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abstract: A major goal of synthetic biology is to recapitulate emergent properties of life. Despite a significant body of work, a longstanding question that remains to be answered is how such a complex system arose? In this dissertation, synthetic nucleic acid molecules with alternative sugar-phosphate backbones were investigated as potential ancestors of DNA and RNA. Threose nucleic acid (TNA) is capable of forming stable helical structures with complementary strands of itself and RNA. This provides a plausible mechanism for genetic information transfer between TNA and RNA. Therefore TNA has been proposed as a potential RNA progenitor. Using molecular evolution, functional sequences were isolated from a pool of random TNA molecules. This implicates a possible chemical framework capable of crosstalk between TNA and RNA. Further, this shows that heredity and evolution are not limited to the natural genetic system based on ribofuranosyl nucleic acids. Another alternative genetic system, glycerol nucleic acid (GNA) undergoes intrasystem pairing with superior thermalstability compared to that of DNA. Inspired by this property, I demonstrated a minimal nanostructure composed of both left- and right-handed mirro image GNA. This work suggested that GNA could be useful as promising orthogonal material in structural DNA nanotechnology. / Dissertation/Thesis / Ph.D. Chemistry 2011

Dans le cadre d’explications darwiniennes, il est nécessaire de référer minimalement à deux niveaux d’organisation biologique, soit celui de l’individu et celui de la population. Dans ce mémoire, nous cherchons à fournir des outils pour distinguer ces deux niveaux et, du même coup, ces deux concepts. À la lumière des travaux de Millstein (2010b) et de la conception de l’individualité défendue par Hull (1978) et Ghiselin (1974), nous proposons un continuum de l’individualité pensé en termes d’intégration sur lequel se situent les individus et les populations. Les populations sont associées à un faible degré d’intégration alors que les individus présentent un degré d’intégration élevé. En adoptant la méthodologie des paramètres gradients de Godfrey-Smith (2009b), la distinction catégorique entre les deux concepts est repensée comme étant une distinction graduelle. Dans ce mémoire, nous explorons les fondements pragmatistes de cette méthode et argumentons pour son utilisation en philosophie de la biologie. / Darwinian explanations require the distinction between two levels of biological organisation, that of the individual and that of the population. This research aims at identifying tools to distinguish these two levels and the related concepts. Building on Millstein’s work concerned with the notion of population (2010b) and on the theory of individuality defended by Hull (1978) and Ghiselin (1974), we propose a continuum of individuality understood in terms of integration and on which figure both populations and individuals. The population concept is associated with a low degree of integration, while the notion of individuality refers to a high degree. Such a continuum of individuality relies on a specific philosophical standpoint associated with Godfrey-Smith’s gradient parameters methodology (2009b). Our research will explore the pragmatist foundations of this methodology in order to argue for its use in philosophy of biology.