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The Global ETD Search service is a free service for researchers
to find electronic theses and dissertations. This service is
provided by the
Networked Digital Library of Theses and Dissertations.
Our metadata is collected from universities around the world. If you manage a university/consortium/country archive and want to be added, details can be found on the NDLTD website.
Search results
Showing 1 to 5 of 5 (0.018 seconds)| 1 |
CLC-3 a Putative Gamma VGCC Sub-unit Homologue in the Worm, <i>C. Elegans</i>Melnik-Martinez, Katya Verushka 05 March 2008 (has links)
No description available.
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Caractérisation fonctionnelle de canaux chlorure de la famille des ClCSchmieder, Sandra 25 February 2000 (has links) (PDF)
Les canaux chlorure sont impliqués dans divers processus indispensables à la vie cellulaire, tel que la stabilisation du potentiel de membrane, la régulation du volume ou du pH, ainsi que dans les transports transépithéliaux de sels. Depuis une douzaine d'années, l'application de la biologie moléculaire à l'étude des canaux chlorure a permis d'identifier plusieurs familles de gènes. La variété fonctionnelle des canaux chlorure peut donc s'expliquer par leur diversité moléculaire. Actuellement, la détermination des rôles physiologiques des canaux chlorure clonés constitue un enjeu majeur de leur étude.<br /><br />Récemment, une stratégie de clonage par homologie nous a permis d'isoler deux nouveaux membres de la famille des ClC, canaux chlorure dépendants du voltage: le xClC-5 et le xClC-3. La caractérisation fonctionnelle et la détermination de la localisation tissulaire et cellulaire de ces protéines a constitué l'objectif de notre étude. Pour cela, l'ovocyte de xénope et la lignée cellulaire HEK 293 ont été utilisés comme systèmes d'expression. L'analyse électrophysiologique, menée par une technique de voltage-clamp en double micro-électrodes, nous a permis de caractériser le xClC-5 du point de vue électrophysiologique et de déterminer sa sensibilité au pH, aux ions métalliques et aux inhibiteurs de tyrosine kinases. L'utilisation d'anticorps nous a permis d'étudier la glycosylation des protéines et d'examiner leur distribution tissulaire et/ou leur localisation cellulaire. Nos résultats sont discutés par rapport à ceux d'autres équipes, en prêtant une attention particulière aux modèles fonctionnels possibles, qui émergent actuellement pour les protéines ClC-5 et ClC-3.
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The Role of Chloride Channels in Regulation of Pulmonary Artery Smooth Muscle Cell ProliferationLiang, Wenbin 19 November 2013 (has links)
Pulmonary arterial hypertension (PAH) is a rare but fatal disease with an annual mortality rate of 15% despite current therapies. Uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs) results in adverse vascular remodeling contributing to PAH. Understanding the mechanisms of PASMC proliferation may identify new targets for treatment. Chloride currents/channels (ICl) are expressed in PASMCs and their roles in proliferation have been suggested based on their importance in resting membrane potential and cell volume regulation. The present study explored the role of ICl in proliferation in rat and human PASMCs. We found that either nonspecific ICl inhibitors (DIDS or NPPB) or a putative specific blocker of swelling-activated ICl (ICl,swell) reduced proliferation of PASMCs cultured in serum-containing media. Patch-clamp studies showed that proliferating PASMCs had increased baseline ICl and ICl,swell in association with depolarized membrane potentials. Quantitative real-time RT-PCR studies identified expressions of CLC-3, a candidate gene of ICl,swell, and several other CLC genes in proliferating PASMCs. While selective knockdown of CLC-3 with lentiviral shRNA reduced PASMC proliferation, it had no effect on ICl,swell. These findings are consistent with the conclusion that ICl regulate proliferation of PASMCs and suggest that selective ICl inhibition may be useful in treating pulmonary arterial hypertension.
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The Role of Chloride Channels in Regulation of Pulmonary Artery Smooth Muscle Cell ProliferationLiang, Wenbin 19 November 2013 (has links)
Pulmonary arterial hypertension (PAH) is a rare but fatal disease with an annual mortality rate of 15% despite current therapies. Uncontrolled proliferation of pulmonary artery smooth muscle cells (PASMCs) results in adverse vascular remodeling contributing to PAH. Understanding the mechanisms of PASMC proliferation may identify new targets for treatment. Chloride currents/channels (ICl) are expressed in PASMCs and their roles in proliferation have been suggested based on their importance in resting membrane potential and cell volume regulation. The present study explored the role of ICl in proliferation in rat and human PASMCs. We found that either nonspecific ICl inhibitors (DIDS or NPPB) or a putative specific blocker of swelling-activated ICl (ICl,swell) reduced proliferation of PASMCs cultured in serum-containing media. Patch-clamp studies showed that proliferating PASMCs had increased baseline ICl and ICl,swell in association with depolarized membrane potentials. Quantitative real-time RT-PCR studies identified expressions of CLC-3, a candidate gene of ICl,swell, and several other CLC genes in proliferating PASMCs. While selective knockdown of CLC-3 with lentiviral shRNA reduced PASMC proliferation, it had no effect on ICl,swell. These findings are consistent with the conclusion that ICl regulate proliferation of PASMCs and suggest that selective ICl inhibition may be useful in treating pulmonary arterial hypertension.
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On the chloride dependence of vesicular glutamate transport / Über die Chloridabhängigkeit des vesikulären GlutamattransportsSchenck, Stephan 26 June 2009 (has links)
No description available.
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