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Avaliação da neoformação óssea em tíbia de coelhos utilizando cúpula de hidroxiapatita associada a diferentes biomateriais / Evaluation of new bone formation in rabbits using hydroxyapatite dome associated to different materialsMAEDA, NANCY T. 09 October 2014 (has links)
Made available in DSpace on 2014-10-09T12:41:41Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:07:15Z (GMT). No. of bitstreams: 0 / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Avaliação da neoformação óssea em tíbia de coelhos utilizando cúpula de hidroxiapatita associada a diferentes biomateriais / Evaluation of new bone formation in rabbits using hydroxyapatite dome associated to different materialsMAEDA, NANCY T. 09 October 2014 (has links)
Made available in DSpace on 2014-10-09T12:41:41Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:07:15Z (GMT). No. of bitstreams: 0 / A instalação de implantes odontológicos requer a presença de substrato ósseo adequado para garantir estabilidade e equilíbrio biomecânico. A deficiência óssea requer procedimentos de enxertia para adequar o volume para a instalação de implantes, porém a utilização de enxertos autógenos causa aumento de morbidade ao paciente e o uso de material homógeno e xenógeno apresenta dúvidas quanto à reação autoimune, transmissão de doenças e ao grau de reabsorção do enxerto. Com o grande desenvolvimento científico e tecnológico dos biomateriais, os materiais cerâmicos, tornaram-se alternativas promissoras para a recomposição da estrutura óssea perdida. As cerâmicas à base de fosfato de cálcio como a hidroxiapatita (HA) e o beta- fosfato tricálcido (beta-TCP), são materiais que apresentam qualidades desejáveis no processo de neoformação óssea como, por exemplo, a biocompatibilidade, bioatividade e osteocondutividade. A proposta deste trabalho é desenvolver e estudar corpos de prova na forma de cúpula oca de hidroxiapatita preenchidos por coágulo, beta- TCP e composto vitamínico, para estudar a osteogênese supracortical, a partir do potencial osteocondutor da cúpula de HA. As cúpulas foram obtidas por prensagem isostática a 200 MPa e sinterização ao ar a 1100°C por 60 minutos. As caracterizações físico-químicas das matérias-primas e da cúpula de HA foram realizadas por difração de raios X, microscopia eletrônica de varredura e determinação da densidade. Na caracterização biológica, foram realizados o teste de citotoxicidade in vitro e ensaio in vivo. Foram designados 9 coelhos (raça Nova Zelândia), sendo instaladas 18 cúpulas, divididas em três grupos, de acordo com o preenchimento: controle, composto vitamínico e β-TCP em forma de pó. O período de reparação tecidual foi de 8 semanas, no qual foram aplicados marcadores de fluorescência. Após o período de cicatrização e eutanásia, as amostras foram incluídas em resina para a obtenção das lâminas e observadas em microscópio de fluorescência, para avaliar a quantidade de tecido ósseo neoformado, em microscópio de campo claro, para verificar as células presentes no tecido formado e por Espectroscopia de Energia Dispersiva, para análise química, da formação no interior das cúpulas. Como resultados, a cúpula de hidroxiapatita apresenta bom desempenho como arcabouço para neoformação óssea acima da cortical da tíbia de coelhos, pois manteve-se íntegra, com boa estabilidade e boa integração ao tecido ósseo, e principalmente pela neoformação óssea, demonstrando seu potencial osteocondutor. Em relação aos materiais de preenchimento, o beta-TCP apresenta maior valor de área de osso neoformado, em comparação com o coágulo. Nas cúpulas com preenchimento de composto vitamínico, não há formação de tecido ósseo pela não reabsorção do material. / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP
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Céramiques phosphocalciques fonctionnalisées : étude des propriétés de surface par méthodes spectroscopiques / Functionalised phosphocalcic ceramics : study of surface properties by spectroscopic methodsEl Felss, Nadia 14 December 2018 (has links)
Ce travail s’inscrit dans le cadre général du développement de biomatériaux ostéoinducteurs pour la réparation de grands défauts osseux. L’étude est une contribution à la compréhension des interactions physiques et chimiques entre des céramiques phosphocalciques et deux protéines d’intérêt : la fibronectine, protéine d’adhésion cellulaire, et le VEGF (pour Vascular Endothelial Growth factor) qui est impliqué dans la vascularisation et l’amélioration de la formation osseuse.Les interactions physiques fibronectine/biocéramique ont été étudiées par spectroscopie de force afin d’évaluer l’influence de la topographie et de la composition chimique de céramiques phosphocalciques en hydroxyapatite (HA), hydroxyapatite silicatée (SiHA) et hydroxyapatite carbonatée (CHA) sur l’adhésion de la fibronectine. Les résultats obtenus par cartographie de forces mettent en évidence une absence d’incidence de la chimie des céramiques polies sur la répartition en surface et l’intensité des forces d’adhésion. En revanche ces dernières sont plus fortes au niveau des joints de grains des céramiques non polies mettant en avant une influence de la topographie de surface des matériaux modulée par la chimie.Le protocole de fonctionnalisation par le VEGF consiste en trois étapes : silanisation, addition du SM(PEG)6 et immobilisation du VEGF. Les interactions chimiques VEGF/biocéramique ont été étudiées principalement par imagerie Raman pour suivre ces étapes successives de la fonctionnalisation par le VEGF de céramiques polies en hydroxyapatite (HA) et hydroxyapatite carbonatée (CHA). Cette approche a permis de cartographier l’évolution chimique de la surface des matériaux et de mettre en évidence la distribution spatiale ainsi que les réactions préférentielles entre les molécules intermédiaires et le VEGF en fonction de la nature du substrat. / This work is ascribed within the framework of the development of osteoinductive biomaterials for the repair large bone defects. It is a contribution to the understanding of the physical and chemical interactions between phosphocalcic ceramics and two proteins of interest: fibronectin (Fn), a cell adhesion protein, and Vascular Endothelial Growth Factor (VEGF) which is involved in vascularisation and improvement of bone formation.Fibronectin/bioceramic physical interactions were studied by force spectroscopy to evaluate the influence of the topography and the chemical composition of phosphocalcic ceramics made of hydroxyapatite (HA), silicated hydroxyapatite (SiHA) and carbonated hydroxyapatite (CHA) on fibronectin adhesion. The results obtained in terms of force cartography do not indicate any impact of the polished ceramics chemistry on the surface distribution and intensity of adhesion forces. However, these forces are more intense at the level of the grain boundaries of unpolished ceramics, highlighting an influence of the topography modulated by the chemical composition.The protocol for functionalisation by VEGF consists of three steps: silanisation, addition of SM(PEG)6 and immobilisation of VEGF. VEGF/bioceramic chemical interactions were studied mainly by Raman imaging in order to follow the successive steps of the functionalisation by VEGF of the polished surface of ceramics made of hydroxyapatite (HA) and carbonated hydroxyapatite (CHA). This approach allowed to map the surface chemical changes and to point out the spatial distribution as well as the preferential reactions between the intermediate molecules and VEGF depending of the substrate.
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An In-Vitro Comparison of Microleakage With E. faecalis In Teeth With Root-End Fillings of Proroot MTA and Brasseler's EndoSequence Root Repair PuttyBrasseale, Beau J. (Beau John), 1980- January 2011 (has links)
Indiana University-Purdue University Indianapolis (IUPUI) / Brasseler USA (Savannah, GA) developed and introduced a bioceramic putty called EndoSequence Root Repair Material (ERRM) that can be used as a retrofilling material for surgical endodontics. The material is said to have many of the same chemical, physical, and biological properties as mineral trioxide aggregate (MTA), but with superior handling characteristics. The material is composed of calcium silicates, monobasic calcium phosphate, zirconium oxide, tantalum oxide, proprietary fillers, and thickening agents. ERRM is said by the manufacturer to bond to adjacent dentin, have no shrinkage, be highly biocompatible, hydrophilic, radiopaque, and antibacterial due to a high pH during setting. Investigations on the sealing properties of this material have not yet been conducted.
The purpose of this study was to compare the microbial leakage of Enterococcus faecalis in teeth with root-end fillings using ProRoot MTA and Brasseler’s ERRM in a dual-chamber bacterial leakage model as described by Torabinejad and colleagues. The aim of this investigation was to compare the bacterial microleakage of these two root-end filling materials exists.
Sixty-two human, single-rooted, mandibular premolars in which extraction was indicated were accessed and instrumented in an orthograde fashion with hand and rotary files. Root resection of the apical 3 mm was then completed and root-end retropreparations were created for placement of root-end filling material. Twenty-seven of these premolars had root-end fillings using ProRoot MTA and 27 had root-end fillings using ERRM. Two teeth were used as a positive control group with no root-end filling, and two other teeth were used as a negative control group and were sealed and coated with dentin bonding agent. The teeth were then evaluated for microleakage using a dual-chamber bacterial microleakage model for 40 days as described by Torabinejad and colleagues. Microleakage was determined by the presence of turbidity in the lower chamber of the apparatus and was assessed each day. Fresh samples of E. faecalis were used every three days to inoculate the apparatus and serve as a bacterial challenge for the materials. Results were recorded every day for 30 days. The outcome of interest (bacterial turbidity) and time-to-leakage (in days) were determined for each of the samples. Survival analysis was used to compare the two groups with a Kaplan-Meier plot to visualize the results and a nonparametric log-rank test for the group comparison.
The microleakage of ERRM was not statistically different (p > 0.05) than leakage of ProRoot MTA when subjected to E. faecalis over the 40 day observation period. Both groups had a small number of early failures (within 4 days) and no leakage was observed for the remaining 40 days of the study. Therefore, the null hypothesis was rejected.
The results of this research support the use of either of these two materials when compared with the controls. The microleakage of Brasseler’s EndoSequence Root Repair Material was at least as good as ProRoot Mineral Trioxide Aggregate when tested with E. faecalis.
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