Following Merton (1942) science studies has moved from the philosophy of science to a more sociologically minded analysis of scientific activity. This largely involves a shift away from questions that bear on the context of justification – a question of rationality and philosophy, to those that deal with the context of discovery. This thesis investigates changes in science studies in three papers: sociocultural evolutionary theories of scientific change; general trends in science studies - especially concerning the sociology of science; and a principle component analysis (PCA) that details the development and interaction between research programmes in science studies. This thesis describes the proliferation of research programmes in science studies and uses evolutionary theory to make sense of the pattern of change.

Quantification of natural organic matter (NOM) in water is limited by the complex and varied nature of compounds found in natural waters. Current characterization techniques, which identify and quantify fractions of NOM, are often expensive and time consuming suggesting the need for rapid and accurate characterization methods. In this work, principal component analysis of fluorescence excitation-emission matrices (FEEM-PCA) was investigated as a NOM characterization technique. Through the use of jar tests and disinfection by-product formation tests, FEEM-PCA was shown to be a good surrogate for disinfection by-product precursors. FEEM-PCA was also applied in order to characterize differences in humic-like, protein-like, and Rayleigh scattering between multiple source waters and due to differing treatment processes. A decrease in Rayleigh scattering influence was observed for a deep lake intake, and multiple processes were found to significantly affect humic-like substances, protein-like, and Rayleigh scattering fractions.

Following Merton (1942) science studies has moved from the philosophy of science to a more sociologically minded analysis of scientific activity. This largely involves a shift away from questions that bear on the context of justification – a question of rationality and philosophy, to those that deal with the context of discovery. This thesis investigates changes in science studies in three papers: sociocultural evolutionary theories of scientific change; general trends in science studies - especially concerning the sociology of science; and a principle component analysis (PCA) that details the development and interaction between research programmes in science studies. This thesis describes the proliferation of research programmes in science studies and uses evolutionary theory to make sense of the pattern of change.

Quantification of natural organic matter (NOM) in water is limited by the complex and varied nature of compounds found in natural waters. Current characterization techniques, which identify and quantify fractions of NOM, are often expensive and time consuming suggesting the need for rapid and accurate characterization methods. In this work, principal component analysis of fluorescence excitation-emission matrices (FEEM-PCA) was investigated as a NOM characterization technique. Through the use of jar tests and disinfection by-product formation tests, FEEM-PCA was shown to be a good surrogate for disinfection by-product precursors. FEEM-PCA was also applied in order to characterize differences in humic-like, protein-like, and Rayleigh scattering between multiple source waters and due to differing treatment processes. A decrease in Rayleigh scattering influence was observed for a deep lake intake, and multiple processes were found to significantly affect humic-like substances, protein-like, and Rayleigh scattering fractions.

Antioxidants are molecules capable of stabilizing and preventing oxidation. Certain peptides, protein hydrolysates, have shown antioxidant capacities, which are obtained once liberated from the native protein structure. Soy protein isolates (SPI) were enzymatically hydrolyzed by pepsin and pancreatin mixtures. The soy protein hydrolysates (SPH) were fractionated with sequential ultrafiltration (UF) and nanofiltration (NF) membrane steps. Heat pre-treatment of SPI at 95 degrees celsius (C) for 5 min prior to enzymatic hydrolysis was investigated for its effect on peptide distribution and antioxidant capacity. SPH were subjected to UF with a 10 kDa molecular weight cut off (MWCO) polysulfone membrane. UF permeate fractions (lower molecular weight than 10 kDa) were fractionated by NF with a thin film composite membrane (2.5 kDa MWCO) at pH 4 and 8. Similar peptide content and antioxidant capacity (α=0.05) were obtained in control and pre-heated SPH when comparing the respective UF and NF permeate and retentate fractions produced. FCR antioxidant capacities of the SPH fractions were significantly lower than their ORAC antioxidant capacities, and the distribution among the UF and NF fractions was generally different. Most UF and NF fractions displayed higher antioxidant capacities when compared to the crude SPI hydrolysates, showing the importance of molecular weight on antioxidant capacity of peptides. The permeate fractions produced by NF at pH 8 displayed the highest antioxidant capacity, expressed in terms of Trolox equivalents (TE) per total solids (TS): 5562 μmol TE/g TS for control SPH, and 5187 μmol TE/g TS for pre-heated SPH. Due to the improvement in antioxidant capacity of peptides by NF at pH 8, the potential for NF as a viable industrial fractionation process was demonstrated. Principal component analysis (PCA) of fluorescence excitation-emission matrix (EEM) data for UF and NF peptide fractions, followed by multi-linear regression analysis, was assessed for its potential to monitor and identify the contributions to ORAC and FCR, two in vitro antioxidant capacity assays, of SPH during membrane fractionation. Two statistically significant principal components (PCs) were obtained for UF and NF peptide fractions. Multi-linear regression models (MLRM) were developed to estimate their fluorescence and PCA-captured ORAC (ORAC-FPCA) and FCR (FCR-FPCA) antioxidant capacities. The ORAC-FPCA and FCR-FPCA antioxidant capacities for NF samples displayed strong, linear relationships at different pH conditions (R-squared>0.99). Such relationships are believed to reflect the individual and relative combined contributions of tryptophan and tyrosine residues present in the SPH fractions to ORAC and FCR antioxidant capacities. Therefore, the proposed method provides a tool for the assessment of fundamental parameters of antioxidant capacities captured by ORAC and FCR assays.

In pattern recognition, when data has different structures in different parts of the input space, fitting one global model can be slow and inaccurate. Learning methods can quickly learn the structure of the data in local regions, consequently, offering faster and more accurate model fitting. Breaking training data set into smaller subsets may lead to curse of dimensionality problem, as a training sample subset may not be enough for estimating the required set of parameters for the submodels. Increasing the size of training data may not be at hand in many situations. Interestingly, the data in local regions becomes more correlated. Therefore, by decorrelation methods we can reduce data dimensions and hence the number of parameters. In other words, we can find uncorrelated low dimensional subspaces that capture most of the data variability. The current subspace modelling methods have proved better performance than the global modelling methods for the given type of training data structure. Nevertheless these methods still need more research work as they are suffering from two limitations 2 There is no standard method to specify the optimal number of subspaces. &sup2 / There is no standard method to specify the optimal dimensionality for each subspace. In the current models these two parameters are determined beforehand. In this dissertation we propose and test algorithms that try to find a suboptimal number of principal subspaces and a suboptimal dimensionality for each principal subspaces automatically.

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Type Ia supernovae (SNe Ia) are currently the best probe of the expansion history of the universe. Their usefulness is due chiefly to their uniformity between supernovae (SNe). However, there are some slight variations amongst SNe that have yet to be understood and accounted for. The goal of this work is to uncover relationships between the spectral features and the light curve decline rate, [delta]m₁₅. Wavelet decomposition has been used to develop a new spectral index to measure spectral line strengths independent of the continuum and easily corrected for noise. This new method yields consistent results without the arbitrary uncertainties introduced by current methods and is particularly useful for spectra which do not have a clearly defined continuum. These techniques are applied to SN Ia spectra and correlations are found between the spectral features and light curve decline rate. The wavelet spectral indexes are used to measure the evolution of spectral features which are characterized by 3 or 4 parameters for the most complicated evolution. The three absorption features studied here are associated with sulfur and silicon and all show a transition in strength between 1 to 2 weeks after B-band maximum. Pearson correlation coefficients between spectral features and [delta]m₁₅ are found to be significant within a week of maximum brightness and 3 to 4 weeks post-maximum. These correlations are used to determine the principal components at each epoch among the set of SN spectra in this work. The variation contained in the first principal component (PC1) is found to be greater than 60% to 70% for most epochs and reaching as high as 80% to 90% for epochs with the highest correlations. The same first principal component can be used to relate spectral feature strengths to the decline rate. These relations were used to estimate a SN light curve decline rate from a set of spectra taken over the course of the explosion, from a single spectrum, or from even a single spectral feature. These relationships could be used for future surveys to estimate spectral characteristics from light curve data, such as photometric redshift.

This thesis presents an investigation of statistical analytical methods applied to the analysis of the shape of the arterial pressure waveform. The arterial pulse is analysed by a selection of both supervised and unsupervised methods of learning. Supervised learning methods are generally better known as regression. Unsupervised learning methods seek patterns in data without the specification of a target variable. The theoretical relationship between arterial pressure and wave shape is first investigated by study of a transmission line model of the arterial tree. A meta-database of pulse waveforms obtained by the SphygmoCor"??" device is then analysed by the unsupervised learning technique of Self Organising Maps (SOM). The map patterns indicate that the observed arterial pressures affect the wave shape in a similar way as predicted by the theoretical model. A database of continuous arterial pressure obtained by catheter line during sleep is used to derive supervised models that enable estimation of arterial pressures, based on the measured wave shapes. Independent component analysis (ICA) is also used in a supervised learning methodology to show the theoretical plausibility of separating the pressure signals from unwanted noise components. The accuracy and repeatability of the SphygmoCor?? device is measured and discussed. Alternative regression models are introduced that improve on the existing models in the estimation of central cardiovascular parameters from peripheral arterial wave shapes. Results of this investigation show that from the information in the wave shape, it is possible, in theory, to estimate the continuous underlying pressures within the artery to a degree of accuracy acceptable to the Association for the Advancement of Medical Instrumentation. This could facilitate a new role for non-invasive sphygmographic devices, to be used not only for feature estimation but as alternatives to invasive arterial pressure sensors in the measurement of continuous blood pressure.