In wireless communication systems, transmitted signals are corrupted by fading as well as noise. The receiver can benefit from the estimates of fading channels to detect the transmitted symbols. However, in practical wireless systems channel information cannot be estimated perfectly at the receiver. Therefore, it is crucial to examine the effect of channel estimation error on the structure and performance of the receivers. In the first part of the thesis, we study single-user systems with single-antenna reception over fading channels in the presence of Gaussian-distributed channel estimation error. By using the statistical information of the channel estimation error, we will derive the structure of maximum-likelihood receivers for a number of different modulation formats and then analyze their performance over fading channels. In the second part of the thesis, we consider the uplink of multi-user wireless systems with multi-antenna reception. For conventional diversity combining techniques such as maximal ratio combining and optimum combining we analyze the performance degradation due to imperfect channel estimates in the presence of multiple interfering users for several fading channels. By investigating the probability density function of the output signal-to-interference ratio, we will derive analytical expressions for several performance measures such as the average signal-to-interference ratio, outage probability and average bit-error probability. These expressions quantify performance degradation due to channel estimation error.

Achieving a higher transmission rate had always been a goal in the field of communications. Having a two-way channel in which two nodes transmit and receive data at the same time, is an important tool to achieve this goal. A two-way channel is the first step from point-to-point communication channel toward multi-user networks. In its ideal form, we can transmit data two times faster by using a perfect two-way channel. However, the area of two-way channels had not been of interest of researchers during the past years and number of articles on this area is considerably low comparing to other types of multi-user communication networks, such as multiple-access channel, broadcast channel and interference channel. On the other hand, use of analog-to-digital converters (ADC) is a must in modern systems to enable us to analyze data faster; nevertheless, presence of ADC add some other difficulties to the system. In this thesis, different scenarios about two-way channel are studied. The Shannon's model of two-way channel and his inner and outer bounds on the capacity of this channel are presented. For the Gaussian Two-Way Channel with quantized output, in which the ambient noise has a Gaussian distribution, the expression of Shannon's inner bound for both Gaussian and discrete inputs are derived. The best uniform quantizer to obtain the maximum achievable rate for Gaussian input is found numerically. Then we will evaluate the additive noise model for the quantizer from an information theoretic point of view. For the discrete input, the method of rotating one input with respect to other one is employed to enlarge the achievable rate region. At last, two scenarios will be studied in which, minimizing the power of interference, does not necessarily maximizes the transmission rate.

Orthogonal Frequency Division Multiplexing (OFDM) plays an important role in the implementation of high data rate communication. In this thesis, the problems of data detection and channel and carrier frequency offset estimation in OFDM systems are studied. <p>Multi-symbol non-coherent data detection is studied which performs data detection by processing multiple symbols without the knowledge of the channel impulse response (CIR). <p>For coherent data detection, the CIR needs to be estimated. Our objective in this thesis is to work on blind channel estimators which can extract the CIR using just one block of received OFDM data. A blind channel estimator for (Single Input Multi Output) SIMO OFDM systems is derived. The conditions under which the estimator is identifiable is studied and solutions to resolve the phase ambiguity of the proposed estimator are given.<p>A channel estimator for superimposed OFDM systems is proposed and its CRB is derived. The idea of simultaneous transmission of pilot and data symbols on each subcarrier, the so called superimposed technique, introduces the efficient use of bandwidth in OFDM context. Pilot symbols can be added to data symbols to enable CIR estimation without sacrificing the data rate. Despite the many advantages of OFDM, it suffers from sensitivity to carrier frequency offset (CFO). CFO destroys the orthogonality between the subcarriers. Thus, it is necessary for the receiver to estimate and compensate for the frequency offset. Several high accuracy estimators are derived. These include CFO estimators, as well as a joint iterative channel/CFO estimator/data detector for superimposed OFDM. The objective is to achieve CFO estimation with using just one OFDM block of received data and without the knowledge of CIR.

For ultra-wideband (UWB) communications, acquisition is challenging. The reason is from the ultra short pulse shape and ultra dense multipath interference. Ultra short pulse indicates the acquisition region is very narrow. Sampling is another challenge for UWB design due to the need for ultra high speed analog-to digital converter.<p>A sub-optimum and under-sampling scheme using pilot codes as transmitted reference is proposed here for acquisition. The sampling rate for the receiver is at the symbol rate. A new architecture, the reference aided matched filter is studied in this project. The reference aided matched filter method avoids using complex rake receiver to estimate channel parameters and high sampling rate for interpolation. A limited number of matched filters are used as a filter bank to search for the strongest path. Timing offset for acquisition is then estimated and passed to an advanced verification algorithm. For optimum performance of acquisition, the adaptive post detection integration is proposed to solve the problem from dense inter-symbol interference during the acquisition. A low-complex early-late gate tracking loop is one element of the adaptive post detection integration. This tracking scheme assists in improving acquisition accuracy. The proposed scheme is evaluated using Matlab Simulink simulations in term of mean acquisition time, system performance and false alarm. Simulation results show proposed algorithm is very effective in ultra dense multipath channels. This research proves reference aided acquisition with tracking loop is promising in UWB application.

This thesis summarizes the results of an experimental investigation involving the testing of push-out specimens with channel shear connectors. The test program involved the testing of 78 push-out specimens and was aimed at the development of new equations for channel shear connectors embedded in solid concrete slabs and slabs with wide ribbed metal deck oriented parallel to the beam. <p>The test specimens were designed to study the effect of a number of parameters on the shear capacity of channel shear connectors. Six series of push-out specimens were tested in two phases. The primary difference between the two phases was the height of the channel connector. Other test parameters included the compressive strength of concrete, the length and the web thickness of the channel. <p>Three different types of failure mechanisms were observed. In specimens with higher strength concrete, failure was caused by the fracture of the channel near the fillet with the channel web acting like a cantilever beam. Crushing-splitting of concrete was the observed mode of failure in specimens with solid slabs when lower strength concrete was used. In most of the specimens with metal deck slabs, a concrete shear plane type of failure was observed. In the specimens involving this type of failure, the channel connector remained intact and the concrete contained within the flute in front of channel web sheared off along the interface. <p>The load carrying capacity of a channel connector increased almost linearly with the increase in channel length. On average, the increase was about 39% when the channel length was increased from 50 mm to 100 mm. There was a further increase of 24% when the channel length was increased from 100 mm to 150 mm. The influence of web thickness of channel connector was significant when the failure occurred due to channel web fracture but was minimal for a concrete crushing-splitting type of failure. <p>The specimens with solid concrete slabs carried higher load compared to those with metal deck slabs. The increase in load capacity was 33% for specimens with 150 mm long channels but only 12% for those with 50 mm long channel connectors. <p>This investigation resulted in the development of a new equation for predicting the shear strength of channel connectors embedded in solid concrete slabs. The proposed equation provides much better correlation to test results than those obtained using the current CSA equation. <p>The results of specimens with metal deck slabs were used to develop a new equation for predicting the shear capacity of channel connectors embedded in slabs with metal deck oriented parallel to the beam. The values predicted by the proposed equation were in good agreement with the observed test values.

Cardiac ischemic preconditioning (IPC) is defined as brief periods of ischemia and reperfusion that protect the heart against longer ischemia and reperfusion. IPC triggers Cl- efflux and protein kinase C epsilon (PKCe) translocation to the particulate fraction. Chloride channel 2 (ClC-2) is volume regulated and is a potential end effector of IPC. The goal of my study was to investigate the involvement of PKCε and ClC-2 protein module in IPC of isolated adult rabbit ventricular myocytes. Co-immunoprecipitation (co-IP) assays on HEK 293 cells, transfected with ClC-2-Flag, confirmed that ClC-2 interacts with PKCe. Subcellular fractionation showed that PKCe/ClC-2 protein module is localized to the sarcolemma of cardiomyocytes. Lastly, ischemia/reperfusion injury was simulated in cardiomyocytes with 45min simulated ischemia (SI)/60min simulated reperfusion (SR) and IPC was induced by pre-treatment with 10min SI/20min SR. Co-IP after each time interval showed that IPC transiently enhanced PKCe/ClC-2 interaction. PKC inhibitor, GF109203X, abrogated the enhanced interaction.

Covert channels are becoming more popular as security risks grow in networks. One area that is promising for covert channels is wireless networks, since many use a collision avoidance scheme such as carrier sense multiple access with collision avoidance (CSMA/CA). These schemes often introduce randomness in the network, which provides good cover for a covert timing channel. In this thesis, we use the 802.11 standard as an example to demonstrate a wireless covert channel. In particular, most 802.11 configurations use a distributed coordinated function (DCF) to assist in communications. This DCF uses a random backoff to avoid collisions, which provides the cover for our covert channel. Our timing channel provides great improvements on other recent covert channels in the field of throughput, while maintaining high accuracy. We are able to achieve throughput over 8000 bps using Covert DCF, or by accepting a throughput of 1800 bps we can achieve higher covertness and 99% accuracy as well.

Potassium ion channels play a key role in the generation and propagation of action potentials. The S4-S5 linker peptide (L45) is believed to be responsible for the anesthetic/alcohol response of voltage-gated K+ channels. We investigated this region to define the structural basis of 1-alkanol binding site in dShaw2 K+ channel. L45 peptides derived from dShaw2 and hKv3.4 K+ channel, which, if part of the complete channel, demonstrate different sensitivity to 1-alcohols. Specifically, dShaw2 is alcohol sensitive and hKv3.4 is alcohol resistant. Structural analysis of L45 with NMR and CD suggested a direct correlation between alpha-helicity and the inhibition of dShaw2 channel by 1-butanol. We used CD and NMR to determine the structure of L45 peptides in micelles and vesicles. We measured spin-lattice relaxation time (T1) and determined the location and surface accessibility of L45 in micelles. These experiments confirm that L45 of dShaw2 adopts an α-helical conformation, partially buried in the membrane and parallel to the surface. The binding and accumulation of rev proteins to an internal loop of RRE (rev responsive element) of unspliced mRNA precursors is a key step of propagation of human immunodeficiency (HIV) virus. Molecules that interfere with this process can be expected to show anti-HIV activity. Our work is based on an assumption that zinc fingers could compete with rev proteins, therefore impeding the life cycle of HIV and stopping its infection. We studied the influence of different cations, anions, and the concentration of salts and osmolytes on the binding affinity with Polyacrylamide Gel Electrophoresis (PAGE) and Isothermal Titration Calorimetry (ITC). We conclude that the types of anions and/or cations and their concentrations affect the enthalpy and entropy of the binding interacitons. Using a gel assay, we confirm that there are three products in RNA-Protein reaction, and both EDTA and salts (and their concentrations) in the gel or samples interfere with RNA-protein complex mobility.

This thesis proposes a potential solution for future ubiquitous broadband wireless access networks, called a cellular wireless mesh network (CMESH), and investigates a number of its performance issues. A CMESH is organized in multi-radio, multi-channel, multi-rate and multi-hop radio cells. It can operate on abundant high radio frequencies, such as 5-50 GHz, and thus may satisfy the bandwidth requirements of future ubiquitous wireless applications.<p> Each CMESH cell has a single Internet-connected gateway and serves up to hundreds of mesh nodes within its coverage area. This thesis studies performance issues in a CMESH, focusing on cell capacity, expressed in terms of the max-min throughput. In addition to introducing the concept of a CMESH, this thesis makes the following contributions.<p> The first contribution is a new method for analyzing theoretical cell capacity. This new method is based on a new concept called Channel Transport Capacity (CTC), and derives new analytic expressions for capacity bounds for carrier-sense-based CMESH cells.<p> The second contribution is a new algorithm called the Maximum Channel Collision Time (MCCT) algorithm and an expression for the nominal capacity of CMESH cells. This thesis proves that the nominal cell capacity is achievable and is the exact cell capacity for small cells within the abstract models.<p> Finally, based on the MCCT algorithm, this thesis proposes a series of greedy algorithms for channel assignment and routing in CMESH cells. Simulation results show that these greedy algorithms can significantly improve the capacity of CMESH cells, compared with algorithms proposed by other researchers.

Due to its significant advantage in spectral efficiency, multiple-antenna communication technology will undoubtedly be a major component in future wireless system implementations. However, the full exploitation of this technology also requires perfect feedback of channel state information (CSI) to the transmitter-- something that is not practically feasible. This motivates the study of limited feedback systems, where CSI feedback is rate limited. This thesis focuses on the optimal design of limited feedback systems for three types of communication channels: the relay channel, the single-user point-to-point channel, and the multiuser broadcast channel. For the relay channel, we prove the efficiency of the Grassmannian codebooks as the source and relay beamforming codebooks, and propose a method for CSI exchange between the relay and the destination when global CSI is not available at destination. For the single-user point-to-point channel, we study the joint power control and beamforming problem and address the channel magnitude and direction quantization codebook design problem. It is shown that uniform quantization of the channel magnitude (in dB scale) is asymptotically optimal regardless of the channel distribution. The analysis further derives the optimal split of feedback bandwidth between the magnitude and direction quantization codebooks. For the multiuser broadcast channel, we first prove the sufficiency of a product magnitude-direction quantization codebook for managing the multiuser interference. We then derive the optimal split of feedback bandwidth across the users and their magnitude and direction codebooks. The optimization results reveal an inherent structural difference between the single-user and multiuser quantization codebooks: a multiuser codebook should have a finer direction quantization resolution as compared to a single-user codebook. It is further shown that the users expecting higher rates and requiring more reliable communication should provide a finer quantization of their CSI. Finally, we determine the minimum required total feedback rate based on users' quality-of-service constraints and derive the scaling of the system performance with the total feedback rate.