Thesis (Ph. D.)--University of Wisconsin--Madison, 1955. / Typescript. Vita. eContent provider-neutral record in process. Description based on print version record. Includes bibliographical references (leaves 43-46).

Thesis (M.S.)--Virginia Polytechnic Institute and State University, 1993. / Vita. Also available via the Internet.

Thesis (Ph. D.)--University of North Texas, May, 2009. / Title from title page display. Includes bibliographical references.

This epidemiological investigation focussed on <i>Campylobacter jejuni </i>and <i>Campylobacter coli</i> in the north east of Scotland (Grampian). Scotland, in 2007, had an annual incidence of 101.5 cases/100,000 people, while in Grampian this figure was 120/100,000.  <i>C. jejuni </i>and <i>C. coli </i>are ubiquitous and are natural commensals of a range of animals, therefore understanding the epidemiology of these organisms involves identifying their putative sources and monitoring their dynamics in such hosts.  In this study, the use of various epidemiological tools showed that humans are infected from different sources and notably by chicken. Between 2001 and 2006, the population structure of <i>Campylobacter </i>in retail chicken changed  more than in humans, as shown by characterisation by Multilocus sequence typing (MLST).  There was however a high degree of overlap between these respective strains suggesting that chickens are a major source of human infection.  Furthermore, comparison of the same strains using antimicrobial resistance profiles revealed the same commonality of strains. Infection in the high risk 0-4 year old age group was shown to be attributable mainly to chicken in an urban environment but more attributable to cattle in rural areas.  This may reflect relative rates of exposure to these important sources of <i>Campylobacter.</i> Characterisation of a large outbreak in north east Scotland using various typing techniques identified chicken liver paté as a vehicle for <i>C. jejuni </i>infection; multiple strains were identified in the food and more than one patient displayed co-infection with different strains. These investigations have provided useful information which contributes to the overall surveillance of human campylobacteriosis and the epidemiology of <i>Campylobacter </i>in different hosts.

Thesis (MSc. (Veterinary Science))--University of Pretoria, 2005. / Includes bibliographical references.

Campylobacter organisms are the most commonly reported bacterial causes of foodborne infection in the world, with Campylobacter jejuni and Campylobacter coli responsible for over 99% of reported infections. Traditionally, Campylobacter species detection is an arduous process, requiring a special incubation environment as well as specific growth media for an extended growth period. The development of a rapid and reliable diagnostic tool for the detection of Campylobacter species would be a valuable aid to the medical diagnostic decision process, especially to rule out Campylobacter infection during the enteric pre-surgical time period. Improved patient outcomes would result if this rapid assay could reduce the number of enteric surgeries. Assays performed during this dissertation project have demonstrated that both SYBR® green and hydrolysis probe assays targeting an 84 nucleotide portion of cadF, a fibronectin-binding gene of Campylobacter jejuni and Campylobacter coli, were able to detect from 101 to 108 copies of organism from stool specimens, did not detect nonspecific targets, and exhibited a coefficient of variation (CV) of 1.1% or less. Analytical validation of sensitivity, specificity and precision, successfully performed in these studies, warrants additional clinical validation of these assays.

Thesis (M.S.)--West Virginia University, 2001. / Title from document title page. Document formatted into pages; contains viii, 90 p. : ill. (some col.). Vita. Includes abstract. Includes bibliographical references (p. 59-66).

Thesis (M. Sc.)--University of Canterbury, 2007. / Typescript (photocopy). Includes bibliographical references (leaves 124-142). Also available via the World Wide Web.

Thesis--Auburn University, 2008. / Abstract. Vita. Includes bibliographical references (p. 88-101).

QUETZ, Josiane da Silva. Estudo sobre Campylobacter jejuni e Campylobacter coli em crianças da área urbana de Fortaleza, Ceará/Brasil : identificação genética, inflamação intestinal e impacto no estado nutricional. 2009. 142 f. Dissertação (Mestrado em Farmacologia) - Universidade Federal do Ceará. Faculdade de Medicina, Fortaleza, 2009. / Submitted by denise santos (denise.santos@ufc.br) on 2012-04-12T12:44:16Z No. of bitstreams: 1 2009_dis_jsquetz.pdf: 1671276 bytes, checksum: 7b86044a2d433a11e1abdc0273d371d4 (MD5) / Approved for entry into archive by Eliene Nascimento(elienegvn@hotmail.com) on 2012-04-13T16:16:02Z (GMT) No. of bitstreams: 1 2009_dis_jsquetz.pdf: 1671276 bytes, checksum: 7b86044a2d433a11e1abdc0273d371d4 (MD5) / Made available in DSpace on 2012-04-13T16:16:02Z (GMT). No. of bitstreams: 1 2009_dis_jsquetz.pdf: 1671276 bytes, checksum: 7b86044a2d433a11e1abdc0273d371d4 (MD5) Previous issue date: 2009 / Campylobacter jejuni and Campylobacter coli are important etiologic agents of worldwide diarrheal disease. Campylobacter sp. infection is usually identified by a 72 hour microbiological culture that identifies the genus of the responsible organism. Our main goal was to investigate the prevalence of C. jejuni and C. coli in children, aged 2-36 months, from urban Fortaleza, CE, Brazil, in an observational epidemiological case-control study using, as a tool of detection, the polymerase chain reaction (PCR). Our other goals were to investigate the nutritional impact of infection (cases) or colonization (controls) for Campylobacter sp., to determine the presence of three virulence genes of C. jejuni cytolethal distending toxin (CDT), and to evaluate the occurrence of inflammation in intestinal infections caused by Campylobacter sp. The study population consisted of 83 cases and 83 controls, where the cases consisted of children with a history of diarrhea in the 14 days prior to selection for the study. We assessed socioeconomic parameters through an epidemiological questionnaire. Anthropometric measurements were collected to determine z-score parameters for assessing the nutritional status of the children. Detection of Campylobacter from frozen samples was performed by enzyme-linked immunosorbent assay (ELISA) and PCR. Also, using PCR technology, we investigated the presence of C. jejuni genes cdtA, cdtB and cdtC. Intestinal inflammation was assessed by semi-quantitative ELISA detection of fecal lactoferrin (LFF). PCR technology detected C. jejuni in 9.6% of the cases (8/83) and 7.2% of the controls (6/83), while C. coli was detected in 6.0% of the cases (5/83) and 1.2% of the controls (1/83). CDT genes were found in 50% of hipO+ samples (7/14). There was a significant difference (p <0.05) in the weight for age z-scores (WAZ) and the weight for height z-scores (WHZ) between case and control carriers of C. jejuni, where case carriers showed lower average WAZ and WHZ than control carriers. Moreover, in the case group, carriers of C. jejuni showed a lower WHZ average than that of non-carrier cases of C. jejuni. More than 80.0% of the children studied had intestinal inflammation characterized by high levels of LFF regardless of the presence of diarrhea and Campylobacter sp. In conclusion, our findings corroborate data in the scientific literature related to the prevalence of C. jejuni and C. coli in pediatric populations, the existence of asymptomatic carriers and an association between the detection of the microorganism and malnutrition. In addition, our data suggest a genetic variability among the strains of C. jejuni detected in the study population, related to presence o absence of CDT genes. / Campylobacter jejuni e Campylobacter coli são importantes agentes etiológicos de doença diarréica na população mundial. A infecção por Campylobacter sp. é usualmente identificada por cultivo microbiológico que leva aproximadamente 72 horas para identificação do gênero. Nosso objetivo principal foi pesquisar a prevalência de C. jejuni e C. coli em população infantil, com idade entre 2-36 meses, da área urbana de Fortaleza/CE, Brasil, em estudo do tipo epidemiológico observacional caso-controle, utilizando, como ferramenta de detecção, a reação em cadeia da polimerase (PCR). Outros objetivos consistiram em: investigar o impacto nutricional da infecção (casos) ou da colonização (controles) por Campylobacter sp.; determinar a presença de três genes de virulência para a toxina citoletal distensora (CDT) de C. jejuni e avaliar a ocorrência de inflamação intestinal nas infecções causadas por Campylobacter sp. A população estudada consistiu de 83 casos e 83 controles, sendo os casos, crianças com histórico de diarréia nos 14 dias pregressos à seleção para o estudo. Foram avaliados parâmetros sócio-econômicos através de questionário epidemiológico. Medidas antropométricas foram coletadas para determinação de escores-z no intuito de avaliar o perfil nutricional das crianças. A detecção de Campylobacter nas amostras congeladas foi realizada por ensaio imuno-enzimático (ELISA) e PCR. Pela PCR também investigamos a presença dos genes cdtA, cdtB e cdtC da CDT de C. jejuni. A avaliação da inflamação intestinal foi realizada pela pesquisa de lactoferrina fecal (LFF), através de ELISA semiquantitativa. Foi detectado, por PCR, C. jejuni em 9,6% dos casos (8/83) e 7,2% dos controles (6/83). C. coli foi detectado em 6,0% dos casos (5/83) e 1,2% dos controles (1/83). Os genes cdtA, cdtB e cdtC foram encontrados em 50% das amostras hipO+ (7/14). Houve diferença significativa (p<0,05) dos escores WAZ e WHZ entre casos e controles portadores de C. jejuni, sendo que casos portadores apresentaram média inferior de WAZ e WHZ, quando comparados com os controles portadores. No grupo Casos, os portadores de C. jejuni apresentavam valor médio de WHZ inferior ao valor médio apresentado pelos casos não-portadores. Mais de 80,0% das crianças estudadas apresentaram inflamação intestinal caracterizada por elevados níveis de LFF, independente da presença de diarréia e Campylobacter sp. Em conclusão, nossos achados corroboram dados da literatura científica relacionados à prevalência de C. jejuni e C. coli na população infantil, existência de portadores assintomáticos e associação entre a detecção do microorganismo e desnutrição. Além disso, nossos dados apontam para ocorrência de variabilidade genética dentre as cepas de C. jejuni detectadas na população estudada em relação à presença ou ausência dos genes de CDT.