Submitted by Ricardo Cedraz Duque Moliterno (ricardo.moliterno@uefs.br) on 2017-04-11T21:06:17Z No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) / Made available in DSpace on 2017-04-11T21:06:17Z (GMT). No. of bitstreams: 1 Tese final Ana Carolina-.pdf: 2069305 bytes, checksum: d639d8880ea9572c9a3ab7a9e940b690 (MD5) Previous issue date: 2016-09-26 / (In vitro propagation and acclimatization of medicinal species: Alpinia zerumbet (Pers.) B.L.Burtt&R.M.Sm. (Zingiberaceae) and Solidago chilensis Meyen (Asteraceae). Alpinia zerumbet and Solidago chilensis are known for their ornamental and medicinal values. There are few reports in the literature on micropropagation of Alpinia zerumbet and none about Solidago chilensis, which demonstrates the need for studies. This work aimed to study in vitro propagation of the species, involving micropropagation and developing protocols for organogenesis. For in vitro establishment were tested different types of explants, disinfestation methods and antioxidants. For multiplication, these explants were tested with plant growth regulators naftalen acetic acid and benzilaminopurin isolated and combined, and dichlorophenoxyacetic acid and kinetin, isolated and combined. It was made anatomical characterization of callus formation. For acclimatization, after pre-acclimatization, the seedlings were transferred to plastic cups containing sterilized substrate, capped with plastic bottles and taken to a greenhouse with 50% shading, where the bottle caps were unscrewed slowly. The results showed success in establishing in vitro of A. zerumbet crucial step to start large-scale cultivation. Against contamination, the most effective treatment was 4ml PPM/L (Plant Preservative Mixture), controlling 100% of pathogens. As an antioxidant, ascorbic acid (2%) was the most efficient. There was budding A. zerumbet derivatives bud explants. There was no decline in the propagation rate during in vitro subcultures, however growth is very slow. S. chilensis, explants nodal segments showed a higher rate of multiplication. There was no decline in the spread rate over four subcultures in vitro and the seedlings had a high survival rate in the acclimatization phase. / Alpinia zerumbet e Solidago chilensis s?o conhecidas pelos valores ornamental e medicinal. Existem poucos relatos na literatura sobre micropropaga??o de Alpinia zerumbet e nenhum sobre Solidago chilensis, o que demonstra necessidade de estudos. Este trabalho objetivou estudar a propaga??o in vitro das esp?cies, envolvendo micropropaga??o e desenvolvendo protocolos para organog?nese. Para o estabelecimento in vitro foram testados diferentes tipos de explantes, m?todos de desinfesta??o e antioxidantes. Para multiplica??o, esses explantes foram testados com reguladores vegetais: ?cido naftalenoac?tico e benzilaminopurina isolados e combinados, al?m de ?cido 2,4-diclorofenoxiac?tico e cinetina, isolados e combinados. Foi feita caracteriza??o anat?mica da calog?nese. Para aclimatiza??o, ap?s a pr?-aclimatiza??o, as mudas foram transferidas para copos pl?sticos contendo substrato esterilizado, tampados com garrafas pet e levados para casa de vegeta??o com sombrite 50%, onde as tampas das garrafas foram desenroscadas aos poucos. Os resultados mostraram sucesso no estabelecimento in vitro de A. zerumbet, etapa crucial para iniciar cultivo em larga escala. Contra contamina??o, o tratamento mais efetivo foi 4mL de PPM/L (Plant Preservative Mixture), controlando 100% dos pat?genos. Como antioxidante, o ?cido asc?rbico (2%) foi o mais eficiente. Houve brota??o de A. zerumbet em explantes derivados de gemas. N?o foi observado decl?nio na taxa de propaga??o no decorrer dos subcultivos in vitro, contudo o crescimento ? muito lento. Para S. chilensis, explantes de segmentos nodais apresentaram maior taxa de multiplica??o. N?o foi observado decl?nio na taxa de propaga??o no decorrer de quatro subcultivos in vitro e as mudas apresentaram alto ?ndice de sobreviv?ncia na fase de aclimatiza??o.

Submitted by Verena Bastos (verena@uefs.br) on 2015-10-14T21:06:54Z No. of bitstreams: 1 Paloma_R._Meira-disserta??o_2013.pdf: 1508066 bytes, checksum: cc4d4d39667159152b8407fa887ad2fa (MD5) / Made available in DSpace on 2015-10-14T21:06:54Z (GMT). No. of bitstreams: 1 Paloma_R._Meira-disserta??o_2013.pdf: 1508066 bytes, checksum: cc4d4d39667159152b8407fa887ad2fa (MD5) Previous issue date: 2013-09-19 / Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior - CAPES / This paper describes the influence of abiotic and biotic factors in the concentration of secondary metabolites of pharmaceutical interest in medicinal plants, while it aims to answer how these factors influence the concentration of podophyllotoxin and yate?na Leptohyptis macrostachys in vitro, this goal. The specimen Leptohyptis macrostachys was established in vitro from seeds collected in the Chapada Diamantina-Ba; studying the rate of germination rate, the germination and mean germination of seeds grown on MS medium with different concentrations of salts constituents of this medium as well as different concentrations of sucrose and gibberellic acid. Was also studied, such as abiotic factors (culture medium, carbon source temperature, photoperiod and gas exchange) and biological (plant growth regulators) influenced the development (number of shoots and leaves and roots presence of friable callus, compact or oxidized) and production of podophyllotoxin and yate?na. To study the influence of these factors in getting these metabolites, plants were steeped at 40 ? C in ethanol to produce the extract. This was partitioned with water and after a specific treatment, injected into the HPLC. We established a protocol for Leptohyptis macrostachys growing from seeds in ? MS medium supplemented with 1.5% sucrose and 11.55 mM of GA3, there was no significant formation of callus in treatments applied and the concentration of podophyllotoxin was favored when L. macrostachys was grown in ? MS medium supplemented with 1.5% sucrose, closed with PVC film and kept in a growth room at 30 ? C. / Este trabalho descreve a influ?ncia de fatores abi?ticos e bi?tico na concentra??o de metab?litos secund?rios de interesse farmacol?gico em plantas medicinais, ao mesmo tempo que se prop?e a responder como esses fatores influenciam na concentra??o de podofilotoxina e yate?na em Leptohyptis macrostachys in vitro, objetivo deste. A esp?cime Leptohyptis macrostachys foi estabelecida in vitro a partir de sementes coletadas na regi?o da Chapada Diamantina-Ba; estudando o ?ndice de velocidade de germina??o, a germinabilidade e o tempo m?dio de germina??o de sementes cultivadas em meio MS com diferentes concentra??es de sais constituintes deste meio, bem como diferentes concentra??es de sacarose e ?cido giber?lico. Foi estudado tamb?m, como fatores abi?ticos (meio de cultivo, fontes de carbono temperatura, fotoper?odo e trocas gasosas)e bi?tico (reguladores vegetais) influenciaram no desenvolvimento (quantidade de brotos folhas e ra?zes e presen?a de calos fri?veis, compactos ou oxidados) e na produ??o de podofilotoxina e yate?na. Para estudar a influ?ncia estes fatores na obten??o destes metab?litos, as plantas foram maceradas ? 40?C em etanol para a produ??o do extrato. Este foi particionado com ?gua e, ap?s tratamento espec?fico, injetado no CLAE. Foi estabelecido um protocolo de cultivo para Leptohyptis macrostachys a partir de sementes em meio MS ? suplementado com 1,5% de sacarose e 11,55?M de GA3; n?o houve forma??o significativa de calos nos tratamentos aplicados e a concentra??o da podofilotoxina foi favorecida quando L. macrostachys foi cultivada em meio MS ? suplementado com 1,5% de sacarose, fechada com filme de PVC e mantida em sala de crescimento a 30?C.