O fenômeno \"crosstalk\" constitui-se de acoplamentos eletromagnéticos não intencionais entre condutores. Motivado pela importância deste fenômeno para a área de Compatibilidade Eletromagnética, este trabalho apresenta uma metodologia de análise do fenômeno através do aplicativo SPICE considerando-se configurações compostas por condutores múltiplos. A proposta de desenvolvimento de uma ferramenta computacional de simulação do fenômeno utilizando uma versão estudantil, com livre acesso, possibilita, entre outras coisas, a disseminação de um modelo alternativo, simples e eficaz, e a inclusão do estudo do \"crosstalk\" no conteúdo programático de cursos de Graduação ou Pós-graduação. Para este fim, adotou-se um modelo de simulação de acoplamento eletromagnético com base em modelos de linha de transmissão. Tal modelo é complementado com geradores vinculados controlados por tensão e corrente, que representam fontes de interferência eletromagnética. Em face de limitações dos blocos funcionais que representam essas fontes no aplicativo, foi utilizado em um primeiro instante um método de representação do modelo através de arquivos-texto, ao invés da utilização da interface gráfica existente no software. Posteriormente, novos blocos foram desenvolvidos e acrescentados à biblioteca, tornando possível à aplicação dessa interface diretamente nas simulações. A validação do modelo foi feita, inicialmente, comparando-se os resultados simulados àqueles disponíveis na literatura internacional. Além disto, foi desenvolvida uma bancada experimental para comprovação dos resultados e validação do modelo implementado no aplicativo SPICE através de casos simples do fenômeno. Esses resultados mostram a viabilidade da utilização dos componentes desenvolvidos para o aplicativo, tornando possível e confiável o uso dessa ferramenta na simulação do fenômeno \"crosstalk\". Passou-se então a aplicação da ferramenta desenvolvida a sistemas mais complexos com um número superior de condutores. / This work presents an approach, in which the well-known SPICE simulator is used as a tool to simulate the phenomenon crosstalk. Basically, it is based on transmission line model, where the electromagnetic interferences are simulated by controlled voltage and current sources. Due to the limitations of the available Spice models concerning multiconductors configurations, additional models were developed and implemented as part of the SPICE library. Theoretical and experimental results were obtained. They were used as part of the validation process of the proposed methodology. The developing of specific models allowed the use of this modeling in SPICE student versions. This turns possible the introduction of crosstalk simulations in undergraduate and graduate courses. Besides, the methodology was applied to complex systems in order to present its versatility.

Master of Science / Department of Electrical and Computer Engineering / William B. Kuhn / An important concern for high speed circuit designs is that of crosstalk and electromagnetic interference. In PCB board-level designs, crosstalk at microwave frequencies may result from imperfections in shielding of PCB interconnects or more generally transmission lines. Several studies have been done to characterize and improve the isolation between PCB transmission lines for both digital and RF circuits. For example, previous studies in the microwave region have examined the effect that line type, line length, and separation have on crosstalk and suggest that without full shielding, the upper limit of isolation is on the order of 60dB for traditional board-level lines [1]. In order to more fully characterize crosstalk and improve isolation above 60 dB, this thesis studies signal-to-ground-plane separation, considers advanced line types, and examines the effect of 3D shielding. Results are presented from 100MHz to 30GHz for the traditional transmission line structures of microstrip, CPW, differential pair and CPW differential pair. This study shows that with a halving of distance between signal and ground planes, isolation between transmission lines can be reduced by as much as 20dB, making this one of the best ways to improve performance. Advanced methods of shielding are then presented. Direct launch stripline and single-sided CPW improve upon existing crosstalk reduction techniques, while split shielding and ablation of dielectric PCB material are also proposed. The data and additional crosstalk reduction techniques discussed in this thesis serve two purposes. One: with a more complete understanding of the effects that transmission line types and parameters have on crosstalk, engineers can quickly identify potential crosstalk issues and resolve them before manufacturing. Second, this thesis presents the engineer with four new additional techniques that may become available in advanced manufacturing environments. Such techniques can further reduce crosstalk and may allow for isolation values to approach 100 dB at the PC board level.

Multiple signaling pathways control cellular response to environmental cues such as nutrients, growth factors and stress. Interpretation of these cues requires coordinated regulation of intracellular signaling pathways. Our attempt to understand how cells coordinate different signaling pathways led to the discovery of two crosstalk mechanisms between different signaling cascades. We found that PI3K-AKT signaling reduces EGFR signaling to the parallel ERK-MAPK pathway by enhancing EGF induced EGFR degradation. At the molecular level AKT activates PIKfyve to facilitate EGFR trafficking from early endosomes to the lysosomes. Using a mass spectrometry based approach we also found growth factor signaling by EGF inhibits stress response. In particular, inhibiting RSK signaling downstream of EGF increased the activity of stress activated kinases p38, MSK2 and ERK5. We propose that when growth factors are present active RSK phosphorylates and inhibits a master regulator of stress response MEKK3, which leads to termination of MEKK3 signaling to downstream kinases. Our unbiased phosphoproteomic approach also lead to identification of many ERK and RSK substrates that will help us explain how growth factor signaling regulates a wide variety of biological processes.

It is a common problem in psychology subject pools for past study participants to inform future participants of key experimental details (also known as crosstalk). Previous research (Edlund, Sagarin, Skowronski, Johnson, & Kutter, 2009) demonstrated that a combined classroom and laboratory treatment could significantly reduce crosstalk. The present investigation tested a laboratory-only treatment for the prevention of crosstalk at five universities, along with institutional-level moderators of crosstalk. Results indicated the presence of crosstalk at all universities and that the laboratory-based treatment was effective in reducing crosstalk. Importantly, crosstalk rates were higher (but successfully neutralized) in research pools with higher research credit requirements. Therefore, this research provides valuable guidance regarding crosstalk prevalence and its minimization by researchers.

When conducting motion analysis using 3-dimensional motion capture technology, errors in marker placement on the knee results in a widely observed phenomenon known as “crosstalk” [1-18] in calculated knee joint angles (i.e., flexion-extension (FE), adduction-abduction (AA), internal-external rotation (IE)). Principal Component Analysis (PCA) has recently been proposed as a post hoc method to reduce crosstalk errors and operates by minimizing the correlation between the knee angles [1, 2]. However, recent studies that have used PCA have neither considered exercises, such as cycling (C) and elliptical training (E), other than gait (G) nor estimated the corrected knee axes following PCA correction. The hypothesis of this study is that PCA can correct for crosstalk in G, C, and E exercises but that subject-specific PCA corrected axes differ for these exercises. Motion analysis of the selected exercises were conducted on 8 normal weight (body mass index (BMI) = 21.70 +/- 3.20) and 7 overweight participants (BMI = 27.45 +/- 2.45). An enhanced Helen Hayes marker set with 27 markers was used to track kinematics. Knee joint FE, AA, and IE angles were obtained with Cortex (Motion Analysis, Santa Rosa, CA) software and corrected using PCA to obtain corrected angles for each exercise. Exercise-specific corrected knee joint axes were determined by finding axes that reproduced the shank and ankle body vectors taken from Cortex when used with the PCA corrected angles. Then, PCA corrected gait axes were used as a common set of axes for all exercises to find corresponding knee angles. Paired t-tests assessed if FE-AA angle correlations changed with PCA. Multivariate Paired Hotelling’s T-Square tests assessed if the PCA corrected knee joint axes were similar between exercises. ANOVA was used to assess if Cortex angles, PCA corrected angles, and knee angles using PCA corrected gait axes were different. Reduced FE-AA angle correlations existed for G (p<0.001 for Cortex and p=0.85 for PCA corrected), C (p=0.01 for Cortex and p=0.77 for PCA corrected), and E (p<0.001 for Cortex and p=0.77 for PCA corrected). Differences in the PCA corrected knee axes were found between G and C (p<0.0014). Then, differences were found between Cortex, PCA corrected, and C and E knee angles using the PCA corrected G axes (p<0.0056). The results of this study suggest that if PCA is used to reduce crosstalk errors in motions other than G then it is recommended to adopt the use of a PCA corrected axes set determined from G to produce the PCA corrected angles.

OBJECTIVE: Obesity is a chronic disease with high incidence worldwide, which promotes the risk of incidence of type 2 diabetes (T2D). Obesity-induced adipocyte expansion promotes local chronic inflammation in the adipose tissue which is considered a contributing factor to insulin resistance, hyperinsulinemia, and T2D. Many organs, including adipose tissue, involve in the dysregulation of glucose homeostasis in T2D. The crosstalk between adipose tissue/adipocytes and pancreatic ß-cells has provoked scientists' interest for years. Here in this thesis, we focused on the effect carried out by adipocyte-specific GPS2 depletion on insulin secretion from pancreatic ß-cells. METHODS: Conditioned media collected over 24 h from both primary adipocyte and adipose tissue explant cultures from high fat diet (HFD)-fed WT and adipocyte-specific GPS2 knock-out (GPS2-AKO) mice were used to treat INS-1 clonal pancreatic ß-cells or primary islets from chow-diet WT mice. Conditioned media was diluted 1:8 in culture media of clonal INS-1 cells (cultured in media with 4 mM or 11 mM glucose chronically) and primary islets (cultured in media with 11 mM glucose) and incubated for 18 h before measuring insulin secretion. The isolated islets from chow-diet WT mice were also be treated with the primary adipocytes conditioned media from eWAT (epididymal white adipose tissue) of HFD-fed WT and GPS2-AKO mice. In addition, the effect of exosomes extracted from primary adipocyte conditioned media of HFD-fed WT and GPS2-AKO mice on GSIS was investigated in clonal INS-1 cells. Glucose-stimulated insulin secretion (GSIS) was measured to assess differences in insulin secretion by INS-1 cells and islets from mice in response to signaling from WT or GPS2-AKO adipocytes. RESULTS: Adipocyte conditioned media from both WT and GPS2-AKO mice reduced GSIS from INS-1 cells by the same extent compared to a non-treated control. The same result was obtained using media conditioned by adipose tissue explant culture. Exosomes isolated from adipocyte conditioned media from both WT and GPS2-AKO mice also reduced GSIS from INS-1 cells with no significant difference between WT and GPS2-AKO. Islets isolated from chow-diet WT mice treated with adipocyte conditioned media from eWAT of WT and GPS2-AKO mice also showed no significant difference between WT and GPS2-AKO in GSIS compared to our non-treated control. CONCLUSIONS: Both conditioned media and exosomes from primary adipocytes of HFD-fed mice inhibits GSIS from INS-1 cells and isolated islets, but no difference was observed between WT and GPS2-AKO mice. We conclude that the deletion of GPS2 in adipocytes does not influence GSIS from pancreatic ß-cells under our experimental conditions. Conditioned media-induced inhibition of GSIS is mediated by factors that may contribute to adipocyte-ß-cell crosstalk in-vivo. / 2025-11-02T00:00:00Z

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This dissertation presents the characterization and modeling of crosstalk noise based on the theory of coupled microstrip lines. An equivalent circuit model used in this work comprises of the addition of mutual inductances and mutual capacitances to the fundamental transmission line model. Characterization of crosstalk noise along adjacent lines, current-voltage characteristics, characteristic impedance, effective dielectric impedance, and maximum crosstalk are performed analytically. Computer simulations and computations of these parameters are also performed. The circuits are realized experimentally, and an investigation of crosstalk noise using time domain and frequency domain measurement techniques is conducted. The results illustrate that the computation matched closely the experimental data and explained the physical phenomena better. / Ph. D.

Autophagy, senescence and apoptosis are tightly linked processes which together determine the fate of cells in response to various stresses. There is ample evidence supporting the notion that senescent cells are highly dependent on autophagy and this process is here much more intensive than in nonsenescent cells. Autophagy may to some extent compensate increased energetic and metabolic demands of senescent cells and also helps with removal of toxic products such as oxidized proteins, protein aggregates and damaged organelles resulting from an overloaded metabolism of some senescent cells. In addition, some studies reported the need of autophagy for the adoption of senescent phenotype. However, there are also studies with seemingly contradictory results claiming that increased autophagy prevents or delays cellular senescence. Relationship of autophagy to apoptosis is similarly ambivalent. Whereas intact autophagy is necessary for the cell, while slightly increased autophagy still has a rather positive impact, excessive autophagy may lead to degradation of critical components necessary for cell function and survival and can trigger one of the modes of programmed cell death. In the first part of this work, we focused on the analysis of autophagic response in senescent and proliferating pancreatic...

Design techniques for heterogeneous three-dimensional (3-D) integrated circuits are developed in this thesis. Heterogeneous 3-D integration is a platform for multifunctional, high performance, and low power electronics. For the advancement of heterogeneous 3-D ICs, contactless solutions are investigated to implement inter-tier communication between tiers manufactured with disparate processes and heterogeneous technologies. Two challenges for the development of contactless inter-tier communication are addressed, the design of energy efficient, heterogeneous inductive link transceivers and the impact of crosstalk noise due to the on-chip spiral inductors. Inter-tier communication between circuits fabricated with disparate technologies requires transceivers capable of operating at dissimilar voltages. A low power transceiver design methodology is proposed exploiting the difference in the core voltage between disparate manufacturing processes in a 3-D system in package. A transceiver is designed to provide inter-tier communication between a sensing layer, designed in a commercial 0.35 Âμm process and a processing layer, designed in an advanced 65 nm process. A significant gain in the power consumed by the transceiver is shown compared to equivalent state-of-the-art prototypes, profiting by the tradeoff between the core voltage and sensing ability of the transceiver circuit in each process. Due to their wireless nature, however the use of inductive links introduces crosstalk noise due to the coupling between the on-chip inductor and on-chip interconnects in the vicinity of the inductor. The noise caused by the inductor on the power distribution network of an integrated system is explored, analysed, and modelled through electromagnetic simulations. The spatial distribution of the noise is described for several power distribution topologies to determine the preferred placement solution for the power and ground network in the vicinity of the inductor, considering the impact on other sources of noise, such as the resistive drop. Depending upon the power distribution network topology, the induced noise can be reduced up to 70% when the additional noise caused by the inductive link is considered by the routing algorithm. Additionally, a methodology utilising an analytic model is proposed for the evaluation of the crosstalk noise without resorting to electromagnetic simulations. A closed-form magnetostatic model is developed to assess the mutual inductance between the on-chip inductor and the power distribution network. Utilising the mutual inductance model, the crosstalk noise is evaluated with SPICE simulations. A signifcant benefit in speedup is achieved, up to four orders of magnitude for determining the mutual inductance and up to 4.7× for the assessment of the crosstalk noise. The accuracy of the model is within 10% of the electromagnetic simulation.