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Optimizing riboflavin/ultraviolet-a corneal collagen cross-linking for the treatment of progressive keratoconusSylvestre, Daniel Joseph 13 July 2017 (has links)
Patients with keratoconus exhibit a biomechanically weakened cornea which loses its proper shape and thereby loses its refractive power. It is usually progressive, beginning with poor visual acuity and eventually necessitating corneal transplant. The cause is likely multifactorial, but involves the weakening of the collagen structure of the corneal stroma, resulting in characteristic thinning and conical distortion. Collagen cross-linking is the first treatment to demonstrate efficacy in halting the progression of the disease. UVA radiation is used to activate riboflavin and photochemically induce cross-linking reactions among collagen and proteoglycans within the stroma, thereby stiffening and strengthening the tissue, and preventing further loss of shape. The current standard treatment, which gained FDA approval less than one year ago, has proven to be efficacious, but has been modified very little since pioneering experiments. Optimization aims to maximize clinical effect while maintaining safety and reducing total treatment time. Major procedural modifications involve increasing light intensity over a reduced exposure duration, and varying the method of delivering riboflavin to the stroma. Theoretical modeling, informed by and scaled to experimental results, has the potential to predict clinical effect as a function of treatment parameters, enabling tailoring of individual treatments to the specific needs of each patient.
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New insights into the structure and assembly of nuclear lamins from chemical cross-linking and mass spectrometryMakarov, Alexandr January 2017 (has links)
Now that the functioning of microtubules and the actin cytoskeleton has been worked out in enormous detail, the next important task is defining the structure of intermediate filaments that are far behind the other two major skeletal networks due to their inherent resistance to most structural techniques. The evolution of novel structural approaches for flexible proteins is making this possible now. In my thesis I will aim to elucidate the structure and assembly principles of lamin A nuclear intermediate filament protein. To study lamin A, I principally employed chemical cross-linking that allows the capturing of full-length protein structures in solution. I combined this with mass spectrometry approaches to identify cross-linked residues at the various stages of lamin A assembly that were additionally tracked with SILAC labelling and rotary metal shadowing TEM. Unlike previous cross-linking studies on intermediate filaments I use a zero-length self-excluding cross-linking agent EDC that is better tailored for investigation of the polar interactions between multiple unstructured or otherwise flexible charged sequences of lamins. Using this composite approach I interrogated lamin A dimeric and tetrameric assemblies. I elucidated hinge-like properties of the L12 and found indications that L1 and the region containing coil 2A and L2 and the beginning of coil 2B possess properties of linker-like flexibility and of predicted linear α-helical bundle and could act as molecular springs or compression buffers for the nuclear intermediate filaments. Further I confirm the role of the N-terminal unstructured region in lamin A assembly and for the first time show similar role for the C-terminal unstructured region flanking the rod domain of lamin A. Collected data strongly supports the model where both positively charged unstructured regions participate in extensive interaction with acidic rod termini and act as molecular bridges between these in the head-to-tail interface, confirming the uniformity of this principle between cytoplasmic and nuclear intermediate filaments. Formation of these bridges requires conformational change likely happening due to proline residues in the mitotic phosphorylation sites. Finally I suggest a mechanism of regulation of the order of assembly unique to the nuclear intermediate filament where C-terminal unstructured region blocks lateral interactions until it is tethered to the head-to-tail interface. Collected data on the dynamic behaviour of the C-terminal unstructured region and its ability to tether lamin A Ig domain may have far reaching implications for filament assembly and regulation of binding of hundreds of lamin A partner proteins presenting an important step in our understanding of relationship between lamin A structure and function and how altering the former could lead to disease.
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Estudo da matriz orgânica dentinária modificada por agentes naturais ricos em proantocianidina e seu uso para aumentar a efetividade de restaurações adesivas / Study of the dentin organic matrix modified by proanthocyanidin rich agents and their use to increase short and long-term bond strengthCastellan, Carina Strano 09 December 2010 (has links)
O objetivo deste trabalho foi elucidar a interação entre a proantocianidina (PA), um agente natural e não tóxico de ligação cruzada, e o colágeno dentinário tipo I. A hipótese proposta é que o modelamento de uma dentina desmineralizada mais resistente e com melhores propriedades propiciará um substrato mais estável e íntegro para restaurações adesivas. Para isto, o estudo foi dividido em 4 fases: 1) Validação da PA como agente exógeno de ligação cruzada, testando matrizes de dentina desmineralizadas tratadas com extratos a base de PA em teste microflexural para análise do módulo de elasticidade (ME), variando o tempo de exposição (10, 30, 60, 120, 240 min), o tipo de extrato usado (semente de uva-GSE, semente de cacau-CSE, oxicoco-CRE, canela-CNE e açaí-ACE) e a longevidade (imediato, 3, 6 e 12 meses); 2) Estudar parâmetros relacionados às soluções de PA, como solubilidade (água, etanol e acetona), fonte (GSE e CSE) e concentração (0,65%, 3,25%, 6,5%, 15% e 30%), através da mensuração do ME pelo teste micro-flexural de matrizes de dentina desmineralizada; 3) Caracterização da dentina tratada com PA (GSE e CSE), sorção de água, calorimetria e degradação enzimática; 4) Analisar a resistência de união entre a dentina tratada com PA (GSE/CSE) e sistemas de adesivos comerciais (Adapter Single Bond Plus e One Step Plus), variando a concentração (6,5% e 30%), tempo de exposição (1, 10 e 60 min) e longevidade (imediato, 3, 6 e 12 meses). Foram realizadas análises de variâncias e teste de contraste de médias, quando necessário, para todos os ensaios. Os resultados de ME foram influenciados pelo tratamento com PA, dependendo do tipo de extrato usado, exibindo os melhores resultados para o maior tempo de exposição (240 min) para os extratos GSE e CSE. Embora os resultados imediatos com os extratos CRE e CNE não tenham aumentado, estes se mantiveram constantes apos 1 ano de armazenamento, o que não aconteceu para o ACE e os grupos controles. A solubilidade das soluções é influenciada diretamente pelo modo de extração e manufaturação dos extratos, sendo que GSE é solúvel em água destilada e CSE em acetona-água. A efetividade do GSE mostrou ser concentração-dependente, exibindo o maior ME para a concentração de 30%. A dentina tratada com agentes a base de PA foi mais resistente contra a colagenase, obteve uma menor sorção de água e um aumento significativo na temperatura de desnaturação quando comparada ao grupo controle. O GSE foi capaz de aumentar a resistência de união (RU) imediata para ambos os sistemas adesivos, mantendo-se constante para o Adapter Single Bond Plus ao longo do tempo. Para o One Step Plus todos os grupos mostraram diminuição da RU após 1 ano, independente do tempo de exposição. Quando a dentina foi tratada por 1 minuto, os valores de RU para o grupo 30% de GSE foram maiores do que os demais e mantiveram-se constante pelo período de armazenamento. O efeito dos agentes ricos em PA mostrou ser concentração e tempo dependente, porém uma dentina mais resistente e estável ao longo do tempo é possível de ser obtida, levando-se em conta a estrutura química, conteúdo de PA, solubilidade e outros fatores inerentes ao extrato / The aim of this study was to elucidate the interaction between proanthocyanidin based extracts (PA), a natural and non-toxic cross-linker, and type I dentin collagen. The hypothesis is that if a stronger and more stable collagen layer is chemically engineered, the resultant hybrid layer will be stronger and less prone to degradation. So, this research was divided into four phases: 1) PA validation as an exogenous cross-linking agent, testing the elastic modulus (E) of demineralized dentin treated with various PA based extracts (grape seed-GSE, cocoa seed-CSE, cranberry-CRE, cinnamon-CNE, and açai berry-ACE), exposure times (10, 30, 60, 120, 240 min), and long term effectiveness (immediate, 3, 6 and 12 months); 2) Evaluation of different parameters related to PA solutions, like solubility (water, ethanol and acetone), manufacturer (GSE and CSE) and concentration (0.65%, 3.25% 6.5%, 15% and 30%), E was also obtained by micro-flexural strength measurements of demineralized dentin matrix, 3) Characterization of PA treated dentin (GSE and CSE): water sorption, calorimetry and enzymatic degradation and 4) Analyze the bond strength ( BS) of PA treated dentin (GSE / CSE) with commercial adhesives systems (Adapter Single Bond Plus-SB and One Step Plus-OS), varying concentration (6.5% and 30%), exposure time (1, 10 and 60 min) and long-term effectiveness (immediate, 3, 6 and 12 months). Data were statistically analyzed at a 95% confidence interval. GSE and CSE extracts showed a time-dependant effect and were able to improve and stabilize the E of the organic matrix. CRE and CNE extracts were able to maintain the E of collagen matrices constant after 12 months artifical saliva storage. ACE and controls groups showed no effect over dentin organic matrix, did not prevent degradation and consequently reduced the mechanical properties. Herbal extraction process and other pharmacognostic parameters have an important influence on extract solubility as well as constitution, therefore GSE is better dissolved in water and CSE in acetone-water. GSE effect on demineralized dentin is concentration-dependant, with highest E values at 30% GSE concentration. Dentin treated with PA-based agents was more resistant against enzymatic degradation, less susceptible to water sorption and showed a higher denaturation temperature. As compared to the controls, GSE stabilized the dentin-resin bond strength values over a period of one year for both adhesives, however better results were achieved with SB. CSE showed less predictable results depending on the adhesive system used. One minute treatment with 30% GSE was capable of increasing short-term BS and stabilizing it for at least 6 months. Increased mechanical properties and stability of dentin matrix can be achieved by the use of PA-rich collagen crosslinkers regarding inherent characteristics of the natural compounds as chemical structure, solubility and PA content.
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Investigation of protein-RNA interactions by UV cross-linking and mass spectrometry: methodological improvements toward in vivo applicationsKramer, Katharina 30 May 2013 (has links)
No description available.
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A biomimicking approach for hemicellulose processingOinonen, Petri January 2014 (has links)
Lignocellulose can become the best opportunity for the society to reduce its dependency on the harmful petroleum based products as well as to produce clean energy. In each part of the production cycle, biomass based products have a better environmental profiles than their petroleum based counterparts. Woody biomass has a vast availability, but it suffers from recalcitrance that is mostly caused by lignin that is functioning as a matrix, surrounding and binding the carbohydrates that are currently the most valuable of the wood components. Lignin-carbohydrate (LC) bonds are believed to be a key element in this recalcitrance and research has shown that these types of bonds are common in wood. These bonds are important in an economical point of view as well, as e.g. residual lignin structures in pulp (lignins bonded to the cellulose and hemicelluloses) require expensive bleaching sequences for their removal. The LC-structures can also be exploited technically as we now have demonstrated. We developed a method that utilizes phenolic end groups that are bonded to different hemicelluloses for cross-linking. The enzyme laccase was used for the cross-linking to create a cost-efficient processing scheme to both isolate and increase the molecular weight of the hemicelluloses. Membrane filtration was used as the key separation technique, which enables the establishment of industrial scale production. The final product had improved mechanical and thermal properties and could be used e.g. as barrier film component in renewable packaging. Nanocomposite formation with nanofibrillated cellulose was also studied. This improved the film properties further. The complexes are also possible to use as model compounds for lignin-carbohydrate complexes in wood. This technique can also be seen to mimick the lignification and lignin-carbohydrate network formation phenomena in plants enabling the formation of entire networks of wood components. Our results suggests that the side chains of hemicellulose might play an important role in network formation and that hemicellulose molecules can carry more than one lignin phenolic end group to fulfill this capability. / <p>QC 20140825</p>
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Characterization of the Dynein-Dynactin InteractionFindeisen, Peggy 01 August 2014 (has links)
No description available.
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The role of protein cross-linking in soy food textureMd. Yasir, Suhaimi Bin January 2005 (has links)
Cross-linking in soy proteins is hypothesised to have an impact on the texture of tofu. In vitro incubation showed soy proteins and its two fractions, glycinin and β-conglycinin, were cross-linked using glutaraldehyde, formaldehyde, glyceraldehyde and transglutaminase (TGA). Increasing concentration of these carbonyl compounds and TGA, and temperature of the carbonyl compounds treatment, increased the reactivity of cross-linking. Glutaraldehyde was the most reactive in forming aggregated proteins, followed by formaldehyde and glyceraldehyde. Both carbonyl moieties of glutaraldehyde are believed to be essential for the rapid cross-linking reaction. In the unfractionated soy proteins, β-conglycinin had a higher reactivity than glycinin. In in vitro incubation using TGA, soy proteins served as good substrates for TGA, in which β-conglycinin was more susceptible to TGA than glycinin in the unfractionated soy proteins. The addition of TGA, and 1 and 2 mM glutaraldehyde prior to soymilk boiling in situ resulted in a small number of cross-linked proteins, which correspond to an increase in fracture force. The addition of glutaraldehyde after soymilk boiling resulted in a slight decrease in fracture force compared to the control. At higher concentrations of glutaraldehyde (15 and 30 mM), soy proteins were mostly cross-linked, regardless of addition before or after soymilk boiling. Highly cross-linked proteins resulted in a significant decrease in the fracture force. For TGA treatment, the fracture force was increased with increasing TGA concentration from 1000 to 5000 ppm, added either before or after soymilk boiling. However, the TGA treatment showed only a small quantity of cross-linking. It is hypothesised that TGA hydrolysed glutamine of proteins to glutamate and changed the functional properties of proteins. Upon examination of the microstructure, it was found that the TGA treatment resulted in a fine-stranded network, compact structure and less porosity. These characteristics resulted in a higher fracture force. In contrast, in the glutaraldehyde treatment, the network consisted of a higher porosity, loose network and diffuse structure, which gave lower fracture force. Thus, it appears that substrate modification to the structure of the soy proteins may have a greater impact than the number of cross-links. These findings are likely to have implications for production of soy products with a wide range of textures by manipulating the soy protein properties.
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Topological study of human complement terminal complex /Nuanthip Kamolvarin, Prapon Wilairat, January 1984 (has links) (PDF)
Thesis (Ph.D. (Biochemistry))--Mahidol University, 1984.
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Preparacao de compostos plastico-celulosicos utilizando radiacao gama de alta energiaROSA, MARIA C.F. 09 October 2014 (has links)
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00437.pdf: 935313 bytes, checksum: 06cabc4489d4233743e8951ec8c442ed (MD5) / Dissertacao (Mestrado) / IEA/D / Instituto de Energia Atomica - IEA
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Sintese, caracterizacao e citotoxicidade de hidrogeis polimericos para imobilizacao de farmaco empregado no tratamento de Leihmaniose / Synthesis, characterization and citoxicity of polymeric hydrogels for use to imombilization and drug release on Leishmaniose treatmentOLIVEIRA, MARIA J.A. de 09 October 2014 (has links)
Made available in DSpace on 2014-10-09T12:55:24Z (GMT). No. of bitstreams: 0 / Made available in DSpace on 2014-10-09T14:04:56Z (GMT). No. of bitstreams: 0 / Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) / Dissertação (Mestrado) / IPEN/D / Instituto de Pesquisas Energeticas e Nucleares - IPEN-CNEN/SP / FAPESP:06/53634-3
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