Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-04-11T21:06:17Z No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) / Made available in DSpace on 2017-04-11T21:06:17Z (GMT). No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) Previous issue date: 2016-09-26 / (In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase. / Alpinia zerumbet e Solidago chilensis s?o conhecidas pelos valores ornamental e medicinal. Existem poucos relatos na literatura sobre micropropaga??o de Alpinia zerumbet e nenhum sobre Solidago chilensis, o que demonstra necessidade de estudos. Este trabalho objetivou estudar a propaga??o in vitro das esp?cies, envolvendo micropropaga??o e desenvolvendo protocolos para organog?nese. Para o estabelecimento in vitro foram testados diferentes tipos de explantes, m?todos de desinfesta??o e antioxidantes. Para multiplica??o, esses explantes foram testados com reguladores vegetais: ?cido naftalenoac?tico e benzilaminopurina isolados e combinados, al?m de ?cido 2,4-diclorofenoxiac?tico e cinetina, isolados e combinados. Foi feita caracteriza??o anat?mica da calog?nese. Para aclimatiza??o, ap?s a pr?-aclimatiza??o, as mudas foram transferidas para copos pl?sticos contendo substrato esterilizado, tampados com garrafas pet e levados para casa de vegeta??o com sombrite 50%, onde as tampas das garrafas foram desenroscadas aos poucos. Os resultados mostraram sucesso no estabelecimento in vitro de A. zerumbet, etapa crucial para iniciar cultivo em larga escala. Contra contamina??o, o tratamento mais efetivo foi 4mL de PPM/L (Plant Preservative Mixture), controlando 100% dos pat?genos. Como antioxidante, o ?cido asc?rbico (2%) foi o mais eficiente. Houve brota??o de A. zerumbet em explantes derivados de gemas. N?o foi observado decl?nio na taxa de propaga??o no decorrer dos subcultivos in vitro, contudo o crescimento ? muito lento. Para S. chilensis, explantes de segmentos nodais apresentaram maior taxa de multiplica??o. N?o foi observado decl?nio na taxa de propaga??o no decorrer de quatro subcultivos in vitro e as mudas apresentaram alto ?ndice de sobreviv?ncia na fase de aclimatiza??o.

Submitted by Jadson Francisco de Jesus SILVA (jadson@uefs.br) on 2018-01-30T22:51:06Z No. of bitstreams: 1 Tese Definitivo ZERGurgel.pdf: 2392487 bytes, checksum: 97870ae444449d037535f874c18b1d37 (MD5) / Made available in DSpace on 2018-01-30T22:51:06Z (GMT). No. of bitstreams: 1 Tese Definitivo ZERGurgel.pdf: 2392487 bytes, checksum: 97870ae444449d037535f874c18b1d37 (MD5) Previous issue date: 2017-07-24 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Comanthera mucugensis Giul. subsp. mucugensis, an endemic species of the municipality of Mucug?-BA is threatened with extinction. To reduce extractivism in natural populations, it is necessary to develop efficient multiplication protocols; In this sense somatic embryogenesis and organogenesis may be viable and preservation cryopreservation may be a strategy for its long-term. The objectives of this study were: (1) to analyze the embryogenic competence and the indirect organogenesis of C. mucugensis; (2) to evaluate the cryopreservation of seeds and different methods of cryopreservation of C. mucugensis plants and (3) to identify the best way to store seeds in the long term. Experiments were performed to induce somatic embryogenesis with different concentrations of 2,4-D X BAP and Picloran X BAP and for indirect organogenesis BAP and ANA were used. Seeds were cryopreserved for 0 (control), 1, 7, 30, 360 and 540 days and other seeds were stored at different temperatures to verify the best form of storage. Plants were cryopreserved with the technique of vitrification and encapsulation-desiccation. Plants from cryopreserved seeds were acclimatized in sand and vegetal soil (1: 1) for 60 days. The work showed that the plant regulators Picloran and 2,4-D are promising in the induction of callus with embryogenic potential and that the plant regulator ANA at 4.9 ?M is efficient in indirect organogenesis. The seeds of C. mucugensis can be cryopreserved without compromising their physiological quality, but the techniques for cryopreservation of plants have not been efficient. / Comanthera mucugensis Giul. subsp. mucugensis, possui flor de interesse comercial que devido ao extrativismo excessivo encontra-se amea?ada de extin??o. Para suprir a demanda do mercado e evitar o decl?nio populacional, faz-se necess?rio desenvolver protocolos para sua multiplica??o, podendo a embriog?nese som?tica e a organog?nese serem alternativas vi?veis. Al?m disso, ? importante investir em estudos de conserva??o a longo prazo como, por exemplo, a criopreserva??o. O trabalho teve como objetivos: realizar estudos para tornar a micropropaga??o mais eficiente e avaliar a conserva??o de C. mucugensis em diferentes temperaturas como estrat?gia para a conserva??o do seu germoplasma. Foram testados para a indu??o da embriog?nese som?tica 2,4-D x BAP e Picloram x BAP e para organog?nese indireta BAP e ANA. Na criopreserva??o foram avaliadas sementes mantidas em nitrog?nio l?quido (-196?C) por 0, 1, 7, 30, 360 e 540 dias e as plantas inteiras foram submetidas a duas t?cnicas: vitrifica??o e encapsulamento-desidrata??o. Para avaliar o armazenamento, as sementes foram mantidas em temperatura ambiente, 4?C, -20?C, -80?C e -196?C por 30, 90 e 180 dias. Visando observar se a criopreserva??o das sementes interfere no desenvolvimento, plantas oriundas da germina??o in vitro de sementes criopreservadas foram aclimatizadas em areia e terra vegetal (1:1) por 60 dias. O trabalho demonstrou que os reguladores vegetais Picloram e 2,4-D s?o promissores na indu??o de calos com potencial embriog?nico e que o regulador vegetal ANA (4,9 ?M) ? eficiente na organog?nese indireta. As sementes de C. mucugensis podem ser criopreservadas sem comprometer sua qualidade fisiol?gica, entretanto, n?o foram eficientes as t?cnicas para criopreserva??o de plantas inteiras.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-07-19T23:28:56Z No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) / Made available in DSpace on 2016-07-19T23:28:56Z (GMT). No. of bitstreams: 1 DISSERTA??O ANDRESSA PRISCILA PIANC? S. LIMA.pdf: 1903052 bytes, checksum: 3d8a27256e27d81bbdd1cf3ad9eee48f (MD5) Previous issue date: 2016-03-23 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Chapada Diamantina ? BA presents endemic especies with ornamental potential among which is Orthophytum mucugense, which occurs in the municipality Mucug?. This species is a target of extractivism and it is considered vulnerable, rendering studies of propagation and conservation of the species necessary. Therefore, the objective of this study was to establish protocols of micropropagation and in vitro conservation of O. mucugense. To that end, morphogenetic responses were evaluated in three types of explants, stem, root and leaf under the effect of different concentrations of the plant growth regulators NAA, 2,4-D and BAP. Sprouts formation in stem explants by direct organogenesis, in basal leaf explants by indirect organogenesis and callus formation in basal root and leaf explants were obtained. The rooting of the shoots was carried out with 1 coal g.L-1 activated for 30 days, and microplants acclimatized in a greenhouse with direct exposure to the environment. These results evince that tissue culture is a viable tool for in vitro propagation of O. mucugense. In in vitro conservation the effect of the medium salts reduction, of osmotic agents and of the retardant ancymidol in the minimum growth of the plants were tested; after 300 days of cultivation, analysis of the plant growth, of the chlorophyll content and of the regenerative capacity were carried out. On the basis of these assessments, the ancymidol, in the concentrations used, is not efficient in minimal growth induction, and the reduction of MS salts to ?3, with the combination of 45 g.L-1 of sucrose with 7.8 g.L-1 of mannitol is indicated for in vitro conservation of O. mucugense. / A Chapada Diamantina ? BA apresenta esp?cies end?micas com potencial ornamental dentre as quais est? a Orthophytum mucugense, que ocorre no munic?pio de Mucug?. Esta esp?cie ? alvo de extrativismo e considerada como vulner?vel, sendo necess?rios estudos de propaga??o e conserva??o da mesma. Portanto, objetivou-se neste trabalho estabelecer protocolos de micropropaga??o e conserva??o in vitro de O. mucugense. Para isto foram avaliadas as respostas morfog?nicas em tr?s tipos de explantes, caulinar, radicular e foliar, sob efeito de diferentes concentra??es dos reguladores vegetais ANA, 2,4-D e BAP. Foram obtidas forma??o de brotos em explante caulinar por organog?nese direta, em explante foliar basal por organog?nese indireta e forma??o de calo em explante foliar basal e radicular. O enraizamento dos brotos foi realizado com 1 g.L-1 de carv?o ativado por 30 dias, e as microplantas aclimatizadas em casa de vegeta??o com exposi??o direta ao ambiente. Estes dados demonstram que a cultura de tecidos ? uma ferramenta vi?vel para a propaga??o in vitro de O. mucugense. Na conserva??o in vitro foram testados o efeito da redu??o de sais do meio, de agentes osm?ticos e do retardante ancymidol no crescimento m?nimo das plantas; ap?s 300 dias de cultivo foram realizadas an?lises de crescimento das mesmas, do teor de clorofila e da capacidade regenerativa. Com base nestas avalia??es o ancymidol, nas concentra??es utilizadas, n?o ? eficiente na indu??o do crescimento m?nimo, e a redu??o de sais MS para ?3 com a combina??o de 45 g.L-1 de sacarose com 7,8 g.L-1 de manitol ? indicada para conserva??o in vitro de O. mucugense.

Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2016-08-24T20:52:09Z No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) / Made available in DSpace on 2016-08-24T20:52:09Z (GMT). No. of bitstreams: 1 Disserta??o Final_Fl?via_PPGRGV (2).pdf: 1857571 bytes, checksum: 967f2b7d728622a11a98a803f13ff0de (MD5) Previous issue date: 2015-03-20 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / Hyptis ramosa Pohl ex Benth (Lamiaceae) is native and endemic to the semi-arid northeast, with its unknown phytochemical constitution so far. Considering the pharmacological importance of the species of this family, the development of forms of propagation and in vitro culture can contribute to the inclusion of these species in sustainable production systems and the conservation of the same. Thus, the objective of this work was to study the in vitro propagation of the species H. ramosa, through direct organogenesis and callus formation, and the biochemical characterization of the obtained calluses, thus allowing the establishment of strategies for their conservation and sustainable use. To this, H. ramosa seeds were disinfected and established in medium MS / 2 culture. In the multiplication phase was tested the influence of cytokinins BAP, CIN and TDZ on different explants. The obtained shoots were individualized and transferred to MS / 2 medium containing different concentrations of auxin IBA and activated carbon for rooting them. Regenerated and rooted in vitro microplants were subjected to pre-acclimatization in different cultivation container closure and then were transferred to ex vitro conditions in commercial substrate Plantmax? being quantified plant survival rate at 30 days after transfer. For callus induction, we used explants and different concentrations of 2,4-D and BAP, determining the growth curve from the fresh weight of callus until the 28th day of cultivation, at intervals of seven days. Concurrent with obtaining the growth curve it is quantified in calluses obtained the total soluble sugar content, reducing sugar and crude protein. The in vitro propagation of H. ramosa is possible using the nodal segment as a source of explants in MS medium supplemented with BAP. In vitro rooting occurs, even in free auxin. The species showed survival of 100%, regardless of the day of pre-acclimatization phase. For callus induction the best explant is the nodal segment, and the combination of 2.4-D and BAP favor the formation of the same. The callus growth curve showed quadratic behavior with two different phases and biochemical analysis showed the maximum level of total soluble sugars, reducing sugars and crude protein at 14 ?, 21 ? and 14 ? days, respectively. / Hyptis ramosa Pohl ex Benth (Lamiaceae) ? uma esp?cie nativa e end?mica do semi?rido nordestino, sendo sua constitui??o fitoqu?mica desconhecida at? o momento. Considerando a import?ncia farmacol?gica das esp?cies dessa fam?lia, o desenvolvimento de formas de propaga??o e cultivo in vitro poder? contribuir para a inser??o dessas esp?cies em sistemas de produ??o sustent?veis e a conserva??o das mesmas. Diante disso, o objetivo deste trabalho foi estudar a propaga??o in vitro da esp?cie H. ramosa, atrav?s de organog?nese direta e calog?nese, bem como a caracteriza??o bioqu?mica dos calos obtidos, permitindo assim o estabelecimento de estrat?gias para a sua conserva??o e explora??o sustent?vel. Para isso, sementes de H. ramosa foram desinfestadas e estabelecidas em meio de cultura MS/2. Na fase de multiplica??o foi testada a influ?ncia das citocininas BAP, CIN e TDZ sobre diferentes explantes. As brota??es obtidas foram individualizadas e transferidas para meio MS/2 contendo diferentes concentra??es da auxina AIB e de carv?o ativo para o enraizamento das mesmas. As microplantas regeneradas e enraizadas in vitro foram submetidas ? pr?-aclimatiza??o em diferentes tipos de fechamento do recipiente de cultivo e, posteriormente, foram transferidas para a condi??o ex vitro em substrato comercial Plantmax?, sendo quantificada a taxa de sobreviv?ncia das plantas aos 30 dias ap?s a transfer?ncia. Para a indu??o de calos utilizou-se diferentes explantes e concentra??es de 2,4-D e BAP, determinando-se a curva de crescimento a partir da mat?ria fresca dos calos at? o 28o dia de cultivo, em intervalos de sete dias. Concomitante com a obten??o da curva de crescimento quantificou-se nos calos obtidos o teor de a??cares sol?veis totais, a??cares redutores e prote?na bruta. A propaga??o in vitro de H. ramosa ? poss?vel utilizando-se o segmento nodal como fonte de explante, em meio de cultura MS suplementado com BAP. O enraizamento in vitro ocorre, mesmo em meio isento de auxina. A esp?cie apresentou sobreviv?ncia de 100%, independentemente da realiza??o da fase de pr?-aclimatiza??o. Para indu??o de calos o melhor explante ? o segmento nodal, sendo a combina??o de 2.4-D e BAP favor?vel a forma??o dos mesmos. A curva de crescimento de calos mostrou comportamento quadr?tico com duas fases distintas e a an?lise bioqu?mica evidenciou o teor m?ximo de a??cares sol?veis totais, a??cares redutores e prote?na bruta aos 14?, 21? e 14? dias, respectivamente.