreams: 1 RICARDO_LOSEKANN_PAIVA_TES.pdf: 1108231 bytes, checksum: a32ed38f5354b3e7c925afdd81c861e4 (MD5) / Made available in DSpace on 2018-03-15T13:37:24Z (GMT). No. of bitstreams: 1 RICARDO_LOSEKANN_PAIVA_TES.pdf: 1108231 bytes, checksum: a32ed38f5354b3e7c925afdd81c861e4 (MD5) Previous issue date: 2017-11-29 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Oral cytopathology may be used to monitor individuals exposed to risk factor for oral cancer. In this context, the study of field cancerization, a phenomenon involved in the initial stages of oral carcinogenesis, has gained relevance for the establishment of biomarkers that may identify individuals exposed to carcinogens with the greatest risk for developing oral cancer. The first article of this study aimed to compare cytopathological and histopathological characteristics of the clinically normal mucosa adjacent to oral squamous cell carcinoma. The nuclear area of cells obtained by cytological smear of this region was also analyzed and compared to that of individuals without lesions but exposed to smoking and/or alcohol and of patients not exposed to these risk factors. Ninety patients of both sexes over 40 years old were included. In patients with carcinoma, in addition to exfoliative cytology, tissue was obtained from the area adjacent to the tumor for histopathological examination. In smears stained with Papanicolaou, the nuclei of 50 intermediate cells were measured. Both histological sections and cytological smears were classified as low or high-risk. The sensitivity, specificity and accuracy of the cytopathological diagnosis in relation to the histopathological diagnosis, considered the gold standard, were 100, 75 and 75.86%, respectively. The mean nuclear area was significantly lower (p<0.05) in the patients not exposed to the risk factors in relation to the others. The cyto-histopathological comparison of the area adjacent to oral cancer showed good sensitivity, specificity and accuracy. In conclusion, this article demonstrated that nuclear area can be used to detect early cellular changes in the oral mucosa exposed to carcinogens and that mean percentage of nuclei larger than 100 ?m2 is the most indicated method for the assessment of these changes. The second article aimed to assess genetic damage and cell proliferation rate in the field of cancerization, i.e., the clinically normal mucosa adjacent to oral carcinoma. Cytologic smears from the same scrapes used in the first article were stained with silver and with the Feulgen reaction. The mean number of AgNORs/nucleus and micronuclei (MN) was significantly higher (p<0.05) in the Tobacco/Alcohol and Oral Cancer Groups than in the Control Group. Conversely, the mean number of NBUDs was higher in the Control Group compared with the other groups. The number of AgNORs/nucleus and MN/1,000 cells provide evidence of initial oral carcinogenesis at field cancerization areas. Cutoff values for inclusion of individuals exposed to carcinogens in longitudinal monitoring were ? 3.38 AgNORs/nucleus and/or ? 3 MN/1,000 cells. A prospective model including the biomarkers assessed in this study was proposed. / A citopatologia bucal pode ser aplicada como m?todo de monitoramento em indiv?duos expostos a fatores de risco ao c?ncer de boca. O campo de canceriza??o, representando as etapas iniciais da carcinog?nese bucal, torna-se uma ?rea atrativa ao estudo de biomarcadores que poder?o ser utilizados para identificar indiv?duos expostos a carcin?genos com maior risco ao desenvolvimento do c?ncer bucal. O primeiro artigo deste estudo visou comparar as caracter?sticas citopatol?gicas e histopatol?gicas da mucosa clinicamente normal adjacente ao carcinoma espinocelular bucal. Al?m disso, a ?rea nuclear das c?lulas obtidas desta regi?o, por meio de esfrega?o citol?gico, foi mensurada e comparada ? das c?lulas de indiv?duos sem les?o expostos ao fumo e/ou ?lcool e a de pacientes n?o expostos a estes fatores de risco. Foram inclu?dos 90 pacientes de ambos os sexos com idade superior a 40 anos. Nos pacientes com carcinoma, al?m da citologia esfoliativa, foi obtido material para exame histopatol?gico da ?rea adjacente ao tumor. Nos esfrega?os, corados com Papanicolaou, foram mensurados os n?cleos de 50 c?lulas intermedi?rias. Tanto os cortes histol?gicos, quanto os esfrega?os citol?gicos foram classificados em baixo ou alto risco. Ao associar as caracter?sticas citopatol?gicas e histopatol?gicas, verificou-se sensibilidade, especificidade e acur?cia de 100%, 75% e 75,86%, respectivamente. A m?dia da ?rea nuclear foi menor no grupo n?o exposto ao fumo e ao ?lcool, com diferen?a significativa (p<0,05) em rela??o aos demais. A associa??o cito-histopatol?gica da ?rea adjacente ao c?ncer bucal apresentou boa sensibilidade, especificidade e acur?cia. Al?m disso, constatou-se que a ?rea nuclear ? pass?vel de ser utilizada para detectar altera??es celulares precoces na mucosa bucal exposta a carcin?genos, sendo a m?dia percentual de n?cleos com mais de 100 ?m2 o m?todo de avalia??o mais indicado. O segundo artigo objetivou avaliar o dano gen?tico e a velocidade de prolifera??o celular no campo de canceriza??o, ou seja, na mucosa clinicamente normal adjacente ao c?ncer bucal. Os esfrega?os citol?gicos foram corados com a t?cnica das AgNORs e rea??o de Feulgen, utilizando o mesmo raspado citol?gico obtido no primeiro artigo. A m?dia das AgNORs/n?cleo e do micron?cleo (MN) dos grupos fumo/?lcool e carcinoma bucal foi superior, com diferen?a estatisticamente significativa (p<0,05) em rela??o ao grupo controle. Este, por sua vez, obteve m?dia superior de bot?es nucleares (NBUDs) em rela??o aos grupos fumo/?lcool e carcinoma bucal. Ambos os marcadores, n?mero das AgNORs/n?cleo e MN, evidenciaram a fase inicial da carcinog?nese bucal, representada no campo de canceriza??o. Valores iguais ou superiores a 3.38 AgNORs/n?cleo e/ou 3 MN/1000 c?lulas foram identificados como ponto de corte ideal para incluir um indiv?duo exposto a carcin?genos no monitoramento longitudinal. Um modelo prospectivo dos marcadores foi sugerido.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-08-07T01:02:49Z No. of bitstreams: 1 DISSERTA??O- LEONARDO DA CUNHA MENEZES SOUZA.pdf: 2029455 bytes, checksum: 2374a0c5996ead5ddad034150d2e36b7 (MD5) / Made available in DSpace on 2015-08-07T01:02:49Z (GMT). No. of bitstreams: 1 DISSERTA??O- LEONARDO DA CUNHA MENEZES SOUZA.pdf: 2029455 bytes, checksum: 2374a0c5996ead5ddad034150d2e36b7 (MD5) Previous issue date: 2013-09-30 / Funda??o de Amparo ? Pesquisa do Estado da Bahia - FAPEB / The use of anabolic androgenic steroids (AAS) has grown among practitioners of recreational bodybuilding, with significant contributions of "Designer Steroids" (DS), EAA designed aiming muscle hypertrophy in healthy subjects. The abusive use of AAS in general is associated with adverse effects, one of the most worrisome is cancer development. Given that cancer is a genetic disease resulting from changes in genes critical in maintaining genomic stability and in the control of proliferation and differentiation, biomarkers able to identify these changes can take predictive value and contribute to reducing the high rates of morbidity and mortality by this disease. The aim of this study was to evaluate the effectiveness of the Cytokinesis Block Micronucleus Test (CBMN) in human lymphocytes in identifying risk groups for cancer development in users of AAS. Was collected 5ml of blood from 15 AAS users bodybuilders (G1), 20 nonusers bodybuilders (G2) and 20 nonusers sedentary (G3). Lymphocytes were cultured with blocking of cytokinesis and subsequent processing for making slides. MN analysis was performed on a minimum of 1000 binucleated lymphocytes. The occurrence of MN was significantly higher (p <0.05) in individuals of G1 compared to G2 and G3. The results indicate the sensitivity of CBMN in human lymphocytes in the identification of chromosomal damage in consequence of AAS using, however, further studies are needed to determine their potential to predict the cancer risk in users of AAS. / O uso de esteroides anabolizantes androg?nicos (EAA) tem crescido entre praticantes recreativos de muscula??o, com contribui??es significativas dos Designer Steroids (DS), EAA projetados para fins de hipertrofia muscular em indiv?duos saud?veis. O uso abusivo de EAA em geral est? associado a efeitos adversos, sendo um dos mais preocupantes o desenvolvimento de c?ncer. Tendo em vista que o c?ncer ? uma doen?a gen?tica resultante de altera??es em genes cr?ticos na manuten??o da estabilidade gen?mica e do controle da prolifera??o e diferencia??o, biomarcadores capazes de identificar estas altera??es podem assumir valor preditivo e contribuir para diminui??o das altas taxas de morbidade e mortalidade por esta doen?a. O objetivo deste estudo foi avaliar a efic?cia do Teste de Micron?cleo com Bloqueio da Citocinese (MNCtB) em linf?citos humanos na identifica??o de grupos de risco para o desenvolvimento de c?ncer em usu?rios de EAA. Foram coletados 5ml de sangue de 15 usu?rios de EAA praticantes de muscula??o (Grupo I), 20 n?o usu?rios praticantes de muscula??o (Grupo II) e 20 n?o usu?rios sedent?rios (Grupo III). Foi realizada cultura de linf?citos com o bloqueio da citocinese e posterior processamento para confec??o de l?minas. A an?lise de MN foi realizada em um m?nimo de 1000 linf?citos binucleados. A ocorr?ncia de MN foi significativamente maior (p<0,05) nos indiv?duos do Grupo I comparados aos do Grupo II e Grupo III. Os resultados apontam para a sensibilidade do MNCtB na identifica??o de danos cromoss?micos decorrentes do uso de EAA. Estudos subsequentes para determinar seu potencial preditivo para o risco de c?ncer em usu?rios de EAA s?o necess?rios.

Submitted by Verena Bastos (verena@uefs.br) on 2015-10-08T12:18:43Z No. of bitstreams: 1 Jeanderson_Disserta??o_vers?o final.pdf: 369468 bytes, checksum: 851a8492d73fc0106bfe7784166fdf98 (MD5) / Made available in DSpace on 2015-10-08T12:18:43Z (GMT). No. of bitstreams: 1 Jeanderson_Disserta??o_vers?o final.pdf: 369468 bytes, checksum: 851a8492d73fc0106bfe7784166fdf98 (MD5) Previous issue date: 2013-03-25 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Exposure to genotoxic agents induces changes in the DNA molecule to commit that genes involved with the repair mechanisms and the control of cell proliferation and differentiation pathways or genes associated with apoptosis can lead to cancer development. Among the many chemicals that have been identified as mutagenic action, include the Androgenic Steroids (AAS), hormones widely used in the search for improved physical performance and increased muscle mass. Objective: In this context, the aim of the development of this study was to evaluate the potential of androgenic anabolic steroid nandrolone decanoate, testosterone propionate and testosterone cypionate to induce chromosome damage, apoptosis and necrosis, using the micronucleus test in exfoliated cells from the oral mucosa of users of AAS with a view to its application as a tool in cancer prevention. Method: The study sample consisted of 55 volunteers, male, divided into two (02) groups, matched for age: 25 subjects (G1) users of nandrolone decanoate, testosterone propionate and testosterone cypionate (alone or simultaneously ) and 30 subjects in the control group (G2). The collection methodology and cytological analysis followed the protocol of Tolbert et al. (1992) and Thomas et al. (2009), which includes, in addition to micronuclei, the computation of degenerative nuclear changes indicative of apoptosis (cariorr?xis, condensed chromatin and pyknosis) and necrosis (karyolysis, cariorr?xis, condensed chromatin and pyknosis). Statistical analysis of the endpoints analyzed (micronucleus, cariorr?xis, condensed chromatin, karyolysis, pyknosis and broken eggs) was performed using the conditional test to compare proportions in situations of rare events. Results: Statistical analysis revealed no significant difference in the occurrence of micronuclei, karyolysis and broken eggs between groups. The occurrence of apoptosis was significantly greater in cells from control individuals. Conclusion: The results show inhibition of apoptosis induced by EAA, suggesting that the described association between the use of these substances and the carcinogenic process can be permeated by this mechanism. / A exposi??o a agentes genot?xicos induz altera??es na mol?cula do DNA que ao comprometerem genes envolvidos com os mecanismos de reparo e com o controle da prolifera??o e diferencia??o celular ou genes associados ?s vias de apoptose, podem levar ao desenvolvimento de c?ncer. Entre os muitos agentes qu?micos que t?m sido identificados como de a??o mutag?nica, incluem-se os Ester?ides Anabolizantes Androg?nicos (EAA), horm?nios amplamente utilizados na busca da melhoria do desempenho f?sico e aumento da massa muscular. Objetivo: Neste contexto, objetivou-se com o desenvolvimento do presente estudo, avaliar o potencial dos ester?ides anabolizantes androg?nicos decanoato de nandrolona, propionato de testosterona e cipionato de testosterona em induzir danos cromoss?micos, apoptose e necrose, atrav?s do uso do Teste de Micron?cleo em c?lulas esfoliadas da mucosa oral de usu?rios de EAA com vista ? sua aplica??o como ferramenta na preven??o do c?ncer. M?todo: A amostra do estudo foi composta por 55 volunt?rios, do sexo masculino, distribu?dos em dois (02) grupos, pareados por idade: 25 indiv?duos (G1) usu?rios de decanoato de nandrolona, propionato de testosterona e cipionato de testosterona (isoladamente ou simultaneamente) e 30 indiv?duos no grupo controle (G2). A metodologia de coleta e an?lise citol?gica seguiu o protocolo de Tolbert et al. (1992) e Thomas et al. (2009), que inclui, al?m de micron?cleos, o computo de altera??es nucleares degenerativas indicadoras de apoptose (cariorr?xis, cromatina condensada e picnose) e necrose (cari?lise, cariorr?xis, cromatina condensada e picnose). A an?lise estat?stica dos endpoints analisados (micron?cleo, cariorr?xis, cromatina condensada, cari?lise, picnose e broken eggs) foi realizada com o uso do teste condicional para compara??o de propor??es em situa??es de eventos raros. Resultados: A an?lise estat?stica revelou que n?o houve diferen?a significativa na ocorr?ncia de micron?cleo, cari?lise e broken eggs entre os grupos. A ocorr?ncia de apoptose foi, significativamente, maior em c?lulas dos indiv?duos do grupo controle. Conclus?o: Os resultados obtidos mostram inibi??o da apoptose induzida pelo uso de EAA, sugerindo que a associa??o descrita entre uso destas subst?ncias e o processo carcinog?nico possa ser permeada por este mecanismo.