M.Comm. / The Taxi Recapitalisation Programme (TRP) was first announced by the Government in 1999. It was originally recommended by the National Taxi Task Team (NTTT) as a strategy to transform and regulate the South African Combi-Taxi Industry (SACTI) into a “new” taxi industry. Its aims were to improve the quality of combi-taxi transport in South Africa by taking a number of steps, including institutionalising the industry, changing the licencing system, regulating the industry and replacing old vehicles with new ones. Since that time, however the TRP has been plagued by controversy and its implementation has fallen behind schedule. The study‟s key research aim has been to investigate and assess perceptions of taxi associations (taxi owners) with regard to the TRP. The study took place in Temba, near Hammanskraal, approximately 55 kilometres north of Pretoria. Temba is taken as a “microcosm” of SACTI in that it can be regarded as typical of many other areas in the country. A background is provided to the regulatory legislation of the public transport system of the apartheid Government and the general historical and economic development of the SACTI prior to 1994. It deals with the periods from 1930-1976, 1977-1985 and 1986-1993, and then explores the legislative and policy framework of the public transport system of the post-1994 democratic government up to 2008. It introduces the NTTT and its recommendations before analysing the economic effects likely to be felt by taxi associations following implementation of the TRP. The study then presents the results of a brief demographic profile of a sample of taxi owners and operators in the Temba area and identifies a number of factors that influence their attitudes towards the industry in general and their perceptions of the TRP in particular. It assesses the progress made with the implementation of the TRP since its inception and identifies critical issues that have delayed the project. Having been scheduled to span the five years from 2001 to 2005, the date of completion of the TRP was later extended to 2007. By 2010 the project was still only partially complete. Due to repeated delays, the project appeared to be running aground and was perceived by many as a fruitless and wasteful exercise. Indeed, these delays have undermined the goals of transport regulation which, inter alia, were intended to promote the welfare of the public (safety, security, satisfaction, health and protection of property) and to improve the quality of public transport generally. By linking the demographic profile of the taxi owners with the various perceptions, the study has been able to draw conclusions and make findings intended to contribute towards the successful implementation of the TRP and thereby assist SACTI to play a more effective role in the overall transport situation in South Africa. The study concludes that taxi associations in Temba have perceived the TRP to be a good proposal but difficult to implement. It therefore makes a number of recommendations that will hopefully assist in the transformational process of changing SACTI “old” taxi industry into a “new” taxi industry, and so enhance the economic strength of taxi owners and operators, as well as improve the welfare of the users of taxi transport services, not only in Temba but throughout the country.

The aim of the master thesis was to assess volume of disposable calcium in the ponds sediments and acid neutralization capacity of water, pH of water, oxygen saturation and zooplankton abundace during vegetation period. Following to the indicators was found out ponds production as well. Studies took place on 6 ponds (Byňovský, Dolní Velký, Kačák, Nakolický, Nový v Oboře, Žár) of company Fyshery New Castles s. r. o. For control was chosen pond Farský which is managed by Czech Fishing Union. Volume of disponable calcium in ponds sediments was transferred with infusion of 0,1 M H2CO3 . Volume of calcium was determined with chelatometric titration method. In 2013 the samples of sediments were collected before vegetation period. During the next season were determined samples of oxygen, ANK4,5 of water, pH of water and zooplankton which was sorted according its size (<500m and >500 m. At all monitored ponds was found out close relation between to volume of Ca2+ in pond sediment and pH of water, ACN4,5 of sediments and also with ACN4,5 of water during vegetation period. The highest average values of ACN4,5 of water were found out on pond Kačák (0,93 mmo.l-1), Žár (1,01 mmol.l-1) and Nový v Oboře (0,83 mmol.l-1), which significantly correlated with reserve of pond calium during dormancy. Was found out significant corelation between total growth of fish and coarse zooplankton. More significant relation was found out between total growth of fish and total abundance of zooplankton but between natural growth of fish and total abundance of zooplankton as well. The highest growths were found out on Ponds (Kačák, Žár), whose samples showed the biggest abundance of zooplankton from all monitored ponds and vice versa it wasn't confirmed relation between ACN4,5 of water during vegatation period and pH of water, abundance of coarse zooplankton and oxygen regime of water.

Global energy demand is increasing every year dramatically, which results global warming due to increased greenhouse gas emissions. So, to be environmentally friendly and sustainable, Sweden’s energy policy targets to reach 100 % renewable energy share in 2040. Already, Sweden achieved 50 % renewable energy share in 2012 which was targeted to achieve by 2020. Integration of solar heating system in residential sector, for space heating (SH) and domestic hot water (DHW), was one of the reasons for this achievement. Considerably, several numbers of solar heating systems were installed in houses until 2011. Thus, solar heating system could be a step towards to reach 2040s target. In 2011, detached Swedish house was installed with solar combi-system (SCS) which was designed to use 53% of solar energy. The main aim of this thesis is to analyze the thermal performance of SCS and correlation with its simulated system performance which is done in five steps.First step is the collection of measured data from the system for specific period one year from April 2019 to March 2020 and determined other unknown energy values in that data. In the second step, unknown data are calculated using scientific equations with information from users and reliable assumptions. Consequently, the analysis of measured data shows huge uncertainties. It follows to the third step as sensitivity analysis to enhance the assumption values. Thus, analyzed results are tabulated with reliable parameters and solar fraction (SF) of the measured data is calculated. In the fourth step, SH of the house is considered as reference to build a system model in Polysun simulation software, and the modelled results are verified with analyzed results. In the final step, system model is simulated by changing one-year weather profile to 10 years average weather profile. Thus, analyzed results and simulated results are compared to evaluate the performance. The real system has the SF of 52 % after sensitivity analysis whereas the simulated system showed the SF with percentage difference around 15 %.

Global energy demand is increasing every year dramatically, which results global warming due to increased greenhouse gas emissions. So, to be environmentally friendly and sustainable, Sweden’s energy policy targets to reach 100 % renewable energy share in 2040. Already, Sweden achieved 50 % renewable energy share in 2012 which was targeted to achieve by 2020. Integration of solar heating system in residential sector, for space heating (SH) and domestic hot water (DHW), was one of the reasons for this achievement. Considerably, several numbers of solar heating systems were installed in houses until 2011. Thus, solar heating system could be a step towards to reach 2040s target. In 2011, detached Swedish house was installed with solar combi-system (SCS) which was designed to use 53% of solar energy. The main aim of this thesis is to analyze the thermal performance of SCS and correlation with its simulated system performance which is done in five steps.First step is the collection of measured data from the system for specific period one year from April 2019 to March 2020 and determined other unknown energy values in that data. In the second step, unknown data are calculated using scientific equations with information from users and reliable assumptions. Consequently, the analysis of measured data shows huge uncertainties. It follows to the third step as sensitivity analysis to enhance the assumption values. Thus, analyzed results are tabulated with reliable parameters and solar fraction (SF) of the measured data is calculated. In the fourth step, SH of the house is considered as reference to build a system model in Polysun simulation software, and the modelled results are verified with analyzed results. In the final step, system model is simulated by changing one-year weather profile to 10 years average weather profile. Thus, analyzed results and simulated results are compared to evaluate the performance. The real system has the SF of 52 % after sensitivity analysis whereas the simulated system showed the SF with percentage difference around 15 %.

L’émergence de nouveaux contaminants dans les eaux usées requiert le développement de nouvelles techniques. En effet, les traitements classiques des stations d’épuration des eaux usées laissent entrer dans les matrices environnementales de nombreux contaminants organiques de faibles concentrations tels que les produits pharmaceutiques. Nous avons donc étudié l’élimination d’une variété de pharmaceutiques, représentatifs de leur classe, ou bien présentant une forte occurrence, ou encore des composés récalcitrants. Parmi ces pharmaceutiques on retrouve des anti-inflammatoires non stéroïdiens (acétaminophène, naproxène, acide méfénamique, kétoprofène, indométacine, diclofénac), un stimulant (caféine) deux antibiotiques (ciprofloxacine et triméthoprime), un anticonvulsif et régulateur de l’humeur (carbamazépine), un anxiolytique (diazépam) et deux fibrates (fénofibrate et bézafibrate). Parmi les techniques novatrices permettant de réaliser ce type d’élimination on retrouve certaines enzymes oxydatives qui sont capables de transformer de nombreux contaminants organiques que l’on retrouve dans les eaux usées. L’utilisation de trois enzymes de ce type, la laccase, la versatile peroxydase et la glucose oxydase, dans différentes combinaisons, a permis d’obtenir une élimination satisfaisante de la plupart des pharmaceutiques auxquels nous nous sommes intéressés, avec une efficacité optimale pour la combinaison des trois enzymes. Partant de ce constat, une combinaison plus stable de ces trois enzymes a été produite par une technique de co-aggrégation permettant de les insolubiliser tout en les regroupant par réticulation. Ceci facilite la réutilisation de ces biocatalyseurs, et augmente leur stabilité, ce qu’une caractérisation du biocatalyseur a permis de vérifier. Le biocatalyseur a alors pu être testé pour le traitement d’un cocktail des produits pharmaceutiques précédemment énoncés et a permis de réaliser une élimination de plus de 60 % de la plupart des composés dans des conditions qui ont été optimisées. Testé dans des eaux résiduaires urbaines prélevées à l’affluent de la station d’épuration de Magog (Québec), le biocatalyseur a permis une élimination de l’ordre de 25 % pour des concentrations très faibles (ppb) en acétaminophène.

Na produção de suco de uva, a adição de preparados enzimáticos durante a maceração, antes da prensagem, é um pré-requisito para a obtenção de rendimentos satisfatórios de suco, além de melhorar a qualidade organoléptica, promovendo uma maior extração de cor e compostos bioativos. Uma segunda aplicação enzimática pode ser realizada após o processo de prensagem no suco ainda turvo, buscando reduzir essa turbidez, o que facilitará os processos de clarificação e filtração posteriores, aumentando a produtividade dessas etapas. Porém, existe um grande número de enzimas comerciais disponíveis para a aplicação na indústria de sucos, sendo assim, é importante avaliar o potencial de cada preparado enzimático para uma utilização correta. Desta forma, o objetivo deste trabalho foi avaliar a influência da composição dos diferentes preparados enzimáticos na extração, qualidade e compostos bioativos do suco de uva elaborado com a variedade Concord. Primeiramente, o efeito de oito diferentes preparados enzimáticos foi avaliado para extração e qualidade do suco de uva. Subsequentemente, os dois preparados enzimáticos que se destacaram (Pectinex® Ultra Clear e Lallzyme® Beta), foram utilizados de forma sinérgica em um planejamento experimental, buscando encontrar a condição ótima de tempo, temperatura, concentração de enzima e proporção de Pectinex® Ultra Clear/Lallzyme® Beta, que pudesse maximizar a extração do suco de uva. E, por fim, foram obtidos agregados enzimáticos entrecruzados (CLEAs), como forma de imobilização das diferentes enzimas encontradas nos preparados enzimáticos para a clarificação do suco de uva. Os experimentos foram realizados no Laboratório de Biocatálise e Tecnologia Enzimática do ICTA/UFRGS. As uvas utilizadas nos experimentos foram da variedade Concord fornecida pela empresa Vitivinícola Jolimont, de Canela, RS. As preparações enzimáticas utilizadas foram caracterizadas em relação a cinco atividades enzimáticas, sendo elas, pectinase total (PE), poligalacturonase (PG), pectina liase (PL), pectina metil esterase (PME) e celulase (CE). Desta mesma forma, os combi-CLEAs foram caracterizados em relação as suas atividades enzimáticas, além disso, sua estabilidade, reutilização e pH e temperatura ótima foram avaliados. Os compostos bioativos foram identificados e quantificados por HPLC-DAD-MS (cromatografia líquida de alta eficiência - detector de arranjo de diodos - espectro de massa) e a atividade antioxidante foi avaliada através dos métodos de ABTS e de capacidade redutora. Rendimentos superiores de suco foram obtidos com os preparados que apresentaram altas concentrações de PG e PME ou PL. Quando, o preparado Pectinex® Ultra Clear (PUC) foi utilizado, observou-se um aumento de 8,7% na quantidade de suco extraído em comparação com o controle. Já os maiores valores de compostos bioativos foram encontrados com os preparados que apresentaram em sua composição uma maior atividade CE (Lallzyme® Beta, LB). Entretanto, quando PUC e LB foram adicionados em conjunto, maiores valores de rendimento e compostos bioativos foram encontrados. Já os combi-CLEAs, se mostraram como uma ótima forma de enzima imobilizada para a clarificação do suco de uva. Dentre os combi-CLEAs produzidos, destacaram-se o combi-CLEA-BSA devido à melhor atividade enzimática e maior estabilidade térmica (3 vezes mais que a enzima solúvel), podendo ser reutilizados por 6 ciclos com conversão total do substrato em produto. Além disso, o combi-CLEA-BSA melhorou a clarificação do suco, obtendo uma redução da turbidez de 56.7 % em 1 h. Por fim, pode-se afirmar que o trabalho atingiu melhorias produtivas e qualitativas ao suco de uva através da tecnologia enzimática. / In grape juice production, the addition of enzyme preparations during the maceration prior to pressing is a prerequisite for obtaining satisfactory juice yield, besides it improves the organoleptic quality, improving color and bioactive compounds extraction. A second enzyme application may be performed after the pressing process in cloudy juice, in order to reduce this turbidity, facilitating clarification and filtration processes. However, there are a great number of commercial enzymes available to the application on the fruit juice industry, so it is important to know the ability of each enzyme preparation for correct use. Thus, the aim of this study was to evaluate the influence of the composition of the different enzymatic preparations in the extraction, quality and bioactive compounds of grape juice from Concord variety. Firstly, the effect of eight different enzyme preparations was evaluated for extraction and quality of grape juice. Subsequently, the best enzyme preparations (Pectinex® Ultra Clear and Lallzyme® Beta) were used in an experimental design to find the optimal time, temperature, enzyme concentration and ratio of Pectinex® Ultra Clear/Lallzyme® Beta which could maximize the extraction of grape juice. Finally, cross-linked enzyme aggregates (CLEAs) were obtained as a way to immobilize the different enzymes found in the enzyme preparations for grape juice clarification. The experiments were performed at the Laboratory of Biocatalysis and Enzyme Technology, ICTA/UFRGS. The grapes (Concord variety) were provided by Vitivinícola Jolimont, Canela, RS. The enzymatic preparations were characterized through of five enzymatic activities, being total pectinase (PE), polygalacturonase (PG), pectinlyase (PL), pectin methyl esterase (PME) and cellulase (CE). In the same way, the combi-CLEAs were characterized regarding their enzymatic activities, thermal, pH and operational stabilities, and optimal pH and temperature. The bioactive compounds were identified and quantified by HPLC-DAD-MS (High Performance Liquid Chromatography - Diode Array Detector – Mass Spectrum) and the antioxidant activity was performed by the method of ABTS and reducing capacity. High juice yield was obtained with the preparations that presented higher concentrations of PG, PME or PL activities. The Pectinex® Ultra Clear, when compared with the control, provided an increase of 8.7 % in the amount of juice extracted. On the other hand, the highest values of bioactive compounds were found for preparations with higher CE activity (Lallzyme® Beta). However, when PUC and LB were added together, higher values for yield and bioactive compounds were found. Already, regarding the combi-CLEAs, it appears to be a potential immobilized enzyme for clarification of grape juice. Among the combi-CLEAs obtained, combi-CLEAs-BSA presented the best enzyme activities, higher thermal stabilities (3 times more than the soluble enzyme) and it can be reused for 6 cycles with a total conversion of substrate to product. Furthermore, the combi-CLEA-BSA improved the juice clarification, obtaining 56.7 % in turbidity reduction in 1 h. Finally, it can be concluded that the work reached productive and qualitative improvements for grape juice through enzymatic technology.

Estudos vem mostrando resultados promissores na utilização da tecnologia de ultrassom para a produção enzimática de biodiesel. Estes são atribuídos ao fenômeno de cavitação gerado pelo equipamento de ultrassom, o qual promove um aumento da miscibilidade entre os reagentes, melhorando a transferência de massa e a taxa da reação, proporcionando reações mais rápidas, bem como um menor consumo de reagentes. Sendo assim, o objetivo deste trabalho foi otimizar as condições da reação de transesterificação do óleo de soja para a produção de biodiesel, utilizando o conceito de combi-lipase em um reator enzimático assistido por ultrassom. Foram otimizadas as condições do sistema de ultrassom: amplitude (30-70 %), pulso (30-70 %) e tempo de pulso (5-15 s), através de um delineamento composto central rotacional (DCCR). Os parâmetros reacionais: concentração de biocatalisador (5, 15 e 25 % em relação a massa de óleo) e razão molar do substrato (3:1, 6:1 e 9:1) foram estudados, assim como a influência do uso do solvente terc-butanol e de um sistema de agitação combinado ao ultrassom, na conversão de ésteres etílicos. Avaliou-se também a eficiência do combi-lipase (75 % Novozym 435 + 10 % Lipozyme TL-IM and 15 % Lipozyme RM-IM) frente ao uso dos biocatalisadores individualmente. Obteve-se como condições ótimas para o sistema de ultrassom: 30 % de amplitude, 50 % de pulso e 15 s de tempo de pulso, 15 % de enzima em relação a massa de óleo e uma razão molar de 3:1 etanol:óleo de soja Os rendimentos de conversão de ésteres etílicos na ausência do solvente terc-butanol foram similares aos resultados obtidos na presença deste reagente, sugerindo que a tecnologia de ultrassom é capaz de eliminar a necessidade do uso de solventes em reações de transesterificação enzimática. Entretanto, o uso combinado de um sistema de agitação com o ultrassom, reduziu o rendimento da reação. Em relação às enzimas, o combi-lipase mostrou melhores resultados para a síntese enzimática de ésteres etílicos, do que o uso das enzimas individualmente. Utilizando as condições ótimas avaliadas neste estudo e o conceito de combi-lipase em um reator assistido por ultrassom, obteve-se uma conversão de ésteres etílicos de aproximadamente 75 % em 5 horas de reação. / Studies have been showing promising results for the use of ultrasonic technology in enzymatic production of biodiesel, which are attributed to the cavitation phenomenon generated by the ultrasound equipment, that promotes increased miscibility between the reactants, improving mass transfer and reaction rate, providing faster reactions as well as a lower consumption of reagents. The aim of this work was to optimize ultrasonic (amplitude, pulse and time pulse) and reaction (molar ratio and enzyme concentration) parameters, as well as the influence of solvent (tert-butanol) and ultrasound combined with mechanical stirring, on the transesterification of soybean oil catalyzed by combi-lipase biocatalyst. It was also evaluated the efficiency of the combi-lipase (75 % Novozym 435 + 10 % Lipozyme TL-IM and 15 % Lipozyme RM-IM), compared to individual lipases. The optimum conditions for transesterification reaction, were observed being: enzyme concentration, 15 % (by oil mass); ethanol:oil molar ratio, 3:1; ultrasonic amplitude 30 %, pulse, 30 % and time pulse, 15 s. The yields of conversion of ethyl esters with and without solvent were very similar, indicating that ultrasonic technology is able to supply the need of solvents in enzymatic transesterification reactions. The combined use of a mechanical stirring system with ultrasound, reduced the yields of conversion of this reaction, while the combi-lipase showed better results than the use of individual lipases for soybean oil transesterification. Using the optimum conditions evaluated in this study and the concept of combi-lipase in an ultrasonic-assisted batch reactor, led to conversions of ethyl esters of about 75% in 5 hours.

Today’s demand on forklift trucks performance and efficiency is high. The productivity is important but also the experience while handling the forklift. The handling has to be simple and genuine to make the driver feel confident and safe. To achieve high performance steering in articulated trucks, a hydraulic power system is often used.Simulation software are a powerful tool in development processes. The program gives the industry a possibility to develop, analyze and evaluate constructions and models more efficient.The purpose of this master thesis is to identify and increase the knowledge about the main challenges in the hydraulic steering system in an articulated forklift. The hydraulic system has been modelled in the simulation software Hopsan and validated against data from measurements performed on the forklift. The different challenges have been identified based on tests and the simulation results. For a deeper understanding of the system a literature study, mainly about the key components, has been done during the master thesis. A number of suggestions for improvement have been developed with focus on increasing the steering performance. The concepts and ideas have been evaluated and tested in the simulation model.The project resulted in a validated simulation model of the articulation and a number of suggested improvements on the hydraulic steering system.

The present master thesis was done during the spring of 2018. A cottage located in Vifors is studied with regard to its heating requirements. At the time of the study, the house could not be inhabited the whole year because there was no tap hot water available and the space heating demand was covered by electricity. Thus, an alternative heating supply is required to be developed. As a strategic prerequisite, the solution should be achieved considering both solar thermal collectors and a heat pump.First, the characteristics of the building were collected/determined in order to obtain the total heating demand per month and hence annually. Parameters such as the U-values, roof orientation, room dimensions, ventilation rates and internal gains were required to configure the building model in the software IDA ICE 4.8. In addition, the amount of tap hot water required per year was determined as 17 m3 per year. Cold water at 5 °C had to be heated until 55 °C to obtain the tap hot water.Once the heating requirements were known, the most suitable solution was to use a combi system (solar thermal collectors and a heat pump). Solar energy could fulfil the demand in the summer and the heat pump provided energy in the winter. For a commercial model of the flat solar thermal collector (Vitosol 100-F) the solar system was sized according to the heating demand in the summer time. The maximum energy that could be obtained from the solar collectors in summer was calculated, the rest of the demand had to be fulfilled by a heat pump, model WPL-18 E.The achieved solution is compounded by the heat pump and 3 solar thermal collectors with a surface of 2.33 m2 each. The solar energy obtained is 1 843 kWh per year, which covers 9 % of the total annual heating demand (20 098 kWh). However, the 98 % of the heating demand during the summertime comes from the solar collectors. The investment cost is 113 900 SEK and the payback period is estimated in 8 years.

Na produção de suco de uva, a adição de preparados enzimáticos durante a maceração, antes da prensagem, é um pré-requisito para a obtenção de rendimentos satisfatórios de suco, além de melhorar a qualidade organoléptica, promovendo uma maior extração de cor e compostos bioativos. Uma segunda aplicação enzimática pode ser realizada após o processo de prensagem no suco ainda turvo, buscando reduzir essa turbidez, o que facilitará os processos de clarificação e filtração posteriores, aumentando a produtividade dessas etapas. Porém, existe um grande número de enzimas comerciais disponíveis para a aplicação na indústria de sucos, sendo assim, é importante avaliar o potencial de cada preparado enzimático para uma utilização correta. Desta forma, o objetivo deste trabalho foi avaliar a influência da composição dos diferentes preparados enzimáticos na extração, qualidade e compostos bioativos do suco de uva elaborado com a variedade Concord. Primeiramente, o efeito de oito diferentes preparados enzimáticos foi avaliado para extração e qualidade do suco de uva. Subsequentemente, os dois preparados enzimáticos que se destacaram (Pectinex® Ultra Clear e Lallzyme® Beta), foram utilizados de forma sinérgica em um planejamento experimental, buscando encontrar a condição ótima de tempo, temperatura, concentração de enzima e proporção de Pectinex® Ultra Clear/Lallzyme® Beta, que pudesse maximizar a extração do suco de uva. E, por fim, foram obtidos agregados enzimáticos entrecruzados (CLEAs), como forma de imobilização das diferentes enzimas encontradas nos preparados enzimáticos para a clarificação do suco de uva. Os experimentos foram realizados no Laboratório de Biocatálise e Tecnologia Enzimática do ICTA/UFRGS. As uvas utilizadas nos experimentos foram da variedade Concord fornecida pela empresa Vitivinícola Jolimont, de Canela, RS. As preparações enzimáticas utilizadas foram caracterizadas em relação a cinco atividades enzimáticas, sendo elas, pectinase total (PE), poligalacturonase (PG), pectina liase (PL), pectina metil esterase (PME) e celulase (CE). Desta mesma forma, os combi-CLEAs foram caracterizados em relação as suas atividades enzimáticas, além disso, sua estabilidade, reutilização e pH e temperatura ótima foram avaliados. Os compostos bioativos foram identificados e quantificados por HPLC-DAD-MS (cromatografia líquida de alta eficiência - detector de arranjo de diodos - espectro de massa) e a atividade antioxidante foi avaliada através dos métodos de ABTS e de capacidade redutora. Rendimentos superiores de suco foram obtidos com os preparados que apresentaram altas concentrações de PG e PME ou PL. Quando, o preparado Pectinex® Ultra Clear (PUC) foi utilizado, observou-se um aumento de 8,7% na quantidade de suco extraído em comparação com o controle. Já os maiores valores de compostos bioativos foram encontrados com os preparados que apresentaram em sua composição uma maior atividade CE (Lallzyme® Beta, LB). Entretanto, quando PUC e LB foram adicionados em conjunto, maiores valores de rendimento e compostos bioativos foram encontrados. Já os combi-CLEAs, se mostraram como uma ótima forma de enzima imobilizada para a clarificação do suco de uva. Dentre os combi-CLEAs produzidos, destacaram-se o combi-CLEA-BSA devido à melhor atividade enzimática e maior estabilidade térmica (3 vezes mais que a enzima solúvel), podendo ser reutilizados por 6 ciclos com conversão total do substrato em produto. Além disso, o combi-CLEA-BSA melhorou a clarificação do suco, obtendo uma redução da turbidez de 56.7 % em 1 h. Por fim, pode-se afirmar que o trabalho atingiu melhorias produtivas e qualitativas ao suco de uva através da tecnologia enzimática. / In grape juice production, the addition of enzyme preparations during the maceration prior to pressing is a prerequisite for obtaining satisfactory juice yield, besides it improves the organoleptic quality, improving color and bioactive compounds extraction. A second enzyme application may be performed after the pressing process in cloudy juice, in order to reduce this turbidity, facilitating clarification and filtration processes. However, there are a great number of commercial enzymes available to the application on the fruit juice industry, so it is important to know the ability of each enzyme preparation for correct use. Thus, the aim of this study was to evaluate the influence of the composition of the different enzymatic preparations in the extraction, quality and bioactive compounds of grape juice from Concord variety. Firstly, the effect of eight different enzyme preparations was evaluated for extraction and quality of grape juice. Subsequently, the best enzyme preparations (Pectinex® Ultra Clear and Lallzyme® Beta) were used in an experimental design to find the optimal time, temperature, enzyme concentration and ratio of Pectinex® Ultra Clear/Lallzyme® Beta which could maximize the extraction of grape juice. Finally, cross-linked enzyme aggregates (CLEAs) were obtained as a way to immobilize the different enzymes found in the enzyme preparations for grape juice clarification. The experiments were performed at the Laboratory of Biocatalysis and Enzyme Technology, ICTA/UFRGS. The grapes (Concord variety) were provided by Vitivinícola Jolimont, Canela, RS. The enzymatic preparations were characterized through of five enzymatic activities, being total pectinase (PE), polygalacturonase (PG), pectinlyase (PL), pectin methyl esterase (PME) and cellulase (CE). In the same way, the combi-CLEAs were characterized regarding their enzymatic activities, thermal, pH and operational stabilities, and optimal pH and temperature. The bioactive compounds were identified and quantified by HPLC-DAD-MS (High Performance Liquid Chromatography - Diode Array Detector – Mass Spectrum) and the antioxidant activity was performed by the method of ABTS and reducing capacity. High juice yield was obtained with the preparations that presented higher concentrations of PG, PME or PL activities. The Pectinex® Ultra Clear, when compared with the control, provided an increase of 8.7 % in the amount of juice extracted. On the other hand, the highest values of bioactive compounds were found for preparations with higher CE activity (Lallzyme® Beta). However, when PUC and LB were added together, higher values for yield and bioactive compounds were found. Already, regarding the combi-CLEAs, it appears to be a potential immobilized enzyme for clarification of grape juice. Among the combi-CLEAs obtained, combi-CLEAs-BSA presented the best enzyme activities, higher thermal stabilities (3 times more than the soluble enzyme) and it can be reused for 6 cycles with a total conversion of substrate to product. Furthermore, the combi-CLEA-BSA improved the juice clarification, obtaining 56.7 % in turbidity reduction in 1 h. Finally, it can be concluded that the work reached productive and qualitative improvements for grape juice through enzymatic technology.