Global ETD Search

1	DISTINCT ROLES OF THE aD HELIX IN aCAMKII ACTIVATION CHARACTERIZED USING A DE NOVO MUTATION FROM CHILDREN WITH LEARNING DISABILITIES Walter Saide (16650807) 07 August 2023 (has links) <p>This dissertation describes the effects of a <i>de novo</i> mutation of CaMKII found in children with learning disabilities and describes its effect on catalytic activity. We develop a malachite green assay for the measurement of CaMKII activation and use it for high-throughput chemical screening to identify CaMKII inhibitors and enhancers. We also propose a new mechanism of regulation of CaMKII activity by ADP.</p><p><br></p> Cell neurochemistry Enzymes Basic pharmacology Biologically active molecules Biomolecular modelling and design Proteins and peptides Catalysis and mechanisms of reactions Reaction kinetics and dynamics CaMKII α learning and memory impairment calmodulin calcium signaling kinase activity medicinal chemistry molecular pharmacology enzyme kinetics western blotting biochemistry molecular modeling high throughput chemical screening assay development protein structure enzyme coupled assays malachite green assay cellular biology fluorescence microscopy hplc lc-ms/ms autophosphorylation ATP:ADP ratios