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Polymer linker DNA probe design, characterization and comparison with molecular beaconsMartinez Diaz, Karen. January 2005 (has links)
Thesis (M.S.)--University of Florida, 2005. / Title from title page of source document. Document formatted into pages; contains 47 pages. Includes vita. Includes bibliographical references.
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The genotyping of Neisseria meningitidis by restriction fragment length polymorphism (RFLP) analysisGray, Stephen James January 1996 (has links)
No description available.
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Amplified DNA sequences in malignantly transformed rat ovarian surface epithelial cells : implications in ovarian cancer etiology /Getts, Lori Ann, January 1996 (has links)
Thesis (Ph. D.)--Lehigh University, 1996. / Includes vita. Bibliography: leaves 201-217.
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Detection of Giardia cysts by cDNA probe and application to water samplesAbbaszadegan, Morteza, January 1991 (has links) (PDF)
Thesis (Ph. D. -- Nutritional Sciences) - University of Arizona. / Includes bibliographical references (leaves 126-137).
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Micro bubble actuator for DNA hybridization enhancement /Deng, Peigang. January 2004 (has links)
Thesis (Ph. D.)--Hong Kong University of Science and Technology, 2004. / Includes bibliographical references (leaves 156-165). Also available in electronic version. Access restricted to campus users.
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The detection and identification of nanoflagellates using fluorescent oligonucleotide probesRice, Jason January 1995 (has links)
No description available.
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Development of a DNA probe and anisotropic films with an emphasis on self-assembly and fluorescence /Carson, Travis D. January 2005 (has links)
Thesis (Ph. D.)--University of Nevada, Reno, 2005. / "May, 2005." Includes bibliographical references. Online version available on the World Wide Web. Library also has microfilm. Ann Arbor, Mich. : ProQuest Information and Learning Company, [2005]. 1 microfilm reel ; 35 mm.
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Development of microdevices for applications to bioanalysisKim, Joohoon, 1976- 28 August 2008 (has links)
The development of microdevices for applications related to bioanalysis is described. There are two types of microdevices involved in this study: DNA (or RNA) microarrays and bead-based microfluidic devices. First, a new method to fabricate DNA microarrays is developed: replication of DNA microarrays. It was shown that oligonucleotides immobilized on a glass master can hybridize with their biotin-modified complements, and then the complements can be transferred to a streptavidinfunctionalized replica surface. This results in replication of the master DNA array. Several innovative aspects of replication are discussed. First, the zip code approach allows fabrication of replica DNA arrays having any configuration using a single, universal master array. It is demonstrated that this approach can be used to replicate master arrays having three different sequences (spot feature sizes as small as 100 [mu]m) and that master arrays can be used to prepare multiple replicas. Second, it is shown that a surface T4 DNA polymerase reaction improves the DNA microarray replication method by removing the requirement for using presynthesizd oligonucleotides. This in-situ, enzymatic synthesis approach is used to replicate DNA master arrays consisting of 2304 spots and arrays consisting of different oligonucleotide sequences. Importantly, multiple replica arrays prepared from a single master show consistent functionality to hybridization-based application. It is also shown that RNA microarrays can be fabricated utilizing a surface T4 DNA ligase reaction, which eliminates the requirement of modified RNA in conventional fabrication schemes. This aspect of the work shows that the replication approach may be broadly applicable to bioarray technologies. A different but related aspect of this project focuses on biosensors consisting of microfluidic devices packed with microbeads conjugated to DNA capture probes. The focus here is on understanding the parameters affecting the hybridization of DNA onto the probeconjugated microbeads under microfluidic flow conditions. These parameters include the surface concentration of the probe, the flow rate of the solution, and the concentration of the target. The simple microfluidic device packed with probe-conjugated microbeads exhibits efficient target capture resulting from the inherently high surface-area-to-volume ratio of the beads, optimized capture-probe surface density, and good mass-transfer characteristics. Furthermore, the bead-based microchip is integrated with a hydrogel preconcentrator enhancing the local concentration of DNA in a icrochannel. The integration of the preconcentrator into the bead-based capture chip allows significantly lower limit of detection level (~10-fold enhancement in the sensitivity of the microbeadbased DNA detection). / text
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Probing the structural and electronic properties of deoxyribonucleic acids with anthraquinone photonucleasesKan, Yongzhi 05 1900 (has links)
No description available.
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Mechanism of electron transfer in double-stranded DNA and PNA-DNA hybrids, and the development of a fluorescence probe for DNA and RNA detectionLy, Danith 12 1900 (has links)
No description available.
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