Orientador: Maria Denise Lopes / Banca: Frederico Ozanan Papa / Banca: Jussara Tebet / Resumo: A análise dos efeitos da refrigeração sobre a congelabilidade da célula espermática da cauda do epidídimo de gatos domésticos foram avaliados nesse experimento. Após prévia refrigeração a 5O C por 24 horas alíquotas de sêmen foram submetidas a criopreservação e posteriormente descongeladas para análise morfofuncional. 15 animais foram submetidos a orquiectomia de conveniência e seus epidídimos foram manipulados para obtenção de amostras que foram suspensas em meio TE (TRIS/Equex/Gema de Ovo), analisadas, refrigeradas e congeladas. Amostras referentes ao grupo controle foram congeladas imediatamente após a colheita. A analise morfofuncional consistiu em motilidade, vigor, morfologia espermática e Integridade de membrana. Após a descongelação os resultados dos grupos controle e trabalho foram analisados estatisticamente e observou-se que não existe diferença estatística para nenhuma das variáveis analisadas. Embora tenha sido observada diferença estatística entre os momentos pós-colheita e pós-refrigeração por 24 horas para as variáveis motilidade, vigor, integridade de membrana e defeitos primários, nenhuma variável diferiu entre as amostras congeladas pós-colheita e as amostras congeladas pós-refrigeração prolongada. Tais resultados demonstram que um transporte refrigerado a 5O C por 24 horas não interfere sobre a congelabilidade de sêmen do epidídimo de gatos domésticos. Do ponto de vista da conservação tais resultados abrem portas para um melhor fluxo de gametas de espécies selvagens para centrais de biotecnologia permitindo um melhor aproveitamento e conservação deste material. / Abstract: The present study analyzed the effect of cooling on sperm cells obtained from the epididymis tail from domestic cats in respect to their capability for freezing. The treatment group had their samples submitted to freezing after being cooled at 5° C during 24 hours, and th~ control group samples were frozen immediately after collection, Samples from both groups were then thawed and submitted to mo rphofunctiona I analysis. Fifteen animais were submitted to a convenient routine orchiectomy and their epididymis were processed to obtain a semen samples which were suspended in TE medium (TRIS/Equexl egg yolk). The samples were then analyzed, coo,led and frozen. The morphofunctional analysis consisted of sperm motility, vigor, morphology and membrane integrity. After thawing, the semen samples from treatment and control groups were analyzed and no statistic difference was found among ali variables described above. / Mestre

Made available in DSpace on 2014-06-11T19:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2006-07-29Bitstream added on 2014-06-13T19:17:43Z : No. of bitstreams: 1 martins_jla_me_botfmvz.pdf: 366556 bytes, checksum: 5564f8a57d5ae434024a1843feeb124b (MD5) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A análise dos efeitos da refrigeração sobre a congelabilidade da célula espermática da cauda do epidídimo de gatos domésticos foram avaliados nesse experimento. Após prévia refrigeração a 5O C por 24 horas alíquotas de sêmen foram submetidas a criopreservação e posteriormente descongeladas para análise morfofuncional. 15 animais foram submetidos a orquiectomia de conveniência e seus epidídimos foram manipulados para obtenção de amostras que foram suspensas em meio TE (TRIS/Equex/Gema de Ovo), analisadas, refrigeradas e congeladas. Amostras referentes ao grupo controle foram congeladas imediatamente após a colheita. A analise morfofuncional consistiu em motilidade, vigor, morfologia espermática e Integridade de membrana. Após a descongelação os resultados dos grupos controle e trabalho foram analisados estatisticamente e observou-se que não existe diferença estatística para nenhuma das variáveis analisadas. Embora tenha sido observada diferença estatística entre os momentos pós-colheita e pós-refrigeração por 24 horas para as variáveis motilidade, vigor, integridade de membrana e defeitos primários, nenhuma variável diferiu entre as amostras congeladas pós-colheita e as amostras congeladas pós-refrigeração prolongada. Tais resultados demonstram que um transporte refrigerado a 5O C por 24 horas não interfere sobre a congelabilidade de sêmen do epidídimo de gatos domésticos. Do ponto de vista da conservação tais resultados abrem portas para um melhor fluxo de gametas de espécies selvagens para centrais de biotecnologia permitindo um melhor aproveitamento e conservação deste material. / The present study analyzed the effect of cooling on sperm cells obtained from the epididymis tail from domestic cats in respect to their capability for freezing. The treatment group had their samples submitted to freezing after being cooled at 5° C during 24 hours, and th~ control group samples were frozen immediately after collection, Samples from both groups were then thawed and submitted to mo rphofunctiona I analysis. Fifteen animais were submitted to a convenient routine orchiectomy and their epididymis were processed to obtain a semen samples which were suspended in TE medium (TRIS/Equexl egg yolk). The samples were then analyzed, coo,led and frozen. The morphofunctional analysis consisted of sperm motility, vigor, morphology and membrane integrity. After thawing, the semen samples from treatment and control groups were analyzed and no statistic difference was found among ali variables described above.

The resident feral cat (Felis catus) population on the University of KwaZulu-Natal (UKZN), Howard College campus (HCC) in Durban, South Africa was studied from March 2004 to November 2005. This study was initiated as the HCC is an registered as an urban conservancy and so should be removing alien invasive flora and fauna and conserving the indigenous biodiversity of the campus. This research was undertaken to assist with recommendations for the control and management of feral cats on the HCe. A survey to determine public perceptions and opinions regarding the feral cats was conducted among various communities on the campus. Feral cats from the resident population on the HCC were trapped and fitted with radio-collars in order that their home range sizes and distribution could be determined. Monthly census counts were also carried out in an attempt to calculate population densities of the feral cats on campus, while data on behaviour patterns was collected opportunistically throughout the study period. The survey showed that two extreme views existed on campus regarding the presence of feral cats. The university is a registered conservancy which some feel is no place for this exotic species. However, it is also situated within an urban surrounding and there are some cat enthusiasts among the public who feel that resources should be provided for the feral cats, both nutritionally and financially. While many people were unaware that the feral cats were a cause for concern on the HCC, the majority concluded that a management policy needed to be adopted to control feral cat numbers. Most were against the suggestion of eradicating the cats and strongly agreed with the implementation of a university funded feral cat IV sterilising and feeding programme. Feeding the feral cats, however, needs to be stringently controlled. In this study, the availability of an abundance of food resources was shown to be the primary influencing factor for home range size, cat distribution and population densities. It also had an overriding effect on the feral cats' behaviour patterns and activity levels. Once these had been initially established, other factors such as human activity, reproductive status and gender then came into play. Distribution of the feral cats around campus was not homogenous, and densities differed according to areas on campus. Highest cat densities were recorded in those areas on the HCC where permanent cat feeding stations had been established (usually the developed areas on campus), while no feral cats were sighted in the Msinsi Nature Reserve, a natural bush area on campus were no food resources (other than prey species) is available. Home range sizes of the feral cats were relatively small with a considerable amount of overlap between and within the sexes. There were also no seasonal differences in range sizes and diurnal ranges were only marginally smaller than nocturnal range sIzes. In terms of behaviour, the HCC feral cats were generally inactive, with passive behaviour such as lying down and sitting being most often observed. Although hunting activity was very rarely witnessed, the combined effects of feral cats supported at high densities by supplemental feeding may exert predation pressures that could be detrimental to both local prey and predator populations. Little social interactions were observed by the cats on the HCC. Other studies show that competition is greatly reduced if food is available in abundance and there is no need for territorial disputes if both food and a potential mate are located in close proximity. In the present study, this also means that immigrating feral cats from surrounding neighbourhoods are tolerated; another factor contributing to the increase in feral cat numbers on the campus. These findings suggest that the feral cat population on the HCC is being maintained at higher population densities than would be expected and management initiatives are needed to control the feral cat population at a minimum density through a sterilising and low key feeding programme so that it is acceptable to all concerned parties. However, the decisions need to favour the status of the HCC as a conservancy in an urban area as well as consider the well-being of the students and staffmembers in a public place. / Thesis (M.Sc.)-University of KwaZulu-Natal, Pietermaritzburg, 2005.