Contemporary row-crop practices in the mid-southern, USA contribute to declining water tables and impairment of downstream waters, threatening the sustainability of irrigated agriculture and vital ecosystem services. This research was conducted to determine if various conservation practices and technologies can economically reduce negative environmental consequences of regional corn (Zea mays L.) production. The environmental, agronomic, and economic effects of implementing cover crops, manipulating traffic pattern, and integrating irrigation water management (IWM) technologies (computerized hole selection, surge irrigation, and soil moisture sensors) were investigated at either the small plot or field scale in the Delta region of Mississippi. Cover crop effects on runoff, water quality, corn grain yield, and net returns were primarily neutral or negative. Under furrow irrigation, cover crops did not affect runoff or nitrogen and phosphorus transport, but decreased erosion 16%. No cover crop decreased rainfall-induced runoff, erosion, or N and P transport. Over the four years of the experiment, cover crops maintained or decreased yield, net returns, and water use efficiency. Eliminating equipment traffic from furrows largely maintained or reduced runoff and subsequent contaminant transport. For both furrow irrigation and simulated rainfall, runoff and erosion were consistently less from nontraffic furrows than traffic furrows. Traffic effects on nutrient transport was more variable. Transport of some phosphorus constituents was decreased in the absence of furrow traffic; however, rainfall-induced nitrogen transport was greater from nontraffic furrows during the late-season event. Irrigation water management technologies improved corn grain yield, net returns, and irrigation water use efficiency across multiple locations in the mid-southern USA. Relative to standard production practices, implementing IWM technologies reduced total water applied 40% and increased corn grain yield 6.5 bu acre-1, which constituted a 51% increase in irrigation water use efficiency. The decrease in cost for irrigation water applied and increase in returns from yield gains exceeded the cost of the IWM technologies and resulted in an increase in net returns across a range of pumping depth and diesel price scenarios. Of the evaluated conservation strategies, reducing equipment traffic and implementing IWM technologies will most consistently improve the sustainability of irrigated agriculture and ecosystem services in the mid-southern USA.

Improved N management is required to enhance crop productivity, while reducing concomitant losses. Research was conducted for 4-y studying winter cover crops. Three legume species grown alone or with either cereal rye (Secale cereale) or ‘Tillage Radish®’ (Raphanus sativus L.) were used to quantify cover crop biomass and N content plus their effects on corn grain yield and N recovery. The effects of these cropping systems on selected soil health indicators was also determined. Rye bicultures enhanced biomass production, but antagonistically affected corn performance. Radish inclusion resulted in equal or greater cover crop N than rye. In year 3, the addition of radish across legume species increased corn N content (10.6 kg ha-1) and grain yield (1050 kg ha-1). Although cover crops did not affect soil bulk density, both bicultures increased soil C/N. The legume-radish association offers a novel practice towards improving crop performance and soil quality.

La alta frecuencia de cultivos de oleaginosas con moderados aportes y persistencia de residuos podría limitar la normal productividad de los suelos. La inclusión de cultivos invernales (utilizados como cultivos de cobertura, CC) en la región semiárida y subhúmeda pampeana sería una alternativa para proveer de residuos ricos en C y promover al desarrollo y al mantenimiento de la cobertura de los suelos. Pero se reconoce que el consumo hídrico de éstos podría interferir en la oferta de agua para el cultivo siguiente. El objetivo de este estudio fue evaluar la eficiencia en la producción de materia seca (MS) de cereales de invierno utilizados como CC y sus efectos sobre los contenidos de agua útil y nitratos en suelos diferenciados por el régimen hídrico. Los estudios se desarrolla-ron en 4 sitios experimentales [Luan Toro (LP) (Haplustol Entico, precipitación 624 mm), Dorila (LP) (Haplustol Entico, precipitación 873 mm), General Villegas (BA) (Hapludol Thapto Árgico, precipitación 906 mm) y General Pinto (BA) (Argiudol Típico, precipitación 1116 mm)]. Los tratamientos fueron: Secale cereale (C), Lolium multiflorum (R), Avena sativa (A) y un tratamiento Testigo, sin CC (T). La producción de MS al momento del secado de los CC varió entre 1226 y 10500 kg ha-1. Se observó una gran influencia del régimen hídrico de los suelos sobre la producción del MS de los CC. El contenido de agua total al momento de la siembra explicó el 57 % de la variabilidad de la producción de MS, mientras que el contenido de agua total en el suelo a la siembra más las precipitaciones durante el ciclo de desarrollo de los CC, explicó el 89 % de la variabilidad. En todos los sitios el centeno produjo la mayor cantidad de MS, seguida por la avena y el rye grass. La mayor variación se dio dentro del mismo sitio, entre los años de evaluación. El uso consuntivo hasta el momento del secado varió entre 88 y 299 mm. La especie con mayor eficiencia de uso de agua fue el centeno, alcanzando su máximo valor en el sitio de General Pinto (54 kg MS mm-1). Los valores de resistencia a la penetración fueron mayores bajo los tratamientos con CC y tuvieron una relación significativa con los contenidos hídricos del suelo. Sólo en el sitio Dorila los mayores valores fueron registrados en el tratamiento T, sin comprobarse relación significativa entre variaciones en la RP y los contenidos hídricos del suelo. Los contenidos de N-NO3- en el suelo fueron mayores en los tratamientos testigos, independientemente del sitio, en tanto que los contenidos de N de la biomasa variaron entre 29 y 275 kg ha-1; con mayores valores para el centeno. La producción de soja varió entre 1069 y 5346 kg ha-1 y no se afectó por la inclusión de CC. Estudios futuros deben, en el largo plazo, contemplar el beneficio de la incorporación de CC sobre las propiedades físicas y químicas del suelo en sistemas agrícolas de la región semiárida y subhúmeda pampeana dedicados al cultivo de soja. / The soil productivity can be limited because of a frequent presence of oil crops and because of their little residue cover production and persistence on the soils. In the semiarid and subhumid Pampas region, the use of winter cover crops (CC) could provide residues with high C contents and maintain an adequate soil residue cover. But, the soil water use of the CC during the winter season can modify the normal soil water availability for the follo-wing crop. The objective of this research was to evaluate the dry matter (MS) production efficiency of winter cereals cultivated as CC and their effect on the soil available water and soil nitrates under two soil moisture regimes. The study was performed in four experimental sites: Luan Toro (La Pampa) (Entic Haplustoll, mean anual rainfall of 624 mm), Dorila (La Pampa) (Entic Haplustoll, mean anual rainfall of 873 mm), General Villegas (Buenos Aires) (Thapto-argic Hapludoll, mean anual rainfall of 906 mm)and General Pinto (Buenos Aires) (Typic Argiudoll, mean anual rainfall of 1116 mm). The treatments were: Rye [Secale cereale (C)], Rye grass [Lolium multiflorum (R)] and oat [Avena sativa (A)] and an untreated control without CC (T). The MS production at the moment of killing the CC varied between 1226 and 10500 kg ha-1. A strong effect of the soil water regime on the MS production of the CC was observed. The soil water content at the moment of seeding the CC explained 57 % of the MS production and the soil water content at seeding and rainfalls during the CC growing season explained almost 89 % of the variability in MS production. In the four locations, rye showed the greater MS production followed by oat and rye grass. The greater variability was observed within each of the sites and the studied seasons. The water uptake at the moment of killing the CC varied between 88 and 299 mm. Rye was the cereal with greater water use efficiency with a highest value in General Pinto with 54 kg of MS mm-1. The soil penetration resistance (RP) values were higher under CC treatments and negatively related with the soil mois-ture contents. Only in Dorila the highest values of RP were observed in the control treatment without a significant relationship between them and the soil water contents. The soil N-NO3- contents were greater in the T than in the CC treatments. The N content of the CC biomass varied between 29 and 75 kg ha-1 achieving greater values with rye. The soybean grain production varied between 1069 and 5346 kg ha-1 without differences due to the CC treatments. Future studies should study the effects of the CC use of physical and chemical soil properties in agricultural systems from the semiarid and subhumid pampas region under continuous soybean crop production.

The purpose of this research carried out in 1998 and 1999 was to develop methods for strip cropping of cucumbers with rye/vetch and black plastic mulch. Effects of planting methods, weed control measures, and cover crop management techniques on pest and beneficial insects, petiole sap nitrate-nitrogen, soil moisture, yields and economic viability were assessed. Four treatments, namely cucumber direct seeded in black plastic mulch on tilled bare ground (conventional); cucumber direct seeded in black plastic mulch laid over incorporated rye/vetch residue; cucumber direct seeded into no-till rolled rye/vetch; and seedlings transplanted into no- till rolled rye/vetch, were evaluated in a randomized complete block design. Weeds were controlled in half the plot by critical manual weeding and pre-emergence herbicides applied in the other half in all treatments except the conventional system. Three-week interval staggered plantings of buckwheat to provide flowers for adult beneficial insects were also evaluated. Results obtained indicate that rye/vetch habitat is more attractive to beneficial insects than cucumber beetles before rolling or flail mowing. There were higher densities of adult Diptera (an indicator for Tachinid parasitoids) in both years and of Pennsylvania leatherwings in 1999 in plots with rye/vetch than in plots with rye only before planting. Preferential attraction to adult Diptera was not found after planting with no differences in cumulative densities between no-till and black plastic mulch plots after rolling or flail mowing. However, adult Diptera densities were positively correlated with cucumber yield, higher densities of cucumber beetles occurred in black plastic mulch than in no-till plots in both years, and bacterial wilt, transmitted by cucumber beetles, was reduced in no-till in 1999. Similarly, rye/vetch habitat plots had a higher diversity of Carabidae species (in both habitat areas and crop rows) before rolling or flail mowing, and higher densities of Carabidae (in habitat areas), Staphylinidae (in both habitat areas and crop rows) and spiders (in crop rows) after rolling or flail mowing. Black plastic mulch plots with flail mowed, incorporated rye/vetch residues had higher petiole sap nitrate-nitrogen and higher early season cucumber plant dry weights than in conventional plots; later in the season, the highest petiole sap nitrate-nitrogen occurred in no-till plots. No-till had higher marketable cucumber yield than plastic systems. The profitability of these production systems depended more on differences in marketable yield than on cost differences. The plastic with incorporated rye/vetch and no-till transplant systems were more profitable during early harvests in late July, while no- till direct seeded and transplant systems were more profitable from early August onwards. The three-week interval staggered planting of buckwheat led to conservation of beneficial insects, and the second and third seeded buckwheat flowering periods coincided with the period vetch had lost flowers. / Ph. D.

Field experiments were conducted in 1983 and 1984 to compare conventional tillage (CT) versus no-tillage (NT) production of broccoli and cabbage. The tillage treatments were applied in combination with four rates of applied nitrogen fertilizer and three cover crops: hairy vetch (<u>Vigia villosa</u> Roth), Austrian winter pea (<u>Fisum arvenu</u> L.), and cereal rye (<u>Secale cereale</u> L.). Transplants of 'Premium Crop' broccoli (<u>Brassica oleracea</u> var. <u>italica</u> Plenck) and 'Market Prize' cabbage (<u>Brassica oleracea</u> var. <u>capitata</u> L.) were set with a locally adapted NT vegetable planter. None of the variables studied affected the final plant stand. Yield and head size with NT were equal to or greater than CT. Soil moisture was higher under NT plots throughout the growing season. Yield and head size with the two legume covers were equal to or greater than those with the rye cover. Yield and head size tended to increase as applied N was increased, although differences were not always significant. The data, although not conclusive, indicate that the legume cover crops provided additional nitrogen for the cole crops. / Master of Science

The use of agrochemicals, like neonicotinoid insecticides can threaten human and environmental health when they are transported from agricultural fields. To minimize environmental impact of neonicotinoid pesticides to non-target organisms, it is important to quantify the movement of neonicotinoids from agricultural fields and examine how conventional agricultural practices can be altered using best management practices to minimize neonicotinoid transport. We developed a proportional runoff sampler that is inexpensive, rugged, and adaptable to existing runoff quantification systems. The sampler accurately collected flow-weighted samples under a broad range of steady-state and variable flow conditions. We then incorporated the sampler, along with leachate and soil sampling techniques, in a two-year field study testing the effects of winter cover crops and different edge-of-field buffer strip plant types on movement of the neonicotinoid thiamethoxam (TMX) and its metabolite clothianidin (CLO) in treated agricultural fields. Due to dry weather and other complications, runoff and leachate data could not be statistically analyzed. Soil samples indicated that cover crops had no effect on insecticide retention, so cover crops may not be a viable strategy to prevent neonicotinoid transport. Soil TMX was higher in grass buffers than native forb buffers in 2020; however, this result was not repeated in 2021 when vegetative cover was more consistent across treatments. CLO concentration did not vary by buffer in either year. Therefore, buffer strip plant type may have less impact on TMX and CLO retention than other factors like plant density. / Master of Science / Since the 1950's high yield crops and the use of pesticides and fertilizers have helped farmers increase yields from agricultural land. Increased yields have helped food production keep pace with population growth, but the use of pesticides and fertilizers can pose a threat to human and environmental health. Neonicotinoid insecticides can kill not only agricultural pests but beneficial invertebrates like bees and butterflies when the pesticides leave agricultural fields. To lessen environmental impact of neonicotinoid pesticides to the environment, it is important to understand 1) how these insecticides move from agricultural fields, and 2) how conventional agricultural practices can be altered to minimize neonicotinoid transport. We first developed a runoff sampler that was inexpensive, low maintenance, and adaptable to existing systems used to measure runoff. The sampler collects water at a rate proportional to the runoff rate under constant and changing flow rates. We then used the sampler along with leachate and soil sampling techniques in a field study testing the effect of winter cover crops and the species planted in edge-of-field vegetated buffer strips on the amount of the neonicotinoid thiamethoxam (TMX) and its degradation product clothianidin (CLO) remaining in the soil of treated fields during two growing seasons. Due to dry weather and other complications, runoff and leachate data could not be statistically analyzed. Cover crops did not change soil pesticide concentration either year, so cover crops may not be a viable strategy for preventing neonicotinoid transport. Soil TMX was higher in grass buffers than native forb buffers in 2020; however, this result was not detected in 2021 when plant growth in buffers was more uniform across treatments. CLO concentration did not vary by buffer type in either year. Therefore, the species planted in buffer strips may not be as important to holding TMX and CLO in soils as other factors like plant density.

Mid-Atlantic crop producers are increasingly transitioning to soil conservation methods that include reducing or eliminating tillage and planting high residue cover crops. These practices are associated with an increase in moderate to severe damage to field crops by slugs. Conserving, and even enhancing, natural enemy populations is a desirable way to manage slug infestations because remedial control measures are limited. To better understand how cover crop usage and tillage practices affect slug and natural enemy populations, 43 Virginia fields with different combinations of tillage practices and cover crop use were intensively sampled in 2018 and 2019. Fields were sampled over a six-week period during the early planting season when slugs are most problematic. Shingle traps and pitfall traps were used to sample slugs and natural enemies, respectively. To determine how multiple farming practices, soil composition, landscape features, and field history affect slug feeding injury to seedling plants, over 1,000 hectares of commercial production fields in the Shenandoah Region of Virginia were scouted for slug feeding injury to seedling plants. Corresponding crop producers were then surveyed on management methods. Our goal was to determine if slug feeding risk could be predicted by a single factor and or a combination of factors. Behavioral assays were performed with a common slug pest, Deroceras laeve, to determine if this species prefers feeding on maize, soybean, daikon radish, crimson clover, rye, or hairy vetch leaf tissue. Our sampling study found that cover crop use and conservation tillage type did not affect slug presence and damage, but that these factors affected various slug predators in different ways. We also observed that fields with more Phalangiidae and total predators overall had fewer slugs. Average slug feeding injury in both years was low and no factor or interaction of factors in our broader survey affected slug feeding injury ratings in fields. Behavioral assays indicated that slugs fed more on soybean tissue compared with maize, slugs consumed less maize when it was offered with hairy vetch or crimson clover, and slugs consumed less soybean when it was offered with hairy vetch or daikon radish. / Master of Science in Life Sciences / Invasive slug species the gray field slug, Deroceras reticulatum (Muller), and the marsh slug, Deroceras laeve, are major pests of maize and soybean grown in Virginia no-till systems. Slug feeding causes injury to young plants that, when severe, can reduce crop yield and necessitate replanting a field. Chemical control options are limited, and farmers may not be willing to use tillage as a control measure. Conserving arthropod slug predators may suppress pest populations below economic levels. Research is needed to understand how farming methods such as tillage type (e.g., no-till, strip tillage, vertical tillage) and cover crop use affect slugs and their predators. Our study investigated 1) how tillage type and cover crop use affected slug and slug predators, 2) if certain farming practices and field characteristics can be used to predict slug injury, and 3) if slugs preferred feeding on commonly-used cover crop species when offered with maize or soybean seedlings. Commercial production fields with different combinations of no tillage or reduced tillage, and planted with or without cover crops, were sampled to evaluate how these farming practices affect slugs and slug predators. Shingle traps and frames were used before planting and during early plant growth stages to collect and identify slug species; pitfall traps were used throughout the growing season to collect and identify slug predator species. To determine if slug feeding risk could be predicted, a survey of commercial production fields in the Shenandoah area was conducted and used to identify factors, if any, that influence slug feeding. Laboratory assays were used to determine feeding preference by offering slugs opportunity to feed on leaf tissue from a maize or soybean seedling or a mature cover crop species. We found that tillage type and use of cover crops did not affect the abundance of slugs, but that they did influence predator populations. The highest number of total predators were found in fields with reduced tillage, cover crops, and no insecticide use prior to crop emergence. Harvestmen were potentially the most impactful slug predator in the region. Field surveys suggested that no tested factor or interaction of factors affected slug injury to plants in commercial fields. Finally, we observed that slugs fed differently on soybean and maize tissue depending on cover crop species present.

Current Virginia nitrogen (N) fertilizer recommendations do not include site-specific estimates of N supply from cover crops (CCs) or soil organic matter (SOM). Recent research successfully predicted the contribution of N from SOM and CCs to corn (Zea mays L.) in Pennsylvania. The objective of this work was to validate the biophysical model developed in Pennsylvania under Virginia conditions and to evaluate the decomposition rates of different surface-applied CC residues and the relationship between their chemical composition and decomposition rate. For the first objective, 83 N response trials were conducted in different regions of Virginia across 9 years using a randomized complete block design with four replications. The model was able to explain 47% and 15% of variability in unfertilized corn yield (RMSE = 1.6 Mg ha-1) and economical optimum N rate (EONR) (RMSE = 30 kg N ha-1) respectively. Efforts to improve the model by adding economically unresponsive sites improved the model performance to explain 45% of the variability in EONR. For the second objective, a lab incubation was performed to compare carbon (C) and N mineralization from four different CCs {Cereal Rye (CR), Hairy vetch (HV), Crimson clover (Cc) and Rapeseed (R)} on a sandy loam soil. Destructive sampling was performed at 6 different sampling dates – 3, 7, 14, 28, 56 and 112 days. ANOVA test revealed that the effects of CC species, incubation days and their interaction had a significant effect on mass decomposed, plant biochemical composition and net N mineralization. Variation in mass loss was positively related to lignin content for all the CCs while it was moderately correlated to C:N ratio for CR and R and weakly to HV and Cc. Biomass loss and N release was highest in HV followed by Cc, R and CR. Net N mineralization was highest in HV followed by R, Cc and CR amended soils. / Master of Science / Current Virginia nitrogen (N) fertilizer recommendations do not include site-specific estimates of N supply from cover crops or soil organic matter, both of which can influence crop N need. Recent research successfully predicted the contribution of N from cover crops and soil to corn (Zea mays L.) in Pennsylvania. The objectives of this work were to validate the biophysical model developed in Pennsylvania under Virginia conditions and to evaluate the decomposition rates of different surface-applied cover crop residues and the relationship between their chemical composition and decomposition rate. The Pennsylvania-developed model was able to successfully estimate the economical optimum N rate for corn and predict the yield of unfertilized corn. Corn yield did not increase with increasing N rates in some fields. When these sites were omitted, the accuracy of the model improved. For the second objective, a lab incubation study was performed comparing C and N released from Cereal Rye (CR), Hairy vetch (HV), Crimson clover (Cc) and Rapeseed (R)} on a sandy loam soil. Destructive sampling was performed at 6 different sampling dates – 3, 7, 14, 28, 56 and 112 days. Variation in mass loss was positively related to lignin content for all the cover crops while it was moderately correlated to C:N ratio for CR and R and weakly to HV and Cc. Biomass loss and N release was highest in HV followed by Cc, R and CR.

Cover crops are known to produce many conservation benefits. However, grower adoption remains low due to various issues and poor economic returns. This study was conducted to identify factors and management practices needed to integrate cover crops into Midsouth corn production systems. The effects of cover crop species, herbicide termination timing, and mechanical termination methods on the growth, grain yields, and economic returns of corn [Zea Mays (L.)] were investigated in Mississippi. The presence of living cover crops at planting stunted corn growth when they were not terminated by herbicides at least four weeks preplant. A legume cover crop reduced corn yield only when it was terminated at planting. The inclusion of a legume benefited the system compared to cereal rye. Cover crops produced neutral to negative crop yields, and all treatments reduced economic returns. Negative economic returns and production risks will likely limit cover crop adoption by corn growers.

Supply and synchronization of plant-available nitrogen (N) to the soil is a major challenge for organic farmers, especially when growing crops in soils that are in transition from conventional to organic systems. This research evaluated the effects of site produced cover crops and application of soil amendments on N uptake and crop yield of organic Irish potato (Solanum tuberosum) and sweet corn (Zea mays). Cover crops were crimson clover (Trifolium incarnatum) and forage radish (Raphanus sativus). Soil amendments included in-row application of commercially produced dehydrated compost tea absorbed on charcoal (Soil Biology Innovations) and post-plant sidedressing with organic N fertilizer. Irish potato and sweet corn were grown at the Kentland Agricultural Research Farm near Blacksburg, VA in an organic transition soil during the summers of 2006 and 2007. Nitrogen uptake and crop yield were not affected by cover crop species in either year. SBI increased yield of sweet corn, but not Irish potato for both years; SBI had no effect on N uptake of either crop. Post-plant N sidedressing increased N uptake and crop yield of Irish potato and sweet corn in 2007, but had no effect on crop yield in 2006, presumably because pre-plant organic fertilizer was applied at planting in 2006, but not in 2007. This study shows that the combination of site produced cover crops and applied soil amendments may be required to produce high marketable yields of organic Irish potato and sweet corn in the transition soil used in these experiments. / Master of Science