Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2015-10-08T22:13:49Z No. of bitstreams: 1 Disserta??o Final_Fabio Ribeiro Garcia.pdf: 1235311 bytes, checksum: 99c4c15b4044b2e3d060b0f7f4d595d4 (MD5) / Made available in DSpace on 2015-10-08T22:13:49Z (GMT). No. of bitstreams: 1 Disserta??o Final_Fabio Ribeiro Garcia.pdf: 1235311 bytes, checksum: 99c4c15b4044b2e3d060b0f7f4d595d4 (MD5) Previous issue date: 2013-05-07 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / bromeliad species are native to Brazil and have great potential for use as an ornamental plant, and a relevant ecological importance. Considering the devastation of the Atlantic, currently 7.3% of its original area preserved and where about 70% are endemic bromeliad is important to establish methods of propagation and ex situ conservation in order to preserve this germplasm and avoid irreversible genetic erosion. Thus, the aim of this work was to establish protocols and micropropagation in vitro conservation of A. blancheteana and A. miniata. In the first chapter, for multiplication in vitro, we used different concentrations of BAP and for rooting tested different concentrations of auxins IAA, IBA and NAA. In the second chapter we tested two protocols, was first used in MS medium supplemented with different concentrations of auxin Picloram and 2,4-D combined with BAP and the second experiment evaluated MS medium supplemented with 2,4-D or picloram in different concentrations. In the third chapter, we investigated the effect of osmotic agents, sucrose, sorbitol and mannitol in vitro conservation A. blancheteana. For the best result micropropagation for multiplication was for medium supplemented with 4.44 ?M BA. To induce somatic embryos and regeneration of the concentration of 22.5 ?M 2,4-D was the most efficient. Independent of concentration, mannitol was more efficient for in vitro conservation of A. blancheteana for 12 months. / Aechmea blancheteana (Baker) L.B. Smith e Aechmea miniata (Beer) Baker s?o esp?cies de brom?lias nativas do Brasil que possuem grande potencial para uso como planta ornamental, al?m de relevante import?ncia ecol?gica. Considerando a grande devasta??o de Mata Atl?ntica, atualmente com 7,3% de sua ?rea original preservada, onde cerca de 70% das brom?lias s?o end?micas ? importante o estabelecimento de m?todos propaga??o e conserva??o ex situ com o objetivo de preservar esse germoplasma e evitar uma eros?o gen?tica irrevers?vel. Assim, o objetivo deste trabalho estabelecer os protocolos de micropropaga??o e conserva??o in vitro de A. blancheteana e A. miniata. No primeiro cap?tulo, para a multiplica??o in vitro, foram utilizadas diferentes concentra??es de BAP e na fase de enraizamento foram testadas diferentes concentra??es das auxinas AIA, AIB e ANA. No segundo cap?tulo foram testadas a combina??o de diferentes concentra??es das auxinas Picloram e 2,4-D e da auxina 2,4-D combinada com a citocinina BAP na indu??o de embri?es som?ticos. No terceiro cap?tulo, foi avaliado o efeito dos agentes osm?ticos, sacarose, sorbitol e manitol na conserva??o in vitro de A. blancheteana. Para a micropropaga??o o melhor resultado foi obtido em meio MS suplementado com 4,44?M de BAP. Para a indu??o e regenera??o de embri?es som?ticos, a concentra??o 22,5?M de 2,4-D foi a mais eficiente. Independente da concentra??o, o manitol foi mais eficiente para a conserva??o in vitro de A. blancheteana por 12 meses.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-07-19T23:28:56Z No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) / Made available in DSpace on 2016-07-19T23:28:56Z (GMT). No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) Previous issue date: 2016-03-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Chapada Diamantina ? BA presents endemic especies with ornamental potential among which is Orthophytum mucugense, which occurs in the municipality Mucug?. This species is a target of extractivism and it is considered vulnerable, rendering studies of propagation and conservation of the species necessary. Therefore, the objective of this study was to establish protocols of micropropagation and in vitro conservation of O. mucugense. To that end, morphogenetic responses were evaluated in three types of explants, stem, root and leaf under the effect of different concentrations of the plant growth regulators NAA, 2,4-D and BAP. Sprouts formation in stem explants by direct organogenesis, in basal leaf explants by indirect organogenesis and callus formation in basal root and leaf explants were obtained. The rooting of the shoots was carried out with 1 coal g.L-1 activated for 30 days, and microplants acclimatized in a greenhouse with direct exposure to the environment. These results evince that tissue culture is a viable tool for in vitro propagation of O. mucugense. In in vitro conservation the effect of the medium salts reduction, of osmotic agents and of the retardant ancymidol in the minimum growth of the plants were tested; after 300 days of cultivation, analysis of the plant growth, of the chlorophyll content and of the regenerative capacity were carried out. On the basis of these assessments, the ancymidol, in the concentrations used, is not efficient in minimal growth induction, and the reduction of MS salts to ?3, with the combination of 45 g.L-1 of sucrose with 7.8 g.L-1 of mannitol is indicated for in vitro conservation of O. mucugense. / A Chapada Diamantina ? BA apresenta esp?cies end?micas com potencial ornamental dentre as quais est? a Orthophytum mucugense, que ocorre no munic?pio de Mucug?. Esta esp?cie ? alvo de extrativismo e considerada como vulner?vel, sendo necess?rios estudos de propaga??o e conserva??o da mesma. Portanto, objetivou-se neste trabalho estabelecer protocolos de micropropaga??o e conserva??o in vitro de O. mucugense. Para isto foram avaliadas as respostas morfog?nicas em tr?s tipos de explantes, caulinar, radicular e foliar, sob efeito de diferentes concentra??es dos reguladores vegetais ANA, 2,4-D e BAP. Foram obtidas forma??o de brotos em explante caulinar por organog?nese direta, em explante foliar basal por organog?nese indireta e forma??o de calo em explante foliar basal e radicular. O enraizamento dos brotos foi realizado com 1 g.L-1 de carv?o ativado por 30 dias, e as microplantas aclimatizadas em casa de vegeta??o com exposi??o direta ao ambiente. Estes dados demonstram que a cultura de tecidos ? uma ferramenta vi?vel para a propaga??o in vitro de O. mucugense. Na conserva??o in vitro foram testados o efeito da redu??o de sais do meio, de agentes osm?ticos e do retardante ancymidol no crescimento m?nimo das plantas; ap?s 300 dias de cultivo foram realizadas an?lises de crescimento das mesmas, do teor de clorofila e da capacidade regenerativa. Com base nestas avalia??es o ancymidol, nas concentra??es utilizadas, n?o ? eficiente na indu??o do crescimento m?nimo, e a redu??o de sais MS para ?3 com a combina??o de 45 g.L-1 de sacarose com 7,8 g.L-1 de manitol ? indicada para conserva??o in vitro de O. mucugense.