Thesis (Ph. D.)--Ohio State University, 2007. / Title from first page of PDF file. Includes bibliographical references (p. 129-159).

Thesis (Ph. D.)--University of Missouri-Columbia, 2000. / Typescript. Vita. Includes bibliographical references (leaves 150-177). Also available on the Internet.

Existing Building Automation Systems (BASs) and Building Automation Networks (BANs) have been shown to have serious cybersecurity problems. Due to the safety-critical and interconnected nature of building subsystems, local and network access control needs to be finer grained, taking into consideration the varying criticality of applications running on heterogeneous devices. In this paper, we present a secure communication framework for BASs that 1) enforces rich access control policy for operating system services and objects, leveraging a microkernel-based architecture; 2) supports fine-grained network access control on a per-process basis; 3) unifies the security control of inter-device and intra-device communication using proxy processes; 4) tunnels legacy insecure communication protocols (e.g., BACnet) through a secure channel, such as SSL, in a manner transparent to legacy applications. We implemented the framework on seL4, a formally verified microkernel. We conducted extensive experiments and analysis to compare the performance and effectiveness of our communication systems against a traditional Linux-based implementation of the same control scenario. Our experiments show that the communication performance of our system is faster or comparable to the Linux-based architecture in embedded systems.

Made available in DSpace on 2014-06-11T19:26:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2012-09-03Bitstream added on 2014-06-13T20:53:59Z : No. of bitstreams: 1 costa_pr_me_botib.pdf: 664900 bytes, checksum: d4b731cf5ba2463de35082d25d1fdb1e (MD5) / Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / A transcrição realizada pela RNA polimerase (RNAP) é um processo cuidadosamente controlado no desenvolvimento e na manutenção das funções vitais dos organismos. O desenvolvimento de novas técnicas e equipamentos para seu estudo, como as técnicas de pinça ótica ou magnética, a microscopia de força atômica e a fiuorescência de molécula única, complementaram os resultados dos estudos bioquímicos tradicionais e nos levaram a um maior entendimento do processo. A ocorrência de pausas em sítios específicos durante o alongamento, já observadas na década de 80, passou a ser estudada com maior interesse devido a sua importância biológica: acredita-se que essas pausas assegurem que a transcrição e a tradução ocorram simultaneamente em bactérias, permitam o dobramento correto das estruturas secundárias e terciárias do R A, facilitem a ligação de reguladores de alongamento e precedam a etapa de terminação transcricional. Modelos teóricos baseados na estabilidade termodinâmica do complexo de alongamento transcricional foram bem sucedidos na previsão da cinética do alongamento. Seus resultados indicaram que a RNAP pode ser vista como um motor molecular e sua motilidade possui características do modelo de catraca browniana. Entretanto, esses modelos consideram a presença de apenas uma polimerase realizando a transcrição. Experimentos recentes mostraram que a ocorrência de colisões entre essas enzimas durante a transcrição múltipla de um mesmo gene altera seu comportamento. Baseados nesses resultados, propomos a generalização de um dos modelos estocásticos que consideram a sequência molde para o estudo desse fenômeno. Em nossa aproximação, colisões entre as moléculas RNAP modificam a taxa de ocorrência da transcrição. A implementação do modelo foi realizada em... / The transcription of the information encoded within the DNA to the RNA molecule is exquisitely controlled during the development of the organisms and to its vital functions and has as the protagonist the RNA polymerase enzyme (RNAP). The development of single-molecule techniques, such as the magnetic and optical tweezers, atomic-force microscopy and single-molecule uorescence, increased our understanding of the process, complementing traditional biochemical studies. The non-homogeneity of the RNAP movement due to the occurrence of \pauses at speci c sites during elongation was revealed using electrophoresis gels. It is believed that these pauses ensure concurrency between transcription and translation in bacteria, allow the correct folding of RNA secondary and tertiary structures, facilitate the binding of regulating factors during elongation and preceding the transcriptional termination step. Theoretical models have been proposed to explain and predict the RNAP kinetics during the polymerization. Models based on the thermodynamic stability of the transcription elongation complex recover much of the kinetics and indicate that its movement has a Brownian ratchet mechanism. However, experiments showed that if more than one RNAP molecule initiate from the same promoter, their behavior changes and new phenomenona are observed. We proposed and implemented a theoretical model that considers collisions between RNAP molecules and predicts their cooperative behavior during multi-round transcription. The model generalizes a stochastic sequence-dependent model. In our approach, collisions between elongating enzymes modify their transcription rate values. We performed the simulations in Mathematica and compared the results of the single and the multiple-molecule transcription with experimental... (Complete abstract click electronic access below)

Orientador: José Luis Bendicho Beired / Banca: Carlos Alberto Sampaio Barbosa / Banca: Maria Helena Rolim Capelato / Resumo: Na Argentina - principalmente no final do século XIX - foi freqüente a utilização da etnicidade pelas elites intelectuais como uma das maneiras de formular questões pendentes ao processo de formação da pátria. Os cientificistas argentinos utilizaram-se, muitas vezes, de apelações biologistas e de concepções do darwinismo social, produzindo a teoria do "crisol de raças" como mecanismo de controle social e étnico para nacionalizar as massas chegadas durante o processo de imigração européia, a partir de 1880. Neste trabalho, buscaremos compreender as relações existentes entre Carlos Octavio Bunge (1875-1918) e José Ingenieros (1877-1925), partindo da análise de sua produção intelectual do início do século XX e de suas publicações em revistas do período - Revista de Derecho, Historia y Letras, Revista de Filosofia, Cultura, Ciencias y Educación (1915 - 1929) e da revista El Monitor de la Educación Común (1881 - 1965) - uma vez que estes compartilhavam das mesmas idéias étnicas e da preocupação em decifrar a história nacional, sendo considerados, por isso, pertencentes à corrente cientificista. / Abstract: In Argentina - mainly in the end of 19th century - It was often the utilization of ethnicity by the intellectual elite as one of the ways to formulate pending questions about the country formation process. The argentineans cientificists used, several times, biologist appeals and social darwinism conceptions, producing the theory of "racial mixture" as mechanism of social and ethnic control to nationalize the arriving masses during the European immigration process, from 1880. In this work, we will focus the understanding of the relationships that existed among Carlos Octavio Bunge (1875-1918) and José Ingenieros (1877-1925), studying the analysis of his intellectual production of the beginning of the 20th century and the publications in magazines of the period - Revista de Derecho, Historia y Letras, Revista de Filosofia, cultura, ciencias y educación (1915 - 1929) and the magazine "El monitor of la educación común" (1881 - 1965) - once they shared the same ethnic ideas and the concern in deciphering the national history, being considered, for that, belonging to the cientificist current. / Mestre

Orientador: Ney Lemke / Banca: Scheila de Ávila e Silva / Banca: José Luiz Rybarczyk Filho / Resumo: A transcrição realizada pela RNA polimerase (RNAP) é um processo cuidadosamente controlado no desenvolvimento e na manutenção das funções vitais dos organismos. O desenvolvimento de novas técnicas e equipamentos para seu estudo, como as técnicas de pinça ótica ou magnética, a microscopia de força atômica e a fiuorescência de molécula única, complementaram os resultados dos estudos bioquímicos tradicionais e nos levaram a um maior entendimento do processo. A ocorrência de "pausas" em sítios específicos durante o alongamento, já observadas na década de 80, passou a ser estudada com maior interesse devido a sua importância biológica: acredita-se que essas pausas assegurem que a transcrição e a tradução ocorram simultaneamente em bactérias, permitam o dobramento correto das estruturas secundárias e terciárias do R A, facilitem a ligação de reguladores de alongamento e precedam a etapa de terminação transcricional. Modelos teóricos baseados na estabilidade termodinâmica do complexo de alongamento transcricional foram bem sucedidos na previsão da cinética do alongamento. Seus resultados indicaram que a RNAP pode ser vista como um motor molecular e sua motilidade possui características do modelo de catraca browniana. Entretanto, esses modelos consideram a presença de apenas uma polimerase realizando a transcrição. Experimentos recentes mostraram que a ocorrência de colisões entre essas enzimas durante a transcrição múltipla de um mesmo gene altera seu comportamento. Baseados nesses resultados, propomos a generalização de um dos modelos estocásticos que consideram a sequência molde para o estudo desse fenômeno. Em nossa aproximação, colisões entre as moléculas RNAP modificam a taxa de ocorrência da transcrição. A implementação do modelo foi realizada em ... (Resumo completo, clicar acesso eletrônico abaixo) / Abstract: The transcription of the information encoded within the DNA to the RNA molecule is exquisitely controlled during the development of the organisms and to its vital functions and has as the protagonist the RNA polymerase enzyme (RNAP). The development of single-molecule techniques, such as the magnetic and optical tweezers, atomic-force microscopy and single-molecule uorescence, increased our understanding of the process, complementing traditional biochemical studies. The non-homogeneity of the RNAP movement due to the occurrence of \pauses" at speci c sites during elongation was revealed using electrophoresis gels. It is believed that these pauses ensure concurrency between transcription and translation in bacteria, allow the correct folding of RNA secondary and tertiary structures, facilitate the binding of regulating factors during elongation and preceding the transcriptional termination step. Theoretical models have been proposed to explain and predict the RNAP kinetics during the polymerization. Models based on the thermodynamic stability of the transcription elongation complex recover much of the kinetics and indicate that its movement has a Brownian ratchet mechanism. However, experiments showed that if more than one RNAP molecule initiate from the same promoter, their behavior changes and new phenomenona are observed. We proposed and implemented a theoretical model that considers collisions between RNAP molecules and predicts their cooperative behavior during multi-round transcription. The model generalizes a stochastic sequence-dependent model. In our approach, collisions between elongating enzymes modify their transcription rate values. We performed the simulations in Mathematica and compared the results of the single and the multiple-molecule transcription with experimental... (Complete abstract click electronic access below) / Mestre

Doctorat en sciences médicales / info:eu-repo/semantics/nonPublished

Anti-oestrogens act via the oestrogen receptor whether they compete with the hormone for binding to the receptor and therefore interfere with DNA binding or inhibit transcriptional activity. These receptors exist as a large 85 complex and/or a small 45 form on sucrose density gradients. High performance ion-exchange chromatography has confirmed that the oestrogen and progestin complex is present in various isoforms. Progestin receptor heterogeneity could be influenced by the presence of oestrogens and anti-oestrogens in the culture media of hormone-dependent neoplastic cells. Cell culture methods offer the opportunity to test effects of specified components in repeated experiments on a homogeneous population of cells. MCF-7 and T47-D human breast cancer cell lines were conditioned to grow in a serum-free environment. There was no difference in cell proliferation rates, nor in their oestrogen or progestin receptor levels when compared to the same cells grown in conventional media. Receptors were present mainly in the large molecular 85 form. Both the MCF-7 and T47-D breast cancer cells showed an increase in proliferation rate with the addition of oestrogen or diethylstilbestrol. There was a corresponding loss of progestin receptor levels and an alteration in the high performance ion-exchange isoforms. Flow cytometry confirmed differences in the S-phase components of the cells following exposure to oestrogens. The proliferation rates of the cell lines as well as their progestin receptor levels decreased when treated with tamoxifen or the hydroxylated tamoxifen. There were marked changes on high performance ion-exchange chromatography profiles. DNA ploidy and S-phase showed signs of toxicity and there was an increase in cellular debris. The MCF-7 and T47-D human breast cancer cell line retained response to antioestrogen saturation.

Body ownership can be studied via the rubber hand illusion (RHI), in which an artificial limb can be perceived as belonging to oneself. In the so-called moving RHI paradigm, both body ownership and sense of agency, induced by self-produced movements, can be investigated. The key question of this approach is whether movements generated by oneself increase the illusion of body ownership. Thus far, the results from moving RHI studies are inconsistent.This has led to uncertainty regarding the influences of the motor control mechanism on body ownership. Therefore, this study will present the first meta-analysis on moving RHI to estimate the illusory effectiveness induced by self-produced movements. A total of 23 experimental comparisons with 821 subjects were included in the meta-analysis. The results showed that the overall illusory effect induced by self-produced movements was superior toits control (e.g., asynchronous active movements) (Hedge’s g = 1.38, p < 0.001). However, due to dissimilarity in results between the studies, the sample size in the meta-analysis may not represent the general population. The subgroup analysis showed that studies using physical hands, such as wooden hands, yielded the largest effect compared to studies using a virtual projected hand or a video recorded image of the participant’s own hands. It can be speculated whether a three-dimensional hand with “realness” has an illusory advantage compared to hands presented in virtual or video image settings. Future studies need to apply aunified framework, particularly in experimental setups and measurements. This would obtain consistent results of the strength of the illusion within the moving RHI paradigm.

Successful companies that are founded and established in Sweden are likely to at some point look to expand outside of Sweden. When they do so they will need to adapt the company to the environment and circumstances that are present in the local market they are expanding to. The main purpose of this thesis is to investigate Swedish companies’ use of control mechanisms when running a subsidiary in Australia. Applying the theoretical framework by Malmi and Brown (2008), four Swedish companies operating in the Australian market were selected. Ikea using culture as an asset make them an interesting comparison to three highly reputable tech-companies that both differ from Ikea in age and size. It was found that cultural control mechanisms were heavily featured among the respondents' respective companies, and reward and compensation control mechanisms were altered the most when operating in Australia compared to Sweden. / Framgångsrika bolag grundade och etablerade i Sverige kommer sannolikt att någon gång expandera utanför Sverige. När de gör det kommer de att behöva anpassa företaget till den miljö och de omständigheter som råder på den lokala marknaden de expanderar till. Huvudsyftet med denna uppsats är att undersöka hur svenska bolag styr sina kontrollmekanismer när de driver en filial i Australien. Fyra svenska företag på den australienska marknaden har valts att studeras utifrån det teoretiska ramverket av Malmi and Brown (2008). Ikeas användning av svensk kultur som en tillgång gör det till en intressant jämförelse med tre välrenommerade teknikföretag, som både skiljer sig från Ikea när det gäller ålder och storlek. Det visade sig att kulturella kontrollmekanismer var flitigt förekommande bland respondenternas respektive företag, och belönings- och kompensations-kontrollmekanismerna förändrades mest när man verkade i Australien i jämförelse med Sverige.