Cuticular hydrocarbons (CHCs) are found on the outer cuticle of all terrestrial arthropods. Although their primary function is in desiccation prevention, these compounds have also been shown to play a variety of roles in insect chemical communication, from species and sex recognition to providing cues of dominance and attractiveness. However, despite growing evidence of their versatility as cues, our knowledge of how CHCs are used in mating interactions is limited to Drosophila and field crickets. In this thesis I investigate the roles CHCs play in interactions at each stage of the mating process in the broad-horned flour beetle Gnatocerus cornutus. I assess the relative importance of CHCs in influencing male reproductive success and examine the complex interplay between different episodes of selection and the mechanisms of sexual selection acting on males. I use a combination of behavioural assays, experimental manipulations and gas chromatography. First, I identify the role of CHCs as cues of sperm competition risk and intensity, demonstrating how the presence of male-derived CHCs on the cuticles of virgin females elicits males to adjust their pre- and post-copulatory investment (chapter 2), by providing information on the state of their competitive environment. I then go on to look at the stability of CHCs as cues of sperm competition over time, finding that they are highly sensitive to environmental degradation (chapter 3) and do not persist in the habitat substrate of this species. Next, I investigate how male CHCs determine fighting and mating success. By estimating and comparing the strength and form of sexual selection imposed by male-male competition and female mate choice, I show that male CHCs are subject to strong antagonistic sexual selection (chapter 4). By experimentally manipulating male CHC profile, I then attempt to verify the selection gradients estimated for female choice 3 (chapter 5). However, my experimental manipulation fails to verify the importance of male CHCs for female mate choice. Finally, I explore the role of same-sex sexual behaviour (SSB) in determining male reproductive success (chapter 6). I find evidence to suggest that SSB may in fact be a form of aggression in its own right, and demonstrate that SSB and fighting may provide equivalent means for males to overcome female choice and secure a mating advantage. My results indicate that CHCs play key roles as chemical cues throughout the mating process and significantly impact male reproductive success. My thesis reveals the intricate nature of the relationships between mechanisms of sexual selection, alongside highlighting the need to consider both the social and physical environment when investigating the importance of chemical cues. I discuss the implications of these results for the evolution of male CHCs and how my findings can be used to further our knowledge of this field.

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Ephemeral patches of hydrodynamic and chemical sensory cues at fine scales are fundamentally important to the life success of plankton populations and thus the overall health and vitality of nearshore marine ecosystems. We employed various tools from experimental fluid mechanics to create ecologically-relevant hydrodynamic and chemical conditions in a recirculating flume system for zooplankton behavioral assays. The goal was to quantify and correlate changes in zooplankton behavior with coincident sensory cues. A laminar, planar free jet (the Bickley jet) was used to create finescale, free shear layers with targeted hydrodynamic characteristics as well as finescale, sharp-edged layers of both beneficial and toxic ("red tide") phytoplankton species. Planar particle image velocimetry (PIV) and laser-induced fluorescence (LIF) were used to quantify the flow and concentration fields, respectively. Behavioral assays with a variety of crustacean zooplankton species including Antarctic krill (Euphausia superba), estuarine crab larvae (Panopeus herbstii), and calanoid copepods (Temora longicornis and Acartia tonsa), each unique in its ecology, morphology, and life history, show clear and statistically-significant behavioral responses to relevant hydrodynamic and chemical cues. Estuarine crab larvae optimize short term and long term behavioral needs (foraging and habitat selection) by sensing and exploiting the information contained in multi-directional free shear flows. In the presence of thin layers of toxic algal exudates (Karenia brevis), T. longicornis and A. tonsa exhibit explicit avoidance behaviors through significant increases in swimming speed and overall behavioral variability resulting in a conspicuous hydrodynamic signature in a risk/benefit behavioral response. Finally, Antarctic krill exploit the hydrodynamic cues contained in a free shear layer to modify swimming behaviors and ultimately graze in a thin phytoplankton layer (Tetraselmis spp.). Each species is able to sense and exploit the information contained in coherent hydrodynamic and chemical sensory cues to change swimming kinematics and alter macroscale trajectory characteristics. Quantifying changes in zooplankton behavior in response to ecologically-relevant sensory cues is a crucial step towards modeling (e.g. via biophysically-coupled individual-based ecosystem models) and managing sustainable marine fisheries.

Orientador: José Roberto Trigo / Dissertação (mestrado) - Universidade Estadual de Campinas, Instituto de Biologia / Made available in DSpace on 2018-08-23T04:31:56Z (GMT). No. of bitstreams: 1 Mariscal_AdrianoAffonso_M.pdf: 5931496 bytes, checksum: beff506193372fed1f1ae62149a3f37d (MD5) Previous issue date: 2013 / Resumo: O resumo poderá ser visualizado no texto completo da tese digital / Abstract: The abstract is available with the full electronic document / Mestrado / Ecologia / Mestre em Biologia Animal

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Les rongeurs commensaux sont responsables de grands dommages en agriculture et dans les zones urbaines. En tant qu’espèces invasives, elles peuvent mettre en danger les espèces locales et sont porteurs et vecteurs de plusieurs zoonoses importantes. Les méthodes de contrôle sont basées principalement sur l’utilisation des warfarines, lesquelles produisent un grand nombre d’intoxications sur des espèces non ciblées et ont perdu une partie de leur efficacité à cause des résistances génétiques constatées chez les espèces cibles. De plus, ces méthodes sont considérées comme inhumaines parce qu’elles causent une mort lente et douloureuse par hémorragies. L’olfaction est une source principale d’évaluation des risques présents dans l’environnement pour les rongeurs, avec la perception des signaux chimiques des prédateurs ou signaux de toxicité des plants/nourriture. Cette perception olfactive peut être utilisé pour modifier l’utilisation de l’espace des rongeurs. L’objectif de cette thèse était l’identification des réponses comportementales aux messages chimiques importants (par exemple les signaux chimiques émis par les plantes et les prédateurs) dans l’écologie de la souris domestique (Mus musculus), avec l’utilisation de souches de laboratoire comme modèle des animaux sauvages. Nos résultats ont montré que la souris a évité de façon significative les signaux chimiques complexes du furet et un signal chimique ubiquitaire des plantes, lié à la maturation et la pourriture des aliments (l’éthanol). La protéine du chat Fel d 1, laquelle fait partie de la famille des sécrotoglobines et est un allergène majeur du chat, n’a pas modifié le comportement d’exploration de la souris ou son comportement de recherche et de consommation de nourriture. Le composant chimique des fèces de renard, le TMT a induit un évitement clair et des réponses de stress comme cela a été rapporté dans la littérature. De plus, j’ai fait une revue de la littérature pour évaluer et discuter les méthodes de contrôle des rongeurs d’un point de vue éthique, revue qui a démontré que les méthodes actuelles peuvent être considérés inhumaines et ne correspondent pas aux attentes actuelles de la société et aux standards sur le bien-être dans d’autres domaines comme les élevages de production ou les animaux de laboratoire. Ces résultats ouvrent des nouvelles voies de recherche afin d’identifier les composants chimiques du furet et des plantes liés au comportement d’évitement des rongeurs, les prochaines étapes utilisant des animaux sauvages à la fois en laboratoire et sur le terrain. / Rodent commensal species produce great damage in agriculture and urban areas. As invasive species they can endanger local species and are carriers and vectors of several important zoonoses. Control methods rely mainly on the use of warfarins, which can be inadvertently be taken up by untargeted species. Warfarins have also lost their efficacy in rodents due to the development of genetic resistance. In addition, these methods are considered inhumane as they cause a slow and painful death due to haemorrhages. Olfaction is a main source for environmental risk assessment by rodents, and it can be used to modify their use of space. My aim in this thesis was to identify behavioural reactions of the house mouse (Mus musculus), using laboratory strains as models of wild animals, to ecologically meaningful chemical messages, including predator and plant chemical olfactory cues. My results showed that mice avoided complex ferret olfactory cues and ethanol which is a ubiquitous chemical related to fruit rotting and ripening. The feline protein Fel d 1, which belongs to the secretoglobin family and is a major cat allergen in humans, did not elicit significant avoidance or alter foraging behaviour in mice. However, Trimethylthiazoline purified from fox faeces, elicited clear avoidance behaviour and stress responses. I carried out a bibliographic review to evaluate and discuss rodent pest control methods from an ethical standpoint. This literature showed that many of the current methods of pest control are considered inhumane, and do not tally with current society concerns and welfare standards in other domains such as farms or laboratory animals. These results raise new research questions to identify ferret and plant chemical compounds that can induce rodent avoidance, and to carry out next stage of research with wild animals both under laboratory and field conditions.

Recent research has focused on the non-lethal effects of predator intimidation and fear, dubbed non-consumptive effects (NCEs), in which prey actively change their behavior and habitat use in response to predator chemical cues. Although NCEs can have large impacts on community structure, many studies have ignored differences in predator population structure and properties of the natural environment that may modify the magnitude and importance of NCEs. Here, I investigated the roles of predator size and density (i.e. biomass), as well as habitat characteristics, on predator risk assessment and the magnitude of consumptive and NCEs using blue crabs, mud crabs, and oyster prey as a model system. Predation experiments between blue crabs and mud crabs demonstrated that blue crabs consume mud crabs; however, the consumptive effects were dependent upon blue crab body size and habitat type. When mud crabs were exposed to chemical cues from differing biomasses of blue crabs in laboratory mesocosms, mud crab activity and predation on oysters was decreased in response to high biomass treatments (i.e. large and multiple small blue crabs), but not to low biomass predators (i.e single small blue crab), suggesting that risk associated with predator size is perceptible via chemical cues and is based on predator biomass. Further experiments showed that the perception of risk and the magnitude of the NCEs were affected by the sensory cues available and the diet of the blue crab predator. The NCE based on blue crab biomass was also demonstrated in the field where water flow can disperse cues necessary for propagating NCEs. Properties of water flow were measured within the experimental design and during the experiment and confirmed cage environments were representative of natural conditions and that patterns in NCEs were not associated with flow characteristics. These results affect species conservation and commercial fisheries management and demonstrate that we cannot successfully predict NCEs without considering predator size structure and the contexts under which we determine predator risk.

Responses of tadpoles Bombina variegata (likely unexperienced with predator) to pulses of risk and safety were tested and compared with results of a bachelor thesis previously done on tadpoles Bufo bufo (species likely experienced with predator) to confirm the risk allocation hypothesis. Differences of overall activity of both species were not significant. Differences in activity between Bufo bufo and Bombina variegata tadpoles held in continual risk conditions were observed. Such behavior possibilities are discussed.

A good ecological status of streams and rivers is crucial for maintaining ecological functionality of running waters. Worldwide eutrophication threatens to change structure and function of freshwater ecosystems (Dodds et al., 2008). To reduce the symptoms of eutrophication in streams and rivers an additional approach, besides the reduction of external nutrient inputs from catchment areas, is needed. Therefore the goal has been set to transfer the approach of biomanipulation, which is widely accepted as tool in water quality management in lakes and reservoirs, to streams. The objective of this study was accordingly to analyse and evaluate some crucial preconditions for top-down control of stream food webs. For that purpose the present thesis examined effects of fish predation (stone loach and gudgeon) on grazer-periphyton interaction in small streams by assessing predator avoidance by benthic grazers, effects of benthic grazers on periphyton community composition during fish presence/absence and the possibility of top-down control on algal biomass by benthivorous fish.

A good ecological status of streams and rivers is crucial for maintaining ecological functionality of running waters. Worldwide eutrophication threatens to change structure and function of freshwater ecosystems (Dodds et al., 2008). To reduce the symptoms of eutrophication in streams and rivers an additional approach, besides the reduction of external nutrient inputs from catchment areas, is needed. Therefore the goal has been set to transfer the approach of biomanipulation, which is widely accepted as tool in water quality management in lakes and reservoirs, to streams. The objective of this study was accordingly to analyse and evaluate some crucial preconditions for top-down control of stream food webs. For that purpose the present thesis examined effects of fish predation (stone loach and gudgeon) on grazer-periphyton interaction in small streams by assessing predator avoidance by benthic grazers, effects of benthic grazers on periphyton community composition during fish presence/absence and the possibility of top-down control on algal biomass by benthivorous fish.