Orientador: Raghuvir Krishnaswamy Arni / Coorientador: José Osmar Gaspar / Banca: Marcelo Andrés Fossey / Banca: Priscila Belintani / Resumo: As atividades realizadas compreenderam a produção de cDNA utilizando primer anti-senso e RNA purificado, amplificação do gene codificador da proteína 25K do Cole latent virus (CoLV25K), purificação do fragmento amplificado, ligação em vetor de multiplicação pGEM-T, transformação em células competentes de Escherichia coli linhagem TOP 10, purificação do vetor, digestão enzimática com as enzimas Bam HI e Hind III, subclonagem no vetor de expressão pET28a, transformação em células competentes de E. coli linhagem BL21-RIL, sequenciamento do gene no vetor de expressão e expressão da proteína a 37 o C . Quando expressa a 37 ºC, a proteína, de 25 kDa, foi encontrada em sua totalidade nos corpos de inclusão. Dessa forma, a proteína foi purificada sob condição desnaturante (utilizando 8 M de uréia) e submetida à diálise para seu reenovelamento. Após o reenovelamento, a proteína foi concentrada para aproximadamente 3 mg/mL e foram realizadas medidas de dicroísmo circular, para verificar o seu conteúdo de estrutura secundária, e o espalhamento dinâmico de luz, para estimar a distribuição de tamanho das populações de partículas que estão presentes na solução. Os dados da deconvolução do experimento de dicroísmo circular indicam um percentual de 40-46% α-hélice, 12-14% folha-β, 15-22% voltas e 24-28% de outras estruturas, indicando que a proteína está estruturada; e os dados do espalhamento dinâmico de luz mostraram que a proteína encontra-se estável, monodispersa, mas apresenta um complexo de partículas que deve ser removido para que fique nas condições ideais de cristalização. Foram utilizadas também outras técnicas para tentar alcançar a solubilidade da proteína expressa: abaixando a temperatura... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The experiments performed were, the production of cDNA using anti-sense primers and purified RNA, amplification of the gene encoding the 25K protein of Cole latent virus (CoLV25K), purification of the amplified fragment, cloning in multiplication vector pGEM-T for cell transformation into competent Escherichia coli strain TOP 10, vector purification, enzymatic digestion using the enzymes Bam HI and Hind III, subcloning in expression vector pET28a, transformation into competent cells of E. coli strain BL21-RIL, sequencing of the gene cloned into the expression vector and protein expression at 37 o C. When expressed at 37 °C, the protein with a molecular mass of 25 kDa was detected in inclusion bodies. Thus, the protein was purified under denaturing conditions (using 8 M urea) and subjected to dialysis to stimulate refolding. After refolding, the protein was concentrated to approximately 3 mg / mL and the circular dichroism assay was performed to verify the content of secondary structure, and dynamic light scattering, to estimate the size distribution of particle populations which are present in solution. The data from the deconvolution of circular dichroism experiments indicate a percentage of 40-46% α-helix, 12-14% β-sheet, 15-22% turns and 24-28% of other structures, indicating a structured protein; and the data of the dynamic light scattering showed that the protein is stable, monodispersive, but forms a large complex of particles which must be separated to before crystallization experiments. Other techniques were used to solubilize the expressed protein: lowering the temperature of expression to 18 °C, using the method... (Complete abstract click electronic access below) / Mestre

Kaffesump ingår i kategorin matavfall- och svinn och varje år konsumerar hela Sverige cirka 79,7 tusen ton kaffe per år. Det innebär att enormt mycket kaffesump slängs och betraktas som avfall. Kaffesumpens innehåll är en resurs och kan utnyttjas inom flera olika områden. Kaffebranschen behöver ställa om från en linjär- till en cirkulär modell.  Syftet med denna studie är att, ur ett cirkulärt designperspektiv, undersöka huruvida avfall i form av kaffesump kan vara en resurs i en hållbar utveckling på svensk marknad.  Den här studien avser bidra till ökad kunskap om kaffesump som resurs, samt skapa en efterfrågan på produkter av kaffesump av konsumenter såväl som producenter. Detta för att bidra till en hållbar konsumtion i form av kunskap om- och utförande av en cirkulär kaffebransch med koppling till de globala målen 12 Hållbar konsumtion och produktion och 13 Bekämpa klimatförändringarna.  Huvudresultatet i denna studie är att kaffesump har stor potential för att vara en del i cirkulära processer, som bidrar till hållbarhet på den svenska marknaden.

International Telemetering Conference Proceedings / October 25-28, 1999 / Riviera Hotel and Convention Center, Las Vegas, Nevada / We present a novel communications link concept. This system offers the potential of low data rate full-duplex communications between earth and LEO. We will present a detailed link model for this system.

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The present study proposes a simple and relatively complete displacement field, which, with the finite element formulation, can be employed in the analyses of the free vibration of circular and annular plates, the vibration with preloading in the radial direction, which is studied quite often, and the vibration with preloading under twisting, which has not yet appeared in the literature. The present results of the free vibration and the vibration under compressive stresses for circular and annular plates with different boundary conditions and various aspect ratios will be compared with those by ANSYS and other research. From the comparisons, we know that the present method does not need a fine mesh to get accurate natural frequencies of the plates. As to the vibration for the bar or tube with pretorsional loading, coupled vibration modes in the r-z plane and £c direction, it can be revealed by the present method, and the appearance of these vibration modes may be due to the coupling terms from the proposed displacement field. Also, these vibration modes have not been shown before.

This thesis studies the circular chromatic number of Kneser graphs. It was known that if m is greater than 2n^{2}(n-1), then the Kneser graph KG(m,n) has its circular chromatic number equal its chromatic number . In particular, if n = 3, then KG(m,3) has its circular chromatic number equal its chromatic number when m is greater than 36. In this thesis, we improve this result by proving that if m is greaer than 24, then chi_c(KG(m,3)) = chi(KG(m,3)).

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